Articles

Found 16 Documents
Search

Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; S, Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.993 KB)

Abstract

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.
T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1 Da’i, Muhammad; Jenie, Umar Anggara; AM, Supardjan; Kawaichi, Masashi; Meiyanto, Edy
Indonesian Journal of Biotechnology Vol 12, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.077 KB)

Abstract

its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21
PENGARUH LOKASI TUMBUH, UMUR TANAMAN DAN VARIASI JENIS DESTILASI TERHADAP KOMPOSISI SENYAWA MINYAK ATSIRI RIMPANG Curcuma mangga PRODUKSI BEBERAPA SENTRA DI YOGYAKARTA Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; Sismindari, Sismindari
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 21, No 3 (2014)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1.84 KB)

Abstract

Minyak atsiri rimpang Curcuma mangga telah diketahui bersifat toksik terhadap beberapa jenis sel kanker. Toksisitas minyak atsiri dipengaruhi oleh komposisi senyawa penyusunnya. Komposisi minyak atsiri dipengaruhi oleh berbagai faktor ekologi dan metode isolasi. Penelitian ini bertujuan untuk mengetahui pengaruh lokasi tumbuh, umur tanaman dan variasi jenis destilasi terhadap komposisi senyawa minyak atsiri rimpang C. mangga. Untuk mempelajari pengaruh lokasi tumbuh diambil rimpang C. mangga yang berasal dari beberapa daerah di Daerah Istimewa Yogyakarta. Pengaruh umur tanaman terhadap komposisi senyawa dalam minyak atsiri digunakan rimpang C. mangga umur 1, 2, 3, 7, 10, 11 dan 12 bulan, sedangkan jenis destilasi yang digunakan adalah destilasi uap dan uap air. Analisis komponen senyawa minyak atsiri digunakan Kromatografi Gas-Spektrometer Massa (KG-SM). Hasil penelitian menunjukkan bahwa rimpang yang berasal dari daerah dataran rendah memiliki jenis senyawa yang lebih banyak daripada dataran tinggi dan dimungkinkan karena curah hujan di dataran rendah lebih kecil daripada dataran tinggi. Minyak atsiri rimpang C. mangga optimum apabila tanaman dipanen pada saat umur 8-10 bulan dan jenis destilasi berpengaruh terhadap komposisi minyak atsiri.  Namun begitu, senyawa utama minyak atsiri rimpang C. mangga untuk semua variasi perlakuan adalah sama yaitu β-osimen, β-pinen, β-mirsen dan p-sineol. 
Geometric Isomers and Cytotoxic Effect On T47D Cells of Curcumin Analogues PGV-0 and PGV-1 Meiyanto, Edy; M., Supardjan A.; Jenie, Umar Anggara
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 1, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.101 KB) | DOI: 10.14499/indonesianjpharm0iss0pp40-47

Abstract

Curcumin analogues 2,5-bis-(4’-hidroxy-3’-methoxy)-benzilidinecylopentanone (PGV-0) and 2,5-bis-(4’hidroxy-3’,5’-dimethyl)-benzilidinecylopentanone (PGV-1) have a potency to be developed as cytotoxic agent. The aims of this research are to elucidate the geometric isomer and to study the cytotoxic effect on T47D cells of both compounds. To establish the geometric isomer these compounds, they were elucidated by LC-MS, 1H-NMR, 13C-NMR, HMBC, HMQC, NOESY. Their cytotoxic effect were evaluated by MTT assay method on T47D cells. The results concluded that the geometric isomer of PGV-1 is zusammen-zusammen (Z-Z) and PGV-0 is entgegen-entgegen (EE). The IC50 of both compounds are 1.74 and 9.39 μM respectively.Key words: PGV-0, PGV-1, Cytotoxicity
Production of D6,7-anhidroeritromisin-A from Saccharopolyspora erythraea ATCC 11635 culture ., Khairan; Jenie, Umar Anggara; Sudibyo, Retno S.
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.689 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-205

Abstract

Erythromycin has been used widely to prevent infection diseases which caused by Staphylococcus. However erythromycin is unstable and decomposed in an acid condition. This unstability erythromycin is conducted by due to a nucleophylic attack of the C6-hydroxyl group of erythromycin to its C9-carbonyl group. This Decomposition can be avoided by modification the erythromycin structure; such as omitting the C6-hydroxyl group. Biomodification for omitting the C6-hydroxyl group can be conducted by inhibition the activity of enoyl reductase in fourth step of the biosynthesis 6- deoxyerythronolid-B. The inhibition process could carried out by an addition of an antimetabolite isonicotonic hidrazide (INH) into the fermentation of erythromycin production. By the enoyl reductase inhibition, the microbe will produce D6,7-Anhydroerythromycin-A which is more stable in an acidcondition than erythromycin-A. This research is to produce derivative D6,7-Anhydroerythromycin-A by addition of INH into a culture of Saccharopolyspora erythraea ATCC 11635 in medium basal and Hutchinson. Selection of medium for fermentation of Sac. erythraea ATCC 11635 for D6,7-Anhydroerythromycin-A production was done out of two media: basal medium with palm oil and Hutchinson medium. The two media were treated with an additional 0,2% INH as an antimetabolite.Hutchinson medium yielded the highest product of D6,7-Anhydroerythromycin-A. The FT-IR spectrometric analyzes of metabolite showed a stretching vibration of C=C conjugated group at wave number 1602,7 cm-1. This C=C conjugated vibration indicated the existence of double bond between C6 and C7 (D6,7), this confirmed that isolate-C contained D6,7-Anhydroerythromycin-A (the possibility of D6,7 was positive).Key words: enoyl reductase, D6,7-anhydroerythromycin-A, isoniazid (INH)
Isolation of cytotoxic substance from Kaliapsis sponge Setyowati, Erna Prawita; Jenie, Umar Anggara; ., Sudarsono; Kardono, Broto; Rahmat, Rachmaniar; Meiyanto, Edy
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 4, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.384 KB) | DOI: 10.14499/indonesianjpharm0iss0pp183-189

Abstract

An isolation of cytotoxic substance of Kaliapsis sponge has been conducted. The substance was isolated using maceration, partition, vacuum liquid chromatography and preparative thin layer chromatograpy methods.The result of research showed that peak 1 has the most cytotoxic activity. Cytotoxicity test with MTT [3 (4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide] reagen on myeloma cell showed that the peak 1 had a high activity against myeloma cell. It has IC50 equal to 0,18 μg/mL.Key word: Kaliapsis sponge, cytotoxic, myeloma cell
Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; Sismindari, S.
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.993 KB) | DOI: 10.22146/ijbiotech.8631

Abstract

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.
MOLECULAR DOCKING OF D6-ANHYDROERYTHROMYCIN TO rRNA 23S Deinococcus radiodurans AND THE PREDICTION OF ITS ANTIBIOTIC POTENCY Haryadi, Winarto; Jenie, Umar Anggara; Sudibyo, Retno Sunarminingsih; Pranowo, Harno Dwi; Wibowo, Fajar Rakhman
Indonesian Journal of Chemistry Vol 9, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.856 KB) | DOI: 10.22146/ijc.21546

Abstract

D6-anhidroeritromisin-A is a new derivative of erythromycin which is synthesized through biosynthetic engineering technique. The molecular docking in rRNA 23S Deinoccocus radiodurans are accomplished to determine the model and strength of binding to the target macromolecule. The molecular docking of erythromycin-A and 6-deoksieritromisin-A to the same macromolecule is used as a control. The docking result of the D6-anhidroeritromisin-A shows that it occupies the same cavity as of the experimental erythromycin-A in the same macromolecule. The binding position of D6-anhidroeritromisin-A is not exactly same as erythromycin-A and 6-deoksieritromisin-A due to the presence of D6 unsaturated double bond. However the hydroxyl group(OH) at C-6 does not have an apparent effect on the binding model to rRNA23S D. radiodurans.    Keywords: D6-anhidroeritromisin-A, rRNA 23S D. radiodurans, molecular docking, antibiotic potency
Toksisitas dan Aktivitas Anti-mikroba Ekstrak Etanol Bunga Karang dari Perairan Pulau Tabuhan Banyuwangi dan Pulau Menjangan Bali Barat Setyowati, Erna Prawita; Jenie, Umar Anggara; Sudarsono, Sudarsono; Kardono, Broto
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.26

Abstract

The purpose of the research were to determine the toxicity and antimicrobial activity of ethanolic extract of sponges collected from Tabuhan Island, Banyuwangi and Menjangan Island, West Bali. Extracts were made by maceration of the sponge samples for 3 x 24 hours. The toxicity test was carried out by Brine Shrimp Lethality Test (BST) with 48 hours-old Artemia sp. The effect of ethanolic extract was identified by determining the mortality of Artemia sp. and LC50 of each extract was analyzed by using probit analysis. Antimicrobial activity was tested by agar diffusion method. The result showed that several ethanolic extracts exhibited toxicity to Artemia sp. The toxicity test showed that extract of sponge code B43 was the most toxic with the LC50 of 8.4 µg/ml. Antimicrobial activity test exhibited that ethanolic extract of sponge with code numbers B2, B57, B61 dan W5 were active against Candida ablicans, while the axtracts of sponge with code numbers B9, B35, B47, B51 (Liosina sp.), B57, B61, B63 and W11 were active against Staphylococcus aureus. One ethanolic extract from sponge with code number B57 exibited activity against Eschericia coli.
T47D cells arrested at G2M and Hyperploidy Formation Induced by a Curcumin’s Analogue PGV-1 Da’i, Muhammad; Jenie, Umar Anggara; AM, Supardjan; Kawaichi, Masashi; Meiyanto, Edy
Indonesian Journal of Biotechnology Vol 12, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.077 KB) | DOI: 10.22146/ijbiotech.7776

Abstract

its chemical structure than curcumin. As a curcumin analogue, PGV-1 was considered to have anticanceractivities. This research was conducted to study the effect of PGV-1 on the cycle progression of T47D cells. Cytotoxiceffects of PGV-1 on T47D cells were determined using MTT assay, and the the effect on cell cycle progressionwas carried out using flowcytometry. Western blot analysis was used to analyze protein expression correspondingto cell cycle progression. The result showed that at the concentration of 2.5 μM PGV-1 inhibited cell cycleprogression through G2/M arrest and induced of cells hyperploidy formation. The hyperploidy formation inducedby PGV-1 was related to the increase of cdc-2 expression. PGV-1 2.5 μM elevated the level of p21 CIP/KIPthrough p53- independent manner. Apoptosis was also induced by PGV-1 at early phase of treatment indicated byPARP cleavage due to activation of caspase-3/7 after 12 h treatment. The results above suggest that PGV-1 inhibitsthe growth of T47D cells targeted on microtubules.Keywords: PGV-1, G2/M arrest, apoptosis, p21