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EFFECT OF THYROXINE HORMONE BY ORAL ON GROWTH AND SURVIVAL RATE OF CORAL PLATY XIPHOPHORUS MACULATES Junior, M. Zairin; Pahlawan, R.G.; Raswin, M.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.771 KB) | DOI: 10.19027/jai.4.31-35

Abstract

Thyroxine is known as a hormone that can affect growth of fish by increasing the metabolic rate, feed efficiency and protein retention.  In this study, effect of thyroxine administration through feed on growth and survival of coral platy fish (Xiphophorus maculatus) was observed.  Dosage of thyroxine used was 0.2, 2 and 20 mg/kg of feed, and were combined with duration time of treatment i.e. 1, 2 and 3 weeks.  All treatments were performed triplicate.  The results of study show that higher growth in length (21.3 mm) and weight (244.5 mg) was obtained by administration of 20 mg thyroxine per kilogram of feed for two weeks and this treatment had no effect on the survival rate of coral platy fish Keywords: coral platy, Xiphophorus maculatus, tyroxine, growth   ABSTRAK Tiroksin diketahui sebagai hormon yang dapat mempengaruhi pertumbuhan dengan jalan meningkatkan laju metabolisme tubuh, efisiensi makanan dan retensi protein. Pada penelitian ini dipelajari pengaruh pemberian hormon tiroksin melalui pakan terhadap pertumbuhan dan kelangsungan hidup ikan plati koral (Xiphophorus maculatus). Dosis tiroksin yang digunakan yaitu 0,2; 2 dan 20 mg/kg pakan, dan dikombinasikan dengan lama waktu perlakuan satu, dua dan tiga pekan. Semua perlakuan diulang tiga kali. Hasil penelitian menunjukkan bahwa pemberian hormon tiroksin sebesar 20 mg/kg pakan selama dua pekan memberikan pertumbuhan panjang dan pertambahan berat terbaik masing-masing 21,3 mm dan 244,5 mg dan tidak berpengaruh terhadap kelangsungan hidup ikan platy koral. Kata kunci: ikan plati koral, Xiphophorus maculatus, tiroksin, pertumbuhan
EFFECT OF TRIIODOTHYRONINE IN DIFFERENT DOSAGES ON GROWTH AND SURVIVAL RATE OF GIANT GOURAMY (OSPHRONEMUS GOURAMY LAC.) Herviani, I.; Junior, M. Zairin; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.151 KB) | DOI: 10.19027/jai.2.61-65

Abstract

This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.   ABSTRACT This experiment was conducted to study the effect of giant gouramy larval immersion in triiodothyronine (T3) hormone solution on their growth and survival rate. One-day old larvae were treated with different concentrations of triiodothyronine (T3) i.e. 0; 0,001; 0,01; 0,1 and 1 ppm by immersion method for 24 hours. During eight weeks rearing period, larvae were fed to satiation with Tubifex three times daily. The media were aerated and changed 10-30% daily. At the end of experiment, there was no significant difference in yolk sac absorption between treated larvae and control. The highest dose of 1 ppm T3 resulted significant decreased in total length, average weight and survival rate of larvae. Key Word : Giant gouramy, Osphronemus gouramy, triiodothyronine, immersion dose, growth, survival rate.
EFFECT OF EXPOSURE TIME OF TRIIODOTHYRONINE (T3) HORMONE SOLUTION ON DEVELOPMENT, GROWTH AND SURVIVAL RATE OF GIANT GOURAMY (OSPHRONEMUS GOURAMY LAC.) Sakdiah, M.; Junior, M. Zairin; Carman, O.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.502 KB) | DOI: 10.19027/jai.2.1-6

Abstract

ABSTRACTThe objectives of this research were carried out to determine exposure time of giant gouramy larvae in triiodothyronine (T3) hormone solution on development, growth and survival rate. One-day old larvae were immersed in 0,1 ppm T3 hormone solution for 0, 2, 4, 6, 8, 16, and 24 hours. Results showed that treated larvae developed faster than control larvae. At first, second, fifth, sixth and seventh week, larvae that immersed in T3 hormon solution had total length longer than that of control. Treated larvae had average body weight heavier than that of control until seven weeks of experiment. Immersion of larvae for 16 hours gave the best result in term of length and average body weight. The best survival rate of larvae were obtained from 8 hours treatment.Key words :  Giant gouramy larvae, triiodothyronine, exposure time, growth and survival rate.    ABSTRAKPenelitian ini dilakukan untuk menentukan lama perendaman di dalam larutan hormon triiodotironin (T3) terhadap perkembangan, pertumbuhan dan kelangsungan hidup larva ikan gurame. Larva ikan yang berumur satu hari direndam dalam larutan hormon triiodotironin 0,1 ppm dengan lama perendaman  0, 2, 4, 6, 8, 16, dan 24 jam. Perkembangan larva yang diberi perlakuan T3 lebih cepat daripada perkembangan larva kontrol. Larva yang direndam dalam larutan hormon T3 lebih panjang daripada kontrol pada minggu ke-5, 6 dan 7. Bobot rata-rata larva perlakuan lebih besar daripada bobot rata-rata kontrol dari minggu awal sampai minggu ke-7. Perendaman larva selama 16 jam memberikan hasil terbaik dari segi panjang total dan bobot rata-rata. Nilai kelangsungan hidup terbaik di akhir penelitian diperoleh pada perendaman selama 8 jam. Kata kunci: Larva gurame, triiodotironin, lama perendaman, pertumbuhan dan kelangsungan hidup.
MASCULINIZATION OF NILE TILAPIA (OREOCHROMIS NILOTICUS) BY ADMINISTRATION OF BULL TESTES MEAL Muslim, Muslim; Junior, M. Zairin; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.672 KB) | DOI: 10.19027/jai.10.51-58

Abstract

The synthetic steroid 17?-Methyltestosteron (MT) is commonly used as a feed additive to produce male population of Nile tilapia (Oreochromis niloticus). The use of synthetic testosterone hormone is not recommended in Indonesia. This study was conducted to evaluate the effect of natural testosterone hormone in bull testes meal (BTM) on the masculinization of Nile tilapia using validated aceto carmine squash method of gonads of the fish. Experimental design was utilized two factors experiments in completely randomized design. Fry kept in 40-L glass aquaria at a density of 40 fry/aquarium. Fry (7 dph) received the BTM  for 7 days (T1), 14 days (T2) and 21 days (T3) and doses 0% (D1), 3% (D2), 6% (D3), and 9% (D4).  When treatment was these results, indicated that significant (P?0.05) masculinization occurred only in the group treated of BTM and no treated of BTM. In the group treated of BTM, doses and duration treatment is not significant. The percentage of male fish 83.3% (9%-7d, 9%-21d, 6%-21d: doses and duration, respectively), higher than all group. Survival rate of fry (95-99.5%) is not affected by treatment BTM (no significant P?0.05). Fish growth was significantly affected by treatment BTM compare with no treated of BTM. The highest growth performance of fry were obtained with the 9% BTM.Key words: masculinization, nile tilapia, bull testes meal ABSTRAKSteroid sintetik 17?-Methyltestosteron (MT) umumnya digunakan sebagai aditif pakan untuk menghasilkan populasi ikan nila jantan (Oreochromis niloticus). Penggunaan hormon testosteron sintetis tidak dianjurkan di Indonesia. Penelitian ini dilakukan untuk mengevaluasi pengaruh hormon testosteron alami dalam makanan testis banteng (BTM) pada maskulinisasi ikan nila menggunakan metode divalidasi aceto carmine squash, dari gonad ikan. Desain eksperimental dimanfaatkan dua eksperimen faktor dalam desain benar-benar acak. Fry disimpan dalam 40-L akuarium kaca pada kepadatan 40 fry/akuarium. Fry (7 DPH) menerima BTM selama 7 hari (T1), 14 hari (T2) dan 21 hari (T3) dan dosis 0% (D1), 3% (D2), 6% (D3), dan 9% (D4). Ketika pengobatan hasil ini, menunjukkan signifikan (P ? 0,05) hanya terjadi maskulinisasi pada kelompok perlakuan dari BTM dan tidak diperlakukan BTM. Pada kelompok diobati BTM, dosis dan durasi pengobatan tidak signifikan. Persentase ikan jantan 83,3% (9%-7d, 9%-21d, 6%-21d: dosis dan durasi, masing-masing), lebih tinggi dari kelompok semua. Tingkat kelangsungan hidup benih (95-99,5%) tidak dipengaruhi oleh pengobatan BTM (tidak ada P yang signifikan ? 0,05). Pertumbuhan ikan secara signifikan dipengaruhi oleh BTM pengobatan dibandingkan dengan tidak diobati BTM. Kinerja pertumbuhan tertinggi fry diperoleh dengan BTM 9%.Kata kunci: maskulinisasi, ikan nila, tepung testis sapi 
PORTRAIT OF HATCHERY MANAGEMENT PROFILE ON RAINBOW FISH MELANOTAENIA BOESEMANI (ALLEN & CROSS, 1980) CULTIVATION IN JAKARTA AREA Nugraha, Media Fitri Isma; Avarre, Jean-Christophe; Pouyaud, Laurent; Kadarusman, Kadarusman; Carman, Odang; Junior, M. Zairin
TORANI: Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i2.4484

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Hatchery managers and maintaining genetic diversity and fitness population in endangered and threatened species in pond is a important and difficult thing to do.  But is must to do for conservation biology.  Fitness and structure population depends on effective breeding number (Ne) and population connectivity between each other. The second most important thing is gene flow and genetic drift. Ne is major role in the maintenance of genetic diversity as indicator for inbreeding depresion and genetic drift.  We sampled 6 hatchery in Jakarta  and Bekasi Indonesia region and used 12 polymorphic microsatellite loci to quantify population genetic structure. Result in this study are, each farm have different methode. The old farmers is bapak Gusi and bapak Hasan (+30 years).  Ne in bapak Hasan and bapak Gusi hatchery is 66,667 and F value is 0,00749 and loosing allele (P) is 0,26183.  Compared with younger farmers bapak Yahya (5 years) have Ne = 133,333, F = 0,00375 and P = 0,06855.  Even though hatchery bapak Hasan and bapak Gusi have the same value Ne, F and P but the result in population structure they are different founder populations. Hatchery bapak Hasan have unique structure and alleles composition compared with other hatchery.Keywords: breeding, management, Melanotaenia boesemani, hatchery, population.
FEEDING BROODSTOCK ON A DIET CONTAINING VITAMIN E AND FISH OIL IMPROVE EGGS AND LARVAL QUALITY OF NILE TILAPIA (OREOCHROMIS NILOTICUS) Darwisito, S.; Junior, M. Zairin; Sjafei, D.S.; Manalu, W.; Sudrajat, A. Oman
Jurnal Akuakultur Indonesia Vol. 7 No. 1 (2008): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.407 KB) | DOI: 10.19027/jai.7.1-10

Abstract

The experiment was conducted to determine the effect of dietary vitamin E and n-3 fatty acids on the gonad maturation, egg and larva quality of Nile tilapia. Fish were treated by various combinations of dietary dosage of vitamin E (50, 100, 150, and 200 mg/kg feed) and fish oil (10, 20, 30, and 40 g/kg feed). Three hundreds and twenty pairs of broodstock fish (female at stage of maturity II) were selected and used for this experiment. Fish were fed on the experimental diets three times a day at satiation. The tested of parameters such as gonad somatic index, egg diameter, fecundity, number of spawned fish, hatching rate, and survival rate. Results of the experiment indicated that supplementation of vitamin E and fish oil stimulated gonad development and increased fecundity, hatching rate and survival rate of Nile tilapia larva. Combination of vitamin E 150 mg/kg feed and fish oil 30 g/kg feed significantly improved egg and larva quality of Nile tilapia. Thus, combination of vitamin E in 150 mg/kg diet with fish oil in dose 30 g/kg diet give the best reproduction performance. Keywords: Nile tilapia,  fish oil, vitamin E, reproduction performance   ABSTRAK Penelitian ini dilakukan untuk mendapatkan kadar kombinasi vitamin E dan asam lemak esensial n-3 untuk pematangan gonad, kualitas telur dan larva ikan nila. Adapun dosis dari masing-masing perlakuan terdiri dari 4 taraf. Untuk vitamin E yaitu: 50 mg/kg, 100 mg/kg, 150 mg/kg,  dan 200 mg/kg pakan  sedangkan minyak ikan yaitu: 10 g/kg, 20 g/kg, 30 g/kg dan 40 g/kg pakan. Ikan uji yang digunakan sebanyak 320 ekor induk betina dan 320 ekor induk jantan dan diseleksi.  Setiap hari ikan diberi pakan uji 2 kali sehari (pagi dan sore) secara at satiation. Selama periode pemeliharaan parameter yang diamati meliputi: indeks gonad somatik, diameter telur, fekunditas, jumlah induk yang memijah, derajat tetas telur, dan ketahanan hidup larva. Hasil penelitian ini menunjukkan bahwa pakan dengan kombinasi dosis vitamin E 150 mg/kg dan minyak ikan 30 g/kg memberi respons terhadap perkembangan gonad, fekunditas, derajat tetas telur dan ketahanan hidup larva ikan nila.  Kombinasi vitamin E 150 mg/kg dan minyak ikan 30 g/kg pakan memberi pengaruh signifikan dalam meningkatkan kualitas telur dan larva ikan nila. Dengan demikian, kombinasi vitamin E 150 mg/kg dan minyak ikan 30 g/kg pakan adalah memberi hasil terbaik pada performa reproduksi. Kata kunci: ikan nila, minyak ikan, vitamin E, performa reproduksi
PAIR REPLACEMENT ON THE SPAWNING SUCCESS OF BROODSTOCK SEAHORSE (HIPPOCAMPUS BARBOURI) Syafiuddin, .; Junior, M. Zairin; Jusadi, Dedi; Carman, Odang; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1111.033 KB) | DOI: 10.19027/jai.10.29-37

Abstract

Seahorse, (Hippocampus barbouri) is one of marine living resources having high commercial values and has commonly been traded especially as live ornamental aquarium fish, raw material of traditional medicine and as souvenirs. This expriment was conducted to determine the succces of spawning rate by replacing the broodstock pair of seahorse. This study was done experimentally with treatment of replacement of broodstock pair after spawning under control condition. The experiment was designed to apply completely randomize design by using the following treatments: Treatment A, without replacement neither male nor female. Treatment B, spawned female broodstock  was being mated with her unpaired male broodstock.  Treatment C, a male broodstock that still brood was being mated with his unpaired female broodstock.  Treatment D, a spawned male broodstock that has released larva was being mated with his unpaired female broodstock.  Results showed that under control condition the replacement of broodstock pairs of seahorse had significantly influenced the spawning interval, number of eggs released and number of juveniles produced (P0,05).  It can be concluded that seahorse is not monogamous, either male or female after being spawned may accept other pair for the next spawning. Key words: pair replacement, broodstock, success spawning, Hippocampus barbouri   ABSTRAK Kuda laut, (Hippocampus barbouri) merupakan salah satu sumberdaya hayati laut yang memiliki nilai komersial dan telah banyak diperdagangkan terutama sebagai ikan hias, bahan baku obat tradisional dan juga sebagai suvenir. Penelitian ini dilakukan dengan tujuan untuk mengkaji tingkat keberhasilan pemijahan dengan penggantian pasangan induk kuda laut pada wadah budidaya. Percobaan ini dilakukan secara ekperimental dengan perlakuan penggantian pasangan induk setelah pemijahan dalam wadah budidaya. Percobaan dirancang dengan menggunakan rancangan acak lengkap (RAL) dengan perlakuan sebagai berikut: Perlakuan  A, pemijahan   sepasang  induk kuda laut (tanpa pergantian). Perlakuan B, pemijahan induk betina yang telah memijah dengan induk jantan bukan pasangannya. Perlakuan C, pemijahan  induk  jantan  yang telah memijah (mengerami telur) dengan induk betina bukan pasangannya. Perlakuan D, pemijahan induk jantan yang telah melahirkan dengan induk betina bukan pasangannya. Hasil penelitian menunjukan bahwa penggantian pasangan induk pada wadah budidaya sangat berpengaruh terhadap interval pemijahan, jumlah telur yang dikeluarkan dan jumlah juwana yang dihasilkan (P0,05). Dari hasil penelitian ini disimpulkan bahwa kuda laut, tidak bersifat monogami, artinya baik jantan maupun betina setelah memijah dapat menerima pasangan lain untuk pemijahan berikutnya. Kata kunci: induk, keberhasilan pemijahan, pergantian pasangan, Hippocampus barbouri
STUDY OF GONADOTROPIN (GTH) STIMULATING HORMONE ON GONAD MATURATION OF CLIMBING PERCH ANABAS TESTUDINEUS BLOCH Suriansyah, .; Junior, M. Zairin; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.913 KB) | DOI: 10.19027/jai.9.61-66

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Gonadotropin can quicken the process of 17?-hidroxyprogesterone hormone synthesis becoming 17?, 20?-di hidroxyprogesterone as the maturation inducing steroids (MIS) and quicken the process of egg nucleus integration to germinal vesicle breakdown (GVBD) position. This research aimed to know the efficacy of gonadotropin hormone in the form of ovaprim on gonad maturation of climbing perch (Anabas testudineus Bloch). Stimulation of GtH with a dose of 0.5 ml/kg of fish body weight could improve the fish gonado somato index (GSI) to 2.72 %, improve the the final egg diameter to  0.70 mm (71.50 %), and shorten ovulation time which down to 4.30 hours. Key words: Gonadotropin hormone, gonad maturation, Anabas testudineus   ABSTRAK Hormon gonadotropin dapat mempercepat proses sintesa hormon 17?-hidroksiprogesteron menjadi 17?, 20?-dihidroksiprogesteron yang berfungsi sebagai steroid yang merangsang pematangan gonad dan mempercepat proses integrasi inti sel telur menuju posisi germinal vesicle breakdowan (GVBD). Penelitian ini bertujuan untuk mengetahui efektivitas hormon gonadotropin yang terdapat dalam ovaprim terhadap pematangan gonad ikan betook Anabas testudineus Bloch. Pemberian hormon GtH dalam bentuk ovaprim dengan dosis 0,5 ml/kg bobot ikan dapat memperbaiki perkembangan gonad yang ditunjukkan dengan peningkatan nilai gonado somatik indeks (GSI) sebanyak 2,72%, peningkatan diameter telur menjadi 0,77 mm (71,50%) dan mempercepat waktu ovulasi menjadi 4,3 jam. Kata-kata kunci: Hormon gonadotropin, pematangan gonad, ikan betok
REPRODUCTION PATTERN AND MULTISPECIFIC SPAWNING OF ACROPORA SPP. IN SPERMONDE ISLANDS REEF, INDONESIA (POLA REPRODUKSI DAN PEMIJAHAN MULTISPECIFIC ACROPORA SPP. DI KEPULAUAN SPERMONDE, INDONESIA) Yusuf, Syafyudin; Jompa, Jamaluddin; Zamani, Neviaty P.; Junior, M. Zairin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 3 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.874 KB) | DOI: 10.14710/ik.ijms.18.3.172-178

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Perairan laut tropis seperti di Indonesia yang memiliki variasi lingkungan yang hampir konstan? diduga periode pemijahan karang melebar sampai beberapa bulan dan pada fase bulan yang berbeda, sehingga sulit menentukan waktu pemijahannya dalam skala bulan, hari dan jam. Penelitian ini akan memberikan informasi pola reproduksi dan sinkronisasi pemijahan beberapa jenis karang Acropora spp di Kepulauan Spermonde, Makassar. Sebanyak tujuh jenis karang Acropora spp. diamati kematangan atau kemunculan gonad dan pemijahannya di alam (in situ) dan atau di laboratorium (ex situ) di Marine Station Universitas Hasanuddin. Pola reproduksi menunjukkan spawning berlangsung setiap musim hujan pada bulan Februari-Maret selama tiga tahun berturut-turut. Pemijahan berlangsung secara sinkron dan broadcasting pada awal bulan purnama (0 BP sampai +2 BP), pada jam 18:10?19:00). Bersamaan dengan itu, kondisi lingkungan pemijahan berlangsung  saat puncak pasang tinggi dengan suhu rata-rata harian perairan 30,3ºC dan curah hujan yang masih tinggi di bulan Maret. Informasi ilmiah ini akan bermanfaat untuk mengembangkan riset dan tehnik reproduksi karang di alam dan laboratorium sebagai upaya merestorasi dan merepopulasi jenis karang tertentu. Kata kunci : Acropora spp, reproduksi seksual, Kepulauan Spermonde It has been thought that Indonesian marine tropical waters have less environmental variability, so that spawning period of coral extend for several months and occured during different lunar phases. Therefore the timing of coral spawning in a year cannot be predicted especially for monthly, daily and hourly scales. This study was aimed to investigate the reproductive pattern, and the environmental cues of Acropora spp. in Spermonde Islands  reefs of Makassar. Spawning corals have been determined the presence of mature gonad and spawning event in their habitats (in situ) and in the laboratory (ex situ) of Marine Station of Barrang Lompo Island, Hasanuddin University. Here we showed that seven species of Acropora spp. spawned in February and March of rainy season for consecutive three years (2010, 2011, 2012). The multispecific broadcasting spawning took place in lunar period (0?2 AFM) at 06:10?07:00 pm). The spawning time occured in high tide and the temperature was 30,3oC. This study will be useful for development of coral reproduction research and technique in both field and laboratory as an effort to restore coral reef and enhance coral population in particular. Keywords:  Acropora spp, sexual reproduction, Spermonde Islands
EFFECT OF EXPOSURE TIME OF PREGNANT FEMALES GUPPIES, POECILIA RETICULATA PETERS, IN 17A-METHYLTESTOSTERONE SOLUTION ON SEX RATIO OF THEIR OFFSPRINGS Junior, M. Zairin; Yunianti, A.; Dewi, R.R.S.P.S.; Sumawidjaja, Kusman
Jurnal Akuakultur Indonesia Vol. 1 No. 1 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.593 KB) | DOI: 10.19027/jai.1.47-54

Abstract

ABSTRACTThis experiment was carried out to study the effect of female broodstocks immersion in 17amethyltestosterone (MT) solution on sex ratio of their offspring, Three-months old males and females were paired to mate in aquaria for four days.  Broodstocks were fed with frozen blood worm and water flea 2-3 tii-nes daily.  Twelve days after mating, female broodstock were treated by immersing in 2 mg/1 MT solution for 0. 6, 12, 24 and 48 hours.  Birthed guppy babies were collected and reared separately from their parents.  During the rearing period, the babies were fed with artemia nauplius and silkworm until identification for male and female.  Percentage of female offspring in control group were higher than those of male.  Exposure of pregnant females to MT solution for 0, 6, 12, 24, and 48 hours resulted in 42, 1'/o, 51%, 84,6%, 1 00%, dan 100% of males offspring, respectively.  The best result was obtained from 24 hours treatment.  Longer treatment duration tend to shorten time interval between treatment and birth. Key words :  Guppy, 17(x-methyltestosterone, exposure time, broodstock immersion, sex ratio   ABSTRAK Penefitian ini bertuiuan untuk mengetahui pengaruh lama waktu perendaman induk di dalam larutan hormon 17a-metiltestosteron (MT) terhadap nisbah kelamin ikan gapi.  Induk Ikan gapi berumur tiga bulan dikawinkan berpasangan di dalam akuarium selama empat hari Induk diberi pakan cuk merah beku dan kutu air dengan frekuensi 2-3 kali sehari.  Dua belas hari setelah masa perkawinan, induk betina diberi perlakuan berupa perendaman di dalam larutan MT 2 mg/1 selama 0 (kontrol), 6, 12, 24, dan 48 jam.  Anak-anak ikan gapi yang baru lahir dipelihara terpisah dari induknya.  Selama masa pemeliharaan, anak ikan gapi diberi pakan nauplius artemia dan cacing rambut.  Pemeliharaan berlangsung sampai jenis kelamin anak ikan gapi dapat diidentifikasi.  Persentase anak ikan gapi betina pada semua ulangan kontrol lebih tinggi daripada .iantan dengan perbandingan persentase rata-rata 57,9%: 42,1%.  Adapun pada lama waktu perendaman 0, 6, 12, 24, dan 48 jam, menghasilkan persentase anak ikan gapi berfenotip jantan berturut-turut sebesar 42,1%, 51%, 84,6%, 100%, dan 100%.  Lama waktu perendaman terbaik adalah 24 jam.  Selain itu terdapat kecenderungan bahwa semakin lama waktu perendaman semakin cepat kelahirannya. Kata kunci :  Ikan gapi, 17ot-metiltestosteron, lama perendaman, perendaman induk, nisbah kelamin