Muhammad Jusuf
Department Biolog y, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University

Published : 14 Documents
Articles

Found 14 Documents
Search

HUBUNAGN KEMAMPUAN PERGANTIAN INANG DENGAN PLASTISITAS GENETIKA PADA CENDAWAN BLAS PADI (PYRICULARIA GRISEA) Listiyowati, Sri; Widyastuti, Utut; Rahayu, Gayuh; Hartana, Alex; Jusuf, Muhammad
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1504.563 KB)

Abstract

The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
SCREENING OF GENOMIC LIBRARY OF SOYBEAN CULTIVAR LUMUT BY USING PEROXIDASE GENE FROM ARABIDOPSIS THALIANA AS PROBE Suharsono, Suharsono; Juliyanto, Teguh; Jusuf, Muhammad
Jurnal ILMU DASAR Vol 11 No 1 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.269 KB)

Abstract

Screening to genomic library of soybean cv. Lumut by using gene encoding for peroxidase (per) from A. thaliana as a probe has an objective isolate the whole gene of per from soybean. The probe was labeled by non-radioisotope alkalin phosphatase. Screening was done by two steps. The first, screening was done to 105 recombinant lambda phages containing genome of soybean cv. Lumut. After southern hybridization, positive signal of plaques were isolated and screened for the second time. After second screening, some recombinant lambda phages containing putatively per genes were isolated. Excision from recombinant lambda phages into recombinant plasmid was successfully done in Escherichia coli strain BM25.8. The plasmid DNAs were isolated from E. coli strain BM25.8 and introduced into E. coli strain DH5? for multiplication. Plasmid DNAs were digested by EcoRI and transferred onto nylon membrane hybond N+. Southern hybridization analysis showed that one clone, L10/R/3/4, contain per gene in the 7.7 kb EcoRI fragment. This fragment is inserted into pSportI. 
ANALISIS GENERASI F2 DAN SELEKSI PERTAMA DARI PERSILANGAN KEDELAI ANTARA KULTIVAR SLAMET DAN WASE Suharsono, ,; Jusuf, Muhammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v37i1.1390

Abstract

The research had an objective to analyse the population of F2 and first selection (S1) generations of the cross between soybean cultivar Slamet and Wase. The ultimate goal of this research is to obtain the elite cultivars of soybean having high yield, big seeds, and tolerant to acid and aluminum stresses. The genetic variance and heritability in the broad sense of all characters observed of F2 population were very high. The seed productivity of F2 population was higher than that of Slamet and Wase cultivars. The size of seeds of F2 population was bigger than that of Slamet and comparable to that of Wase. The heritability in the broad sense of all characters of F2 population was very high because the maximum segregation occurs in F2 and the two parents had a very different genetic back ground. By using 7.5% selection intensity based on productivity, we got selected F2 population having productivity two times than that of Slamet. Seeds of this selected F2 population were bigger than that of F2 population and cultivar Slamet. The S1 population had seed productivity higher than Slamet and Wase cultivars. The heritability in the broad sense of all characters of S1 population was smaller than that of F2 population caused by the selection. The selection by 4.8% intensity resulted the selected S1 population having productivity three times than that of cultivar Slamet and big seeds. The selected S1 population had a phenotype variance of productivity smaller than S1 population. Therefore, the selected S1 population is very potential to be developed as elite soybean cultivars.   Key words: soybean, genetic variance, heritability, selection, F2 population
DISTRIBUTION OF Hoya multiflora Blume AT GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Rahayu, Sri; Kusmana, Cecep; Abdulhadi, Rochadi; Jusuf, Muhammad; Suharsono, Suharsono
Indonesian Journal of Forestry Research Vol 7, No 1 (2010): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hoya multiflora is one of the valuable germplasm in Indonesia that has been utilized as ornamental and medicinal plant. This epiphytic plant faces problems in decreasing habitat. As a means for developing a habitat framework for describing the distributions and ecological relations of H.multiflora at Gunung Gede Pangrango National Park, Bogor, Indonesia, ecological study of this species was established over the ranges of altitudes and characteristic vegetation structural types (primary and secondary forest, and plantation) present in the Park. Recognizing the fact that such study requires multidisciplinar y data, this paper explores the evidences from both herbarium sheets and field observations. The result of the study showed that the population of this species was only found at the Bodogol Research Station at elevation of 700 - 900 m above sea level (a.s.l.). Thus, the facts contradict with the evidence from the herbarium sheets of the Herbarium Bogoriense which have presumed that this species has a wide variation of altitudinal range from 20 to 1500 m a.s.l. (Indonesia) or 200 - 1400 m a.s.l. ( Java). The Bodogol’s population showed the clumped type of dispersion (Morisita’s Index = 1.35), which indicated such environment that was characterized by patchy resources. Direction and speed of wind coupled with the topography are ecological factors that affect to the distribution of this parachute typed seeds of the H.multiflora.
Identifikasi homolog TcAGL-15 untuk penanda embriogenesis tanaman kakao melalui pendekatan bioinformatika Identification of TcAGL-15 homolog in the embryogenic culture from the zygotic embryo explant TRIASTANTO, Oktaviany Ferry; JUSUF, Muhammad; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 74, No 2: Desember 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v74i2.102

Abstract

Summary One of the major problems encountered in micropropagation of cacao through tissue culture is very low frequency of embryo formation. Embryogenesis is believed to have key regulatory gene determining the process. Understanding such gene may help to solve problems in the regeneration process. One of the genes reported to involve in the embryogenesis is AGAMOUS-like 15 (AGL-15). This gene has an important role in the regulation of early embryogenesis in several plants. This experiment aimed to identify AGL-15 homolog in cacao through bioinformatics approach. The first step of this experiment is to identify the AGL-15 homolog using hetero-logous primers from DNA genomic isolated from leaves of cacao plants. The sequence of the AGL-15 fragment was used in designing specific primers for longer AGL-15 fragment. These primers were then used to identify AGL-15 gene using total RNA isolated from cultured zygotic embryos. Differential pattern of AGL-15 gene expression was observed in zygotic embryos cultured for five weeks. AGL-15 heterologous primers designed from several plants could be used to identify cacao AGL-15 homolog. The putative cacao AGL-15 gene could be identified from zygotic embryos. The differential pattern of the AGL-15 gene expression is a strong indication that AGL-15 can be used as an embryogenesis marker in cacao plants.Ringkasan Salah satu kendala perbanyakan kakao melalui kultur jaringan adalah sulitnya embriogenesis, yang diduga melibatkan satu atau lebih gen kunci yang menentukan proses tersebut. Keberhasilan mengidentifikasi gen-gen kunci akan membantu menyelesaikan masalah dalam regenerasi embrio kakao. Salah satu gen yang diduga terlibat dalam proses ini adalah AGAMOUS-like 15 (AGL-15). Gen ini berperan pada regulasi selama masa awal perkembangan embrio beberapa tanaman. Penelitian ini bertujuan untuk mengidentifikasi homolog AGL-15 pada kakao melalui pen-dekatan bioinformatika dan RT-PCR. Pene-litian diawali dengan identifikasi homolog AGL-15 dari DNA genomik daun kakao meng-gunakan primer heterologus. Sekuen fragmen homolog AGL-15 yang diperoleh, kemudian digunakan untuk merancang primer spesifik AGL-15 yang berukuran lebih panjang. Primer ini selanjutnya digunakan untuk meng-identifikasi gen AGL-15 dari RNA total embrio zigotik. Pengamatan pola pita gen AGL-15 dilakukan pada kultur in vitro embrio zigotik yang berumur lima minggu. Primer hetero-logus gen AGL-15 yang berasal dari berbagai tanaman, mampu mengidentifikasi keberadaan homolog  gen  tersebut  pada  tanaman   kakao. Fragmen homolog AGL-15 putatif tanaman kakao teridentifikasi pada tingkat RNA embrio. Dengan adanya pola diferensial dari ekspresi gen AGL-15 hingga lima minggu pertama perkembangan embrio, ada indikasi kuat bahwa fragmen homolog AGL-15 dapat menjadi penanda embriogenesis pada tanaman kakao.
DISTRIBUTION OF Hoya multiflora Blume AT GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Rahayu, Sri; Kusmana, Cecep; Abdulhadi, Rochadi; Jusuf, Muhammad; Suharsono, Suharsono
Indonesian Journal of Forestry Research Vol 7, No 1 (2010): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2010.7.1.42-52

Abstract

Hoya multiflora is one of the valuable germplasm in Indonesia that has been utilized as ornamental and medicinal plant. This epiphytic plant faces problems in decreasing habitat. As a means for developing a habitat framework for describing the distributions and ecological relations of H.multiflora at Gunung Gede Pangrango National Park, Bogor, Indonesia, ecological study of this species was established over the ranges of altitudes and characteristic vegetation structural types (primary and secondary forest, and plantation) present in the Park. Recognizing the fact that such study requires multidisciplinar y data, this paper explores the evidences from both herbarium sheets and field observations. The result of the study showed that the population of this species was only found at the Bodogol Research Station at elevation of 700 - 900 m above sea level (a.s.l.). Thus, the facts contradict with the evidence from the herbarium sheets of the Herbarium Bogoriense which have presumed that this species has a wide variation of altitudinal range from 20 to 1500 m a.s.l. (Indonesia) or 200 - 1400 m a.s.l. ( Java). The Bodogol’s population showed the clumped type of dispersion (Morisita’s Index = 1.35), which indicated such environment that was characterized by patchy resources. Direction and speed of wind coupled with the topography are ecological factors that affect to the distribution of this parachute typed seeds of the H.multiflora.
TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR : AKUMULASI PROLIN DAN ASAM ABSISIK DAN HUBUNGANNYA DENGAN POTENSIAL OSMOTIK DAUN DAN PENYESUAIAN OSMOTIK Sopandie, Didy; Hamim, ,; Jusuf, Muhammad; Heryani, Nani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.078 KB) | DOI: 10.24831/jai.v24i1.1616

Abstract

In this experiment. the changes on leaf osmotic potential and accumulation of proline and abscisic acid were identified from drought-tolerant and drought - sensitive soybean genotypes. Three drought - tolerant (Mlg 2805, Mlg 2984 and Mlg 2999) and two sensitive soybean genotypes (Mlg 2510 and Mlg 3541) were subjected to drought condition created by regulating water supply in greenhouse.  The results revealed that exposing plants to drought stress brought about a decrease of leaf osmotic potential. The decrease of which was greater in drought-tolerant genotypes (6.91 to 10.11 bars) than in sensitive genotypes (0.55 to 0.69 bars). The decreasing of leaf osmotic potential was followed with increasing praline accumulation, especialy for Mlg 2805. Only Mlg 2805 showed the significant ABA accumulation when the plants were subjected to drought stress. It is suggested that the drought tolerance was associated with the reduction of leaf osmotic potential (osmotic adjusment) in which proline might play an important role. The role of ABA could not be clarified since there had been a great variability in ABA content of all tolerant genotypes.
TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR: UJI LAPANG BEBERAPA GENOTIPE TOLERAN Sopandie, Didy; Hamin, ,; Jusuf, Muhammad; Supijatno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 25 No. 2 (1997): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.11 KB) | DOI: 10.24831/jai.v25i2.1609

Abstract

The purpose of this field verification was to determine the stability of yield of several drought-tolerant soybean genotypes selected from green house evaluation. The plants were planted at Muneng, Probolinggo in dry season (June-September 1995) with and without irrigation. From 5 tolerant genotypes, Mlg 2999 and Mlg 3474 gave a good stability of tolerance as evidence by less significance of growth and grain yield reduction. The tolerant genotypes of Mlg 2805 and Mlg 2984 suffered from tremendous leaves and pods abcission, leading to low yield.
KERAGAAN DAN KERAGAMAN GENETIK SIFAT-SIFAT KUANTITATIF KEDELAI (GLYCINE MAX L. MERRILL) PADA GENERASI SELEKSI F6 PERSILANGAN VARIETAS SLAMET X NAKHONSAWAN1 Jambormias, Edizon; Sutjahjo, Surjono H.; Jusuf, Muhammad; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1033.104 KB) | DOI: 10.24831/jai.v35i3.1327

Abstract

A field experiment to obtain new improved soybean varieties with higher yield and seed size was conducted by crossing Slamet Variety (high yield, small seed size) with Nakhonsawan (large seed size) in order to produce F6 selected generation. The process of selection and evaluation was conducted during a 3.5 month period, from August to December 2003 in KP IPB Sindang Barang Bogor. The pedigree selection method was used in the experiments. Data was analyzed based on information of set of total data, relatives and individually. The results showed that low performance for all traits compared to the Slamet variety except seed size and seed production traits, and higher than Nakhonsawan variety except seed size. However, genetic variances and heritabilities were high for all traits except number of branch. On the other hand, distribution of genetic variances and heritabilities on all levels of relatives were small except for the within-family F6 generations. This indicated that there was an effect of over-dominance gene action. Conclusion of analysis showed existence of two families with high seed production and seed size if compared to Slamet variety.   Key words: Soybean, selection, performance, genetic variability, and heritabilities.
DISTRIBUTION OF Hoya multiflora Blume AT GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Rahayu, Sri; Kusmana, Cecep; Abdulhadi, Rochadi; Jusuf, Muhammad; Suharsono, Suharsono
Indonesian Journal of Forestry Research Vol 7, No 1 (2010): Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2010.7.1.42-52

Abstract

Hoya multiflora is one of the valuable germplasm in Indonesia that has been utilized as ornamental and medicinal plant. This epiphytic plant faces problems in decreasing habitat. As a means for developing a habitat framework for describing the distributions and ecological relations of H.multiflora at Gunung Gede Pangrango National Park, Bogor, Indonesia, ecological study of this species was established over the ranges of altitudes and characteristic vegetation structural types (primary and secondary forest, and plantation) present in the Park. Recognizing the fact that such study requires multidisciplinar y data, this paper explores the evidences from both herbarium sheets and field observations. The result of the study showed that the population of this species was only found at the Bodogol Research Station at elevation of 700 - 900 m above sea level (a.s.l.). Thus, the facts contradict with the evidence from the herbarium sheets of the Herbarium Bogoriense which have presumed that this species has a wide variation of altitudinal range from 20 to 1500 m a.s.l. (Indonesia) or 200 - 1400 m a.s.l. ( Java). The Bodogol’s population showed the clumped type of dispersion (Morisita’s Index = 1.35), which indicated such environment that was characterized by patchy resources. Direction and speed of wind coupled with the topography are ecological factors that affect to the distribution of this parachute typed seeds of the H.multiflora.