I Made Kardena
Fakultas Kedokteran Hewan Universitas Udayana

Published : 77 Documents
Articles

SEROPREVALENSI VIRUS EGG DROP SYNDROME PADA BEBEK PETELUR DI KECAMATAN NEGARA, KABUPATEN JEMBRANA, PROVINSI BALI (SEROPREVALENCE OF EGG DROP SYNDROME VIRUS IN LAYER DUCKS IN NEGARA DISTRICT, JEMBRANA REGENCY, BALI PROVINCE) Kencana, Gusti Ayu Yuniati; Kardena, I Made; Puspitayani, Putu Mira
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.2.248

Abstract

A study was conducted to determine disease seroprevalence of egg drop syndrome (EDS) virus in layer ducks in Negara district, Jembrana, Bali. A total of 180 samples of laying duck serum with different management systems were used in this research. A number of 90 serum out of 180 samples were taken from ducks with an extensive management system (reared in the ricefield), and 90 other serum samples were taken from intensive ducks (caged) from community farms in six  villages randomly taken from Nagara districts. Serum sampel were collected from EDS un-vaccinated ducks. Serum samples were tested using an hemagglutination/inhibition test (HA/HI test). Data were analyzed using chi-quadrat analysis (X2). The results showed that duck layers maintained in n Negara district had EDS antibody titer of 23 to 29 HI units. The results showed that  68 (75.56% ) of layer ducks with an extensive management system have EDS antibody titer, whereas  only 18 (20%) of layer ducks with an intensive management system have EDS antibody titer. This indicates layer ducks in both management systems have antibody to EDS virus without EDS vaccination and that the layer ducks reared in ricefield has higher seroprevalence percentage than layer ducks reared in intensive cages.
PERBANDINGAN TINGKAT AUTOLISIS ANTARA OTOT DAN HATI SAPI BALI PADA BEBERAPA PERIODE WAKTU PENGAMATAN Putri, Kristi Agusti; Berata, I Ketut; Kardena, I Made
Indonesia Medicus Veterinus Vol 4 (5) 2015
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui perbandingan tingkat autolisis antara otot dan hati sapi bali pada beberapa periode waktu pengamatan. Spesimen diambil dari otot dan hati sapi bali yang dipotong di rumah pemotongan hewan Kota Denpasar. Spesimen yang diambil sebanyak tujuh potongan hati dan tujuh potongan otot masing-masing dari individu sapi yang berbeda-beda yang dimasukkan ke dalam tabung jaringan yang berisi Neutral buffer formalin (NBF) 10 % pada beberapa periode waktu yaitu pada jam ke-0, 2, 4, 6, 8,10, dan 12. Selanjutnya masing-masing diproses untuk pembuatan preparat histopatologi. Variabel yang diperiksa meliputi indikasi autolisis yaitu : jaringan hiperkromatik sampai hilangnya inti sel. Analisis tingkat autolisis antara otot dan hati dilakukan dengan pengamatan histopatologi jaringan di bawah mikroskop pada pembesaran 1000 kali dengan pengamatan lima lapang pandang. Data hasil penelitian berupa presentase skoring autolisis, dianalisis dengan menggunakan uji non parametrik Kruskal-Wallis. Hasil penelitian menunjukkan bahwa hati sapi bali dengan nilai rata-rata 15,70%, 22,10%, 29,10%, 55,20%, 74,10%,93% dan 96,45% memiliki tingkat autolisis lebih cepat dibanding otot dengan nilai rata-rata 10%, 14,90%, 19%, 27,90%, 53%, 61% dan 83,70% . Hal ini mungkin terjadi karena hati memiliki jumlah enzim dan pembuluh darah yang lebih banyak dibandingkan otot. Perlu penelitian lebih lanjut tentang tingkat autolisis antara otot dan hati sapi bali yang berasal dari hewan sakit atau mati akibat penyakit infeksius
PAPARAN FORMALIN MENGHAMBAT PROSES SPERMATOGENESIS PADA MENCIT Sri Surya Heryani, Luh Gde; Werdi Susari, Ni Nyoman; Kardena, I Made; Dewi Indira Laksmi, Desak Nyoman
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Formaldehyde is one of the exogenous free radicals. During formaldehyde exposure, there will be morefree radical or reactive oxygen compound formed through electron transport chain. Excessive reactiveoxygen compound triggers the process of lipid peroxide reaction in the membrane of spermatozoa cell. Theaims of this study were to find out that formaldehyde exposure caused abnormalities in the number of thenumber of spermatogonium type A, spermatogonium type Pachytene, 7th spermatid and 16th spermatid inspermatogenesis process.This study was experimentally and randomly pretested-posttested- with controlgroup design. The samples of this study were adult male mice strain Balb-C (age 2-3 months) with thefollowing criteria: body weight between 22-25 grams and healthy. Randomly, 48 mice were divided intothree groups, were control group, the first treatment group and the second treatment group. Prior to thetreatment, were taken a half from each group for the pre-test, by preparing microscopic preparation testicleand examination was performed to the total spermatogenic cells. The rests of the mice were used as posttestexamination after 35 days treatment. On the 36th day, all the rest mice were necropsied for microscopictesticle preparation. The result of this study showed that the formaldehyde exposure caused significantlydecrease in the number of spermatogenic cells (p<0,05), the average of type A spermatogonium cells atcontrol group, 1st treatment group and 2nd treatment group were 39,90±0,51; 20,42±0,72; 15,65±0,88respectively; spermatogonium type Pachytene were 48,47±1,28; 32,60±3,06; 23,14±3,16 respectively; 7thspermatid were 97,47±5,28; 39,98±4,28; 30,36±2,96 respectively and16th spermatid were 73,08±4,05;21,70±1,70; 16,38±1 respectively.It can be concluded that the formaldehyde exposure decreased the amountof spermatogenic cells. The result of this study is expected to be used as the baseline for further study inorder to measure formaldehyde content as a free radical in testicle .
COCONUT WATER BASED EXTENDER EFFECTS ON MOTILITY,VIABILITY, AND DNA INTEGRITY OF CHILLED KINTAMANI DOG SEMEN Gunawan, I Wayan Nico Fajar; Kardena, I Made; Suatha, I Ketut; Puja, I Ketut
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
PCS-7 LEAD DETECTION IN BLOOD AND LIVER OF CATTLE SOLD IN THE TRADITIONAL MARKET OF DENPASAR CITY Berata, I Ketut; Susari, Ni Nyoman Werdi; Sudira, I Wayan; Kardena, I Made
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Lead contamination in many foods is reported to endanger consumers' health. Beef as one of the food of animal origin can be contaminated by lead mainly due to the maintenance of cattle in contaminated areas. Balinese cattle that are kept in urban landfills are reported to be contaminated with high levels of lead in the blood and in other tissues with lower levels [4]. The threat of human health from eating foods that contain leads can cause anemia because leads can substitute iron in hemoglobin [2]. Lead poisoning can also cause malfunction of various organs such as liver, kidney [1], lungs, spleen [7] and brain [9] in the form of cognitive impairment [5]. The high threat due to contamination of leads to health, then the meat health check should also be made on the presence of leads. One of the inspection efforts on the presence of lead contamination in beef, then the place of sale in the traditional market is a good location for sampling examination.
CEMARAN TIMAH HITAM DALAM DARAH SAPI BALI YANG DIPELIHARA DI TEMPAT PEMBUANGAN AKHIR KOTA DENPASAR (BLOOD LEAD CONTAMINATION IN BALI CATTLE REARED IN THE AREA OF FINAL DISPOSAL OF DENPASAR) Berata, I Ketut; Susari, Ni Nyoman Werdi; Kardena, I Made; Ariana, I Nyoman Tirta
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle are known for having high adaptability to any environmental condition and have proven tosurvive in critical environments including landfills. The aim of this study was to determine the relationshipbetween Serum Glutamic Pyruvic Transaminase /Serum Glutamic Oxaloacetic Transaminase (SGPT/SGOT) levels and lead (Pb) level in blood of bali cattle reared in the landfill Denpasar. A total of 22Balicattle were used in this study was. The blood was drawn from the jugular vein and 10 ml blood werecollected using tubes containing EDTA 0.5% and 10 mL without EDTA tubes. Levels of SGPT and SGOTwere measured by using Auto analyzer (Refloton (R) plus) method, while the measurement of Pb in bloodplasma was conducted by using atomic absorption spectrophotometry (AAS) method. Result of the SGPTand SGOT measurement obtained were relatively varied with an average of SGPT = 29.96±5.67 U/L andSGOT = 78.82±17.62.U/L. Similarly, Pb measurement results vary widely between 4.004 to 10.216 withan average of 6.595±1.85 ppm. Correlation analysis showed that there was an association between SGOTwith Pb levels. However, no correlation was observed between the SGPT with Pblevels. These resultsindicate that the levels of lead may cause pathological tissue cells in organs other than liver. Its concludedthat heavy metalPb is found in the blood of bali cattle reared in Denpasar city landfill, and it may becorrelated with the level of the SGOT and may cause pathological tissue cells organs.
PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN ASHITABA TERHADAP HISTOPATOLOGI LAMBUNG MENCIT JANTAN (THE EFFECT OF ETHANOL EXTRACT OF ASHITABA LEAVES TOWARD THE GASTRIC HISTOPATHOLOGY OF MALE MICE) Wiralaga, I Putu; Sudira, I Wayan; Kardena, I Made; Darmayudha, Anak Agung Gde Oka
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study was intended to find out the effect of ethanol extract of Ashitaba leaves (Angelicakeiskei) toward the gastric histopathology of male mice. After being adapted for one week, 25 malemice (Mus musculus) were divided randomly to be five treatments. The first treatment was not given ethanol extract of Ashitaba leaves as control. The second till fourth treatment was given eachdose of 125; 250; 500; dan 1,000 mg/kg bb during 21 days orally. Necropsy for gastric organharvesting was conducted on the day of 22. The result of giving ethanol extract of Ashitaba leaves(Angelica keiskei) with a dose of 125 mg/kg bb showed that three mice undergo degeneration andinfiltration of inflammatory cells and one mice undergo necrosis. The dosing of 250 mg/kg bbshowed that three mice undergo degeneration and two mice undergo infiltration of inflammatorycells and necrosis as well. The dosing of 500 mg/kg bb showed that three mice undergodegeneration, infiltration of inflammatory cells, and necrosis. The dosing of 1,000 mg/kg bbshowed that three mice undergo degeneration and necrosis, and four mice undergo infiltration ofinflammatory cells. Based on the results of the study, It was concluded that the ethanol extract ofleaves Ashitaba dose of 125 mg / kg bw up to 1,000 mg / kg bw for 21 days did not cause structural disorders of the stomach organ histopathology of mice (Mus musculus) male. 
SYNDROMIC SURVEILLANCE: A POTENTIAL METHOD FOR ALTERNATIVE ANIMAL HEALTH SURVEILLANCE SYSTEM Kardena, I Made
Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Syndromic surveillance is an alternative surveillance system which can potentially detectanimal disease outbreaks in the early stages. Although syndromic surveillance haslimitations for the detection of sub-clinical and specific diseases, the method has the abilityto detect disease outbreaks rapidly including clinical emerging issues. In addition,syndromic surveillance is a suitable surveillance tool that can be applied in developingcountries since it is a low-cost surveillance method and is an option for poorly-resourcedveterinary services.
PENINGKATAN PENDAPATAN PETERNAK MELALUI PERBAIKAN MANAJEMEN PETERNAKAN BABI Agustina, Kadek Karang; Wirata, I Wayan; Dharmayudha, Anak Agung Gde Oka; Kardena, I Made; Dharmawan, Nyoman Sadra
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk meningkatkan pendapatan keluarga peternak babi dengan melakukan rekonstruksi dan perbaikan pada sistem peternakan babi yang diterapkan. Metode yang diterapkan pada penelitian ini adalah aplikasi dari teknik pemecahan masalah melalui kegiatan IPTEKDALIPI. Data yang dikumpulkan adalah total biaya produksi yang dihabiskan selama satu kali periode produksi yaitu sebelum dan sesudah dilakukan intervensi. Hasil menunjukkan bahwa terjadi peningkatan yang signifikan pada penghasilan peternak babi dari 31,4% menjadi 38,77%. Sehingga dapat disimpulkan bahwa penghasilan peternak babi dapat ditingkatkan melalui perbaikan sistem manajemen peternakan babi.
PREVALENSI DERMATITIS ULSERATIF PADA TUKIK LEKANG YANG DIPELIHARA DI TURTLE CONSERVATION AND EDUCATION CENTRE SERANGAN Wijaya, Annabella Ruth; Adnyana, Ida Bagus Windia; Kardena, I Made
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2018.v10.i01.p09

Abstract

Dermatitis ulseratif adalah penyakit yang paling sering menjangkiti penyu dan/atau tukik yang dipelihara di kolam-kolam penampungan, termasuk di Turtle Conservation and Education Centre (TCEC), Serangan. Penelitian dilakukan untuk mengetahui prevalensi, gambar patologi, dan lokasi jejas dermatitis ulseratif pada tukik lekang yang dipelihara di TCEC Serangan, serta perbedaan indeks kondisi tubuh tukik lekang penderita dermatitis ulseratif dengan tukik yang sehat. Prevalensi diketahui dengan menghitung jumlah tukik lekang penderita yang dibandingkan dengan total tukik di TCEC. Morfometri (straight carapace length, straight carapace width, curved carapace length, curved carapace width) dan berat badan tukik diukur untuk mengetahui indeks kondisi tubuh tukik lalu perbedaan indeks kondisi tubuh dibandingkan dengan Uji - T tidak berpasangan. Sampel jaringan kulit diproses menjadi preparat dan diwarnai dengan pewarnaan rutin Hematoxylin eosin(HE). Hasil penelitian ini yaitu : prevalensi dermatitis ulseratif pada tukik lekang di TCEC adalah 16,2%, ditemukan jejas luka dengan kerak kekuningan berdiameter 2 mm hingga 2 cm dan secara mikroskopis ditemukan infiltrasi heterofil dan sel mononuklear pada dermis kulit yang disertai dengan erosi, hiperkeratosis parakeratosis, dan materi nekrosis yang berisi debris sel. Jejas paling sering ditemukan pada area leher (63,04%), diikuti dengan flippers, kepala, kulit dekat karapas, kelopak mata, dan area leher dan flippers secara bersamaan. Terdapat perbedaan indeks kondisi tubuh yang sangat nyata antara tukik lekang penderita dengan yang sehat, dimana rerata indeks kondisi tubuh tukik penderita lebih besar dari yang sehat. Perlu dilakukan perbaikan manajemen pemeliharaan tukik di TCEC.
Co-Authors AAA Mirah Adi, AAA Mirah Abdul Azis Nasution Abriansyah, Mohammad Ghaiz Aida Louise Tenden Rompis Anak Agung Ayu Mirah Adi Anak agung ayu mirah adi Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Andari, Claudia Murti Anggreni, Ni Kadek Wiwik Arhiono, Haru Nira Putra Asih, Ni Putu Trisna Bayu Setiabudi, Bayu Berata, I Ketur Boedi P. Yenniastuti, Boedi P. Calvin Iffandi Chanda Preanger, Chanda de Carvalho, Rui Daniel Depari, Eldarya Envisari Desak Nyoman Dewi Indira Laksmi Desi Elrini Sarah Alunat Deska Gilang Mustika, Deska Gilang Dewa Ayu Widia Kusuma Ningrat, Dewa Ayu Widia Kusuma Edward Emanuel Mango, Edward Emanuel Elyda ., Elyda Endris Arif Wicaksono, Endris Arif Farhan Abdul Hasan, Farhan Abdul Farhani, Annas Ferbian M. Siswanto, Ferbian M. Ferbian Milas Siswanto Findri Andriani, Findri Fitri Irawan Rahmawandani Gunawan, I Made Dodi Gunawan, I Wayan Nico Fajar Gusti Ayu Yuniati Kencana Hendrina Konda M Meha, Hendrina Konda M Herbert . Hidayatullah, Lalu Syarif I Dewa Gde Tara Damayanthi, I Dewa Gde Tara I Dewa Made Adhiwitana, I Dewa Made I Gusti Agung Arta Putra, I Gusti Agung Arta I Gusti Agung Gde Putra Pemayun, I Gusti Agung Gde Putra I Gusti Ayu Agung Suartini I Gusti Ketut Suarjana I Gusti Made Krisna Erawan, I Gusti Made Krisna I Gusti Ngurah Kade Mahardika I Ketut Berata I Ketut Eli Supartika I Ketut Gunata I Ketut Puja I Ketut Suatha I Made Damriyasa I Made Dwinata I Made Merdana I Made Sukada I Nengah Kerta Besung I Nyoman Suarsana I Nyoman Suartha I Nyoman Tirta Ariana I Putu Wiralaga, I Putu I Wayan Batan I Wayan Gorda I Wayan Sudira I Wayan Wirata IB Oka Winaya Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana imam sobari Indahyani, Ni Made Dwi Indrawan, Hieronimus Irhas, Rajiman Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Kadek Karang Agustina Kartika, Erena Hajar Kencana, Gusti Ayu Yunianti Ketut Budiasa Ketut ELI Supartika Ketut Tono Pasek Gelgel Komang Ariya Hendrayana Kristi Agusti Putri, Kristi Agusti Lagho, Engelbertus Efraim Luh Gde Sri Surya Heryani Luh Made Sudimartini Mahyuzar Mahyuzar Maria, Noviriolla Muhamad Furkam Fadilah, Muhamad Furkam Ni Ketut Suwiti Ni Luh Eka Setiasih NI LUH WATINIASIH Ni Made Rina Yulinta Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Wayan Listyawati Palgunadi Nugraha, Elisabeth Yulia Nur Hanifah Septiani, Nur Hanifah Nurrohman, Fahmi Galuh Nurul Faiziah Nyoman Mantik Astawa Nyoman Sadra Dharmawan Paranitha, Dewa Ayu Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Prabandari, Anak Agung Istri Vera Pranatha, Wahid Danang PRANSIKA EKSY YONITA Prasetyo, Yoga Eka Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Puspitayani, Putu Mira Putra, Wahyu Semadi Putri Udayani Putri, Raden Roro Chandra Gita Soetarmono Putu Dede Yudi Utama, Putu Dede Yudi Rasdiyanah . Ratih, Dwi Kusuma Komala Sakura, Yoakim Wilson Samsuri Samsuri Septianingsih, Rayni Shantika Pratistha, Ni Wayan Apsari Silaban, Jesiaman Sri Kayati Widyastuti Sri Milfa STEVEN YOHANES BOGIA TJOKORDA ISTRI AGUNG Cintya Dalem Tri A. Putra, Tri A. Tri Adi Putra, Tri Adi Wicaksana, I Gede Hendra Prasetya Widiarta, Lutfi Widyastuti, Ni Wayan Helpina Wijaya, Annabella Ruth Yesi Veronica Sitepu Yuliantari, Ida Ayu Made Yunita Lestyorini