L.B.S. Kardono
Research Center for Chemistry, Indonesian Institute of Sciences Puspiptek Area, Serpong 15314 and Pharmacy Graduate School, University of Pancasila, Jagakarsa, Jakarta 126410, Indonesia

Published : 3 Documents
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Isolation of Bioactive Compounds from Aspergillus terreus LS07 Dewi, Rizna Triana; Tachibana, Sanro; Dewi, Puspa; Kardono, L.B.S.; Ilyas, Muhammad
Indonesian Journal of Chemistry Vol 14, No 3 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.866 KB) | DOI: 10.22146/ijc.21243

Abstract

This study aims to search for the active components from Aspergillus terreus LS07 which isolated from an Indonesian soil. Bioassay-guided fractionations of the ethyl acetate (EtOAc) extract against α-glucosidase and DPPH free radical to give four isolated compounds: oleic acid (1), ergosterol (2), butyrolactone I (3), and butyrolactone II (4). The structures of these metabolites were assigned on the basis of detailed spectroscopic analysis. Oleic acid (1) was showed significant activity toward α-glucosidase with IC50 value of 8.54 μM, but not for antioxidant. Butyrolactone I (3) and II (4) were showed significant activities against the α-glucosidase with their IC50 values at 52.17 and 96.01 μM, and those against DPPH free radicals at 51.39 and 17.64 μM, respectively. On the other hand, ergosterol (2) did not show any activities.
INFLUENCE OF TAPIOCA FLOUR CONCENTRATION AND LEGUMES VARIETIES ON CHARACTERISTIC OF BLACK ONCOM WITH Mucor sp AS INOCULUM Iskandar, Yetti Mulyati; Priatni, Sri; Kardono, L.B.S.
Teknologi Indonesia Vol 33, No 1 (2010)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v33i1.17

Abstract

Oncom is a product prepared by fermenting cooked peanut presscake as substrate mixed with tapioca fl our using Mucor sp as inoculum. The aim of this research is to determine the chemical composition and nutritional contents of black oncom using lupine (Lupinus angustifolius) and pigeon pea (Cajanus cajan) as substrate instead peanuts ( Arachis hypogaea ) to evaluate its potential as a new source of protein. The moisture content, pH, texture, solidness, hardness, mold growth and colour of black oncom fermented product were observed. Various tapioca fl our concentration in amount of 5%, 20% and 35% respectively were mixed on peanuts, lupine and pigeon pea as substrates. The fermentation process was conducted for 48 hours at 30oC.The result revealed that the addition 20% of tapioca to substrate showed the best performance of black oncom product and had good sensory characteristics such as texture, taste, aroma and colour based on the sensory evaluation test.
Antioxidant Compound from The Rhizomes of Kaempferia Angustifolia Collected from Purworejo, Central Java Secondary Forest Kardono, L.B.S.; Minarti, Minarti; Sutaryo, B.; Kawanishi, K.
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 1 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.071 KB)

Abstract

Kaempferia angustifolia Rosc. (Zingiberaceae) dan K. rotunda L. di Jawa dikenal sebugai Kunci pepet dan Kunir putih. Kedua macam tumbuhan tersebut biasanya tumbuh di daerah hutan sekunder di Jawa. Rhizoma dari K. angustifolia berbentuk serbuk kering atau tunbuhan segar biasa dijual di pasar tradisional di Indonesia, dan dikenal sebagai pencegah dan pengobatan kanker secara tradisional. Ekstrak khloroform dari rhyzoma K. Angustifolia menunjukkan efek antioksidan (scavenging) yang signifikan terhadap radikal bebas 1,7-difenil2 pikril hidrasil (DPPH). (IC50 = 370 ug/mL). Dua macam senyawa dari ekstrak khloroforin telah diisolasi dan diidentifikasi. Senyawa 1, suatu khalkon yang telah dikenal, yaitu, 2'hidroksi-4', 4', 6'-trimetoksi khalkon adalah senyawa yang aktif (IC5, = 97 ug/mL). Senyuwu 2, suatu sikloheksan epoksid yang telah dikenal, (+)-krotepoksida, tidak aktif (IC;, = 690 ugi mL) Struktur senyawa 1 dan 2 diidentifikasi berdasarkan data spektroskopik, MS dan 2DNMR (HMQC dan HMBC) dan dibandingkan dengan data yang telah dipublikasikan.