Gusti Ayu Yuniati Kencana
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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SEROPREVALENSI VIRUS EGG DROP SYNDROME PADA BEBEK PETELUR DI KECAMATAN NEGARA, KABUPATEN JEMBRANA, PROVINSI BALI (SEROPREVALENCE OF EGG DROP SYNDROME VIRUS IN LAYER DUCKS IN NEGARA DISTRICT, JEMBRANA REGENCY, BALI PROVINCE) Kencana, Gusti Ayu Yuniati; Kardena, I Made; Puspitayani, Putu Mira
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.2.248

Abstract

A study was conducted to determine disease seroprevalence of egg drop syndrome (EDS) virus in layer ducks in Negara district, Jembrana, Bali. A total of 180 samples of laying duck serum with different management systems were used in this research. A number of 90 serum out of 180 samples were taken from ducks with an extensive management system (reared in the ricefield), and 90 other serum samples were taken from intensive ducks (caged) from community farms in six  villages randomly taken from Nagara districts. Serum sampel were collected from EDS un-vaccinated ducks. Serum samples were tested using an hemagglutination/inhibition test (HA/HI test). Data were analyzed using chi-quadrat analysis (X2). The results showed that duck layers maintained in n Negara district had EDS antibody titer of 23 to 29 HI units. The results showed that  68 (75.56% ) of layer ducks with an extensive management system have EDS antibody titer, whereas  only 18 (20%) of layer ducks with an intensive management system have EDS antibody titer. This indicates layer ducks in both management systems have antibody to EDS virus without EDS vaccination and that the layer ducks reared in ricefield has higher seroprevalence percentage than layer ducks reared in intensive cages.
RESPONS ANTIBODI SEKUNDER TERHADAP PENYAKIT TETELO PADA AYAM PETELUR PASCAVAKSINASI ULANGAN DENGAN VAKSIN TETELO AKTIF (NEWCASTLE DISEASESECONDARY ANTIBODY RESPONSE AFTER REVACCINATION IN LAYER WITH THE ACTIVE ND VACCINE) Kurnianto, Andika Budi; Kencana, Gusti Ayu Yuniati; Astawa, I Nyoman Mantik
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Revaccination is required in order to preventNewcastle Disease (ND) reccurence inlayers chickens. Oneof vaccine for ND revaccination is freeze-died ND active vaccine containing e? 106,5EID50. Revaccinationisdone to trigger a faster secondary antibody responses in layers and can achieve protective antibody titersagainst ND that can be monitored by a hemagglutinationinhibition (HI). The aim of this study was todetermine the ND secondary antibody responses in layers after revaccination with ND active vaccine.Antibody titer of 20 layers chickens of 20 week old were determined before revaccinations (week 0) andafter revaccinations (week 1 until week 9). The first vaccination was conducted using ND-IB (NewcastleDisease-Infectious Bronchitis) at the age of 2 days through eye drops and subcutaneous injection at the ageof 5 days using a dose of 1 ampoule.Vaccination is repeated at the age of 20 weeks at a dose of 1 ½ ampoule through drinking water. Blood samples were collected on the wing vein (venous cutane ulnar) and left for 5-10 minutes at room temperature.Sera were then collected and stored at -20oC until use. HI antibody titerwas determined by micro titeration system. The HI mean titers were analyzed by Duncan test. The studyresults showed that antibody titer before revaccination was3,47 HI log 2 and the HI titers after revaccinationwere 4,02; 5,22; 6,52; 7,85; 8,4; 8,6; 7,7; 5,92; dan 3,87 HI log 2 respectivelly at weeks 1, 2, 3, 4, 5, 6, 7, 8, and9.The NDV revaccination with ND active vaccine significantly (P <0.01) increased in antibody titer inlayers starting from week 1 to week 6, but decreased following week 7 to week-9. It can be concluded thatrevaccinantion with ND active vaccine increases the antibody titers in layer chickens.
KEPEKAAN TELUR SPESIFIC PATHOGEN FREE DAN CLEAN EGG TERHADAP VIRUS FLU BURUNG (SENSITIVITY OF SPESIFIC PATHOGEN FREE EGGS AND CLEAN EGG TO THE AVIAN INFLUENZA VIRUSES SUBTYPE H5N1) Kencana, Gusti Ayu Yuniati; Suartha, I Nyoman; Nurhandayani, Arini; Ramadhan, Muh
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza which is  in Indonesia  known as Flu Burung  is caused by the avian influenza virussubtype H5N1 (AIV-H5N1). Vaccination is one of the major strategies for preventing and eradicatingAIV-H5N1 in Indonesia. Several factors can affect the potential vaccine such as viral content and mediaused for the propagation of the virus. One of the media commonly used to propagate  the virus is pathogenspecific free (SPF) embryonated chicken eggs. However, as the SPF eggs production is limited and expensive,the use of clean embryonated chicken eggs as an alternative need to examined. This study aimed todetermine the sensitivity of SPF and clean embryonated chicken eggs to the AIV-H5N1. The virus usedwas seed avian influenza virus (A/ Chicken/West Java (Subang)/29/2007)  which haa previously werepropagated  in SPF eggs and the Clean Eggs. The virus titer was determined as Embryo infective Dose 50%(EID50) using Reed and Muench method. Sensitivity of SPF eggs and Clean Egg to the VAI-H5N1 wascompared using  Chi-square statistical analysis. The titers of Avian Influenza Virus subtype H5N1 were106.83EID50/0.1ml in SPF eggs and 106.17EID50/0.1 ml in the Clean Eggs. Statistical analysis showed that,the sensitivity of SPF Eggs and Egg Clean  for the propagation of the VAI-H5N1 was not significantlydifferent.
SEROPREVALENSI PENYAKIT EGG DROP SYNDROME PADA ITIK DI DESA TUMBAK BAYUH, KECAMATAN MENGWI, BADUNG, BALI Kencana, Gusti Ayu Yuniati; Kardena, I Made; Shantika Pratistha, Ni Wayan Apsari
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.876 KB) | DOI: 10.22146/jsv.42971

Abstract

Egg Drop Syndrome can cause detrimental impacts on breeders due to reducing production and quality of theaffected eggs.This study aim was to determine the seroprevalece ofantibody against Egg Drop Syndrome (EDS) virus in ducks. Antibody titers examination was done from 75blood samplesof ducks thathave not been vaccinatedagainstEDS virus. The duck samples were collected from Tumbak Bayuh Village, Mengwi, Badung. Serological examination was held at the Virology Laboratory,Faculty of Veterinary Medicine, Udayana University by usingHaemagglutination Inhibition (HI) test. HI test results showed that 24 samples were positive contained antibody of the EDS,while 51 samples were negative. Range of EDS antibody titer observed was from24 to 27 HI units.  This results indicates that the ducks have protective antibody titer against EDS virus. The positive serum samples were also tested using HI test against Newcastle Disease (ND) virus with negative result. It can be concluded that the ducks in Tumbak Bayuh Village, Mengwi, Badung have 32% of antibody titer against EDS virus whichmight result from being exposed by EDS virus naturally.
SEROPREVALENSI PENYAKIT TETELO PADA PETERNAKAN ITIK DAN PASAR GALIRAN DI KABUPATEN KLUNGKUNG, BALI (NEWCASTLE DISEASE SEROPREVALENCE IN LIVESTOCK DUCK AND MARKETS GALIRAN OF KLUNGKUNG RECIDENCE, BALI) Yuliana, I Komang Wahyu; Kencana, Gusti Ayu Yuniati; Suartha, I Nyoman
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Ducks are one of the birds that play a role in the spread and transmission of Newcastle disease virus.Newcastle disease infection in ducks are rarely accompanied by clinical symptoms, so the ducks couldpotentially spread the disease to other birds. In general, the distribution of ducks is going from farm toMarket Poultry and vice versa. The aim of this study was to determine the seroprevalence Newcastledisease infection in ducks reared on farms and sold at market Galiran Klungkung regency. The sampleused in this study was serum unvaccinated ducks ND. The samples used were 420 samples taken from thefarm and from Klungkung Galiran Market from March to August 2012.Serum samples were tested for thepresence of NDV antibody by using hemagglutinationtest(Haemaglutination Inhibition Test / HI test) atthe Biomedical Laboratory of the Faculty of Veterinary Medicine Udayana University. The results showedthat the seroprevalence Newcastle disease virus in ducks in Galiran Market were 33.3% and in farmsamounted to 46.2%. There are differences in seroprevalence Newcastle disease (p <0.05) in March, June,and August. Overall seroprevalence in Galiran Market and Newcastle disease in livestock in Klungkungregency equal to 39.8%. It can be concluded that Newcastle disease seroprevalence in Klungkung regency isquite high and might be potentially transmit the virus to other poultry. Therefore, periodic monitoring isnecessary as an effort to early prevention.
PENYEBARAN VIRUS VAKSIN ND PADA SEKELOMPOK AYAM PEDAGING YANG TIDAK DIVAKSINASI DAN DIPELIHARA BERSAMA AYAM YANG DIVAKSINASI Kencana, Gusti Ayu Yuniati; Astawa, Nyoman Mantik; Mahardika, I Gusti Ngurah Kade; Gorda, I Wayan
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Telah dilakukan penelitian untuk mengetahui daya sebar vaksin ND aktif galurlentogenik (La Sota) dan respons immune ayam yang tidak divaksin yang dipeliharabersama ayam yang divaksin secara intramuskuler. Penelitian ini menggunakan rancanganacak lengkap pola berjenjang (split time) dengan faktor utama perlakukan vaksinasi (TO:0% divaksin dan 100% tidak divaksin , T1: divaksin 50 % dan 50 tidak divaksin dan T2:divaksin 75% dan 25% tidak divaksin) dengan sembilan kali ulangan. Faktor tambahanadalah waktu pengambilan serum (minggu ke-0, ke-1, ke-2 dan ke-3) sehingga jumlahsampel adalah 3x9x4= 108 sampel serum. Ayam umur 3 hari divaksinasi ND secara tetesmata kemudian dilakukan vaksinasi intramuskuler pada umur 21 hari sesuai perlakuan.Titer antibodi ND pada ayam perlakuan diuji dengan uji hambatanhemaglutinasi/hemagglutination inhibition (HI) satu hari sebelum vaksinasi, serta satuminggu, dua minggu, dan tiga minggu setelah vaksinasi. Data tentang titer antibodi (GMTHI)terhadap ND ditransformasi dengan akar X+1, dianalisis dengan sidik ragam dandilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa titerantibodi terhadap ND pada ayam yang tidak divaksin dipengaruhi oleh persentase ayamyang divaksin. Antibodi HI unit terhadap virus ND pada ayam yang tidak divaksinasimulai teramati pada minggu ke-2 dan ke-3 setelah vaksinasi. Titer antibodi ayam yangtidak divaksinasi pada kelompok ayam yang hanya divaksin 75% mempunyai titer antibodiyang nyata lebih tinggi dibandingkan dengan kelompok ayam yang divaksin 50% dankontrol (P<0,05). Pada kelompok ayam yang divaksin 50%, titer antibody ND pada ayamyang tidak divaksin secara statistik berbeda tidak nyata dibandingkan dengan kelompokyang divaksin 0% (P>0,05). Pada minggu ke tiga, titer antibody ND ayam yang tidakdivaksinasi pada kelompok ayam yang divaksin 75% nyata lebih tinggi dibandingkandengan pada kelompok ayam yang divaksin 50% (P,0,05). Vaksin ND aktif lentogeik LaSota dapat menyebar dari ayam yang divaksin secara intramuskuler kea yam yang tidakdivaksin
RESPON IMUN PRIMER AYAM PETELUR PASCA VAKSINASI EGG DROP SYNDROME Kencana, Gusti Ayu Yuniati; Suartha, I Nyoman; Wibawa, I Putu Wira Adi
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Penelitian ini dilakukan untuk mengetahui respon imun primer ayam petelur pasca vaksinasi Egg drop syndrome.  Sampel penelitian adalah ayam petelur jenis Isa Brown yang dipelihara di Desa Tiga, Kabupaten Bangli, Provinsi Bali. Sebanyak 25 ekor ayam petelur umur 14 minggu divaksin dengan vaksin EDS inaktif polivalen (mengandung antigen virus Newcastle disease, infectious bronchitis dan egg drop syndrome). Vaksinasi dilakukan secara intramuskuler pada otot dada. Pemeriksaan titer antibodi EDS dengan uji serologi Hambatan Hemaglutinasi (HI). Titer antibodi egg drop syndrome diperiksa sebanyak  empat kali yaitu satu kali sebelum vaksinasi dan tiga kali pasca vaksinasi. Rataan titer antibodi setiap minggu dianalisis menggunakan sidik ragam dilanjutkan dengan uji beda nyata terkecil, dan analisis regresi. Hasil penelitian menunjukkan bahwa terjadi peningkatan titer antibodi yang signifikan setiap minggunya pada ayam petelur pasca divaksinasi EDS. Titer antibodi satu minggu pasca vaksinasi sebesar 22,6 HI unit, pada dua minggu pasca vaksinasi sebesar 25,04 HI unit, dan  tiga minggu  pasca vaksinasi sebesar 26,4 HI unit.
SEROPREVALENSI PENYAKIT AVIAN INFLUENZA PADA ITIK DI KABUPATEN KLUNGKUNG Damanik, Estry Gusnita; Kencana, Gusti Ayu Yuniati; Mahardika, I Gusti Ngurah Kade
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Itik memiliki peran penting dalam penyebaran virus Avian Influenza subtipe  H5N1 karena merupakan reservoir alami virus dan infeksinya bersifat subklinis. Pendistribusian itik terjadi dari pasar unggas ke peternakan itik atau sebaliknya. Penelitian ini bertujuan untuk mengetahui perbedaan tingkat seroprevalensi virus Avian Influenza di Pasar Unggas Galiran dan peternakan itik di kabupaten  Klungkung pada saat yang bersamaan.  Sampel penelitian adalah serum dari itik yang tidak divaksin yang diambil dari pasar dan peternakan di kabupaten Klungkung.  Pengambilan  sampel dilakukan setiap bulan mulai bulan Maret sampai dengan bulan Agustus 2012.  Sampel serum selanjutnya  diuji dengan uji Hambatan Hemaglutinasi. Hasil penelitian menunjukkan seroprevalensi AI di Pasar Unggas Galiran dan peternakan itik di Kabupaten Klungkung adalah sebesar 81.4%,  perbedaan yang signifikan terjadi pada Juni dan Agustus tetapi tidak signifikan pada bulan Maret, April, Mei, dan Juli.  Seroprevalensi virus AI di Pasar Unggas Galiran adalah sebesar 76.2% dan di peternakan sebesar 86.7% dan secara statistik berbeda sangat nyata. Monitoring terhadap virus Avian Influenza berkelanjutan perlu dilakukan baik di peternakan maupun  di pasar unggas di Klungkung.
ASSESSMENT OF VIRAL CONTENT IN NEWCASTLE DISEASE VACCINE OBTAINED FROM TWO DIFFERENT POULTRY SHOPS USING PRIMARY CHICKEN FIBROBLAST CELL CULTURE Kencana, Gusti Ayu Yuniati
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Newcastle Disease (ND) is still endemic in Indonesia, characterized by its your-round occurrence. Many measures have been adopted by the government to prevent the spread of the disease including vaccination using both active and inactive vaccine. The quality of vaccine is influenced by its viral content which determines the success of ND vaccination in chicken flock. The viral content in ND vaccine can be determined by measuring its eggs lethal dose-50 (ELD50) or Tissue Culture Infective Dose-50 (TCID50) and the minimum viral content considered to be appropriate for active ND vaccine is 66,5/single dose. This study was conducted to find out the viral content of ND vaccine marketed in some poultry shops. ND vaccine of  LaSota strain were obtained from 2 different poultry shops and the viral content was determined in Chicken embryo fibroblast (CEF). The vaccines were reconstituted vaccine diluent and diluted serially in 10-fold diluted. Each dilution was inoculated into 4 wells of confluent CEF cultured in 96 well microplate. The TCID50 was then calculated by Reed and Muench method. The TCID50 of each vaccine was determined 4 times (4 replications). The result showed that the titer of the virus in the vaccine were 66,7and 67 TCID50/per dose which mean that both vaccines were still above the minimum standard of viral content recommended by some workers.
PERAN CODING DAN NON-CODING REGION DARI GEN POLIMERASE KOMPLEKS DALAM ADAPTASI VIRUS AVIAN INFLUENZA Kencana, Gusti Ayu Yuniati
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Avian influenza (AI) is an avian diseases that can cause deadly humaninfection. The disease is caused by Orthomyxoviridae virus, genus influenzavirus type A,subtype H5N1. Pathogenicity of AI virus is polygenic, which means AI virus isdetermined by all the genes. Here the role of coding region (CR) and non-coding region(NCR) of polymerase gene complex is critically reviewed. The coding region of thepolymerase genes can be explained as follows. The amino acid position 627 PB2 gene isa factor adaptation of AI viruses in mammals. Polymerase basic-1 (PB1) protein acts as acentral activity of the enzyme catalyzing the viral polymerase. Polymerase Basic-1 bindsto the terminal end of the vRNA and the cRNA and shows endo-nuclease activity. Theactivity has been identified on the E508, E519, and D522. Polymerase Acid (PA) proteinsplay a role in supporting the biological activity of the polymerase gene complex, but itsmechanism of action in transcription, replication has not been disclosed as clear. Anotherrole of the PA protein is forming a complex of RNA polymerase and express anproteolytic role of cell proteins that suppress cell division. Polymerase acid gene has alsoserine protease activity has been identified at position S624. The non-coding regionmight also play role in the pathogenecity of influenza virus. The results of sequenceanalysis of non-coding region (NCR) at 5?-end of polymerase gene complex of AI virussubtype H5N1 from poultry and pigs in Indonesia showed that the NCR genes PB2, PB1and PA are homogeneous, whereas there are variants of the PB1 gene isolates from ducksA / Duck / Badung, 2006. Occurrence of deletions, insertions, and mutations in the NCRand CR polymerase complex genes may likely lead to genetic changes in viruses whichpotentially also change the nature of biology of AI virus. The study on the 3?-end of thegenes needs to be carried out.