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KAJIAN HASIL PENILAIAN ORGANOLEPTIK PRODUK TAHU YANG DIBUAT DENGAN MENGGUNAKAN KOAGULAN BAKTERI ASAM LAKTAT (BAL) Wati, Dina; Wahyuni, Sri; Khaeruni, Andi
Jurnal Sains dan Teknologi Pangan Vol 2, No 6 (2017): JURNAL SAINS DAN TEKNOLOGI PANGAN
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

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Abstract

ABSTRACT  The purpose of this study was to analyze the effect of the addition of lactic acid bacteria isolates (UM 1.4 A)originating from wikau maombo product on the organoleptik assessment (color, aroma, taste, and texture) of atofu. This study used a completely randomized design (CRD) of one factor with three treatments, ie. 2% lactic acidbacteria, 3% lactic acid bacteria, and 4% lactic acid bacteria. The best result for organoleptic assessment wasobtained by the sample with 4% lactic acid bacteria with with favorite rating scores of color, aroma, taste, andtexture reached 2.813 (slightly like), 3.080 (like), 4.387 (like), and 3.507 (like), respectively.   Keywords: Soy, tofu, Lactic Acid Bacteria, wikau maombo.  ABSTRAK Tujuan dari penelitian ini untuk mengetahui pengaruh penambahan isolat BAL (UM1.4A) asal produk wikaumaombo terhadap karakteristik penilaian organoleptik yang meliputi warna, aroma, rasa dan tekstur pada produktahu. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan penambahan starter BALyaitu penambahan BAL 2%, penambahan BAL 3% dan penambahan BAL 4%. Perlakuan penambahan BAL 4%merupakan perlakuan yang paling disukai panelis dengan skor penilaian kesukaan terhadap warna sebesar2,813 (agak suka), aroma sebesar 3,080 (suka), rasa sebesar 4,387 (suka) dan tekstur sebesar 3,507 (suka).Sehingga dapat disimpulkan bahwa produk tahu dengan perlakuan penambahan BAL 4% dapat diterima dandisukai oleh panelis. Kata kunci: Kedelai, tahu, Bakteri Asam Laktat, wikau maombo.
DETEKSI POTYVIRUS PADA NILAM (Pogostemon Cablin (BLANCO) BENTH) DENGAN TEKNIK ELISA DI SULAWESI TENGGARA TAUFIK, MUHAMMAD; HASAN, ASMAR; KHAERUNI, ANDI; -, GUSNAWATY HS -; MAMMA, SARAWA
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Mosaic symptoms were observed on Patchouli (Pogostemon cablin) around North Kolaka and Kolaka, Southeast Sulawesi during surveys in early 2012. Indirect-ELISA based detection was conducted using symptomatic leaf samples. The objective of the research was to determine disease incidence of Potyvirus in several farms of Patchouli plant in Southeast Sulawesi. The results showed that Patchouli plant (Pogostemon cablin) was found to be infected with Potyvirus disease showing mosaic symptoms and malformation on the leaf samples i.e. in Amotowo and Boro-Boro Villages of subdistrict Boro-Boro, and Landabaro Village, Mowila subdistrict of South Konawe regency; Asinua Village of subdistrict Unaaha, Lambuya district of subdistrict Lambuya, and Bungguosu district, Konawe subdistrict of Konawe regency; and Anduonohu district, Poasia subdistrict of Kendari regency. This is the first report on Potyvirus infection on patchouli in Southeast Sulawesi. Keywords: Inderect-ELISA, mosaic, Potyvirus, Pogostemon cablin
EKSPLORASI DAN KARAKTERISASI AZOTOBACTER INDIGENOUS UNTUK PENGEMBANGAN PUPUK HAYATI TANAMAN PADI GOGO LOKAL DI LAHAN MARJINAL NURMAS, ANDI; -, NOFIANTI; RAHMAN, ABDUL; KHAERUNI, ANDI
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Azotobacter is bacterium that has the ability to fix nitrogen and produce hormone IAA, thus potentially acts as biofertilizer agent. There are differences in chemical, biological and other characters of Azotobacter strains. Some strains have higher ability to fix nitrogen than the others. Exploration and characterization are important to be done because the bacteria that provide nutrients and live free rhizosphere regions, possible have different abilities.  In addition to N2 fixing, growth hormone production, phosphate dissolving, they are also tolerant to a certain temperature and pH. The purpose of the research was to obtain information and the latest data on indigenous Azotobacter that potential as a source of biofertilizer, and triggering factor for upland rice growth on marginal lands in Southeast Sulawesi.  Exploration from several locations has selected 21 Azotobacter isolates. The test results indicated that the 21 Azotobacter isolates have the ability to produce IAA, dissolve phosphate and stable at pH 5.0-7.0. All 21 isolates tested had the ability to survive at 40oC, eight isolates i.e. LT2D1, LT2d2, LU2c, RG4c, MP1f, LT2d3, ML1j, and RR8awere able to survive at a temperature of 45o C, and LT2d1 isolate survived at temperatures 50oC. The results of the evaluation of the wet weight of upland rice seedlings selected 10 isolates that were: KU6e, MS3e, RG4c, RR8b1, LU2c, RB4b, MS3f, LU2c1, RJ5e, RR8b2 and evaluation of seedling dry weight selected 5 isolates that were: RB4b, LU2c, RJ5e, RR8b2, LT2d1. Keywords:      exploration, characterization, indigenous Azotobacter, local upland rice
PENGGUNAAN BAKTERI KITINOLITIK SEBAGAI AGENS BIOKONTROL PENYAKIT BUSUK BATANG OLEH RHIZOCTONIA SOLANI PADA TANAMAN KEDELAI (UTILIZATION OF CHITINOLITIC BACTERIA AS BIOCONTROL AGENT OF STEM ROT DISEASE BY RHIZOCTONIA SOLANI ON SOYBEAN) Khaeruni, Andi; Rahman, Abdul
Jurnal Fitopatologi Indonesia Vol 8 No 2 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.706 KB) | DOI: 10.14692/jfi.8.2.37

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Biological control of stem rot disease caused by Rhizoctonia solani on soybean using chitinolitic bacteria is one of the environmentally friendly control methods of pathogen. The study was conducted to select chitinolitic bacteria and to test their ability to suppress stem rot disease and promote plant growth of soybean. A total of ten chitinolitic bacteria were tested in vitro, and five of isolates i.e: ST21e, SS12b, ST17c, ST27d, and ST26c found to have more than 30% suppression toward R. solani. In planta test under green house condition at six weeks after planting showed that soybean plants inoculated with ST27d and ST17c isolates had significantly lower disease incidence and had increased plant height and leaf number. Both of chitinolitic bacteria isolates are promising as biocontrol agents of stem rot disease caused by R. solani of soybean.Key words: biological control, chitinolitic bacteria, Rhizoctonia solani
Keragaman Genetika Xanthomonas axonopodis pv. glycines Asal Kedelai Varietas Edamame di Indonesia Khaeruni, Andi; Suwanto, Antonius; Tjahjono, Budi; Sinaga, Meity S.
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11814

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Xanthomonas axonopodis pv. glycines cause bacterial pustule disease caused a serious disease in Edamame cultivation in Indonesia. We collected a total of 29 X. axonopodis pv. glycines isolates from Edamame fields at Jember, Ciawi, Cipanas and Bogor. The genetic diversity analysis of all isolates employing ARDRA and ISR technique showed six and seven different DNA profile, respectively. Therefore there are at least seven strains of X. axonopodis pv. glycines infected Edamame in Indonesia. Both CPI from Cipanas and JA4 from Sukorejo Jember isolates possess unique DNA profle and genetically are not closely related to other isolates.
KETAHANAN BERBAGAI KULTIVAR PADI LOKAL TERHADAP PENYAKIT HAWAR DAUN BAKTERI Khaeruni, Andi; Najamuddin, Erwin; Wijayanto, Teguh; Syair, Syair
Jurnal Fitopatologi Indonesia Vol 12 No 3 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.799 KB) | DOI: 10.14692/jfi.12.3.89

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Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae is an important rice disease in Indonesia, including in South East Sulawesi. The use of resistant varieties is one of the effective and environmentally friendly control strategies to suppress the disease. This study aimed to determine the level of resistance of some local rice varieties of South and Southeast Sulawesi against Xanthomonas oryzae pv. oryzae pathotypes IV, VIII and X. The study was conducted in a screen house involving 3 bacterial pathotypes and 11 local rice cultivars. Shearing method was used for inoculation of bacteria to leaf on vegetative and generative phases. Disease incidences were measured 3 weeks after inoculation, and the data was further used to determine the level of resistance of the tested rice cultivars. The results showed that incubation period of the disease was longer on Kelaca cultivar compared to other cultivars. On vegetative phase, this cultivar showed moderate resistant to pathotypes IV and X, and highly resistant to patotype VIII, whereas on the generative phase it showed moderate resistant to pathotypes IV and VII, and highly resistant to pathotype X. Therefore, Kelaca cultivar can be recommended for endemic areas of leaf blight in South and Southeast Sulawesi
Keefektifan Waktu Aplikasi Formulasi Rizobakteri Indigenus untuk Mengendalikan Layu Fusarium dan Meningkatkan Hasil Tanaman Tomat di Tanah Ultisol Khaeruni, Andi; Wahab, Abdul; Taufik, Muhammad; Sutariati, Gusti Ayu Kade
Jurnal Hortikultura Vol 23, No 4 (2013): Desember 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v23n4.2013.p365-371

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Layu fusarium merupakan salah satu penyakit penting di pertanaman tomat, termasuk di lahan Ultisol di Sulawesi Tenggara. Rizobakteri pemacu pertumbuhan tanaman merupakan salah satu alternatif pengendalian penyakit yang menjanjikan untuk mengatasi masalah layu fusarium. Penelitian bertujuan mengetahui keefektifan formulasi rizobakteri indigenus untuk mengendalikan layu fusarium dan meningkatkan hasil panen tanaman tomat di tanah Ultisol. Penelitian dilaksanakan di Laboratorium Agroteknologi dan Rumah Kasa, Fakultas Pertanian, Universitas Halu Oleo dari Bulan Oktober 2010 sampai Januari 2011. Penelitian disusun berdasarkan rancangan acak lengkap dengan tujuh perlakuan, yaitu (RB) aplikasi rizobakteri pada benih, (RB.2) aplikasi rizobakteri pada benih dan pada tanaman 2 minggu setelah tanam, (RB.2.4) aplikasi rizobakteri pada benih dan pada tanaman 2 dan 4 minggu setelah tanam, (RBF.2) aplikasi rizobakteri pada benih dan aplikasi fungisida sintetik pada tanaman 2 minggu setelah tanam, (FB.4) aplikasi fungisida sintetik pada benih dan pada tanaman 2 minggu setelah tanam, (FB.2.4) aplikasi fungisida sintetik pada benih dan pada tanaman 2 dan 4 minggu setelah tanam, serta kontrol. Semua perlakuan diinokulasi dengan Fusarium oxysporum f. sp. lycopersici dan diulang tiga kali. Hasil penelitian menunjukkan bahwa perlakuan rizobakteri pada benih yang disusul pada saat 2 dan 4 minggu setelah tanam paling efektif mengendalikan layu fusarium dengan penekanan sebesar 61,14%, serta mampu meningkatkan hasil tanaman dengan jumlah buah 10,88 dan bobot buah 375,31 g per tanaman, sedangkan tanaman tanpa perlakuan tidak berproduksi. Hasil ini mengindikasikan bahwa formulasi rizobakteri indigenus mampu mengendalikan layu fusarium dan meningkatkan hasil panen tanaman tomat di tanah Ultisol.
Agensia hayati dan Arachis pintoi memacu pertumbuhan tanaman lada (Piper nigrum) dan mengurangi kejadian penyakit kuning Biocontrol agents and Arachis pintoi promote the growth of black pepper (Piper nigrum) and reduce the incidence of yellow disease TAUFIK, Muhammad; KHAERUNI, Andi; WAHAB, Abdul; AMIRUDDIN, .
E-Journal Menara Perkebunan Vol 79, No 2: Desember 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v79i2.58

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AbstractYellow disease is a complex disease caused byFusarium sp., Phytophthora sp., and nematodes. Infectedplants were quickly killed and were difficult for replanting,causing significant losses for the growers. Various controlmethods were examined including the use of bioconrolagents and cover crop Arachis pintoi. The researchobjective was to determine the ability of biocontrol agentsand A. pintoi to improve pepper growth and reduce yellowdisease incidence on pepper plants in the field. Researchresults showed that the treatment of biocontrol andA. pintoi promoted vegetative growth of pepper plants, andincreased pepper height for up to more five times, andreduced yellow disease incidence to 30%AbstrakPenyakit kuning merupakan penyakit kompleks yangdisebabkan oleh Fusarium sp., Phytophthora sp. dannematoda parasit. Tanaman sakit mengalami kematianyang cepat dan kebun yang telah terinfeksi sulit untukditanami kembali, sehingga mengakibatkan kerugian yangnyata terhadap petani. Berbagai cara pengendalian telahdiuji termasuk penggunaan agens hayati Plant GrowthPromoting Rhizobacteria (PGPR), Trichoderma sp. dantanaman Arachis pintoi. Tujuan penelitian adalahmengetahui kemampuan agensia hayati dan Arachis pintoidalam meningkatkan pertumbuhan dan mengurangikejadian penyakit kuning pada tanaman lada di lapang.Hasil penelitian menunjukkan bahwa perlakuan agenshayati dan A. pintoi meningkatkan tinggi dan jumlah dauntanaman lada lebih dari lima kali serta mempercepatmunculnya sulur dibandingkan dengan kontrol danfungisida. Aplikasi Trichoderma sp. yang dikombinasidengan A. pintoi menekan kejadian penyakit kuning hampir30%.
INTEGRASI TEKNIK INVIGORASI BENIH DENGAN RIZOBAKTERI UNTUK PENGENDALIAN PENYAKIT DAN PENINGKATAN HASIL TOMAT Sutariati, Gusti Ayu Kade; Madiki, Abdul; Khaeruni, Andi
Jurnal Fitopatologi Indonesia Vol 10 No 6 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.714 KB) | DOI: 10.14692/jfi.10.6.188

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The effects of pre-planted seed invigoration treatment integrated with rhizobacteria were studied in an attempt to control fusarium wilt disease and to improve yield of tomato in the field. Seed treatments consisted of seed invigoration with single or mixture rhizobacteria, burned rice husk or sawdust seed matriconditioning, integrated seed matriconditioning with single or mixture rhizobacteria, seed hydration, and fungicide seed treatment. The result showed that the most effective treatment was the integration of rice hull charcoal seed matriconditioning with Bacillus polymixa BG25. The seed treatment increased total fruit and fruit weight by 40% and 166% , respectively. The treatment was also effective in suppressing disease incidence by 70%. Based on this experiment, rhizobacteria B. polymixa BG25 integrated with rice hull charcoal seed matriconditioning can be recommended as biocontrol agents in improving yield and controlling fusarium wilt disease of tomato. 
Induksi Ketahanan terhadap Penyakit Hawar Daun Bakteri pada Tanaman Padi di Lapangan Menggunakan Rizobakteri Indigenos Khaeruni, Andi; Rahim, Abdul; ., Syair; ., Adriani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 14, No 1 (2014): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.781 KB) | DOI: 10.23960/j.hptt.11457-63

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ABSTRACTInduced resistance to bacterial leaf blight disease in rice field by indigenous rhizobacteria.  Bacterial leaf blight is the most important disease on paddy at Southeast Sulawesi. Utilization of biological agents that induce plant resistance is an alternative tool to control bacterial leaf blight disease on paddy. The aim of  the experiment was obtain rhizobacteria that were able to stimulate the growth of paddy plants as well induce plant resistance towards bacterial leaf blight in the field. All experiment units were arranged with a factorial design in a randomized complete block design. The first factor was the rhizobacteria isolates, consisting 4 treatments, i.e: without rhizobacteria (R0), isolate P11a (R1), isolate PKLK5 (IR2), and  mixture P11a and PKLK5 (R3), the second factor is paddy varieties, (V1): IR64 variety, (V2): Cisantana variety. The pathogen was inoculated on leaf when 45 day after crop.  Weekly observation of disease severity, vegetative plant growth (leaf and stem numbers), and yield were conducted. The results showed that the 10 isolates of rhizobacteria tested were able to induce plant resistance toward bacterial leaf blight, stimulated vegetative growth as well as increased yield of paddy plant. Rhizobacteria application could increase the resistance of paddy toward Xanthomonas oryzae pv. oryzae. The application rhizobacteria could increase the vegetative plant growth, application  mixture P11a and PKLK5 isolates showed higher resistance than single application in terms of plant growth and yield, both IR64 and Cisantana varietes