Yuktiana Kharisma
Fakultas Kedokteran Universitas Islam Bandung

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Labu Kuning (Cucurbita moschata Durch.) untuk Penurunan Kadar Glukosa Darah Puasa pada Tikus Model Diabetik Fathonah, Rahmi; Indriyanti, Anita; Kharisma, Yuktiana
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak   Diabetes melitus didefinisikan sebagai penyakit metabolik yang ditandai dengan hiperglikemia akibat defisiensi insulin atau penurunan efektivitas insulin dan dapat menimbulkan berbagai komplikasi akut maupun kronik. Salah satu obat tradisional yang mempunyai efek anti diabetik adalah labu kuning (Cucurbita moschata Durch.) yang mengandung flavonoid, beta-karoten, vitamin C, dan vitamin E. Tujuan penelitian ini adalah untuk mengetahui efek dan rentang dosis efektif ekstrak air labu kuning terhadap penurunan kadar glukosa darah puasa pada tikus model diabetik. Penelitian ini bersifat eksperimental laboratorik dengan menggunakan disain rancangan acak lengkap terhadap 28 ekor tikus putih jantan galur Wistar yang terbagi dalam empat kelompok yaitu kelompok I (diet biasa, induksi aloksan), kelompok II (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 56 mg/200grBB/hari per oral), kelompok III (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 112 mg/200grBB/hari per oral), dan kelompok IV (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 224 mg/200grBB/hari per oral). Pengukuran kadar glukosa darah puasa dilakukan setelah masa adaptasi, setelah diinduksi aloksan, hari ke-7 dan hari ke-14 perlakuan. Data dianalisis dengan menggunakan uji repeated analysis of varians (ANOVA) lalu dilanjutkan dengan uji post-hoc Tamhane’s T2. Hasil penelitian menunjukan bahwa pemberian ekstrak air labu kuning dengan rentang dosis 56 mg/200grBB/ hari per oral sampai 112 mg/200grBB/hari per oral selama 14 hari dapat menurunkan kadar glukosa darah puasa dengan bermakna. Penurunan kadar glukosa darah puasa diduga karena labu kuning mengandung flavonoid, beta-karoten, vitamin C dan vitamin E.  Kata kunci : Diabetes melitus,ekstrak air labu kuning, glukosa darah puasa Pumpkin (Curcubita moschata Durch) to Decline of Blood Glucose Fasting Levels in Diabetic Mice   Abstract   Diabetes mellitus defined as syndrome of metabolic diseases characterized by hyperglycemia due to insulin deficiency or decreased effectiveness of insulin that cause various acute and chronic complications. One of the traditional medicines which have anti-diabetic effect is pumpkin (Cucurbita moschata Durch) which contains flavonoids, beta-carotene, vitamin C, and vitamin E. The purpose of this study is to determine the effects and the effective dose range of pumpkin water extracts to the decline of blood glucose fasting levels in diabetic mice. This study was an  experimental research with complete randomized design using 28 white male wistar mice divided into four groups i.e. group I (normal diet, alloxan induce), group II (normal diet, alloxan induce, water extract of pumpkin at the dose 56 mg/200grBW/day orally), group III (normal diet, alloxan induce,  pumpkin water extract with the dose of 112 mg/200grBW/day orally), and group IV (normal diet, alloxan induce, pumpkin water extract orally with the dose of 224 mg/200grBW/day). Measurement of blood glucose fasting levels done after the adaptation period, after alloxan induced, on day 7th and day 14th of treatment. Data was analyzed using repeated ANOVA test followed by post-hoc test. The results showed that administration of pumpkin water extract with dose ranges of 56 mg/200grBB/day orally to 112 mg/200grBB/day orally for 14 days can lower blood glucose fasting levels. The decrease in blood glucose fasting levels presumably was because pumpkin contains flavonoid, beta-carotene, vitamin C and vitamin E which known to have those effects.   Key words : Blood glucose fasting level, diabetes mellitus,pumpkin water extract 
Bekatul (Oryza sativa L) untuk Menghambat Peningkatan Kadar Kolesterol Darah Kania, Astri; Kharisma, Yuktiana; Dewi, Miranti Kania
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Islam Bandung

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Abstract

Abstrak Penyakit kardiovaskuler merupakan penyebab utama kematian dan kecacatan di seluruh dunia, termasuk Indonesia. Penyakit kardiovaskuler disebabkan oleh pembentukan aterosklerosis pada pembuluh darah. Konsumsi larutan tepung bekatul (Oryza sativa L) dapat digunakan sebagai salah satu upaya untuk mencegah terjadinya aterosklerosis dengan cara menghambat peningkatan kadar kolesterol darah. Penelitian ini untuk menilai efek larutan tepung bejatul terhadap penghambatan peningkatan kadar kolesterol darah. Penelitian eksperimental laboratorik dengan rancangan acak lengkap telah dilakukan pada 20 ekor tikus putih jantan galur Wistar yang dibagi menjadi lima kelompok. Kelompok I adalah kontrol negatif yang diberikan diet tinggi lemak dan propiltiurasil (PTU) 0,01%, kelompok II merupakan kontrol positif yang hanya diberi pelet. Kelompok III, IV, dan V adalah kelompok perlakuan yang diberikan sediaan uji 0,27 gram/200 gram BB, 0,54 gram/200 gram BB, dan 1,08 gram/200 gram BB, DTL dan PTU 0,01% secara bersamaan. Pengukuran kadar kolesterol darah dilakukan sebelum diberikan perlakuan (hari ke-7) dan setelah diberikan perlakuan (hari ke-21) dengan menggunakan metode kolorimetrik enzimatik. Hasil penelitian ini menunjukkan bahwa larutan tepung bekatul memiliki efek penghambatan terhadap peningkatan kadar kolesterol darah dengan dosis 0,54 gram/200 gram BB. Efek penghambatan tersebut diakibatkan oleh mekanisme interaksi  dari orizanol, fitosterol, dan omega-3 yang terdapat dalam larutan tepung bekatul.  Kata kunci : Bekatul, efek penghambatan, kolesterol darah Rice Bran (Oryza sativa L.) Inhibit the Increase of Blood Cholesterol Level Abstract Cardiovascular disease is the major cause of mortality and morbidity in the world, including Indonesia. This disease caused by atherosclerosis formation in blood vessel. Consumption of rice bran is one alternative to prevent atherosclerosis by inhibiting the increase of blood cholesterol level. The Aim of study was to analyze rice bran in inhibiting the increase of blood choelsterol level. The laboratory experimental study with complete randomized design has been conducted to 20 male mice Wistar strain divided into five groups. Group I is the negative control group with high-fat diet and 0,01 % propylthiouracil (PTU), group II is the positive control group with standard diet, and group III, IV and V are the treatment group which were given 0,27 gram/200 gram of BW, 0,54 gram/200 gram of BW, and 1,08 gram/200 gram of BW, DTL, and  0,01 % PTU.  The measurement of the blood cholesterol rate conducted before treatment (7th days) and after treatment (21st days)  using enzymatic colorimetric method. The result showed that the dose of rice bran solution inhibit the increased of blood cholesterol level at 0,54 gram/200 gram of BW. Inhibitory effect was caused by the interaction mechanism betweem oryzanol, phytosterol, and omega-3 that are in the rice bran solution. Key words : Blood cholesterol, cardiovascular, inhibition effect,  rice bran 
Penentuan Derajat Toksisitas Akut Ekstrak Air Buah Pepaya (Carica papaya L.) Muda Pada Mencit Menggunakan Purposed New Recommended Method Nadiyah, Laila Dinda; Kharisma, Yuktiana; Yuniarti, Yuniarti
Jurnal Jamu Indonesia Vol 1 No 2 (2016): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (816.205 KB) | DOI: 10.29244/jji.v1i2.14

Abstract

Papaya (Carica papaya L.) fruit was proven scientifically in having medicinal effects: such as gastroprotective, laxative, galactagogues, and antibacterial effect. Unripe papaya fruit contains saponin, alkaloid, tannin, flavonoid, triterpenoid, and quinone which may reveal toxicities as adverse effect when it consumed in high dose. The aim of this study is to determine the acute toxicity degree of unripe Carica papaya L. fruit aqueous extract in mice using proposed new recommended method. The acute toxicity degree conducted by determination of lethal dose50 (LD50) using new recommended  method to 12 female mice. The subjects were divided into three stages and each mouse was given different dose of the extract. LD50 calculated by mean of mortality lowest dose and non mortality highest dose then the result classified into the degree of acute toxicity. The study showed there was no mortality in all groups of the experimental subject. LD50 of unripe Carica papaya L. aqueous extract was found >5000 mg/kg BW and classified as Practically Non Toxic (PNT) substance.
Community Knowledge and Behavior in the Utilization of Medicinal Plants in Cikoneng Village Bandung District Rahimah, Santun Bhekti; Kharisma, Yuktiana; Nurhayati, Eka; Yuniarti, Yuniarti; Santoso, Shenny Dianathasari; Faridza, Muhammad
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v7i1.3214

Abstract

The industry of the traditional medicinal made from medicinal plants was currently growing. Effortless cultivation and utilization of medicinal plants were an important step to preserve the traditional medicine of Indonesia. Cikoneng village had abundant natural potential and is an assisted village of the researchers' institution located at the foot of Manglayang mountain Bandung district. Therefore, the researchers implemented the intervention program to educate and socialize the use of medicinal plants to the community of Cikoneng village. After the intervention program, the assessment of the level of knowledge and perceptions of people in the behavior of treatment by medicinal plants utilized was carried out. This study aims to assess the increase in knowledge and perceptions of people in the behavior of cultivation and treatment by using medicinal plants in Cikoneng village. The study used an intervention program and questionnaire with 35 respondents conducted on 22 August–23 September 2016. The results showed that after the intervention program, the level of knowledge of the Cikoneng village community regarding medicinal plants was right. The entire people of Cikoneng village is willing to take advantage of medicinal plants in maintaining family health and will begin to cultivate them in the smallest scope (family). In conclusion, there is an increase in people's knowledge and perception of the behavior of cultivation and treatment by utilizing medicinal plants in Cikoneng village. PENGETAHUAN DAN PERILAKU MASYARAKAT DALAM PEMANFAATAN TANAMAN OBAT DI KAMPUNG CIKONENG KABUPATEN BANDUNGIndustri obat tradisional berbahan baku tanaman obat saat ini semakin berkembang. Upaya budidaya dan pemanfaatan tanaman obat yang optimal merupakan langkah penting untuk menjaga kelestarian obat tradisional Indonesia. Kampung Cikoneng mempunyai potensi alam yang melimpah dan merupakan desa binaan institusi peneliti yang terletak di kaki Gunung Manglayang Kabupaten Bandung. Oleh karena itu, peneliti melaksanakan program intervensi untuk mengedukasi dan menyosialisasikan pemanfaatan tanaman obat kepada masyarakat Kampung Cikoneng. Pada akhir program intervensi, dilakukan penilaian tingkat pengetahuan dan persepsi masyarakat tentang perilaku pengobatan dengan tanaman obat. Penelitian ini bertujuan menilai peningkatan pengetahuan dan persepsi masyarakat tentang perilaku budidaya dan pengobatan dengan memanfaatkan tanaman obat di Kampung Cikoneng. Penelitian menggunakan program intervensi dan kuesioner dengan jumlah responden 35 orang yang dilaksanakan pada 22 Agustus–23 September 2016. Hasil memperlihatkan bahwa setelah program intervensi, tingkat pengetahuan masyarakat Kampung Cikoneng mengenai tanaman obat adalah baik. Seluruh masyarakat Kampung Cikoneng bersedia memanfaatkan tanaman obat dalam menjaga kesehatan keluarga dan akan mulai membudidayakannya dalam lingkup yang paling kecil (keluarga). Simpulan, terdapat peningkatan pengetahuan dan persepsi masyarakat tentang perilaku pengobatan dengan memanfaatkan tanaman obat di Kampung Cikoneng.
Labu Kuning (Cucurbita moschata Durch.) untuk Penurunan Kadar Glukosa Darah Puasa pada Tikus Model Diabetik Fathonah, Rahmi; Indriyanti, Anita; Kharisma, Yuktiana
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v2i1.1527

Abstract

Diabetes melitus didefinisikan sebagai penyakit metabolik yang ditandai dengan hiperglikemia akibat defisiensi insulin atau penurunan efektivitas insulin dan dapat menimbulkan berbagai komplikasi akut maupun kronik. Salah satu obat tradisional yang mempunyai efek anti diabetik adalah labu kuning (Cucurbita moschata Durch.) yang mengandung flavonoid, beta-karoten, vitamin C, dan vitamin E. Tujuan penelitian ini adalah untuk mengetahui efek dan rentang dosis efektif ekstrak air labu kuning terhadap penurunan kadar glukosa darah puasa pada tikus model diabetik. Penelitian ini bersifat eksperimental laboratorik dengan menggunakan disain rancangan acak lengkap terhadap 28 ekor tikus putih jantan galur Wistar yang terbagi dalam empat kelompok yaitu kelompok I (diet biasa, induksi aloksan), kelompok II (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 56 mg/200 gBB/hari per oral), kelompok III (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 112 mg/200 gBB/hari per oral), dan kelompok IV (diet biasa, induksi aloksan, ekstrak air labu kuning dosis 224 mg/200 gBB/hari per oral). Pengukuran kadar glukosa darah puasa dilakukan setelah masa adaptasi, setelah diinduksi aloksan, hari ke-7, dan hari ke-14 perlakuan. Data dianalisis dengan menggunakan uji repeated analysis of varians (ANOVA) lalu dilanjutkan dengan uji post-hoc Tamhane’s T2. Hasil penelitian menunjukan bahwa pemberian ekstrak air labu kuning dengan rentang dosis 56 mg/200 gBB/ hari per oral sampai 112 mg/200 gBB/hari per oral selama 14 hari dapat menurunkan kadar glukosa darah puasa dengan bermakna. Penurunan kadar glukosa darah puasa diduga karena labu kuning mengandung flavonoid, beta-karoten, vitamin C dan vitamin E. PUMPKIN (CURCUBITA MOSCHATA DURCH.) TO DECLINE OF BLOOD GLUCOSE FASTING LEVELS IN DIABETIC MICEDiabetes mellitus defined as syndrome of metabolic diseases characterized by hyperglycemia due to insulin deficiency or decreased effectiveness of insulin that cause various acute and chronic complications. One of the traditional medicines which have anti-diabetic effect is pumpkin (Cucurbita moschata Durch.) which contains flavonoids, beta-carotene, vitamin C, and vitamin E. The purpose of this study is to determine the effects and the effective dose range of pumpkin water extracts to the decline of blood glucose fasting levels in diabetic mice. This study was an experimental research with complete randomized design using 28 white male wistar mice divided into four groups i.e. group I (normal diet, alloxan induce), group II (normal diet, alloxan induce, water extract of pumpkin at the dose 56 mg/200 gBW/day orally), group III (normal diet, alloxan induce,  pumpkin water extract with the dose of 112 mg/200 gBW/day orally), and group IV (normal diet, alloxan induce, pumpkin water extract orally with the dose of 224 mg/200 gBW/day). Measurement of blood glucose fasting levels done after the adaptation period, after alloxan induced, on day 7th and day 14th of treatment. Data was analyzed using repeated ANOVA test followed by post-hoc test. The results showed that administration of pumpkin water extract with dose ranges of 56 mg/200 gBB/day orally to 112 mg/200 gBB/day orally for 14 days can lower blood glucose fasting levels. The decrease in blood glucose fasting levels presumably was because pumpkin contains flavonoid, beta-carotene, vitamin C and vitamin E which known to have those effects.
Bekatul (Oryza sativa L.) Menghambat Peningkatan Kadar Kolesterol Darah Kania, Astri; Kharisma, Yuktiana; Dewi, Miranti Kania
Global Medical & Health Communication (GMHC) Vol 2, No 1 (2014)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v2i1.1528

Abstract

Penyakit kardiovaskuler merupakan penyebab utama kematian dan kecacatan di seluruh dunia, termasuk Indonesia. Penyakit kardiovaskuler disebabkan oleh pembentukan aterosklerosis pada pembuluh darah. Konsumsi larutan tepung bekatul (Oryza sativa L.) dapat digunakan sebagai salah satu upaya untuk mencegah terjadinya aterosklerosis dengan cara menghambat peningkatan kadar kolesterol darah. Penelitian ini untuk menilai efek larutan tepung bejatul terhadap penghambatan peningkatan kadar kolesterol darah. Penelitian eksperimental laboratorik dengan rancangan acak lengkap telah dilakukan pada 20 ekor tikus putih jantan galur Wistar yang dibagi menjadi lima kelompok. Kelompok I adalah kontrol negatif yang diberikan diet tinggi lemak dan propiltiurasil (PTU) 0,01%, kelompok II merupakan kontrol positif yang hanya diberi pelet. Kelompok III, IV, dan V adalah kelompok perlakuan yang diberikan sediaan uji 0,27 gram/200 gram BB, 0,54 gram/200 gram BB, dan 1,08 gram/200 gram BB, DTL dan PTU 0,01% secara bersamaan. Pengukuran kadar kolesterol darah dilakukan sebelum diberikan perlakuan (hari ke-7) dan setelah diberikan perlakuan (hari ke-21) dengan menggunakan metode kolorimetrik enzimatik. Hasil penelitian ini menunjukkan bahwa larutan tepung bekatul memiliki efek penghambatan terhadap peningkatan kadar kolesterol darah dengan dosis 0,54 gram/200 gram BB. Efek penghambatan tersebut diakibatkan oleh mekanisme interaksi dari orizanol, fitosterol, dan omega-3 yang terdapat dalam larutan tepung bekatul. RICE BRAN (ORYZA SATIVA L.) INHIBIT THE INCREASE OF BLOOD CHOLESTEROL LEVELCardiovascular disease is the major cause of mortality and morbidity in the world, including Indonesia. This disease caused by atherosclerosis formation in blood vessel. Consumption of rice bran is one alternative to prevent atherosclerosis by inhibiting the increase of blood cholesterol level. The aim of study was to analyze rice bran in inhibiting the increase of blood choelsterol level. The laboratory experimental study with complete randomized design has been conducted to 20 male mice Wistar strain divided into five groups. Group I is the negative control group with high-fat diet and 0,01 % propylthiouracil (PTU), group II is the positive control group with standard diet, and group III, IV and V are the treatment group which were given 0,27 grams/200 grams of BW, 0,54 grams/200 grams of BW, and 1,08 grams/200 grams of BW, DTL, and 0,01 % PTU. The measurement of the blood cholesterol rate conducted before treatment (7th days) and after treatment (21st days) using enzymatic colorimetric method. The result showed that the dose of rice bran solution inhibit the increased of blood cholesterol level at 0,54 grams/200 grams of BW. Inhibitory effect was caused by the interaction mechanism betweem oryzanol, phytosterol, and omega-3 that are in the rice bran solution.
Acute Toxicity Test of Unripe Papaya (Carica papaya L.) Aqueous Extract (UPAE) on The Blood Urea and Creatinine Concentration Kharisma, Yuktiana; Andriane, Yuke; Respati, Titik
Global Medical & Health Communication (GMHC) Vol 6, No 2 (2018)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v6i2.3794

Abstract

Unripe papaya aqueous extract (UPAE) widely used as lactation stimulator, antidiabetes, antibacterial, and anti-inflammatory. The utilization of papaya is not known for its safety yet, so it is necessary to research its toxicity. The purpose of this study was to investigate the acute toxicity of UPAE on renal function through measurement of blood urea and creatinine levels. This study was conducted in July 2017 in Laboratory of Medical Biology, Faculty of Medicine, Universitas Islam Bandung. This study used pure in vivo experimental design on 11 Swiss Webster mice using the dose of acute toxicity determination based on new recommended methods of 0; 50; 200; 400; 800; 1,000; 1,500; 2,000; 3,000; 4,000; and 5,000 mg/kgBW. After 24 hours, 1 mL blood drawn through the tail examined for blood urea and creatinine levels. The measurement of urea content using kinetic method point and creatinine level using modified Jaffe method. Provision of UPAE at doses of 0, 50, 200, 400, 800, and 1,000 mg/kgBW resulted on blood urea equal to 39, 35, 48, 49, 48, and 32 mg/dL respectively. Blood urea level 23, 22, 28, 34, and 35 mg/dL was obtained at 1,500 UPAE doses; 2,000; 3,000; 4,000; and 5,000 mg/kgBW dosages respectively. After 24 hours of UPAE administration, the creatinine level in various doses using new recommended method of (0–5,000 mg/kgBW) were 0.75, 0.54, 0.53, 0.50, 0.60, 0.54, 0.52, 0.55, 0.42, 0.51, and 0.40 mg/dL. In conclusion, UPAE do not cause acute toxicity on renal function through measurement of blood urea and creatinine levels. TOKSISITAS AKUT EKSTRAK AIR BUAH PEPAYA (CARICA PAPAYA L.) TERHADAP KADAR UREUM DAN KREATININ DARAHEkstrak air buah pepaya muda (EABPM) digunakan secara empiris sebagai laktagogum, antidiabetes, antibakteri, dan antiinflamasi. Tingkat keamanannya belum banyak diketahui sehingga perlu dilakukan penelitian uji toksisitas akut. Tujuan penelitian ini adalah mengetahui toksisitas akut EABPM terhadap fungsi ginjal melalui pengukuran kadar ureum dan kreatinin plasma. Penelitian ini dilaksanakan pada bulan Juli 2018 di Laboratorium Biologi Medis, Fakultas Kedokteran, Universitas Islam Bandung. Penelitian ini menggunakan desain eksperimental murni in vivo terhadap 11 ekor mencit betina galur Swiss Webster dengan penentuan dosis sesuai dengan new recommended method: 0, 50, 200, 400, 800, 1.000, 1.500, 2.000, 3.000, 4.000, dan 5.000 mg/kgBB. Setelah 24 jam, diambil darah melalui ekor mencit sebanyak 1 mL untuk diperiksa kadar ureum dan kreatinin plasma. Pengukuran kadar ureum menggunakan point kinetic method dan kadar kreatinin menggunakan metode Jaffe yang dimodifikasi. Pemberian EABPM pada dosis 0, 50, 200, 400, 800, dan 1.000 mg/kgBB didapatkan kadar ureum plasma 39, 35, 48, 49, 48, dan 32 mg/dL secara berurutan. Kadar ureum plasma 23, 22, 28, 34, dan 35 mg/dL didapatkan pada pemberian dosis EABPM sebanyak 1.500, 2.000, 3.000, 4.000, dan 5.000 mg/kgBB. Kadar kreatinin plasma dalam berbagai dosis (0–5.000 mg/kgBB) adalah 0,75; 0,54; 0,53; 0,50; 0,60; 0,54; 0,52; 0,55; 0,42; 0,51; dan 0,40 mg/dL. Simpulan, EABPM tidak menimbulkan tanda toksisitas akut pada fungsi ginjal melalui pengukuran kadar ureum dan kreatinin plasma.
Toksisitas Akut Ekstrak Air Buah Pepaya (Carica papaya L.) Muda terhadap Morfologi Eritrosit Kharisma, Yuktiana; Hendryanny, Eka; Riani, Astari P.
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v5i2.2280

Abstract

Pepaya (Carica papaya L.) adalah salah satu obat tradisional yang digunakan untuk mengatasi masalah kesehatan. Pepaya mengandung beberapa substansi fitokimia seperti saponin, alkaloid, terpenoid, dan flavonoid. Saponin dan alkaloid diketahui mampu berinteraksi dengan membran eritrosit dan menyebabkan disintegrasi membran sehingga dapat menyebabkan kerusakan pada eritrosit. Penelitian ini bertujuan mengetahui toksisitas akut ekstrak air buah pepaya muda terhadap morfologi eritrosit melalui pengamatan sediaan apus darah tepi. Metode penelitian ini adalah eksperimental laboratorium dilakukan di Laboratorium Biomedis, Fakultas Kedokteran, Universitas Islam Bandung periode Januari–Februari 2016. Penentuan kelompok dosis berdasar atas proposed (new) recommended method menggunakan 11 ekor tikus yang diberi dosis oral ekstrak air buah pepaya muda 50, 200, 400, 800, 1.000, 1.500, 2.000, 3.000, 4.000, 5.000 mg/kgBB masing-masing dan satu tikus hanya diberikan air sebagai kelompok kontrol. Pengamatan dilakukan setelah 24 jam pemberian ekstrak. Hasil pengamatan sediaan apus darah tepi menunjukkan tidak terdapat perubahan morfologi eritrosit baik bentuk, ukuran, dan warnanya. Simpulan, ekstrak air buah pepaya muda tidak memiliki toksisitas akut terhadap morfologi eritrositACUTE TOXICITY OF UNRIPE PAPAYA FRUIT (CARICA PAPAYA L.) WATER EXTRACT TO MORPHOLOGY OF ERYTHROCYTEPapaya (Carica papaya L.) is one of traditional medicines which was used to overcome health things. It is contained of some phytochemicals substance such as saponin, alkaloid, terpenoid, and flavonoid. Both saponin and alkaloid were known having an ability to interract with eryhtrocyte membran and cause membrane disintegrity that can destruct the erythrocyte. This study was aimed to know the acute toxicity of the aqueous extract of unripe papaya fruit to the erythrocyte morphology by observe the peripheral blood smear. The method of this study was conducted experimental laboratory at Loboratory of Biomedical, Faculty of Medicine, Universitas Islam Bandung in January–February 2016. Determination of dose group is based on proposed (new) recommended method with 11 rats were administrated oral dose 50, 200, 400, 800, 1,000, 1,500, 2,000, 3,000, 4,000, 5,000 mg/kgBW of unripe papaya fruit aqueous extract, and one rat was only given water as control group. Observation had been done at 24 hours after extract administrating. It showed that there was not any abnormal morphology, size, and chromatic changes of erythrocyte in blood smear observations. In conclusion, the aqueous extract of unripe papaya fruit do not have the acute toxicity to erythrocyte morphology.
Folic Acid Usual Doses Decrease the Buccal Micronucleus Frequency on Smokers Kharisma, Yuktiana; Damayanti, Meta Maulida; Yulianto, Fajar Awaliya; Rahimah, Santun Bhekti; Maharani, Winni; Rachmawati, Meike; Sastramihardja, Herri S.; Abdul ?Aziiz, Muhammad Alief; Halim, Muhammad Ilham
Global Medical & Health Communication (GMHC) Vol 7, No 2 (2019)
Publisher : Global Medical & Health Communication (GMHC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v7i2.4414

Abstract

Cigarette contains toxic chemical compounds that trigger DNA instability. Initial genotoxic oral cavity characterized by the appearance of micronucleus (MN) in the buccal mucosa. Folate is needed in maintaining DNA stability. This study aimed to compare the effects of folic acid usual doses (400 mcg and 1.000 mcg) on the MN frequency of buccal mucosa in active smokers. It is a clinical trial conducted in November 2018 in the Laboratory of the Faculty of Medicine, Universitas Islam Bandung of 53 active smokers who divided into two treatment groups. Group A was administered by 400 mcg and group B 1,000 mcg folic acid supplementation within three weeks. The buccal mucosa smear stained with hematoxylin-eosin (HE) and observed through a light microscope with 100× and 400× magnification. Data were analyzed by the Wilcoxon test statistically. The results showed that there was a significant decrease (p=0.00) in MN frequency in folic acid supplementation for three weeks, namely group A=6.39±3.92 and group B=6.93±5.82 in pre-supplementation, and group A=3.80±2.66 and group B=3.31±2.71 post-supplementation of folic acid. Giving a dose of 400 mcg and 1,000 mcg for three weeks did not provide significant results (p=0.94) with Kruskal-Wallis test. In conclusion, administration of folic acid at usual dose give results to a decrease in the buccal mucosa MN frequency in active smokers. ASAM FOLAT DOSIS LAZIM MENURUNKAN FREKUENSI MIKRONUKLEUS MUKOSA BUKAL PADA PEROKOKAsap rokok mengandung senyawa kimia toksik yang memicu ketidakstabilan DNA. Deteksi genotoksik awal  rongga mulut ditandai dengan kemunculan mikronukleus (MN) pada mukosa bukal. Folat diperlukan dalam menjaga kestabilan DNA. Penelitian ini bertujuan mengetahui efek asam folat dosis lazim (400 mcg dan 1.000 mcg) terhadap frekuensi MN mukosa bukal pada perokok aktif. Penelitian ini merupakan uji klinis yang dilakukan pada bulan November 2018 di Laboratorium Fakultas Kedokteran, Universitas Islam Bandung terhadap 53 perokok aktif yang dibagi ke dalam dua kelompok perlakuan. Kelompok A mendapatkan suplementasi asam folat 400 mcg dan kelompok B mendapatkan suplementasi asam folat 1.000 mcg selama tiga pekan. Apus mukosa bukal diwarnai dengan hematoxylin-eosin (HE) dan diamati melalui mikroskop cahaya dengan pembesaran 100× dan 400x. Data dianalisis dengan uji Wilcoxon secara statistik. Hasil penelitian menunjukkan bahwa terdapat penurunan frekuensi MN yang signifikan (p=0.00) terhadap suplementasi asam folat selama tiga minggu, yaitu kelompok A=3,80±2,66 dan kelompok B=3,31±2,71 pada pre-suplementasi, serta kelompok A=6,39±3,92 dan kelompok B=6,93±5,82 pascasuplementasi asam folat. Pemberian dosis 400 mcg dan 1.000 mcg selama tiga minggu tidak memberikan hasil yang bermakna (p=0,94) berdasar atas Uji Kruskal-Wallis. Simpulan, pemberian asam folat dosis lazim memberikan hasil baik terhadap penurunan frekuensi MN mukosa bukal pada perokok aktif.