Ikhsan Khasani
Loka Riset Pemuliaan dan Teknologi Budidaya Perikanan Air Tawar Sukamandi

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KERAGAMAN DAN KORELASI KANDUNGAN ALBUMIN DENGAN KARAKTER PERTUMBUHAN PADA TIGA POPULASI IKAN GABUS (CHANA STRIATA) Khasani, Ikhsan; Astuti, Dessy Nurul
Jurnal Riset Akuakultur Vol 15, No 1 (2020): Maret, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.15.1.2020.%p

Abstract

Albumin ikan gabus merupakan bahan farmasi bernilai tinggi, banyak dimanfaatkan untuk mengobati pasien pascaoperasi dan luka bakar. Penelitian ini bertujuan untuk menganalisis keragaman dan korelasi kandungan albumin terhadap karakter pertumbuhan pada tiga populasi ikan gabus (Chana striata) dari Sumatera (SM), Jawa (JW) dan Kalimantan (KL). Tahap awal dari penelitian ini adalah pemeliharaan larva-benih, karakterisasi dan analisis kandungan albumin ikan gabus dari tiga populasi. Kandungan albumin pada sampel daging (20 ekor ikan per populasi) dianalisis menggunakan reagen bromocresol green dan diukur dengan spektrofotometer pada 578 nm. Benih ikan gabus dari populasi SM, JW, dan KL dengan ukuran panjang total 28,9 ± 5,7 mm dan bobot badan 0,27 ± 0,09 g dari 12 famili, terdiri dari 4 famili untuk setiap populasi dipelihara secara acak dalam 12 buah kolam beton ukuran 25 m2 selama 75 hari. Kandungan albumin rata-rata ketiga populasi tidak berbeda nyata (P>0,05), yaitu 2,6 ± 0,4 g dL-1 (KL), 2,4 ± 0,3 g dL-1 (SM) dan 2,2 ± 0,4 g dL-1 (JV); dengan koefisien keragaman sebesar 12,60% (SM), 18,13% (JT) dan 17,0% (KL). Nilai korelasi antara panjang total, panjang standar, dan bobot badan dengan kandungan albumin tergolong rendah hingga sedang, yaitu secara berurutan sebesar -0,05; -0,03 dan -0,43 (SM), 0,42; 0,475 dan 0,34 (JW) dan -0,28; -0,35 dan -0,275 (KL). Hasil analisis tersebut menunjukkan bahwa keragaman kandungan albumin tidak berbeda antar populasi, sehingga pendekatan seleksi kurang efektif. Nilai korelasi antara karakter pertumbuhan terhadap kandungan albumin pada ikan gabus tergolong rendah. The snakehead fish (SHF) albumin is a highly valued pharmaceutical material widely used to treat postsurgery wounds and skin burns. This study?s purpose was to analyze the coefficient of variance and correlation of albumin trait to growth characters of three SHF populations: Sumatera (SM), Java (JV) and Kalimantan (KL). The first step of this study consisted of larval and seed rearing of three SHF populations followed up by characterization and analysis of their albumin content. The albumin contents of the SHF meat (20 fish for each population) were analyzed using bromocresol green reagent and measured using a spectrophotometer at 578 nm. The SHF seeds (28.9 ± 5.7 mm of total length and 0.27 ± 0.09 g of body weight) from 12 families consisted of four families for each population were reared in 12 concrete ponds measuring 25 m2 for 75 days. The averages of albumin content of the three populations were 2.6 ± 0.4 g dL-1 (KL) 2.4 ± 0.3 g dL-1 (SM) and 2.2 ± 0.4 g dL-1 (JV) and considered not significantly different (P> 0.05) with coefficients of variance of 12.60% (SM), 18.13% (JV) and 17.0% (KL). The correlation values of total length (TL), standard length (SL) and body weight (BW) with albumin content of the SHF were -0.05, -0.03, and -0.43 (SM); 0.42, 0.475, and 0.34 (JV); and -0.28, -0.35, dan -0.275 (KL) and classified as low to moderate. The results suggested that the variances of albumin content among the populations were not significantly different and the individual selection method was not effective.  
UTILIZATION OF BIOFLOCS FROM NURSERY FOR FRESHWATER PRAWN (Macrobrachium rosenbergii) LARVAE REARING Sopian, Asep; Khasani, Ikhsan; A, Fajar
Widyariset Vol 16, No 2 (2013): Widyariset
Publisher : LIPI-Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.664 KB)

Abstract

The Bioflocs technology development in the aquaculture industry is very prospective due to many inheren benefits, especially for water quality control and nutrition substitution. This study aims to determine the effect of media bioflocs nursery for freshwater prawn (Macrobrachium rosenbergii) larvae rearing. The experiment design with completely randomized design was used in the study with 3 treatments and 6 replications. The treatment used were: A. without bioflocs addition (control), B. the addition of 5 ml bioflocs, and C. the addition of 10 ml bioflocs. The 1st day old larvae was used in the study and reared on a 60 l conical fiberglass at 50 ind/l of density. The main parameters were larvae development or larval stage index (LSI) and survival rate (SR). Based on the result of statistical analysis showed that the addition of bioflocs affected survival rate significantly (p<0.05) and the best survival values generated by the addition biofloc treatment as much as 5 ml.
Pengaruh Vitamin C Terhadap Perkembangan Gonad Induk Udang Galah (Macrobrachium rosenbergii) Pamungkas, Wahyu; Khasani, Ikhsan; Dewi, Raden Roro Sri Puji Sinarmi
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.30

Abstract

The objective of the experiment was to know the effect of vitamin C on gonadal development of giant freshwater prawn (Macrobrachium rosenbergii) broodstock. Female broodstock of 30-60 g and male broodstock of 40-70 g average body weight were used in this experiment. The broodstock were fed with different dose of vitamin C in feed as 0; 0.05; 0.10; and 0.15% at 3-4% of biomass weight per day for 3 months. Gonado Somatic Index (GSI) value obtained from the diet with 0.15% of vitamin C was significantly different from others (P0.05). The supplementation of vitamin C in the diet of giant freshwater prawn broodstock is important for gonadal development.
RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii Imron, Imron; Sunandar, Dadan; Susilowati, Rini; Suprapto, Rommy; Khasani, Ikhsan
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.592 KB) | DOI: 10.15578/iaj.4.2.2009.93-100

Abstract

Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.
PRODUCTION OF FEMALE GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) THROUGH HORMONAL INDUCTION Dewi, R.R. Sri Pudji Sinarni; Khasani, Ikhsan; Sularto, Sularto; Pamungkas, Wahyu
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.427 KB) | DOI: 10.15578/iaj.1.1.2006.35-38

Abstract

The objective of this experiment was to find out the appropriate dosage for producing homogametic female of giant freshwater prawn (Macrobrachium rosenbergii de Man) by feminization. This experiment was carried out at Research Institute for Freshwater Fish Breeding and Aquaculture, Sukamandi. The giant freshwater prawn at PL-5 stage was treated orally by estradiol-17ß. Estradiol-17ß was given orally. The dosage levels are 0, 30, 50, and 70 mg/kg of feed. The hormonal feed was given for 30 days. Prawns were reared in aquaria for 30 days and hapas for 60 days. The parameters observed are sex ratio, survival rate, total length, and body weight. The results showed that estradiol-17ß increased production of female giant freshwater prawn. The best dosage was 70 mg/kg of feed that could produce 65.33% ± 5.64% of female. Survival rate and growth of giant freshwater prawn were not affected by estradiol-17ß (P<0.05).
PRELIMINARY STUDY OF FISH CULTURE IN ABANDONED SAND MINING POOL Gunadi, Bambang; Dharma, Lukas; Khasani, Ikhsan; Yosmaniar, Yosmaniar; Setijaningsih, Lies
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.338 KB) | DOI: 10.15578/iaj.1.2.2006.153-158

Abstract

One of main problems in freshwater aquaculture development in Indonesia, especially in Java, is unavailability of developing zone. It is important to find an underutilized area that meets for industrial scale freshwater aquaculture, i.e. sufficient water supply, wide area, and located in one area or zone. The abandoned mining (sand, tin, etc.) pools distributed along the country might be the potential area for freshwater aquaculture business. For example, there are at least 13 water pools with total surface area of 250 ha at 15 km side of Citarum River in Karawang District (West Java Province). This study was conducted to obtain preliminary data about the prospect and potency of fish culture (tilapia, clariid catfish, and ‘patin’ catfish) in abandoned sand-mining pools in Karawang District. Mini floating net cages of 1 x 1 x 1.5 m3 size were used for culturing fish, i.e. patin catfish (Pangasianodon hypophthalmus), nile tilapia (Oreochromis niloticus), and clariid catfish (Clarias gariepinus), separately. Patin catfish were stocked at a size of 2 g with a density of 300 fish per cage, tilapia were stocked at a size of 6 g with a density of 400 fish per cage, while  the clariid catfish were stocked at a size of 1.4 g with a density of 980 fish per cage. A floating commercial feed (30%—32% protein, 3%—5% fat) was used at a daily rate of 9% biomass weight at the beginning and reduced gradually to 3% at the final culture period. Observed data showed that patin catfish grew from the initial size of 2.08 g to the final size 299.59 g in 5 months, nile tilapia grew from individual initial size of 5.92 g to the final size of 247.12 g in 14 weeks, and clariid catfish grew from initial size of 1.39 g to the final size of 73.10 g in 8 weeks. These three species were technically prospective for aquaculture development in the abandoned sand-mining pools.
EFFECT OF CALCIUM SUPPLEMENTATION ON GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) MOULTING AND EGG QUALITY Khasani, Ikhsan; Ridzwan, Narita Syawalia; Jones, Clive
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.135 KB) | DOI: 10.15578/iaj.7.1.2012.29-36

Abstract

A breeding program for giant freshwater prawn has been developed in Indonesia to supply good quality prawn seed to industry. To achieve the goal of the program, optimum conditions of both environment and nutrition must be provided. Calcium is the main compound of the prawn carapace, influencing moulting processes, especially during the premating moult. The effect of calcium supplementation in the prawn feed on moulting process and egg quality of giant freshwater prawn, Macrobrachium rosenbergii, was investigated. Experimental units consisted of 300 L plastic tanks stocked with 4 adult prawns comprising 3 female and 1 male. A standard prawn feed containing 30.39% crude protein; 0.82% Ca and 0.47% P was used as the experimental diet. A complete randomized design was‘applied in the study with 5 dosage levels of dicalcium phosphate i.e. 0%, 2%, 4%, 6%, and 8% with 4 replicates. Prawns were reared over 45 days. There were significant differences (P<0.05) in premoult, moult, and egg hatching rate, while no significant differences in intermoult or fecundity were observed (P>0.05). Egg hatching rate increased liniearly from 26.5%±9.9% to 50.8%±10.3% as calcium dosage increased from 0% to 8%. The number of eggs per spawn was not significantly different (P>0.05), ranging from 40,096 to 46,131 for females weighing 30.19 to 32.94 g. The results of this study suggest that giant freshwater prawns require dietary calcium supplementation to support moulting process and egg quality when reared in soft water.
PENYAKIT EKOR PUTIH (WHITE MUSCLE DISEASE) PADA UDANG GALAH (Macrobrachium rosenbergii de Man) Khasani, Ikhsan
Media Akuakultur Vol 8, No 1 (2013): (Juni 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.964 KB) | DOI: 10.15578/ma.8.1.2013.31-37

Abstract

Salah satu keunggulan sistem budidaya udang galah yang selama ini diyakini para pembudidaya adalah belum munculnya permasalahan penyakit serius sebagaimana pada sistem budidaya udang windu dan vaname, yang disebabkan oleh infeksi virus. Akan tetapi, pada lima tahun terakhir infeksi virus mulai dilaporkan mewabah pada sistem budidaya udang galah di dunia, dan menjadi masalah yang serius. Penyakit ekor putih (white tail disease, WTD) merupakan salah satu penyakit serius pada kegiatan pembenihan udang galah, karena dapat menyebabkan kematian hingga 100% pada fase pembenihan, dan akhir-akhir ini juga telah terjadi di beberapa hatcheri di Indonesia. Pada tahun 2011, WTD telah terjadi di hatcheri Balai Pengembangan Teknologi Kelautan dan Perikanan Samas, Jogjakarta, dan disusul pada tahun 2012 di hatcheri Balai Penelitian Pemuliaan Ikan (BPPI) Sukamandi. Makalah ini merupakan gambaran mengenai virus MrNV (Macrobrachium rosenbergii Noda Virus), dampak yang ditimbulkan, serta upaya-upaya penanganan.
RESISTENSI UDANG GALAH KETURUNAN PERTAMA TERHADAP INFEKSI Vibrio harveyi Khasani, Ikhsan; Alimuddin, Alimuddin; Junior, Muhammad Zairin; Lusiastuti, Angela Mariana; Sopian, Asep
Jurnal Riset Akuakultur Vol 10, No 2 (2015): (Juni 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.527 KB) | DOI: 10.15578/jra.10.2.2015.251-260

Abstract

Kematian massal udang galah karena infeksi penyakit merupakan masalah serius pada sistem produksi benih udang galah. Penelitian ini dilakukan untuk meningkatkan resistensi benih udang galah terhadap penyakit vibriosis menggunakan metode seleksi. Larva udang galah GIMacro diinfeksi dengan bakteri patogen Vibrio harveyi untuk mendapatkan populasi benih bertahan hidup, survivor, sebagai pembentuk induk F-0, selanjutnya disebut induk terseleksi. Sub populasi larva dari populasi tersebut tidak diinfeksi dan dipelihara hingga induk sebagai populasi kontrol. Pembentukan populasi F-1 dilakukan dengan mengawinkan antar induk F-0 terseleksi. Infeksi bakteri dilakukan terhadap larva (umur 7-9 hari pascatetas) dengan metode perendaman selama 48 jam, dengan kepadatan awal bakteri V. harveyi sebesar 5 x 105 cfu/mL. Sintasan rata-rata larva dari 24 induk betina adalah sebesar 45,92%-78,50%; dengan koefisien variasi relatif tinggi, sebesar 43%, sehingga seleksi pada karakter tersebut potensial untuk dilakukan. Respons seleksi setelah satu generasi sebesar 10,4% atau peningkatan resistensi sebesar 14,8% dibandingkan kontrol. Sintasan benih F-1 (40,04±11,9%) seleksi pada fase pembenihan standar produksi relatif lebih tinggi dibandingkan benih kontrol (38,04±15,7%). Sintasan benih pada fase pendederan juga demikian, yaitu 78,0±1,7% (F-1) dan 70±4,0% (kontrol). Bobot rata-rata benih udang galah F-1 (23,73±5,40 mg) tidak berbeda nyata dengan benih kontrol (23,40±9,50 mg). Sebagai kesimpulan bahwa peningkatan resistensi udang galah terhadap infeksi penyakit vibriosis dapat dilakukan melalui seleksi berbasis uji tantang.
PRELIMINARY STUDY ON THE EFFECT OF VITAMIN C ON THE GROWTH AND SURVIVAL RATE OF THE FRESHWATER PRAWN (Macrobrachium rosenbergii) LARVAE Khasani, Ikhsan
Indonesian Aquaculture Journal Vol 3, No 1 (2008): (June 2008)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.948 KB) | DOI: 10.15578/iaj.3.1.2008.41-44

Abstract

This experiment was conducted to investigate the influence of vitamin C on growth and survival rate of freshwater prawn larvae. The treatment dosages of vitamin C were 0.0, and 0.75 mg/L. Each treatment was in triplicates and arranged in a complete randomized design. Vitamin C was given to the larval rearing media every three days. Larvae were cultured in six conical fibreglass tanks of 50 L volume installed in a fibreglass tank (2 m x 0.8 m x 0.8 m) filled with water for temperature stabilizer. Each conical tank was stocked with 6,500 larvae (nauplii). Artemia nauplii were given on D3 and egg custard as artificial feed on D9. Larval rearing was done for 25 days in constant temperature (29oC–31oC) using automatic heater. Statistical analysis showed that metamorphosis speed of larvae was significantly affected by adding of vitamin C (P<0.05), but survival rate was similar (P>0.05). Addition of vitamin C also resulted in a better growth of freshwater prawn larvae.