Khie Khiong
Faculty of Medicine, Maranatha Christian University, Jl. Prof drg. Suria Sumantri No.65, Bandung 40164, Indonesia

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Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro Risdian, Chandra; Widowati, Wahyu; Mozef, Tjandrawati; Wargaseti, Teresa Liliana; Khiong, Khie
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Various diseases including cancer and cardiovascular diseasesare induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in thecells.Piper betle L.,commonly called betel,belongs to thePiperaceae family.It originated from South and South Eas tAsia,and has been used as a traditional medicine by people theredue to its medicinal properties.In the present study, free radicalscavenging activity of ethanolic leaves extract of Piper betle L.together with its different solvent fractions(hexane,ethylacetate,butanol,and water)were evaluated by DPPH freeradical scavenging assay.The ethanolic extract and the ethylacetate fraction had shown very significant DPPH (1,1)diphenyl)2)picryl)hydrazyl) radical scavenging activitycompared to the other fractions.In DPPH free radical scavengingassay,the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.431g/mL and 11.53 1g/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content.The total phenoliccontent was found to be highest in the ethanol extract(277.68µgEGCG/mg)and the ethyl acetate fraction(559.38µgEGCG/mg).The results concluded that the ethanolic leaves extract of Piper  betle L. and its ethyl acetate fraction have a consider ably freeradical scavenging activity.
The Effect of Ethanol Extract from Mangosteen Pericarps towards Parasitemia in Plasmodium berghei-Inoculated Mice Marito, Debora R.; Tjahjani, Susy; Khiong, Khie
Journal of Medicine and Health Vol 1, No 4 (2016)
Publisher : Maranatha Christian University

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Abstract

Mangosteen pericarps contains fenolic antioxidant, such as xanthone that acts as free radical scavenging substances and preventing heme polymerization. In this research we evaluated the effect of ethanolic extract of mangosteen pericarps on the parasitemia in Plasmodium berghei-inoculated mice and compared its antimalarial activity with artemisinin monotherapy in reducing the parasitemia in Plasmodium berghei-inoculated mice. Deutschland Denken Yoken (DDY)  mice were randomly divided into 5 groups and inoculated by Plasmodium berghei and given 0.1 mL aquadest (KN), 0.1 mg of artemisinin (KP), 2.5 mg (E1), 0.5 mg (E2) and 0,1 mg (E3) of ethanolic extract from mangosteen pericarps in 0.1 mL aquadest in 3 days. The parasitemia was observed on one day before the treatment, namely on the first day and on the day after the last treatment. We found a highly significant decrease highly significant decrease of the parasitemia in each treatment group compared to the Negative Control group (p < 0.01), and the decrease of parasitemia level in E1 group is similar to the artemisinin monotherapy group (p < 0.05).  Keywords: ethanol extract of mangosteen pericarps, artemisinin, Plasmodium berghei, malaria
Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin 8 Level in Colitis Murine Model Samuel, Lukas Mulyono; AS, Francisca; Khiong, Khie
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 16, No 3 (2015): VOLUME 16, NUMBER 3, December 2015
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.99 KB) | DOI: 10.24871/1632015148-152

Abstract

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattractant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-ĸB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis.Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a = 0.05) and p  0.05.Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p < 0.001) of IL-8 level between positive control group and treatment group.Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis.
Comparison of the Effects of Three Extract Sorts of Soybean DETAM 1 on the Total Cholesterol Level in Balb-C Male Mice Hidayat, Meilinah; Khiong, Khie; Siswanto, Christina Melissa
The Indonesian Biomedical Journal Vol 4, No 3 (2012)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v4i3.177

Abstract

BACKGROUND: Hypercholesterolemia is a major risk factor for coronary heart disease. Pharmacological treatment of  hypercholesterolemia are known to have side effects, hence the need for an alternative medicine, like herbal medicine, such as soybean as an example. Research objective is to compare the effects of ethanol extract, protein extract, and ethyl acetate fraction of soybean tempeh Detam-1 on the total serum cholesterol level of male mice.METHODS: This study was a laboratory experimental study with Completely Randomized Design (CRD) method. Twenty five balb-C strain male mice were allocated into five groups, namely: the negative-control (aquabidest), the positive-control (simvastatin), ethanol extract, protein extract and ethyl acetate fraction of the soybean tempeh Detam-1 and all were fed with high cholesterol containing food for 31 days. Starting on the 18th day all groups were treated for 14 days. The observed parameter was the total serum cholesterol level. The data of the study results were analyzed with ANOVA, followed by Tukey HSD test.RESULTS: There were significant differences between treatment groups and the negative-control (p=0.004). The reduction of the total cholesterol level of soy ethanol extract was 35.11±15%, the ethyl acetate fraction of soybean tempeh 26.48±17%, and soy protein extract was 15.33±33.1%, and no significant difference was noted in the positive-control group (simvastatin). CONCLUSION: The ethanol extract of soybean seed, protein extract of soybean seed, and ethyl acetate fraction of soybean tempeh Detam-1 have the same effect as do simvastin, and ethanol extract of soybean seed is the most effective substance for lowering total serum cholesterol in Balb-C male mice.KEYWORDS: hypericholesterolemia, ethanol extract, protein extract and ethyl acetate fraction, soybean Detam-1 
Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin 8 Level in Colitis Murine Model Samuel, Lukas Mulyono; AS, Francisca; Khiong, Khie
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 16, No 3 (2015): VOLUME 16, NUMBER 3, December 2015
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.99 KB) | DOI: 10.24871/1632015148-152

Abstract

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattractant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-ĸB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis.Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a = 0.05) and p  0.05.Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p < 0.001) of IL-8 level between positive control group and treatment group.Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis.
SISTEM UBIQUITIN-PROTEASOM DAN HUBUNGANNYA DENGAN KARSINOGENESIS YANG DIINDUKSI OLEH PAPILLOMAVIRUS MANUSIA Khiong, Khie
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 2 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.194 KB)

Abstract

Certain types of human papillomaviruses (HPV) have been etiologically associated with malignant lesion, most notably with cervical cancer. The major oncoproteins of these cancerassociated viruses are encoded by the viral E6 and E7 genes and characterization of these oncoproteins has shown that both E6 and E7 exploit the ubiquitin-proteasome system to degrade the cellular proteins.
Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro Risdian, Chandra; Widowati, Wahyu; Mozef, Tjandrawati; Wargasetia, Teresa Liliana; Khiong, Khie
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp141-145

Abstract

Various diseases including cancer and cardiovascular diseases are induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in the cells. Piper betle L., commonly called betel, belongs to the Piperaceae family. It originated from South and South East Asia, and has been used as a traditional medicine by people there due to its medicinal properties. In the present study, free radical scavenging activity of ethanolic leaves extract of Piper betle L. together with its different solvent fractions (hexane, ethyl acetate, butanol, and water) were evaluated by DPPH free radical scavenging assay. The ethanolic extract and the ethyl acetate fraction had shown very significant DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity compared to the other fractions. In DPPH free radical scavenging assay, the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.43µg/mL and 11.53 µg/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content. The total phenolic content was found to be highest in the ethanol extract (277.68 μg EGCG/mg) and the ethyl acetate fraction (559.38μg EGCG/mg). The results concluded that the ethanolic leaves extract of Piper betle L. and its ethyl acetate fraction have a considerably free radical scavenging activity.Keywords: Piper betle L., Piperaceae, free radical scavenging, DPPH
The Effect of Ethanol Extract from Mangosteen Pericarps towards Parasitemia in Plasmodium berghei-Inoculated Mice Marito, Debora R.; Tjahjani, Susy; Khiong, Khie
Journal of Medicine & Health Vol 1 No 4 (2016)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.405 KB) | DOI: 10.28932/jmh.v1i4.528

Abstract

Mangosteen pericarps contains fenolic antioxidant, such as xanthone that acts as free radical scavenging substances and preventing heme polymerization. In this research we evaluated the effect of ethanolic extract of mangosteen pericarps on the parasitemia in Plasmodium berghei-inoculated mice and compared its antimalarial activity with artemisinin monotherapy in reducing the parasitemia in Plasmodium berghei-inoculated mice. Deutschland Denken Yoken (DDY)  mice were randomly divided into 5 groups and inoculated by Plasmodium berghei and given 0.1 mL aquadest (KN), 0.1 mg of artemisinin (KP), 2.5 mg (E1), 0.5 mg (E2) and 0,1 mg (E3) of ethanolic extract from mangosteen pericarps in 0.1 mL aquadest in 3 days. The parasitemia was observed on one day before the treatment, namely on the first day and on the day after the last treatment. We found a highly significant decrease highly significant decrease of the parasitemia in each treatment group compared to the Negative Control group (p &lt; 0.01), and the decrease of parasitemia level in E1 group is similar to the artemisinin monotherapy group (p &lt; 0.05).  Keywords: ethanol extract of mangosteen pericarps, artemisinin, Plasmodium berghei, malaria