Articles

INDUKSI DAN PROLIFERASI EMBRIOGENESIS SOMATIK IN VITRO PADA LIMA GENOTIPE KEDELAI Saepudin, Adam; Khumaida, Nurul; Sopandie, Didy; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.896 KB) | DOI: 10.24831/jai.v44i3.12782

Abstract

ABSTRACTSomatic embryo induction medium was reported to be genotype dependent for soybean. This study was aimed to obtain the optimum medium for embryo somatic induction and proliferation, and to regenerate somatic embryo of five soybean genotypes. Five soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study. The research was divided into four steps: (1) embryogenic callus induction of  five soybean genotypes, (2) embryogenic callus proliferation of five soybean genotypes, (3) optimation of embryo somatic induction on five soybean genotypes and (4) embryo somatic regeneration of five soybean genotypes. The induction experiment showed that based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5. Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5, and Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes. Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used, and Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype. Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages), but in regeneration medium of MS0 and media MS + sukrosa 10 g L-1 + GA3 2 mg L-1 + BAP 4 mg L-1 + Vit B5 only Tanggamus genotype was regenerated into plantlet.  Keywords: 2,4-D, NAA, somatic embryos, induction, proliferation
ANALISIS KEMIRIPAN 20 AKSESI TEMU IRENG (CURCUMA AERUGINOSA ROXB.) BERDASARKAN WARNA RIMPANG, HASIL EKSTRAK, DAN KANDUNGAN FITOKIMIA Nurcholis, Waras; Khumaida, Nurul; Syukur, Muhamad; Bintang, dan Maria
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.174 KB) | DOI: 10.24831/jai.v44i3.12762

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ABSTRACTCurcuma aeruginosa Roxb., popularly known as ?temu ireng?, is considered as a potential source of medicinal plant for pharmacological activities. However, varieties of C. aeruginosa are still limited in Indonesia so it needs more accessions for improvement and development of new varieties. Rhizome colors, phytochemical contents and extract yield from 20 promising lines of C. aeruginosa were investigated by qualitative method for rhizome colors and phytochemical contents, and maceration method using 70% ethanol for yield extract. Similarity analysis was used for cluster analysis based on rhizome colors, phytochemical contents and yield extract. Blue was the color characterization of rhizome C. aeruginosa. The extract yield for 20 promising lines of C. aeruginosa varied from 7.92 to 19.71%, with KN and BH promising lines having the lowest and highest value, respectively. All promising lines of C. aeruginosa contain saponin and triterpenoid. Based on similarity analysis, all promising lines could be divided into 3 clusters. Cluster I consisted of 14 promising lines i.e. WG, SH, KA, GD, BH, KP, NW, PW, MB, PR, PT, KN, MD, and PK. Cluster II consisted of 4 promising lines i.e. LC, CB, KL, and GK. Cluster III consisted of 2 promising lines i.e. KD and SG. Keywords: promising lines, saponin, triterpenoid
RESPON ANATOMI DAN FISIOLOGI AKAR KEDELAI TERHADAP CEKAMAN KEKERINGAN Rosawanti, Pienyani; Ghulamahdi, Munif; Khumaida, Nurul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 3 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.956 KB) | DOI: 10.24831/jai.v43i3.11243

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ABSTRACTDrought stress in crop occurs due to imbalance between demand and supply of water. Crop responses to drought stress depend on their genotypes. Drought stress causes anatomical changes and root damage. The objective of this research was to study the effect of drought stress on anatomy and physiology of soybean root. The research was conducted at Laboratory of Microtechnique, Department of Agronomy and Horticulture, Bogor Agricultural University in November 2013. A Completely Randomized Design with two factors was used in the research i.e. soybean genotypes (Wilis, PG 57-1, SC 39-1) and PEG (0, 20%) with three replications. The result showed that genotype and PEG affected the size of the cortex, stele, and xylem. PG 57-1 genotype showed the decrease of cortical thickness, diameter of stele and the diameter of xylem, on the other hand, in SC 39-1, there was an increase. Drought stress (PEG 20%) could also cause damage to the root membrane as indicated by the occurrence of lipid peroxides. Wilis and PG 57-1 were tolerant while SC 39-1 was sensitive to drought stress.Keywords:cortex, lipid peroxides, PEG, Schiff?s reagent, xylem
RADIOSENSITIVITAS DAN KERAGAMAN UBI KAYU (MANIHOT ESCULENTA CRANTZ) HASIL IRADIASI SINAR GAMMA Maharani, Sadewi; Khumaida, Nurul; Syukur, Muhamad; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 2 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.061 KB) | DOI: 10.24831/jai.v43i2.10412

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ABSTRACTHigh genetic variability is one of important determinants in successful breeding of clonally propagated crops such as cassava. Induced mutation, including mutagenesis using gamma irradiation, is one strategy to increase genetic variability. The objective of this research was to obtain information of lethal doses (LD20-LD50) from five cassava genotypes (Jame-jame, Ratim, UJ-5, Malang-4, and Adira-4), to analyze the variability, and to obtain potentially high yielding cassava mutant candidates. Cuttings of several cassava genotypes were irradiated by 0, 15, 30, 45, and 60 Gy gamma rays. The results showed that genotype Malang-4 had the lowest LD20 (7.53 Gy) and LD50 (18.47 Gy), while genotype Jame-jame had the highest LD20 (24.94 Gy) and LD50 (33.24 Gy). The highest phenotype variability was obtained in the cassava population irradiated by 15-30 Gy gamma rays. The highest phenotype variability was also determined by the source of tissue, i.e., cuttings from the middle stem resulted in the highest phenotype variability. This research found 32 high yielding potential candidate mutants.Keywords: gamma rays, LD20, LD50,mutant, mutation
INDUKSI DAN PROLIFERASI KALUS EMBRIOGENIK PADA BEBERAPA GENOTIPE KEDELAI Khumaida, Nurul; Handayani, Tri
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.999 KB) | DOI: 10.24831/jai.v38i1.1681

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The successful of gene transformation on crop plants depends on the ability of explant to develop somatic embryos. The effect of medium composition on induction and proliferation of embryonic callus was analyzed on several soybean genotypes, including low irradiance (LI) tolerant genotype (Ceneng, Pangrango C6-30-10 and C6-76-10) and two LI sensitive genotypes (Godeg and Slamet). Immature cotyledons (14 DAA) were cultured in induction medium including MSIA (MS, vitamin B5, 30% sucrose, 0.2% gelrite, 10 mg L-1 2,4-D and 10 mg L-1 NAA) and MSIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 40 mg L-1 2,4-D). Embryonic calli was subcultered one month after initiation onto proliferation medium including MSIIA (MS, vit. B5, 30% sucrose, 0.2% gelrite, 5 mg L-1 2,4-D and 5 mg L-1 NAA) and MSIIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 20 mg L-1 2,4-D). The result showed that percentage of callused explant was 76-94% and the highest initiation obtained on Pangrango genotypes. The average of calli diameter at 1 month after initiation was 0.5-1.2 cm. Calli which was obtained on MSIA medium showed yellow, tranparent, and friable, whereas calli was obtained on MSIB medium showed yellow-brown, transparent, and friable. Increasing calli diameter and structure were obtained on proliferation medium. The combination of 2,4-D and NAA on MSIA and MSIIA both are induction and proliferation medium respectively were better than MSIB and MSIIB which were contain only 2,4-D. Ceneng genotype showed best performance of somatic embryogenesis than others, and Slamet genotype showed lowest response both on callus induction and proliferation.   Keywords: immature cotyledons, embryonic callus, NAA, 2,4
KARAKTERISASI MORFOLOGI DAN SIFAT KUANTITATIF GANDUM (TRITICUM AESTIVUM L.) DI DATARAN MENENGAH Widowati, Sartika; Khumaida, Nurul; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.256 KB) | DOI: 10.24831/jai.v44i2.13485

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ABSTRACTIndonesia is one of the largest wheat importers. Suitable environmental condition for wheat needs to be studied if wheat is going to be widely cultivated in Indonesia. The adaptability of wheat grown in various climates and altitudes is one of the important aspects. The objective of this experiment was to study the quantitative and morphological character of wheat grown in middle land (540 m asl) in Bogor, West Java. The experiment was arranged in randomized complete block design with three replications. Wheat genotypes used were three national varieties (Nias, Selayar, and Dewata), four new improved varieties (Guri 3 Agritan, Guri 4 Agritan, Guri 5 Agritan, and Guri 6 Unand), and one introduced genotype (SBD). Data were collected for several quantitative variables and seventeen morphological characters based on UPOV descriptor. The result showed that wheat growth was restricted in Bogor. Genotype determined plant height, leaf number, ear length, root length, number of spikelet, harvest time, seed weight, number of tillers, and plant biomass. Based on ear length, grain weight, and plant biomass, Guri 3 Agritan had the highest production than the other genotypes.Keywords: diversity, genetic relationship, high temperature, introduced genotype, phylogenetic
TOLERANSI BEBERAPA GENOTIPE GANDUM (TRITICUM AESTIVUM L.) TERHADAP KEKERINGAN PADA STADIA PERKECAMBAHAN Firdausya, Andina Fabrini; Khumaida, Nurul; Ardie, dan Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.707 KB) | DOI: 10.24831/jai.v44i2.13484

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ABSTRACTDrought is a major abiotic stress impeding wheat production world wide. Selection of potentially drought tolerant genotypes are necessary for wheat improvement. The objective of this study was to test the tolerance level of nine wheat genotypes to drought stress at germination stage. Assesment at germination stage using osmoticum solution is an effective method for selecting tolerant genotypes to drougt stress in a short period of time. The experiment was arranged on a randomized complete block design with two factors and three replications. The first factor was wheat genotype consisted of Nias, Selayar, Dewata, H-20, Munal, SBD, SBR, S-03, and YMH. The second factor was concentration of PEG 6000 consisted of 0, 5, 10, 15, and 20%. Observation variables were germination percentage, shoot length, root length, number of root, number of leaf, seedling fresh weight, and seedling dry weight. Increasing level of PEG concentration inhibited  the growth of wheat seedling. Based on the highes R2 value on the regression analysis, relative root length can be used as selection character. Based on RD50 value of relative root length, 15% PEG was determined concentration to select drought tolerant on wheat genotypes. Nias genotype was identified as tolerant genotype, while SBD, S-03, YMH, and Munal were identified as drought sensitive genotypes.Keywords: abiotic stress, osmotic potential, Polyethylene Glycol, RD50, relative root length
INDUKSI MUTASI KALUS EMBRIOGENIK GANDUM (TRITICUM AESTIVUM L.) MELALUI IRADIASI SINAR GAMMA UNTUK TOLERANSI SUHU TINGGI Setiawan, Ryan Budi; Khumaida, Nurul; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.095 KB) | DOI: 10.24831/jai.v43i1.9589

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Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20±4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo
POLA AKUMULASI PROLIN DAN POLIAMIN BEBERAPA AKSESI TANAMAN TERUNG PADA CEKAMAN KEKERINGAN Kurniawati, Siti; Khumaida, Nurul; Ardie, Sintho Wahyuning; Hartati, N. Sri i; Sudarmonowati, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.049 KB) | DOI: 10.24831/jai.v42i2.8432

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ABSTRACT Drought stress is one of the major limiting factors for plant growth and production. Therefore, the mechanism of drought tolerance is important to be studied. Eggplant (Solanum spp.) is relatively tolerant to drought stress compared to other member of Solanaceae family. The objective of this study was to study the mechanism of drought tolerance in eggplant related to accumulation pattern of proline and polyamines. Seven eggplant accessions were drought stressed for 21 days and rewatered for the following next 7 days. Drought stress treatment significantly decreased the soil water content and reduced plant height of all accessions. Eggplant responded the drought stress by reducing stomatal density and stomatal opening to reduce transpiration rate. Drought stress also induced proline and polyamine (PA) accumulation in the leaf tissues. These accumulations started at 14-days after drought stress period when soil water content less than 20% and reached their peaks at 21 days after drought stress. The highest level of proline (134.70 ?mol g-1) and that of putrescine (20.836 ng g-1)  could  be used as indicators for eggplant drought stress condition. Keywords: physiological responses, polyamine (PA), proline, putrescine, stomata
THE EFFECT OF PICLORAM AND LIGHT ON SOMATIC EMBRYOGENESIS REGENERATION OF PINEAPPLE Roostika, Ika; Khumaida, Nurul; Mariska, Ika; Wattimena, Gustaaf Adolf
Indonesian Journal of Agricultural Science Vol 13, No 2 (2012): October 2012
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Smooth Cayenne is the largest pineapple type cultivated in Indonesia, but its vegetative planting materials for mass propagation are limited. Somatic embryogenesis is a potential method to be applied. The aim of this study was to investigate the somatic embryogenesis regeneration under the effect of picloram and light. Callus formation was induced by picloram (21, 41 and 62 μM) added with 9 μM thidiazuron. The calli were transferred onto MS or Bac medium  enriched with N-organic compounds with or without addition of 21 μM picloram under dark or light condition. The compact calli were subcultured onto MS medium supplemented with 4.65 μM kinetin, while the friable calli were  transferred onto BIG medium (modified MS + 1.1 μM benzyl adenine + 0.9 μM indole butyric acid + 0.09 μM giberelic acid) or B medium (MS + 0.018 mM benzyl adenine). The results showed that the events of somatic embryogenesis were started from cell polarization, asymmetrical division, proembryo formation as  embryogenic tissues and friable embryogenic tissues, and embryo development. The best treatment for callus induction was 21 μM picloram. The addition of 21 μM picloram on N-organic enriched medium and the use of light condition  proliferated embryogenic calli. The N-organic enriched Bac medium and light condition yielded the highest number of mature somatic embryos (17 embryos perexplant in 2 months). The B medium was better than BIG medium to develop  somatic embryos from friable embryogenic tissues. The somatic embryogenesis method presented is potential for pineapple mass propagation and artificial seedproduction.Abstrak Bahasa IndonesiaSmooth Cayenne merupakan kultivar nenas yang banyak dibudidayakan di  Indonesia, namun ketersediaan benih untuk perbanyakan massal masih terbatas. Embriogenesis somatikadalah metode yang potensial untuk produksi bibit secara massal. Tujuan penelitian adalah untuk mempelajari pengaruh pikloram dan pencahayaan terhadap regenerasi embriogenesis somatik nenas. Kalus diinduksi menggunakan pikloram (21, 41, dan 62 μM) dan penambahan thidiazuron 9 μM. Selanjutnya, kalus dipindahkan ke media MS atau Bac yang diperkaya dengansenyawa N-organik dengan atau tanpa penambahan pikloram 21 μM dalam kondisi gelap atau dengan pencahayaan. Kalus kompak disubkultur pada media MS yang mengandung kinetin 4,65 μM, sedangkan kalus remah dipindahkan ke media BIG (MS modifikasi + bensil adenin 1.1 μM + indole butyric acid 0,9 μM + giberelic acid 0,09 μM) atau media B (MS + bensil adenin 0,018 μM). Hasil penelitian  menunjukkan bahwa tahapan embriogenesis somatik diawali dengan polarisasi sel, pembelahan asimetris, pembentukan proembrio sebagai jaringan embriogenik danjaringan embriogenik remah, serta perkembangan embrio. Perlakuan terbaik untuk induksi kalus adalah pikloram 21 μM. Penambahan pikloram 21 μM pada media yang diperkaya dengan senyawa N-organik mampu meningkatkan jumlah kalusembriogenik. Media Bac yang diperkaya senyawa N-organik dan kondisi pencahayaan menghasilkan jumlah embrio somatik dewasa terbanyak (17 embrio per eksplan dalam 2 bulan). Media B lebih baik daripada media BIG untuk regenerasi embrio somatik dari jaringan embriogenik remah. Metode embriogenesis somatik yang dihasilkan dari penelitian ini berpotensi diterapkan untukperbanyakan massal dan produksi benih nenas.
Co-Authors , Kisman , Sobir , Sudarsono , Sukendah , Trikoesoemaningtyas , Witjaksono ACHMAD . ADI SETIADI AGUS PURWITO Ahmad Rifqi Fauzi Ahmad, Ahmad Ahmad Aniversari Apriana Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arrin Rosmala, Arrin Atmitri Sisharmini Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti Bintang, dan Maria Buang Abdullah Cucu Sukmana, Cucu Darda Efendi Dede J. Sudrajat Dede Jajat Sudrajat, Dede Jajat Dewi Sartiami DEWI SUKMA DIDY SOPANDIE Diny Dinarti Effendy, Jollanda Enny Sudarmonowati Firdausya, Andina Fabrini Gustaaf A Wattimena, Gustaaf A Gustaaf Adolf Wattimena I MADE ARTIKA Ika Mariska Ika Roostika Indrastuti Apri Rumanti IRDIKA MANSUR Iskandar Z. Siregar Iskandar Z. Siregar Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Karjunita, Nike Kartika Ning Tyas Kasutjianingati . Kisman Kisman Kurniawan R. Trijatmiko Kurniawan Rudi Trijatmiko, Kurniawan Rudi La Muhuria Lefin Kafindra, Lefin Lola Adres Yanti, Lola Adres MARIA BINTANG MARTIANSYAH, Irfan Muaz Adbdul Karim MUHAMAD SYUKUR Muhammad Syukur MUNIF GHULAMAHDI N. Sri i Hartati Natalini Nova Kristina Nugroho, Candra Catur Nura ,, Nura Nurul Fauziah Pienyani Rosawanti, Pienyani Putranto, Riza Arief Rahmiati, Tengku Mia Reny Herawati Rina Hapsari Wening, Rina Hapsari Riyanti Catrina H S, Riyanti ROEDHY POERWANTO Ryan Budi Setiawan, Ryan Budi Sadewi Maharani, Sadewi Saepudin, Adam Sintho Wahyuning Ardie Sintho Wahyuning Ardie, Sintho Siti Kurniawati Slamet Budijanto Slamet Susanto Subekti, Isnani SUDARSONO SUDARSONO SURYO WIYONO Syukur, dan Muhamad Tri Handayani Tri Lestari Mardiningsih, Tri Lestari Trijatmiko, dan Kurniawan Rudi Trikoesoemaningtyas, dan Ulfah J. Siregar Ulfah J. Siregar, Ulfah Wahyuning Ardie, dan Sintho Waras Nurcholis WARID, WARID Wattimena, and Gustaaf Adolf Wening Enggarini Widodo , Widodo . Widowati, Sartika Willy Bayuardi Suwarno, Willy Bayuardi Yani, Rahmi Henda Yohanes Aris Purwanto Yudiansyah Yudiansyah