Bambang Kiranadi
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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KINERJA REPRODUKSI TIKUS BUNTING AKIBAT PEMBERIAN EKSTRAK ETANOL PURWOCEN Satyaningtijas, Aryani Sismin; Maheshwari, Hera; Achmadi, Pudji; Pribadi, Wisnugroho Agung; Hapsari, Sandra; Jondriatno, Divo; Bustaman, Isdoni; Kiranadi, Bambang
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.496 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1253

Abstract

Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.
INJEKSI CLENBUTEROL MENURUNKAN LEMAK BAWAH KULIT DAN MENINGKATKAN BOBOT KARKAS KAMBING PERNAKAN ETAWAH Kiranadi, Bambang; Saka, Ketut
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Twenty growing cross breed etawah goat were treated with clenbuterol every two days. Three levels ofclenbuterol , 5, 10 and 20 ?g/kg BW were injected intramuscularly from the thigh side. The experimentwould like to see the effect of clenbuterol on the metabolism and carcase quality. It was found thatclenbuterol did not affect the gross energy, digestibility, metaboliseable energy and urinary nitrogenexcretion.Clenbuterol will increase the feed intake up to 51.49%. Althoush not affecting protein intake, itsignificantly increased the retain protein from 70.51 up to 149.37%. Subcutaneous carcase weights werereduced between 29.6 up to 51%. Data from carcase quality showed that clenbuterol increase half emptybody weight indicating that clenbuterol is affecting protein metabolism. Dose -respond curve of clenbuterolagainst half empty body weight follows the Michaelis Menten equation and solved by Lineweaver-Burks.Result indicated that the half weight maximum was 5120 gram and Km of clemnbuterol was 9.50 x 10-8M. Clenbuterol is affecting the bone weight carcase but not affecting the intermusculer carcase.
PEMBERIAN EKSTRAK ETANOL PURWOCENG PADA MASA PASCAPLASENTASI MENINGKATKAN KINERJA REPRODUKSI TIKUS BUNTING (SUPPLEMENTATION ETHANOLIC EXTRACT OF PIMPINELLA ALPINA AT POSTPLASENTATION ON PREGNANT RAT IMPROVE REPRODUCTIVE PERFORMANCE) Satyaningtijas, Aryani Sismin; Maheshwari, Hera; Achmadi, Pudji; Bustaman, Isdoni; Kiranadi, Bambang; ., Julianto; Kurnia, Meta Levi
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was carried out to observe the effect of ethanolic extract of estrogenic purwoceng given 13-21 days age of pregnancy on reproductive performance such as the weight of ovarium, uterus, placenta, andpups, correlation ratio of corpus luteum number, implantation site number, and litter size in rats. Thepregnant rats were divided in to two groups. One groups cosisted of rats treated with purwoceng (83.33 mg/kg body weight) and the other groups was used as control (no treatment). The ovary, uterus, placenta, andpups were collected and weighed on day 21 of pregnancy. The result showed that the rats given 83,33 mg/kg BW ethanol extract of purwoceng tended to increase the weight of ovarium, uterus, and pups but it didnot increase the weight of plasenta. Number of site of implantation, ratio of implantation site to numberof corpus luteum, and ratio of number of pups to implantation site were higher on rat given 83,33 mg/kgBW ethanol extract of purwoceng. Conclusion of this study is ethanol extract of Pimpinella alpina rootsgiven at postplasentation improve reproductive performance.
PENDAYAGUNAAN KALSIUM MEDIA PERAIRAN DALAM PROSES GANTI KULIT DAN KONSEKUENSINYA BAGI PERTUMBUHAN UDANG GALAH (MACROBRACHIUM ROSENBERGII DE MAN) B. Zaidy, Azam; Affandi, Ridwan; Kiranadi, Bambang; Praptokardiyo, Kardiyo; Manalu, Wasmen
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 15 No. 2 (2008): Desember 2008
Publisher : Institut Pertanian Bogor

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Abstract

ABSTRAKPenelitian ini dilakukan untuk mengkaji pendayagunaan kapur sebagai sumber kalsium dalam proses peningkatan kadar kalsium kulit dan lama waktu postmolting, serta konsekuensinya bagi pertumbuhan udang. Perlakuan dosis penambahan Ca(OH)2 sebanyak 0 mg/L, 15 mg/L, 30 mg/L, 45 mg/L, dan 60 mg/L, dengan 3 ulangan. Parameter yang diukur meliputi kadar kalsium kulit, lama waktu postmolting, tingkat konsumsi pakan, laju pertumbuhan, dan efisiensi pemanfaatan pakan. Penambahan Ca(OH)2 sebanyak 15-60 mg/L meningkatkan kadar kalsium media (25.51-35.22 mg/L) dibanding dengan kontrol (18.53 mg/L). Pengggunaan Ca(OH)2 sebanyak 0, 15, 30, 45, dan 60 mg/L mampu meningkatkan kadar kalsium kulit pada tahap postmolting 20 hari. Penggunaan Ca(OH)2 sebanyak 30 dan 45 mg/L mampu mempercepat lama waktu postmolting, selanjutnya lebih dari 45 mg/L menghambat lama waktu postmolting. Penggunaan Ca(OH)2 selama 3 siklus kulit berimplikasi lanjut pada konsumsi pakan harian, mulai meningkat pada penambahan Ca(OH)2 15 mg/L, mencapai maksimum pada penambahan Ca(OH)2 45 mg/L, dan selanjutnya menurun pada penambahan Ca(OH)2 60 mg/L. Laju pertumbuhan individu pada penambahan Ca(OH)2 0, 15, 30, 45, dan 60 mg/L adalah 0.006, 0.010, 0.010, 0.012, dan 0.009. Efisiensi pemanfaatan pakan, mencapai maksimal pada penambahan Ca(OH)2 sebanyak 15 mg/L selanjutnya menurun pada 30, 45 dan 60 mg/L. Dengan demikian penggunan Ca(OH)2 sebanyak 30 mg/L mampu mempercepat lama waktu postmolting yang berimplikasi pada peningkatan rataan konsumsi pakan harian sehingga meningkatkan laju pertumbuhan individu udang.Kata kunci: molting, kalsium, konsumsi pakan, pertumbuhan. ABSTRACTThe objectives of these present research was to study the addition of calcium in the media in order to increase the calcium content in the skin and its consequence on the growth of the giant fresh water prawn. Five treatments of different Ca(OH)2 (0, 15, 30, 45, and 60 mg/L), concentration were prepared of which each treatment consisted of three replications. The parameters measured were the concentration calcium of exoskeleton, post molting period, daily feed consumption, total feed consumption, growth rate, and feed efficiency. The addition of 15-60 mg/L has increased the concentration of the media (25.51-35.22 mg/L) compared to the control (18.53 mg/L). Duration of postmolting of the giant freshwater prawns supplemented with 0.15, 30, 45, and 60 mg/L were 17, 15, 12, 13 and 15 days, respectively. The average of daily feed consumptions was found to be higher in the group with the input of Ca(OH)2 of 15 and maximum at 45 mg/L. The growth rate in the prawn suplemented with Ca(OH)2 of 0, 15, 30, 45, and 60 mg/L were 0.006, 0.010, 0.010, 0.12, and 0.009 The feed efficiency in the prawn supplemented with 0, 15, 30, 45, and 60 mg/L were 27.00, 40.45, 30.30, 28.20, and 26.90%. The results of this experiment recomonded that supplementation of 30 mg/L Ca(OH)2 in the aquatic media improved growth rate and feed efficiency of freshwater giant prawn.Keyword: molting, calcium, food consumption, growth.
Trehalose preincubation increases mesenchymal (CD271+) stem cells post-cryopreservation viability Kusuma, Indra; Hadi, Restu S.; Kiranadi, Bambang; Boediono, Arief
Medical Journal of Indonesia Vol 25, No 3 (2016): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.376 KB) | DOI: 10.13181/mji.v25i3.1273

Abstract

Background: Dimethyl sulfoxide (Me2SO) is a common cryoprotective agent widely used in cell preservation system. Me2SO is currently known to cause epigenetic changes which are  critical in stem cells development and cellular differentiation. Therefore, it is imperative to develop cryopreservation techniques that protect cellular functions and avert Me2SO adverse effect. Trehalose was able to protect organism in extreme condition such as dehydration and cold. This study aimed to verify the protective effect of trehalose preincubation procedure in cryopreservation.Methods: The study was conducted using experimental design. Thawed mesenchymal (CD271+) stem cells from YARSI biorepository were used for the experiment. Trehalose preincubation was performed for 1 hour, internalized trehalose was confirmed by FTIR-ATR measurement. Three groups consisted of (1) cryopreserved without trehalose preincubation, (2) cryopreserved with trehalose preincubation, and (3) did not undergo cryopreservation were evaluated after 24 hours in LN2 for viability in culture. The absorbance from each group was measured at 450 nm. The analysis performed using paired student t test.Results: Viability of thawed mesenchymal (CD271+) stem cells that undergo trehalose preincubation prior cryopreservation was significantly higher (p<0.05) compared to group without trehalose preincubation. Higher viability observed between group with trehalose preincubation compared with controlled group suggests protection to trypsinization. Mesenchymal (CD271+) stem cells incubated for 1 hour in 100 mM trehalose supplemented medium  results in 15%  trehalose loading efficiency.Conclusion: These findings confirm the protective effect of trehalose preincubation in cryopreservation. Future research should be directed to elucidate the trehalose internalization mechanism and eventually the protective mechanism of trehalose in mammalian cell cryopreservation.
PENGARUH BEBAN KERJA OSMOTIK TERHADAP PERKEMBANGAN DAN KELANGSUNGAN HIDUP LARVA UDANG GALAH MACROBRACHIUM ROSENBERGII DE MAN S Syafei, Lenny; Affandi, Ridwan; Sri Saeni, Muchammad; Praptokardiyo, Kardiyo; Kiranadi, Bambang
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 13 No. 1 (2006): Juni 2006
Publisher : Institut Pertanian Bogor

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Abstract

Rendahnya derajat kelangsungan hidup adalah salah satu kendala pada pembenihan udang galah.Kendala tersebut dapat diatasi dengan pengaturan salinitas. Penelitian ini bertujuan untuk menentukan salinitas optimum untuk perkembangan larva udang galah dengan tingkat kematian yang rendah yang ditetaskan pada salinitas 6 ppt. Hari pertama hingga hari ketujuh setelah menetas, salinitas ditingkatkan secara bertahaphingga mencapai 10.2 ppt, 11.6 ppt, 13.0 ppt, dan 14.4 ppt dan selanjutnya salinitas dipertahankan tetap hingga seluruh larva tumbuh menjadi post larva. Hasil penelitian menunjukkan bahwa salinitas 13 ppt menghasilkan beban osmotic yang terendah, mempercepat waktu perkembangan larva dan derajat kelangsungan hidupyang tertinggi.Kata kunci: larva udang galah, pengaturan osmotik, pertumbuhan, kelangsungan hidup, tingkat kematian.
Perkembangan Anak Tikus (F1) Asal Induk Penerima Asam Valproat sebagai Model Diabetes Mellitus SUNARYO, HADI; MANALU, WASMEN; WINARTO, ADI; KIRANADI, BAMBANG
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Untuk melakukan penelitian dan pengembangan obat antidiabetes diperlukan hewan model yang sesuai dengan kondisi diabetes. Tujuan penelitian ini ialah untuk mengembangkan model diabetes yang sesuai kondisi patofisiologi diabetes. Induk tikus yang telah bunting dibagi dalam 2 kelompok, satu kelompok diberi asam valproat 250 mg/kg bb per oral pada kebuntingan hari ke-9 dan kelompok lain sebagai kontrol. Pada umur anak tikus (F1) 8, 16 dan 24 minggu diambil sampel darah untuk dianalisis kadar glukosa, kadar insulin dan trigliserida. Pada waktu yang sama, jaringan pankreas diambil dalam kondisi teranestesi untuk dilakukan immumunohistokimia. Hasil pengukuran kadar glukosa dan histologi pada tikus (F1) umur 8 minggu belum menunjukkan adanya perbedaan yang bermakna dibanding kontrol normal. Pada tikus (F1) umur 16 dan 24 minggu kadar glukosa darah dan konsentrasi insulin serum menunjukkan peningkatan yang signifikan, sedangkan sel yang positif insulin menurun. Dapat diambil kesimpulan pemberian asam valproat pada tikus pada hari ke-9 kebuntingan dapat menghambat fungsi sel ? pankreas. Dengan demikian dapat disimpulkan tikus (F1) yang induknya diberi asam valproat pada masa kebuntingan dapat menyebabkan sindrom metabolik yang dimulai pada umur 16 minggu dan berlanjut sampai usia tua.