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PENYELAMATAN EMBRIO Dendrobium anosmum Lindl. MELALUI KULTUR IN VITRO Dewi, Putu Yuni Astriani; Astarini, Ida Ayu; Kriswiyanti, Eniek
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (764.982 KB) | DOI: 10.24843/metamorfosa.2016.v03.i02.p10

Abstract

The aim of the research is to analyse germination percentage, effect of coconut water addition in the media to number of last stage embryos and development of final stage D. anosmum Lindl. embryo. Research was conducted at Plant Structure and Development of Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Udayana and UPT. Balai Benih Induk Tanaman Pangan dan Hortikultura Provinsi Bali from February 2015 to July 2015. Three type of media were employed, i.e. PDA, Organic, MS. Three different concentration of coconut was added to media, i.e. 0 ml/L 50 ml/L, 100 ml/L. Results showed that seed D. anosmum Lindl. on PDA, Organic and MS has not germinated yet on all media, only swollen cells observed. Seed dormancy and maturity of seed were the main factors affecting seed germination.  Addition of 100 ml / L coconut water in MS showed that media is capable to increase the formation of final stages embryos (> 32 cells). Embryo development on PDA and Organic largely showed cell stage embryos 1 to 8 cells, while MS media already showed embryo development stages of > 32 cells.
UJI VIABILITAS SERBUK SARI Hemerocallis fulva L. (Liliaceae) SETELAH PENYIMPANAN PADA WAKTU DAN SUHU YANG BERBEDA Sari Dewi Handayani, Ni Kadek; Kriswiyanti, Eniek; Astarini, Ida Ayu
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

This study aims to determine the type of Hemerocallis fulva L. pollen, pollen viability and growth after storage at 280C, 40C, -200C, -800C temperature for 0-8 weeks. The study was conducted from January - March 2010. Pollen samples were taken from UPT. Botanical Gardens Plant Conservation 'Eka Karya’ Bali. Pollen storage and observations was carried out at the Laboratory of Plant Biotechnology and Development Structure, Biology Department, Faculty of Science, Udayana University. Viability test were conducted using hanging drop media and agar media with GA3. The results showed that type of Hemerocallis fulva L. pollen were: monosulkat, Prolate spheroiodal, (54.25 X 47.47), P/E: 1.14, Zono, colpate, reticulate. Fresh pollen viability on hanging drop media was 8.33%, decreased to 4% after storage at 4°C for one week and -20°C for 5 weeks. Whereas on agar media, control pollen has viability of 3.17% and increased at -20°C to become 11.67%. Mature pollen can be observed at the level of uninukleat, binukleat, and trinukleat. The development of pollen with the media hanging drop on the control has a long reed 115.94 ?m and length increased at 4 ° C with storage duration ie 256.39 weeks to 1 ?m, whereas on an agar medium + GA3 to control reed 149.82 ?m in length and at 28 ° C, 4 ° C, -20 ° C and -80 ° C respectively 94.73 ?m; 142.1 ?m; 95.53 ?m; 118.67 ?m at 1 week storage time.
DNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND Suwardike, Putu; Rai, I Nyoman; Dwiyani, Rindang; Kriswiyanti, Eniek
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.565 KB) | DOI: 10.24843/IJBB.2019.v07.i01.p05

Abstract

Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97. At a coefficient of 0.27, accessions were divided into 2 major groups: group A and group B. Group A consisted of two accessions, namely, KRA-005 and BDG-006 (Mangifera foetida Lour.), which had a similar coefficient of 0.657. Group B was divided into twosmaller groups, namely, groups B1 and B2, at a similarity coefficient of 0.342. Group B1 consisted of 39 accessions, while group B2 consisted of 3 accessions. Accessions Madu Anggur, Gading, Sambuk Mengwi, Kakul and Pakel Sulangai were identified as having unique alleles.
UJI VIABILITAS DAN PERKEMBANGAN SERBUK SARI BUAH NAGA PUTIH (HYLOCEREUS UNDATUS (HAW.) BRITTON & ROSE), MERAH (HYLOCEREUS POLYRHIZUS (WEB.) BRITTON & ROSE) DAN SUPER MERAH (HYLOCEREUS COSTARICENSIS (WEB.) BRITTON & ROSE) SETELAH PENYIMPANAN SARI, NI KADEK YUNITA; KRISWIYANTI, ENIEK; ASTARINI, IDA AYU
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The aim of the research was to determine pollen viability, pollen tube length and pollen development of white, red and super red dragon fruit after storage at different temperatures and times. The method used to test pollen viability was hanging drop technique and to observe the development of pollen used acetolysis techniques. The results showed viability and pollen tube length of white, red and super red dragon fruit after storage at temperature of 10° C and -20° C for 1, 2 and 3 weeks decreased (66% and 25%), tended to increase (2% ) after 4 weeks. Viability and pollen tube length decreased (100%) after storage at 30° C for 4 weeks. Pollen development of white, red and super red dragon fruit after storage at 30°C, 10°C and -20°C for 1 to 4 weeks showed the majority of pollen consists of uninucleat and binucleat.
POLA REPRODUKSI PADA SALAK BALI (SA/ACCA ZA/ACCA VAR. AMBOINENSIS (BECC.) MOGEA KRISWIYANTI, ENIEK; MUKSIN, I KETUT; WATINIASIH, LUH; SUARTINI, MADE
Jurnal Biologi Udayana Vol 12 No 2 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Research on reproduction patterns of Balinese snake skin fruits (Salak Bali) has been conducted by identifying the availability of pollens on anthers of male and hermaphrodite flowers, the viability of pollens in the pistillum and the present of embryo(s) in the ovulum. Flower samples were collected from Sibetan and Muncan Villages, Karangasem regency. Sample preparations and identifications were conducted in "Stuktur dan Perkembangan Tumbuhan Laboratory" Department of Bilogy, FMIPA Udayana University. The results showed that the pollen has a single apperture, unisulcus, minuta, oblat sferoidal type. The length of polar axis was 15-24 jam (P) and equatorial diameter of 16-24 |im (E). Therefore, the P/E index was 0.99-1.0. The pollen viability before and after anthesis of hermaphrodite flowers was low (71.4% and 50.6% respectively), with very low pollen tube germinations (0.08% of before and 0.48% after anthesis). Similarly, on the anthers of male flowers, the viability of pollens before anthesis was low (75.7%) with pollen germination of 0.18%, and event lower in after anthesis flowers (28.8%) with 0.29% pollen tube germination. Squashed stigma before and after anthesis and 2 days after flowers were opened showed that there was no germination occurred. Therefore, there was no germination in the pistillum, but the embryo developed before flower anthesis, indicates that the reproduction pattern of Salak Bali occurred without fertilizations (agamospermy).
INDEKS MITOSIS UJUNG AKAR KECAMBAH CABE BESAR (Capsicum annuum L.) SETELAH PERLAKUAN SUSPENSI Trichoderma sp. Deno Raja, PetroneLa; Kriswiyanti, Eniek; Darsini, Ni Nyoman
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui indeks mitosis ujung akar kecambah cabe besar (Capsicum annuum L.) setelah perlakuan suspensi Trichoderma sp. Penelitian ini dilakukan di laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi FMIPA, Universitas Udayana dari Oktober 2013-November 2013. Metode yang digunakan adalah metode squash, biji cabe untuk kontrol direndam dalam air ± 6 jam, untuk perlakuan biji setelah direndam air, direndam lagi dalam suspensi Trichoderma sp. 10-7 selama ± 6 jam, selanjutnya dikecambahkan. Ujung akar kecambah 2 mm dipotong, difiksasi dalam larutan farmer ± 2-24 jam, dihidrolisis dalam larutan 3N HCL ± 2-5 menit dan kemudian pewarnaan dengan aceto orcein ± 5 menit. Pengamatan dilakukan dengan mikroskop binokuler, data pembelahan tiap fase mitosis dihitung (%), dicatat dan difoto, dan dianalisis dengan menggunakan uji paired T tes.Hasil penelitian menunjukkan bahwa Trichoderma sp. berpengaruh terhadap indeks mitosis sel ujung akar Capsicum annuum L.,  pada fase metafase berbeda nyata antara kontrol dan perlakuan, sedangkan pada fase profase, anafase dan telofase berbeda tidak nyata.  Pada perlakuan persentase fase profase, metafase, anafase dan telofase (77,14%; 12,96 %; 5,88 % dan 5,23 %) lebih tinggi dari kontrol (66,40 %; 5,44 %; 4,96 % dan 4,66 %).
KEANEKARAGAMAN KARAKTER TANAMAN KELAPA (Cocos nucifera L. ) YANG DIGUNAKAN SEBAGAI BAHAN UPACARA PADUDUSAN AGUNG Kriswiyanti, Eniek
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The study was aimed to identify the characteristic variations of the coconuts (Cocos nucifera L.) trees that are used as materials for the Padudusan Agung ceremony in Bali. Exploration, interview, and observation of character variations of coconuts have been conducted from coconut plantation area from each regency in Bali. The characteritations was identified based on measurements and observations of parts of plant according to “Discriptors for Coconuts”. The result shows that there was a narrow variation on the stem and leaf characters (2.81-11.83%), while high variations found on the fruit numbers in a bunche for the generative characters (42.8%). Padudusan Agung ceremony uses eleven types (cultivars) of coconuts. Those are: gadang, gading, bulan, and surya with green, yellow, white, red epicarpium colours respectively. Young fruit of Udang coconuts have red mesocarpium, sudamala has double spathas, bingin has root grown on stem, be julit has plicated primordium leaves, bojog has hush likes the color of monkey hairs, ancak has branched stem, and rangda has petiole twisted on the top of the stem.
KINSHIP ANALYSIS OF GRASS JELLY IN REGENCY OF GIANYAR, TABANAN AND BADUNG BASED ON MORPHOLOGICAL AND ANATOMICAL CHARACTERISTIC Mursafitri, Eka Budi; Kriswiyanti, Eniek; Sutara, Pande Ketut
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Grass jelly is one of the plants that has considerable potential as medicine and drinks. This study was conductedto determine how kinship plant species Grass jelly from three districts. The Exploration of Grass jelly plantsconducted in Gianyar, Tabanan and Badung, Bali province. Making preparations in the Structures Laboratory ofPlant Development (SPT) Faculty Udayana University and the Center of Veterinary (BBVet). This research wascarried out from September 2015-January 2016. This research method using the technique of embedding andfresh slices, FAA fixative (formaldehyde: glacial acetic acid: alcohol 70% = 1: 1: 9), 1% safranin staining in 70%alcohol. For the analysis of kinship qualitative and quantitative data were suspended leaf anatomical charactersto create table Taxonomy Operation Unit (OTU). The results are used OTU table into mini-tab program version14.The result showed that four species of grass jelly plants are Cyclea barbata, Stephania japonica, Stephaniacapitata and Cocculus orbiculatus. Leaf form is like shields and ellipse. Kinship four types of plant grass jellyfrom three districts are very much based on morphological and anatomical characters (level 15.64% similarity).
STUDI VARIASI UKURAN SERBUK SARI KEMBANG SEPATU (HIBISCUS ROSA-SINENSIS L.) DENGAN WARNA BUNGA BERBEDA DENNI APRIANTY, NI MADE; KRISWIYANTI, ENIEK
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The purpose of this study conducted to know variety of : long of axis polar, equatorial plane diameters, and P/E index of ten Kembang Sepatu (Hibiscus rosa-sinensis L.) with differnt colour flowers. Acetolysis method and 1 % Safranin staining used to make pollens slides, micrometri were used to measured long of polar axis and diameter of pollens. The result of this research showed : pollen structure have: 90,10 ± 3,02 ^im to 117,42 ± 1,37 l^m axis polar long, 89,66 ± 3,13 |^m to 112,92 ± 0,94 (xm equatorial plane diameter, and 0,99 to 1,04 P/E index. Based on these data is indicated that the type of the pollen are prolat sferoidal (except bud type red corolla, and white flower) polypantoporat type appertura and periporat ornamentation exine .
Karakteristik Dan Viabilitas Serbuk Sari 38 Ragam Tanaman Kamboja (Plumeria spp.) Di Bali Sanjaya, I Komang Alit Adi; Kriswiyanti, Eniek; Darmadi, Anak Agung Ketut
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (800.199 KB) | DOI: 10.24843/metamorfosa.2020.v07.i01.p06

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik dan viabilitas serbuk sari ragam tanaman kamboja (Plumeria spp.) di Bali. Pengamatan karakteristik serbuk sari dengan menggunakan metode asetolisis, sedangkan pengamatan viabilitas serbuk sari dengan metode warna 1 % anilin blue. Pengukuran dilakukan mengunakan mikrometri. Hasil pengamatan terhadap 38 ragam tanaman kamboja menunjukkan adanya variasi panjang aksis polar antara 36,48 ± 4,91 – 57,87 ± 3,89 µm, diameter bidang ekuatorial antara 30,18 ± 4,64 – 55,24 ± 5,52 µm, indeks P/E antara 0,85 ± 0,10 – 1,42 ± 0,13 serta viabilitas serbuk sari sebesar 6,67 – 42,31%. Presentase viabilitas serbuk sari tertinggi dijumpai pada ragam P. alba L. 'Snow White' sebesar 42,31 sedangkan terendah dijumpai pada ragam P. rubra L. 'Red Ajanta'. Berdasarkan indeks P/E serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok subpheroidal hingga prolat. Berdasarkan panjang aksis polar serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok media hingga magna.