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DAYA LARVASIDA EKSTRAK DAUN MUDA KEDONDONG HUTAN TERHADAP HAEMONCHUS CONTORTUS SECARA IN-VITRO (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) Wirawan, I Gusti Komang Oka; Nurcahyo, Wisnu; Prastowo, Joko; Kurniasih, Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.02 KB) | DOI: 10.19087/jveteriner.2017.18.2.283

Abstract

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).  
TINGKAT KERUSAKAN DNA SPERMATOZOA MEMENGARUHI PROFIL PROTEIN SPERMATOZOA PADA SEMEN BEKU SAPI BRAHMAN (LEVEL OF SPERMATOZOA DNA DAMAGES AFFECTS SPERMATOZOA PROTEIN PROFILES IN BRAHMAN BULLS FROZEN SEMEN) Priyanto, Langgeng; Budiyanto, Agung; Kusumawati, Asmarani; Kurniasih, Kurniasih
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.73 KB) | DOI: 10.19087/jveteriner.2018.19.4.512

Abstract

The purpose of this study was to investigate the relationship between spermatozoa DNA damages with spermatozoa protein profiles of after freezing. The rate of spermatozoa DNA damages was measured by Sperm-Bos-Halomax from two Brahman bull straw samples and the spermatozoa protein was isolated by separating the upper and lower fractions of the centrifugation results. The protein profiles were then analyzed using SDS PAGE with a 12.5% separating gel concentration. The data obtained were analyzed descriptively by comparing level of spermatozoa DNA damages with spermatozoa protein profiles of upper and lower fractions. The results of the analysis showed that in the upper fractions at 37.11% level of spermatozoa damages, one protein band (29 kDa) and at 10.66% level of DNA spermatozoa damages 9 protein bands (128 kDa, 110, 91, 55, 44, 29, 27, 25 and 20 kDa) were found, respectively. Meanwhile, in the lower fractions of frozen semen, at 37.11% level of spermatozoa DNA damages 4 protein bands (105, 82, 56 and 25 kDa), and at 10.66% level of spermatozoa DNA damages 8 protein bands (109, 95, 79, 69, 50, 44, 24 and 18 kDa) were found, respectively. It can be concluded that there are differences in the spermatozoa protein profiles between different levels of spermatozoa damages. 
PAT-2 RAPID DIAGNOSTIC TEST OF RED SEA BREAM IRIDOVIRAL DISEASE (RSIVD) IN GROUPER EPINEPHELUS SP. BASED ON SEROLOGICAL CO-AGGLUTINATION AND MOLECULAR STUDY Sulistiyono, Dwi; Amanu, Surya; Kurniasih, Kurniasih; Kristianingrum, Yuli Purwandari
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.403 KB)

Abstract

Red sea bream iridoviral disease (RSIVD) is caused by red sea bream iridovirus (RSIV), adouble stranded DNA of Icosahedral virus with a diameter of 120-240 nm [1]. RSIV is  one  of the  species  of  the Megalocytivirus,  Genus  of  the  Iridoviridae Family,  first reported to infect red sea bream (Pagrus major) fish, at Sikoku Island, Japan 1991, and since then it has been noted to cause considerable economic losses to fisheries in Singapore, Taiwan, Thailand, Korea, Philippines, Malaysia and also in Indonesia [2,3,4]. Rapid transmission with high mortality rates in fish populations infected becomes a serious threat to the aquaculture fishery business. Stained imprints or tissue sections [1], monoclonal antibody technique (MAb), Immunofluorescent Antibody Tests (IFAT) [5], Polymerase Chain Reaction (PCR) [6] Electron Microscope and Multiplex PCR [2] methods have been introduced.  Although it is very effective for detecting RSIVD in infected fish, but requires training and specialized equipment at a high cost.Co-agglutination test is a diagnostic method, used both in humans and animals in detecting bacterial or viral diseases [7], this method is fast, easy to use, and does not require special equipment. Test results from co-agglutination are easily seen macroscopically, so it is suitable if developed in RSIVD detection in the field case. This study aims to create and conduct RSIVD co-agglutination kit field tests supported by molecular studies and diagnostic analysis of the sensitivity and specificity of the accuracy and reliability of the kit. Then the test results will be compared from the pooling and individual samples.
DAYA ANTHELMINTIK INFUSA BIJI BUAH PINANG (ARECA CATECHU) TERHADAP CACING ASCARIDIA GALLI SECARA IN VITRO Mubarokah, Wida Wahidah; Nurcahyo, Wisnu; Kurniasih, Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7800.591 KB) | DOI: 10.22146/jsv.40337

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A. galli infection caused high economic losses and decreased the amount and quality of egg production. The existence of anthelmintic resistance lead to the development of research on alternative treatments for A. galli. This aim of the research was to know LC50 of Areca catechu on infestation of A. galli. In this research A. galli was taken from a chicken slaughterhouse in Terban, Yogyakarta, and was divided into 8 groups for in vitro research. Group I was treated with an 10% infusion of A. catechu; group II was treated with 12.5% infusion of A. catechu; group III was treated with a 15% infusion of A. catechu; group IV was treated with a 17.5% infusion of A. catechu; group V was treated with 20% infusion of A. catechu; group VI was treated with a 22.5% infusion of A. catechu; group VII was treated with 25% A. catechu infusion and group VIII as negative control (0.9% NaCl). A. galli mortality is recorded every one hour until worm mortality is 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test the infusion of Areca catechu against Ascaridia galli can cause death in worms with an LC 50 calculation of 21. 18%.
IN VITRO DEVELOPMENT OF ASCARIDIA GALLI EGGS INTO INFECTIVE EGGS AND LARVAE OF STADIUM 2 (L2) Mubarokah, Wida Wahidah; Kurniasih, Kurniasih; Nurcahyo, Wisnu; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.993 KB) | DOI: 10.21157/j.ked.hewan.v13i1.12978

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The study aimed at finding out the development of Ascaridia galli (A. gall) eggs that were given aerator treatment and those without aerator treatment into infective eggs and L2 through in vitro culture. Each treatments has 108,000 eggs assigned to 8 groups of 1,000; 2,000; 3,000; 4,000; 5,000; 6,000; 7,000; and 8,000 eggs, respectively with 3 repetitions. Female A. galli were collected from the small intestinal lumen of naturally infected domestic chickens. The eggs collected from the uterus of adult female A. galli were incubated in sterile aquadest at ambient temperature for 45 days (without aerator) and 25 days (with aerator) to obtain the infective eggs and the L2. The number of the infective eggs and hatched L2 were counted under stereo microscope. Data were analysed descriptively. There were 97.740 eggs (90.5%) in the groups without aerator developed into infective eggs and 77,040 eggs (71.3%) developed into the L2. Meanwhile, there were 101,847 eggs (94.3%) in the groups with the aerator developed into the infective eggs and88.722 eggs (82.15%) hatched L2. It is concluded that the eggs collected from worms uterus had high viability and the aerator application shortened the developing period of the A. galli worms.
KELANGSUNGAN HIDUP IKAN MAS (CYPRINUS CARPIO L) YANG DIBERI PAKAN EKSTRAK BAWANG PUTIH (ALLIUM SATIVUM) DAN DI INFEKSI AEROMONAS HYDROPHILA Lukistyowati, Iesje; Kurniasih, Kurniasih
Jurnal Perikanan dan Kelautan Vol 16, No 02 (2011)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.89 KB) | DOI: 10.31258/jpk.16.02.%p

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The aim of a research were to investigate the effectiveness of garlic extractmixed with feed given 30 days in encharging common carps after challen with againstAeromonas hydrophila with density of 106 cell/ml. This research conducted by usingcomplete random design with dose addition of garlic extract to feed were controlpositive, control negative, P1 (2,5%), P2 (5%), and P3 (10%). In order to fine out theactive component of garlic extraction methode was adopted using 70% ethanol. Theextracted filtrates were separated using the spectrophotometrical chromatrographymethod (GC-MS) and thin-layered chromatography. Identification of the maincomponents found in the ethanol extract of garlic revealed 13 components but the onethat served as antimicrobial was C6H10S2 CAS disulphide di 2-propenyl(CAS)/(Diallyldisulfide) with and area of 16,95%. Beside the result of thin layeredchromatography showed that garlic extact contained sapononin which is harmful toaquatic animals. Percentage of survival rate P3 (10%) after infected after withAeromonas hydrophila was different compare witn control positive. The highsurvival shown on P3 (10%) concentration with 70%, P1 60%, control negative 60%and P2 30%. Fourteen days after injection the cut cause by Aeromonas hydrophilaclose and the fish recuperated as shown by their lively movement.
THE EFFECT OF APUS BAMBOO (GIGANTOCHLOA APUS) LEAVES INFUSION TO MORTALITY RATE AND MORPHOMETRY OF HAEMONCHUS CONTORTUS ADULT WORM IN VITRO Widiarso, Budi Purwo; Nurcahyo, Wisnu; Kurniasih, Kurniasih; Prastowo, Joko
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.488 KB) | DOI: 10.21157/j.ked.hewan.v11i4.8167

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This present project was carried out to determine the effect of apus bamboo (Gigantochloaapus )leaves infusion to mortality rate and morphometry of Haemonchus contortus adult worms in vitro. The formulated tools were collected through camera, 3 ml syringe, object glass, deck glass, mortar, drip dropper, waterbath, microscope, stopwatch, counter check, oven, erlenmeyer flask, electric scale, surgical scissor, and pipette. The allocated materials used in this studywere bamboo leaves infusion, Haemonchus contortus adult worms, aquadestilata, ethanol and NaCl 0.62%. The research was firstly concernedwith making 0.1% and 1% apus bamboo leaves infusion. The samples were obtained from Haemonchus contortus collection. Adult worms were obtained directly from abomasum of naturally infected goats that were cut at Animal Slaughter House (RPH). To investigate the matter, mortality rates of adult worms at various doses and times of observation as well as differences in Haemonchus contortus morphometry were analyzed using ANOVA. The findings disclosed that apus bamboo leaves infusion demonstrated a significant difference in the mortality rate of adult worms Haemonchus contortusat various doses and times of observation. This report presented the findings of research that the best dose to increase the mortality of worms was 1% bamboo leaves infusion. Moreover, apus bamboo leaves infusion affected the morphometry of Haemonchus contortus adult worms, especially in body length, cervical papilla width, and spicules length in males, and body length, cervical papilla width, and vulvar length in females.   
The Intellectual Capital Effect on Financial Performances at Islamic Insurance Syah Aji, Rizqon Halal; Kurniasih, Kurniasih
Al-Iqtishad: Journal of Islamic Economics Vol 7, No 2: July 2015
Publisher : Faculty of Shariah and Law

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.875 KB) | DOI: 10.15408/aiq.v7i2.1696

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The research has analyzed the intellectual capital effect of the Islamic insurance company’s financial performance. Intellectual capital is quantified by VAICTM (Value Added Intellectual Capital), for efficiency components are physical capital coefficient (VACA), human capital coefficient (VAHU), and structural capital coefficient (STVA). Financial performance is quantified by ROA and RBC. Data is given from seven Islamic insurance companies during 2009-2013. Data analysis used is PLS (Partial Least Square). The result shows that intellectual capital factors had an affect the company’s financial performance. Intellectual capital relation to financial performance parameter value estimated coefficient 0,845 with t-statistic 46,771. VAICTM enables to elucidate financial performance variable 71, 6%, is by finding the R-square PERF value (financial performance) 0,716.DOI: 10.15408/aiq.v7i2.1696
HISTOPATHOLOGICAL CHANGES OF SCHISTOSOMIASIS FROM NAPU VALLEY, CENTRAL SULAWESI, 1NDONESIA Kurniasih, Kurniasih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8620

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Schistosomiasis is an endemic disease caused by the infection Schistosoma Japonicum (Trematoda), can attack human and animals surrounding Lindu lake and Napu valley. Central Sulawesi, Indonesia. Five mice were naturally infected through subcuian with 75 cercariae of Schistosoma japonicum emerged from snail, Oncomelanic hupensis lindoensis, which were found in the Napu valley, Central Sulawesi. Mice showed a weakness, anorrhexia, stomach ache,and died in the fifth, sixth and seventh week posiinfection. At autopsy, mice had ascites, yellowish nodules on the surface of lung, liver, serosa of the small intestine, and mesenterium. Histolagically, there were severe necrosis and the granolomatous inflammation of the liver, the spleen, the lung, the pancreas, and the small intestine.
MORPHOLOGY AND MORPHOMETRY OF HAEMONCHUS CONTORTUS IN GOATS IN YOGYAKARTA, INDONESIA Sambodo, Priyo; Prastowo, Joko; Indarjulianto, Sudarmanto; Kurniasih, Kurniasih
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.451 KB) | DOI: 10.21157/j.ked.hewan.v12i3.11026

Abstract

This research was carried out to determine the morphology and morphometry of Haemonchus contortus in goats. Adult female parasites were collected from abomasum of naturally infected goats and stored in lactophenol. Images of the parasite were acquired using lucida camera and measurement was performed using Axiovision LE software. Basic characters of the morphology and morphometry of Haemonchus spp. were identified by determining the value of the discriminant function of three parameters on spicule. Vulvar morphology was identified by vulvar flap shape. Data were presented in terms of mean, standard deviation, and percentage. A hundred percent of the parasites were H. contortus with discriminant function value 0.63. The size of female parasites was longer and wider (25.5-32.6 mm; 0.38-0.63 mm) than that of male ones (17.3-20.0 mm; 0.24-0.33 mm). There were only 2 varieties of vulvar flap, linguiform (81%), and knobbed (19%). There were 5 subclasses identified in the linguiform type, including linguiform A (39%), linguiform B (22%), linguiform C (17%), linguiform I (1%), and a new subclass of linguiform D (2%). Based on the the type of vulvar flap, H. contortus found in goats in Yogyakarta were different to those found in previous researches. The findings could be used in identifying the parasite species in small ruminants.