Articles

ISOLASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT DARI TANAH GAMBUT Larasati, Ella Dewani; Rukmi, MG Isworo; Kusdiyantini, Endang; Ginting, R Cinta Badia
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.652 KB) | DOI: 10.14710/bioma.20.1.1-8

Abstract

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification
IDENTIFIKASI JENIS PIGMEN DAN UJI POTENSI ANTIOKSIDAN EKSTRAK PIGMEN BAKTERI SERRATIA MARCESCENS HASIL ISOLASI DARI SEDIMEN SUMBER AIR PANAS GEDONG SONGO Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.736 KB) | DOI: 10.14710/bioma.19.2.95-103

Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%. Keywords: Serratia marcescens, prodigiosin, KLT, antioxidant
PENAMBAHAN FILTRAT TEPUNG UMBI DAHLIA (DAHLIA VARIABILIS WILLD.) SEBAGAI PREBIOTIK DALAM PEMBUATAN YOGHURT SINBIOTIK Alfaridhi, Khabib Khasan; Lunggani, Arina Tri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.421 KB) | DOI: 10.14710/bioma.15.2.64-72

Abstract

Yoghurt is a fermented milk product that can provide beneficial effects to the health of consumers. Increasing the quality of yoghurt can be made by combining the benefits of probiotic with prebiotic to produce sinbiotic yoghurt. Inulin is a prebiotic that found in many dahlia tuber (Dahlia variabilis Willd.). Pure inulin were expensive, so the alternative is using dahlia tuber flours filtrate as prebiotic ingredients. This study aimed to examine the addition of Dahlia Tuber Flours Filtrate (DTFF) as a prebiotic in sinbiotic yoghurt making using Lactic Acid Bacteria (LAB) Lactobacillus bulgaricus, Streptococcus termophilus dan Lactobacillus acidopilus as probiotic. This research using Completely Randomized Design (CRD) with 4 concentration treatments DTFF 0%, 3%, 6% and 9% with 3 replications. Observed variables consist of the number of total LAB, lactic acid level, pH and organoleptic attributes (aroma, flavor, consistency and impression). Data the number of total LAB, lactic acid level and pH values ??were analyzed by ANOVA, continued by Duncan test, while the organoleptic data was analyzed by Mann-Whitney U test with 95% significant level. The results showed that the addition of DTFF significantly affect the increasing of total LAB, lactic acid level, organoleptic assessment and decreasing pH of sinbiotic yoghurt. Sinbiotic yoghurt with 3% of DTFF concentration is most preferred by panelists and required the criteria of Indonesian National Standard (SNI) for yoghurt with enough likely aroma, sour taste/specify and enough thick texture with total LAB 2,4 x 108 CFU/ml, 1.3% lactic acid levels and 4.2. pH. Keywords : prebiotic, probiotic, inulin, dahlia tuber, sinbiotic yoghurt, lactobacillus bulgaricus, streptococcus termophilus, lactobacillus acidopilus
EKSPLORASI MIKROBA PENGHASIL ENZIM-ENZIM HIDROLITIK DI KAWASAN TAMAN NASIONAL LORE LINDU SULAWESI TENGAH Rahmiati, Rahmiati; Pujianto, Sri; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.46 KB) | DOI: 10.14710/bioma.18.2.14-19

Abstract

Lore Lindu National Park (TNLL) is an area that  flora, fauna and microbes, the diversity of microbes producing hydrolytic enzyme. explore the hydrolytic enzyme producing microbes in Indonesia. This study was aimed to obtain bacterial isolates were able to produce hydrolytic enzyme and characteristics. Isolation in the microbiology laboratory. Isolation  by a spread plate. Isolates in the selection hydrolytic enzyme producing selective media. Measurement of the activity of the enzyme with hydrolytic index. The results  were thirteen isolates clearing zone test , 2 protease enzyme bacterial isolates, 1 lipase enzyme bacterial isolates, 6 amylase enzyme bacterial isolates, while 4  cellulase enzyme bacterial isolates. Examination of Amylase enzyme activity was done using DNS method. L10T3 showed that the bacterial isolate optimum activity at pH 7 and at a temperature of 300C with an activity of 0.040 U / mL and 0.029 U / mL. Key word: Lore Lindu National Park, hydrolytic enzyme. isolation of bacteria
POTENSI TEPUNG UMBI DAHLIA DAN EKSTRAK INULIN DAHLIA SEBAGAI SUMBER KARBON DALAM PRODUKSI FRUKTOOLIGOSAKARIDA (FOS) OLEH KHAMIR KLUYVEROMYCES MARXIANUS DUCC-Y-003 Yuliana, Rida; Kusdiyantini, Endang; Izzati, Munifatul
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.155 KB) | DOI: 10.14710/bioma.16.1.39-49

Abstract

Penelitian ini bertujuan untuk mengeksplorasi manfaat umbi dahlia dalam bentuk tepung umbi dan ekstrak inulin sebagai substrat untuk memproduksi FOS. Produksi FOS berlangsung secara mikrobial enzimatis dengan bantuan khamir Kluyveromyces marxianus DUCC-Y-003. Pengkulturan khamir pada media kultur berlangsung selama 60 jam dan dilakukan pengukuran tiap 6 jam. Pengukuran tersebut meliputi pertumbuhan khamir, kadar fruktosa medium dan kadar total gula medium. Penentuan FOS dilakukan dengan cara mengukur nilai derajat polimerisasi (DP). DP merupakan hasil perbandingan antara kadar total gula dengan kadar fruktosa pada media kultur. DP FOS berkisar antara 2-10 dan FOS komersil dengan DP 3-5. Penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Perlakuan terdiri atas S1D1 (substrat tepung, dosis 1 g), S1D2 (tepung, 3 g), S1D3 (tepung, 5 g), S2D1 (ekstrak inulin, 1 g), S2D2 (ekstrak inulin, 3 g), S2D3 (ekstrak inulin, 5 g). Hasil penelitian menunjukkan bahwa pertumbuhan khamir tertinggi pada perlakuan penggunaan ekstrak inulin dengan dosis 1 gram, dan pertumbuhan terendah pada perlakuan penggunaan tepung dengan dosis 5 gram. Pada masing-masing perlakuan, menghasilkan produk FOS dengan nilai DP yang beragam selama waktu inkubasi 60 jam, hanya perlakuan S2D1 (ekstrak inulin, 1g) yang sudah menghasilkan ketiga jenis FOS komersil dalam waktu inkubasi 60 jam. Pada perlakuan S2D1, produk 1-kestosa dihasilkan saat inkubasi 42 jam, produk nystosa saat 48 jam dan produk fruktofurasylnystosa saat 60 jam. Kesimpulan hasil penelitian adalah perlakuan S2D1 paling efektif dalam menghasilkan FOS selama waktu inkubasi 60 jam, sedangkan perlakuan lain dapat menghasilkan FOS yang sama jika lama waktu inkubasi diperpanjang.   Kata kunci : inulin, inulinase, derajat polimerisasi, Kluyveromyces marxianus DUCC-Y-0003
IDENTIFIKASI KANDUNGAN SENYAWA KIMIA CUKA KAYU DARI SEKAM PADI Nugrahaini, Dian Laila; Kusdiyantini, Endang; Tarwotjo, Udi; Prianto, A. Heru
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.69 KB) | DOI: 10.14710/bioma.19.1.30-37

Abstract

Wood vinegar rice husk contains a chemical compound that can be used as a biopesticides. Wood vinegar contained compounds which estimated potential as repellent, antifeedant, antimicrobials, antioxidants, disinfectants and as preservatives. The content of chemical compounds in the wood vinegar must be identified. Identification were done by calculating the concentration of acid, phenol and an infrared spectrophotometer. Production of wood vinegar made by the pyrolysis process used pirolisator. Most of the wood vinegar obtained is evaporated using a rotary evaporator and partly extracted using the method of separating funnel. Compound of acid and phenol in wood vinegar evaporation were higher than the extraction of wood vinegar. These results were confirmed by an infrared spectrophotometer spectrum showed the functional groups of acid and phenolic compounds. Keywords: wood vinegar rice husk, acid, phenol, pyrolysis, infrared spetrofotometer
KARAKTERISASI MOLEKULER TANAMAN SAMBUNG NYAWA (GYNURA PROCUMBENS [LOUR.] MERR) BERDASARKAN 18S RRNA Christiningrum, Octavia Dewi; Budiharjo, Anto; Kusdiyantini, Endang
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sambung Nyawa (Gynura procumbens) is a traditional medicinal plant used by people as an antihyperglycemic drug, antihyperlipidemic and chemoprevention agents. Comprehensive information on this plant needs to be done, including DNA analysis, so it can complement morphological character of an organisms. This study aimed to molecularly characterize and determine the relationship between G. procumbens with other organisms using ribosomal 18S rRNA gene. PCR (Polymerase Chain Reaction) was used to amplify the 18S rRNA gene fragments G. procumbens. DNA sequences were analyzed using the BLAST program at the NCBI portal and construction of phylogenetic trees were tested using Bootstrap method 1000x. The results showed the 18S rRNA sequence of Gynura procumbens fragments was 1600 bp, and G. procumbens belonged in the family of Asteraceae.Key words : Gynura procumbens, PCR (Polymerase Chain Raction), 18S rRNA, Bootstrap method
PENGARUH KOMPOSISI MEDIUM PERENDAM TERHADAP MASA KESEGARAN BUNGA POTONG KRISAN (CHRYSANTHEMUM MORIFOLIUM R.) Arisanti, Desi; Prihastanti, Erma; Kusdiyantini, Endang
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chrysanthemum morifolium is a cut flower with high economic value, however this flower has relatively short freshness period, thus it is necessary to use soaking solution by adding shredded basil leaves extract solution and carrageenan hydrogel material in it. Hydrogel serves as the water provider and energy substrate for respiration process of chrysanthemum. The basil leaves extract works as anti-bacterial and anti-fungal. The soaking is done to extend the freshness period of the chrysanthemums. This study aims to describe the effect ofthe composition of basil leaves extract and carrageenan hydrogel material on soaking medium to the freshness period of chrysanthemum, also to study the microorganism growth on the soaking medium. This research uses Completely Randomize Design on factorial pattern with two factors. First, Hydrogel Concentration (H): without hydrogel, 500 ml of water (H0); 10 g of carrageenan hydrogel, 400 ml of basil solvent (H1); 10 g of carrageenan hydrogel, 500 ml of basil solvent (H2); 10 g of carrageenan hydrogel, 600 ml of basil solvent (H3). Second, basil leaves extract (K): without basil leaves extract, without hydrogel (K0); 125 g/l of basil leaves extract (K1); 250 g/l of basil leaves extract (K2); 375 g/l of basil leaves extract (K3). This research uses 16 treatments 3 replications. The analysis is done by applying ANOVA, followed by Duncan?s test at the significance level of 95%. The research parameter includes the blooming flowers percentage, freshness period of spray chrysanthemum, and the condition of the soaking medium. The findings show that the composition of 400 ml of  250 g/l concentration of basil leaves extract, and 10 g of carrageenan hydrogel can extend the freshness period of chrysanthemum by 12.67 days, and prove that some microorganisms grow in the soaking medium. Keywords: spray chrysanthemums, soaking medium, basil leaves extract, carrageenan hydrogel, freshness period.
ISOLASI KHAMIR FERMENTATIF DARI BATANG TANAMAN TEBU (SACCHARUM OFFICINARUM. L) DAN HASIL IDENTIFIKASINYA BERDASARKAN SEKUENS INTERNAL TRANSCRIBED SPACER Anggraini, Ika Anggraini; Ferniah, Rejeki Siti; Kusdiyantini, Endang
Berkala Bioteknologi Volume 2 Nomor 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Yeast is a single-celled fungus that acts as epiphytes, endophytes or parasites. Yeast is divided into fermentative yeast and oxidative yeast. Fermentative yeast can produce primary and secondary metabolites. The role of fermentative yeast is widely used in the food industry, health and energy, so necessary to be explore fermentative yeast from sugarcane stems. The purpose of this study was to isolate fermentative yeast from sugarcane stems and identify molecular yeast based on the Internal Transcribed Spacer sequences (ITS). Isolation of epiphytic and endophytic yeast was carried out by spread plate of water soak sugarcane and sugar cane juice. Yeast isolation using 2 media, PDA and YGP with chloramphenicol. Morphological characterization was carried out by observing macroscopic and microscopic characteristics. Biochemical characterization was carried out by carbohydrate fermentation test and 50% glucose media growth test. Selected isolates were molecularly identified using Internal Transcribed Spacer (ITS) sequences. Primers used are ITS 1 and ITS 4. Phylogenetic analysis using Neighbor Joining from MEGA-6 program. The results of isolation obtained 7 yeast isolates characterized morphologically and biochemically. The based result of morphology and biochemical characterization were found 1 selected isolate with name Ed 1B. Selected yeast isolate have characteristics are round colonies, creamy white, shiny surface, raised elevation, wavy edges, ovoid cell shape, cell diameter 4,74µm, budding, glucose fermentation and sucrose fermentation, but not for lactose and grow well of 50% glucose media. The results of the Basic Alignment Search Tools (BLAST) are Ed 1B isolates had 99% homology with Kodamaea ohmeri species. Key Words :  Sugarcane (Saccharum officinarum. L.), Yeast, Molecular Identification
PENGARUH PLASTIK PENGEMAS LOW DENSITY POLYETHYLENE (LDPE), HIGH DENSITY POLYETHYLENE (HDPE)DAN POLIPROPILEN (PP)TERHADAP PENUNDAAN KEMATANGAN BUAH TOMAT (LYCOPERSICON ESCULENTUM.MILL ) Johansyah, Afrazak; Prihastanti, Erma; Kusdiyantini, Endang
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 22, No 1 (2014): Volume XXII, NOMOR 1, MARET 2014
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.286 KB) | DOI: 10.14710/baf.v22i1.7808

Abstract

The increasing demand of tomatoes corresponds to the economic and population growth. To maintain the quality of tomatoes, an effort to holdup the tomato ripeness was done using several kinds of plastic packaging. This research was intended to assess the effects of using plastic packaging and the most effective kind of plastic packaging to extend tomato storage period. The research was done at BSFT Biology Laboratory Diponegoro University and Food Technology Laboratory Soegijapranata Catholic University.    The design used in the research was Completely Randomized Design (CRD) with some treatments using different  plastic packaging type (P 0 = control / without packaging, P1 = Low Density Polyethylene (LDPE), P2 = High Density Polyethylene (HDPE) and P3 = Polypropylene (PP), each treatment was repeated three times. The parameter observed was the percentage of weight loss, the change in color (using chromameter method); the hardness level (texture analyzer method) and total content of sugar. Data were analyzed by Analysis of Variance (ANOVA) then continued with Duncan's Multiple Range Test (DMRT) at the 95% significance level to find out the difference. The results show that the use of plastic as packaging materials is able to delay tomato ripeness and the effective type plastic-based packaging to weight loss and change color is HDPE and PP.
Co-Authors AB Susanto Afrazak Johansyah Agung Suprihadi Aisyah, Arina AN, Luthfy Anggraini, Ika Anggraini, Ika Anggraini, Ika Anggraini Anggrayeni, Yesti Tri Anto Budiharjo Anwar, Soni Nugraha Arfina, Devia Kusmawati Arfina, Devia Kusmawati Arina Tri Lunggani Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Budi, Khrisna Lazuardi Christiningrum, Octavia Dewi Desi Arisanti Dian Arif Rachman Elawati, Nunung Eni Elawati, Nunung Eni Endah Dwi Hastuti Enny Yusuf Wachidah Yuniwarti Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Fadhli, Hilmi Fadillah, Maria Sarah Faizah, Laila Nur Fatimah, Sona Ginting, R Cinta Badia Hadi Endrawati Hananto, Sigit Hapsari, Betalini Hermana, Nabila Swarna Puspa Hermin Hermin, Hermin Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Isworo Rukmi JOEDORO SOEDARSONO Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kodhatin, Syawal Nurangga Kristyaningrum, Dwi Kurniati, Yoni Anggun Endah Kurniawati, Laily Larasati, Ella Dewani Larosa, Sofyan Fauzi Lestari, T. A. Lunggani, Arina Mergypta, Deverina MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Z ainuri Muhammad Zaenuri Muhammad Zainuri Mulyaningtyas, Dwina Mumtianah, Olivia Nisa Munifatul Izzati Naufal, Adhitya Ningsih, Devi Ayu Nugraha, Soni Nugrahaini, Dian Laila Nugroho, Risky Panji Nuraini, Neny Nurhayati Nurhayati Nurmila, Ika Oktavia Palupi, Dyah Parastiti, Darnia Astari Pardede, Lasria Prastya, M Eka Pratama, Barri Pratama, Novi Alvita Prianto, A Heru Prianto, A. Heru Purwaningsih, Dani Ika Putri, Adde Lolita Putri, Choirul Rizka Rachmawati, Aniza Rahmawati, Fauziah Citra Rahmiati Rahmiati Rejeki Siti Ferniah Rida Yuliana, Rida Rully Rahadian Sabdaningsih, Aninditia Safitri, Linda Sahlan, Ahmad Qi Saniha Adini, Saniha Sari, Anisa Rachma Sarjana Parman Sarjiya, Antonius Setiawan Wicaksono, Setiawan Siregar, Mangasa Tua Pandapotan Siti Nur Jannah Sri Pujianto, Sri Sri Pujiyanto Suryaningsih, Vivi Susiana Purwantisari Tri Lunggani, Arina Tri Muji Ermayanti Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Usman, Doni Wardani, Mia Tri Wijanarka Wijanarka Wijanarka, W Yadi Suryadi Yeti Darmayati Zulfikar, Muhamad Fikri