Hermin Pancasakti Kusumaningrum
Biology Department, Faculty of Sciences and Mathematics, Diponegoro University

Published : 44 Documents
Articles

APLIKASI PAKAN KAYA KAROTENOID HASIL FUSI PROTOPLASMINTERGENERA DUNALIELLA SALINA DAN CHLORELLA VULGARIS PADA UDANG WINDU (PENAEUS MONODON F.) STADIA PL-20 DI DESA ASEMPAPAN, PATI, JAWA TENGAH Rahmawati, Novia; Zainuri, Muhammad; Kusumaningrum, Hermin Pancasakti
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (799.549 KB) | DOI: 10.14710/bioma.15.2.46-52

Abstract

Dunaliella salina and Chlorella vulgaris is a natural feed microalgae with high carotenoid content that can be increased using protoplast fusion technique. Protoplast fusion as one of the application fields of genetic engineering is a method for obtaining recombinant with the desired properties and profitable in a short time. This study aimed to see the effect of the addition of carotenoid-rich feed results from protoplast fusion recombinant D. salina and C. vulgaris on the survival rate and weight of shrimp post larvae. Mixed fusion results feed and artificial feed needed for the growth of post-larval shrimp, moulting and skin pigmentation. The results showed that the recombinant from protoplast fusion intergenera D. salina and C. vulgaris contains carotenoid pigments higher, reaching 124.6 mg / g bks from the second parent, namely D. salina reached 101.83 mg / g bks, while C. vulgaris 97.18 ug / g bks. Feed manufacturing is done by mixing pellets and 80-100x103 cells per 0.0225 g of feed. Application of feed carried on Penaeus monodon F. (tiger prawn) stadia PL-20 for a month. The results of weight measurements showed the highest prawn post larvae reached at artificial feeding plus recombinant protoplast fusion results intergenera D. salina and C. vulgaris and was able to raise the level of post-larval shrimp survival rate reached 88%.   Keywords: D. salina, C. vulgaris, Protoplast Fusion, Carotenoid, Penaeus monodon F.
APPLICATION OF AQUACULTURE NATURAL FOOD PRODUCE BY PROTOPLAST FUSION PROCESS OF DUNALIELLA SALINA AND PHAFFIA RHODOZYMA Hersugondo, Hersugondo; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 4 (2010): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1003.706 KB) | DOI: 10.14710/ik.ijms.15.4.236-242

Abstract

Recently, fishponder income tend to be static so that required an effort to accurately and efficiently one of them through aquacultures  food diversification. So far, efforts to observe the pattern of introduction of a new feed, applications and their impact on aquaculture and fish farmers income has never been measured. Diversification of feed use protoplast fusion process from D. salina and P. rhodozyma which rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of carotenoids can be combined through a process of protoplasm fusion making it cheaper, faster and efficient for diversification and development of carotenoid-rich diet. The specific objectives of this research is the development of natural food aquaculture with high carotenoid content using protoplasm fusion  from the microalgae D. salina and yeast P. rhodozyma. The early stage in this research will cultivate recombinant followed by quantitative analysis of carotenoid production compared with controls. The second stage is the food diversification with different concentrations of recombinant and comparisons with commercial food followed by cost-benefit analysis. The research results have been obtained which  most carotenoid-rich natural food resulted from recombinant fusion of D. salina and P. rhodozyma. Its application in vitro have increased body weight of shrimp comparing to artificial feed. In addition, recombinant food showing stable growth in both freshwater and salt water, can breed naturally and are safe for animal aquaculture consumption and also the environment. Key words : carotenoid, protoplast fusion, D. salina,  P. rhodozyma, recombinant Selama ini pendapatan petani tambak cenderung statis sehingga diperlukan suatu upaya secara tepat dan efisien salah satunya melalui diversifikasi pakan. Sejauh ini usaha untuk mengamati pola introduksi suatu pakan baru, aplikasi dan dampaknya terhadap budidaya dan pendapatan petani tambak belum pernah terukur. Diversifikasi pakan mengggunakan pakan kaya karotenoid alami sangat dibutuhkan dalam budidaya perikanan karena terbukti dapat meningkatkan keloloshidupan dan menambah bobot hewan budidaya. Kedua jenis karotenoid ?-karoten dan astaxantin dapat digabungkan melalui  proses fusi protoplasma sehingga lebih murah, cepat dan efisien untuk diversifikasi dan pengembangan pakan kaya karotenoid.  Tujuan khusus penelitian ini adalah pengembangan usaha budidaya  untuk  meningkatkan  pendapatan  petani  tambak  melalui  diversifikasi  pakan  akuakultur  dengan kandungan karotenoid tinggi hasil fusi protoplasma alga Dunaliella salina dan khamir Phaffia rhodozyma. Tahap awal dalam penelitian ini akan melakukan kultivasi pakan rekombinan Dunaliella salina dan Phaffia rhodozyma diikuti analisis produksi karotenoid secara kuantitatif dibandingkan dengan kontrol. Tahap kedua adalah diversifikasi pakan dengan perbedaan konsentrasi fusan dan perbandingan dengan jenis pakan lain diikuti dengan analisis untung rugi. Kata kunci : karotenoid, fusi protoplas, D. salina,  P. rhodozyma, rekombinan
PELACAKAN FRAGMEN GEN PENYANDI ENZIM ß-KETOASIL-ACP SINTASE II (KAS II) DARI MESOKARP KELAPA SAWIT (ELAEIS GUINEENSIS JACQ. L.) Chandrawijaya, Yohanes; Tajuddin, Teuku; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

        The standard of quality is one of the determining values of crude palm oil as an international trade commodity. Better standard of quality for crude palm oil is a constant demand of the market. Quality improvement can be made by increasing the contents of oleic acid in the mesocarp of  E. guinensis. Among the uses of oleic acid are as follows: anti-carcinogenic agent, anti-oxidant, source of pro-vitamin A, and source of Vitamin E. Oleic acid is a form of non-saturated fatty acid encoded by KAS II genes. The expression profiling of KAS II is achieved through isolation of total RNA by Trizol reagent, RNA purification, using DNAse RNAse free, synthesis of cDNA using Reverse Transcriptation PCR approach, and amplification of KAS II genes with Nested PCR approach. The amplification process of KAS II genes is carried out using both internal and external primers. The first step of the external primer PCR is F-KAS-1 and R-KAS-1. Internal primer of PCR in the second step is F-KAS-2 and R-KAS-2. The results of this research are fragments of KAS II genes between 1500?2000 bp. These amplicons are suitable with primers designed at the approximation of 1796 bp. Selection of three amplicons at the annealing temperatures of 54oC, 55.9oC, and 58oC shows good DNA band visualizations. Annealing at 58oC shows the best result with high intensity DNA band and no smear. Further research is needed to determine the accuracy of the amplicons through sequencing step.  Keywords: KAS II, Elaeis guineensis, annealing, Nested PCR, RT-PCR
ANALISIS FUSAN HASIL FUSI PROTOPLAS INTRASPESIES PICHIA MANSHURICA DUCC-015 (ANALYSIS OF FUSANT FROM PROTOPLAST FUSION INTRASPECIES OF PICHIA MANSHURICA DUCC-015) Roslenawati, Roslenawati; Kusumaningrum, Hermin Pancasakti; Pujiyanto, Sri
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

The intraspecies protoplast fusion of P. manshurica DUCC-015 was conducted in searching the fusant with greater inulinase production. Inulinase on Dahlia variabilis Willd tuber from Baturraden-Purwokerto showed inulinase activity 0,683 IU/mL. Inulinase is an enzyme that catalyzes the hydrolysis reaction of inulin polysaccharides into fructose and or fructooligosacarides. The aims of this research was revealing fusant from intraspecies protoplast fusion process of P. manshurica DUCC-015 followed by analyzing of their inulinase productivity and activity. The research metodology protoplast fusion process consist of lysis of cell wall, protoplast fusion and regeneration of intraspecies fusant P. manshurica DUCC-015. Analysis of fusant were done by Schiff Basic Fuchsin staining of fusant nuclei, also measuring the inulinase activity and inulinase production comparing with their parent. The inulinase activity will be analyzed by T-Test Independent Two Sample on 95% Confidence interval of the difference use Statistical Product and Service Solution programme (SPSS). The result of experiment gaining fusant with regeneration capability, ploidi diversity  of fusant, inulinase activity about 0,965 IU/mL while their parent 0,622 IU/mL and inulinase production 0,736  IU/mL comparing with 0,731 IU/mL during 42 hours incubations. The fusant indicated the increase of inulinase activity and production  compared with their parent.   Keywords: Pichia manshurica DUCC-015, fusant, inulinase    Fusi protoplas intraspesies Pichia manshurica DUCC-015 telah dilakukan untuk mencari fusan dengan produksi inulinase yang lebih tinggi. Inulinase pada umbi tanaman Dahlia variabilis Willd dari Baturraden-Purwokerto memperlihatkan aktivitas sebesar 0,683 IU/mL. Inulinase merupakan enzim yang mengkatalisis reaksi hidrolisis polisakarida inulin menjadi fruktosa dan atau fruktooligosakarida. Penelitian ini bertujuan untuk mendapatkan fusan hasil fusi protoplas intraspesies P. manshurica DUCC-015 yang memiliki aktivitas inulinase lebih tinggi. Rancangan percobaan fusi protoplas terdiri dari isolasi protoplas, fusi protoplas dan regenerasi fusan. Analisis fusan menggunakan pewarnaan  Fuchsin pada inti sel, mengukur aktivitas dan produksi inulinase fusan. Aktivitas inulinase dianalisis dengan Uji T Test Dua Sampel Independen pada taraf kepercayaan 95% menggunakan program Statistical Product and Service Solution (SPSS). Hasil penelitian menunjukkan fusi protoplas intraspesies P. manshurica     DUCC-015 menghasilkan aktivitas inulinase mencapai 0,965 IU/mL dibandingkan induk sebesar 0,622 IU/mL dan produksi inulinase 0,736  IU/mL pada inkubasi selama 42 jam. Fusan  mengindikasikan kenaikan aktivitas dan roduksi inulinasi dibandingkan induk. Kata kunci : Pichia manshurica DUCC-015, fusan, inulinase.
Optimization and Stability of Total Pigments Production of Fusan from Protoplast Fusion of Microalgae Dunaliella and Chlorella in vivo: Attempts on Production of Sustainable Aquaculture Natural Food Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
International Journal of Marine and Aquatic Resource Conservation and Co-existence 2014: IJMARCC Volume 1 Issue 1 Year 2014
Publisher : International Journal of Marine and Aquatic Resource Conservation and Co-existence

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Abstract

Diversification of feed rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of important carotenoids such as β-carotene, zeaxanthine can be combined through a process of protoplast fusion making it cheaper and faster. Crustaceans can not synthetize carotenoid de novo and they need it to provide nutrition and possibly disease resistance, pigmentation and esthetic value. Green microalgae produce carotenoids and can be manipulated easily by protoplast fusion. The research was conducted to obtain some fusants from interspesies protoplast fusion of D. salina and C. vulgaris. Interspecies protoplas fusion was carried out by protoplast isolation, protoplast fusion and protoplast regeneration. Microscopic and cell analysis will used to confirm positive regenerate protoplast. Analysis of the obtained fusants is limited to morphological description due to the complexity and variability of fusant. The stabilities of fusants obtained were examined by successive subcultures. The result revealed that the fusant from D. salina and C. vulgaris maintain their stability in growth and increased carotenoid production during four periods of subculturing. This result suggesting the potency of fusant to be used as food supplement.   Key words: Carotenoid, Protoplast fusion, Dunaliella, Chlorella
CHROMOSOMES AND MITOTIC CELL DIVISION PHASE IN ONION ROOTS AFTER 24 HOURS ACETOORCEIN SOAKING TIME Kusumaningrum, Hermin Pancasakti; Lunggani, Arina Tri; Nurhakim, Muhammad Amal
Bioma : Berkala Ilmiah Biologi Vol. 14, No. 2, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.889 KB) | DOI: 10.14710/bioma.14.2.46-48

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Onions (Allium cepa) are usually used in vitro to assess effect of chemical subtances by allowing developing roots to come into contact with substances to be tested. Acetic orcein staining of onion chromosomes has remained a standard method of preparation. However, aceto-orcein stain is corrosive and poisonous chemical substances since it containing oxidising agents such as organic peroxides, the toxic substances which are are cyanides, acid corrosives agents, and  also radioactive substances.  This research study mitotic activity in the roots of onion plants to determine the effects of soaking time of aceto orcein dye on actively dividing root cells. A series of several root tip from each bulb was harvested were soaked in 1, 3 and 24 hours on aceto-orcein stain and processed further for cytological studies by the aceto-orcein squash technique. The research  was carried out to study the effect of to mitotic index and chromosomal aberration  on  onion root. It will determine the percentage of cells that are undergoing mitosis. The squash techniques were used to observe mitosis in the tip of onion root cells during actively mitotic division cells time. Mitotic divisions occur in several phases, consist of prophase, metaphase, anaphase, telophase and interphase. Experiment were repeated six times for every soaking time. The data was analyzed by using T-Test. The result showed that various duration of soaking time significantly influenced the  reduction of mitotic index value. The lowest mitotic index  on glyphosate concentration 100 ppm i.e. 10. 73% and 7.19% for the duration  of soaking time 3 and 6 hours. The highest mitotic index on  glyphosate concentration 0 ppm i.e.  37.71% and 32.76% for the duration  of soaking time 3 and 6 hours. The result also showed that the chromosomal aberration were increased significantly. The lowest  chromosomal aberration  obtained i.e. 2.55% and 2.96% for the duration of aceto orcein soaking time 1, 3 and 24 hours. The highest chromosomal aberration obtained i.e. 21.71% and 36.26% for the duration of soaking time 1,3 and 24 hours. The type of chromosomal aberration were abnormal prophase, stickiness, bridge, abnormal anaphase, clumping chromosome, c- metaphase,  change of nucleous  shape and size. At 72h, their cytotoxic effects on the root tips showed strong growth retardation in high concentrations of all the wastewaters. Compared to the control, treatment with the wastewaters resulted in root growth inhibition with EC50 values of 35, 50 and 62% for bottling, rubber and brewery effluents respectively, and decrease in mitotic index with increasing concentration for all samples and these were statistically significant (p<0.05). Chromosomal aberrations induced in the onion root tip cells were mostly sticky chromosomes and bridges. Chromosomes with disturbed spindles and fragments were also present in appreciable amounts. Based on the EC50 values, the bottling wastewater was most toxic, followed by rubber effluent while effluents from the brewery were least toxic. The findings in this study indicate that there are toxic chemicals present in the wastewaters which are responsible for the observed genotoxic effects on the onion root tip cells. The study also reveals that the Allium test is a useful and reliable tool for the genotoxicity screening of industrial effluents which could be employed by environmental managers before these effluents are finally discharged into the environment.     Key words: chromosomes, onion roots, acetoorcein
THE GROWTH OF BOTRYOCOCCUS BRAUNII MICROALGAE AS A LIPID PRODUCER IN A MIXED MEDIUM OF COCONUT WATER AND SEAWATER Saputro, Bintoro Rudi; Kusdiyantini, Endang; Kusumaningrum, Hermin Pancasakti
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 4 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions for bioenergy. Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be substituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using hemocytometer. The results showed that 2.5% coconut water substitution exhibited the best growth rate and lipid production, i.e. the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control.
ANALISIS KANDUNGAN KADMIUM (CD) DALAM TANAMAN BAWANG MERAH DARI TEGAL Kusumaningrum, Hermin Pancasakti; Herusugondo, Herusugondo; Zainuri, Muhammad; Raharjo, Budi
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 4 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Ketergantungan pestisida pada sentra produksi bawang merah Kabupaten Tegal telah menimbulkan pencemaran logam berat kadmium (Cd) di dalam tanah dan tanaman bawang merah. Kadar logam tersebut telah melebihi ambang batas yang dapat mengganggu kesehatan manusia. Tujuan penelitian ini adalah melakukan analisis kandungan kadmium pada akar, daun dan umbi tanaman bawang merah dari Tegal. Metode penelitian dilakukan melalui analisis kandungan logam berat kadmium dalam tanaman bawang merah  dibandingkan dengan kontrol menggunakan metode spektrofotometri atom absorbansi (AAS). Hasil penelitian memperlihatkan kandungan kadmium pada umbi, daun dan akar bawang merah di Tegal pada umur 20 hari berturut-turut adalah sebagai berikut 3,4805 mg/g; 4,1374 mg/g dan 7,9175 mg/g. Kandungan kadmium pada umbi, daun dan akar bawang merah umur 50 hari atau usia panen berturut-turut adalah sebagai berikut 1,8331 mg/g; 1, 8331 mg/g dan 3,5323 mg/g. Hasil penelitian yang diperoleh menunjukkan konsentrasi kadmium yang melampaui batas ambang aman bagi makanan, kesehatan dan lingkungan.    Pesticide dependence on onion production centers Tegal has caused heavy metal pollution of cadmium (Cd) in the soil and plant onions. This metal content exceeds the threshold that can impair human health. The purpose of this study was to analyze lead content in root, leaves and bulb of red onion from Tegal. Research methods to analyze the content of heavy metals in the soil compared to the control using atomic absorbance spectrophotometry (AAS). The research results showed lead content in roots, leaves and roots of onion at the age of 20 days in a row is as follows 3,4805 mg/g; 4,1374 mg/g and 7,9175 mg/g. The results obtained shows that cadmium concentrations exceed safe thresholds for food, health and the environment.
PERTUMBUHAN JUVENIL IKAN KERAPU MACAN (EPINEPHELUS FUSCOGUTTATUS) YANG DIPELIHARA DENGAN PADAT PENEBARAN BERBEDA Endrawati, Hadi Ajie; Zaenuri, Muhammad Ajie; Kusdiyantini, Endang Pancasakti; Kusumaningrum, Hermin Pancasakti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 3 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.667 KB) | DOI: 10.14710/ik.ijms.13.3.133-138

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Penelitian ini bertujuan untuk mengetahui pertumbuhan juvenil ikan kerapu macan (Epinephelus fuscoguttatus) yang dipelihara dengan padat penebaran yang berbeda. Penelitian ini dilaksanakan dari April hingga Mei 2006 di Laboratorium Biologi Oseanografi UNDIP. Percobaan dilakukan di akuarium berukuran 40 x 40 x 60 cm3, dengan media air laut 10 liter. Perlakuan yang diterapkan adalah ikan kerapu macan (E. fuscoguttatus dengan tingkat kepadatan 5, 10 dan 15 ekor per aquarium. Copepoda diberikan sebagai pakan alami dengankepadatan 50 ind /L air media. Pemeliharaan dilakukan selama 4 minggu. Hasil penelitian menunjukkan bahwa semakin tinggi padat penebaran, pertambahan bobot dan panjang yangdicapai ikan kerapu macan (Epinephelus fuscoguttatus ) semakin kecil yaitu berturut-turut 3.67± 0.17; 3.21 ± 0.06 dan 2.16 ± 0.22 gram dan 0.63 ± 0.1 ; 0.55 ± 0.017 dan 0.5 ± 0.05 cm masing-masing untuk penebaran 5, 10 dan 15 ekor per wadah. Hal ini menunjukkan adanya persaingan dan kanibalisme.Kata kunci: larva, kerapu macan, Epinephelus fuscoguttatus, padat penebaran, pertumbuhan
APLIKASI PAKAN ALAMI KAYA KAROTENOID UNTUK POST LARVAE PENAEUS MONODON FAB. (APPLICATIONS RICH NATURAL CAROTENOIDS FEED FOR POST LARVAE OF PENAEUS MONODON FAB.) Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 3 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.161 KB) | DOI: 10.14710/ik.ijms.18.3.143-149

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Udang windu (Penaeus monodon Fab.) merupakan komoditas unggulan Indonesia sedangkan ketersediaan benih udang windu mengalami penurunan mencapai 50% disebabkan serangan berbagai penyakit dan keterbatasan pakan. Aplikasi pakan rekombinan hasil fusi protoplas yang kaya karotenoid telah terbukti meningkatkan bobot dan kelulushidupan larva udang windu skala laboratorium.  Teknik fusi protoplas telah terbukti mampu menghasilkan rekombinan yang memiliki gabungan karotenoid dari berbagai alga sehingga jumlah karotenoidnya semakin meningkat. Dunaliella menghasilkan karotenoid ?-karoten dan zeaxantin dalam jumlah besar sedangkan Chlorella menghasilkan lutein, ?-karoten dan astaxanthin. Tujuan khusus penelitian ini adalah mengaplikasikan pakan alami hasil fusi protoplas kedua jenis mikroalga pada larva udang windu di Balai Budidaya Air Payau Jepara untuk meningkatkan ketahanan terhadap serangan penyakit.  Penelitian ini melakukan kultivasi induk dan rekombinan diikuti analisis pigmen karotenoid dan aplikasi pakan rekombinan secara in vitro. Aplikasinya pada skala in vitro telah meningkatkan berat badan udang dan kelulushidupannya dibandingkan pakan buatan dan pakan alami saja. Selain itu pertumbuhan pakan rekombinan stabil baik di air tawar maupun air asin, mampu berkembangbiak secara alami dan konsumsinya aman bagi hewan budidaya dan lingkungan. Kata kunci: karotenoid, fusi protoplas , Dunaliella salina, Chlorella vulgaris,   Tiger shrimp (Penaeus monodon Fab.) is one of the main commodities of aquaculture products in Indonesia. The availability of Tiger shrimp larvae has decreased up to 50% due to the attack of various diseases and feed limitation. Application of feed from recombinant of protoplast fusion rich in carotenoids have been shown to increase weight and survival rate of tiger shrimp larvae on laboratory scale. Protoplast fusion technique has been shown to produce a recombinant that has combined various carotenoids from algae thus increasing their catotenoids number. Dunaliella produce carotenoids ? - carotene and zeaxantin in large numbers while Chlorella produce lutein, ? - carotene and astaxanthin. The specific objective of this research is to apply natural food of protoplast fusion recombinant from both of microalgae to Tiger shrimp larvae in Brackish Water Aquaculture Center Jepara to improve resistance to disease. This study conducted cultivation of parent and recombinant followed by carotenoid pigment analysis and application of recombinant feed in vitro. Application on a in vitro scale have increased body weight and shrimp survival compared to artificial food and natural food. Additionally feed recombinant growth were stable in both freshwater and saltwater. They also able to breed naturally and safe for animal consumption and environmental. Keywords: carotenoid, protoplast fusion, Dunaliella salina, Chlorella vulgaris