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TINGKAT KERUSAKAN DNA SPERMATOZOA MEMENGARUHI PROFIL PROTEIN SPERMATOZOA PADA SEMEN BEKU SAPI BRAHMAN (LEVEL OF SPERMATOZOA DNA DAMAGES AFFECTS SPERMATOZOA PROTEIN PROFILES IN BRAHMAN BULLS FROZEN SEMEN) Priyanto, Langgeng; Budiyanto, Agung; Kusumawati, Asmarani; Kurniasih, Kurniasih
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.4.512

Abstract

The purpose of this study was to investigate the relationship between spermatozoa DNA damages with spermatozoa protein profiles of after freezing. The rate of spermatozoa DNA damages was measured by Sperm-Bos-Halomax from two Brahman bull straw samples and the spermatozoa protein was isolated by separating the upper and lower fractions of the centrifugation results. The protein profiles were then analyzed using SDS PAGE with a 12.5% separating gel concentration. The data obtained were analyzed descriptively by comparing level of spermatozoa DNA damages with spermatozoa protein profiles of upper and lower fractions. The results of the analysis showed that in the upper fractions at 37.11% level of spermatozoa damages, one protein band (29 kDa) and at 10.66% level of DNA spermatozoa damages 9 protein bands (128 kDa, 110, 91, 55, 44, 29, 27, 25 and 20 kDa) were found, respectively. Meanwhile, in the lower fractions of frozen semen, at 37.11% level of spermatozoa DNA damages 4 protein bands (105, 82, 56 and 25 kDa), and at 10.66% level of spermatozoa DNA damages 8 protein bands (109, 95, 79, 69, 50, 44, 24 and 18 kDa) were found, respectively. It can be concluded that there are differences in the spermatozoa protein profiles between different levels of spermatozoa damages. 
CLONING OF THE GENE ENCODING SAGI OF LOCAL ISOLATE TOXOPLASMA GONDII IN ESCHERICHIA COLI DH5A Hartati, Sri; Widada, Sri; ., Sumartono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3103.953 KB) | DOI: 10.22146/jsv.503

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KANDIDAT PROBE DNA DARI URUTAN REPETITIF R529 UNTUK DETEKSI TOXOPLASMA GONDII Hartati, Sri; Kusumawati, Asmarani; Widodo, Harto; Priyo Widodo, Dwi
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2631

Abstract

Penelitian ini bertujuan merancang kandidat probe dari urutan 529 bp (R529) yang terdapat 200-300 kopi dalam genom Toxoplasma gondii (T. gondii). Untuk menguji spesifitas rancangan probe digunakan perangkat lunak basic local alignment search tool (BLASTN) dari national center for biotechnology information (NCBI) dan Geniuous. Probe DNA sebesar 237 bp dilabel yang dengan digoksigenin disintesis dengan polymerase chain reaction (PCR). Kandidat probe yang dihasilkan berpotensi untuk digunakan lebih lanjut dalam deteksi keberadaan asam nukleat T. gondii pada berbagai teknik hibridisasi.
OVULATORY FOLLICULAR DYNAMICS AFTER ESTRUS SYNCHRONIZATION USING PROSTAGLANDIN F2A IN DAIRY COWS Putro, Prabowo Purwono; Kusumawati, Asmarani
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.965 KB) | DOI: 10.22146/jsv.5419

Abstract

The study aimed to follow development of ovulatory follicular dynamics as well as plasma progesterone profile after estrus synchronization using PGF2? and GnRH.?   A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were used in the present study.     Treatment 1, given intramuscular injection of PGF2? 25 mg (PGF2?), treatment 2 PGF2? 25 mg and GnRH 250 ?g 2 days later (PGF2?-GnRH), and treatment 3 with GnRH 250 ?g (7 days prior to injection of PGF2?), PGF2? 25 mg and GnRH 250 ?g (2 days after injection of PGF2???)  (GnRH-PGF2a-GnRH) (the Ovsynch method).   Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz tranducer was performed everyday for 12 days to follow ovulatory follicular and luteal dynamics.   Blood plasma was taken every day for progesterone determination using EIA technique.   Data of follicular, luteal development and progesterone levels were tested using analysis of variance and correlation analysis.   The animals showed estrus within 70.70 + 01.90 hours following PGF2? injection.   Prostaglandin F2? induced corpus luteum regression, decreased  in progesterone plasma levels, followed by ovulatory follicular development and eventually underwent ovulation.   Administration of first GnRH increased corpus luteum size, enhanced its regression and decreased plasma progesterone levels, while  the second administration induce  better ovulatory follicular development.   Rate of the corpus luteum regression, progesterone decrease and ovulatory follicular development following PGF2? injection for respective treatments 1, 2 and 3 were 2.53 + 0.24a, 2.73 + 0.36a and 3.53 + 0.28b mm/day; 1.39 + 0.14a,  1.35 + 0.18a dan 1.57 + 0.12b ng/ml/day; and 1.33 + 0.15a,  1.63 + 0.19b and 1.67 + 0.23b mm/day, respectively (P < 0.05).   It can be concluded that PGF2? induced corpus luteum regression, decreased in  progesterone plasma levels and ovulatory follicular development.   Addition of GnRH increased corpus luteum size and plasma progesterone levels,  after PGF2? injection corpus luteum regression and progesterone decrease became more prominent, while ovulatory folliculkar development occurred much better.   . 
KOMBINASI ONE-STEP REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) DAN NUCLEIC ACID LATERAL FLOW (NALF) SEBAGAI METODE DETEKSI GEN ENV-TM VIRUS JEMBRANA STRAIN TABANAN 1987 Kusumawati, Asmarani; Fatimah, Fatimah
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8202.514 KB) | DOI: 10.22146/jsv.41146

Abstract

Jembrana disease is an acute viral disease  in Bali cattle that have short incubation period, high mortality and morbidity rate. Early diagnostic methods are needed  to prevent the further spreading of this disease. In this study, we combined One-step RT-PCR  and  NALF  methods to detect env-tm gene of Jembrana virus Tabanan 1987 strain. Viral RNA isolated from a spleen of the infected cattle was used as the template. One-step RT-PCR procedure was performed using oligoprobes  labeled digoxigenin and  right primer sequence that were designed using the primer3plus program based  on the conserves region of env-tm gene from NCBI database. The product of one-step RT-PCR was tested using NALF  method  instead of  electrophoresis. Positive result was shown by the appearance of dark lines on the test line of digoxigenin in NALF dipstick device.
PELACAKAN GEN ENV-TM VIRUS PENYAKIT JEMBRANA GALUR TABANAN 1995 DENGAN METODE NUCLEIC ACID SEQUENCE BASED AMPLIFICATON Kusumawati, Asmarani; Ratnawati, Atik; Wulandari, Ida Arlita; Hartati, Sri; Untari, Tri
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
INTRA-UTERIN ADMINISTRATION OF PROSTAGLANDIN FOR INDUCTION IN CROSS BREEDING GOATS Gustari, Sri; Kusumawati, Asmarani; Subagyo, Slamet; Putro, Prabowo P
Jurnal Sain Veteriner Vol 15, No 1&2 (1996)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8622

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Productivity and reproductivity of cross breeding goats is low. It is mainly caused by hormone deficiency and difficulties in estrus detection. The research studied the effect of intrauterine administration of prostagiandin on estrus induction and on number of pregnancies.Sixteen does were divided randomly into 2 groups. Group i were injected with prostagiandin intramuscularly. Group li were given prostagiandin by intrauterine administration. In both groups, prostagiandin were given twice with interval 11 days apart. The does which coming into estrus were mated naturally.There was a significantly different in the time of estrus between group I and group II (40.25 ± 4.94 and 80.8 ± 18.35 hours). On the other hand, there was no difference in estrus induction between those two groups (Group I : 87.5% and Group II : 62.5 %). All the does which were mated naturally were pregnant (100%). Although time to estrus after intrauterine administration of prostagfandin was longer than that of intramuscular ones, but it was a cheaper method.
DETEKSI BRUCELOSIS PADA SUSU SAPI DENGAN UJI POLYMERASE CHAIN REACTION (PCR) M. Noor, Susan; Sudharmono, Pratiwi; Kusumawati, Asmarani; Karuniawati, Anis
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2795

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Penelitian ini bertujuan mendeteksi brucelosis pada sampel susu sapi dengan uji polymerase chain reaction (PCR) dan membandingkan tingkatsensitivitas dan spesifisitasnya dengan metode milk ring test (MRT). Sebanyak 24 sampel susu sapi yang dikoleksi secara aseptik dari lapang diuji PCR dan MRT. Hasil pengujian menunjukkan bahwa 79,17% (19/24) sampel susu positif brucelosis dengan uji PCR dan 83,33% (20/24) dengan uji MRT. Sensitivitas dan spesifisitas PCR mendeteksi brucelosis masing-masing sebesar 75 dan 100% dibandingkan dengan uji MRT.
IDENTIFIKASI BRUCELLA ABORTUS ISOLAT LOKAL DENGAN BRUCELLA ABORTUS STRAIN SPECIFIC-POLYMERASE CHAIN REACTION (IDENTIFICATION OF LOCAL ISOLATES OF BRUCELLA ABORTUS USING BRUCELLA ABORTUS STRAIN SPECIFIC-POLYMERASE CHAIN REACTION ASSAY) Noor, Susan Maphilindawati; Sudarmono, Pratiwi Pujilestari; Kusumawati, Asmarani; Karuniawati, Anis
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Brucella abortus Strain Specific-Polymerase Chain Reaction (BaSS-PCR) is a single multiplex PCRtechnique which able to identify and differentiate between Brucella abortus field strains (biovar 1, 2, and4), B. abortus vaccine strains, Brucella species, and non-Brucella species. In this study, BaSS-PCR wasapplied to identify local isolates of B. abortus in order to investigate the B. abortus strains that infectedcattle in Indonesia. Fifty local strains of B.abortus isolated from infected cattle in Java (Jakarta andBandung), South Sulawesi (Maros), East Nusa Tenggara (Kupang and Belu) were used in this study. TheDNA bands were observed by agarose gel in the presence of ethidium bromide. Identification was performedbased on the size and number of DNA products amplified by PCR from each isolates. The results showedthat the 50 isolates were of B. abortus field strains. This finding showed that the cause of bovine brucellosisin Indonesia is B. abortus field strains.
KERUSAKAN DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA MEMENGARUHI TINGKAT KEBUNTINGAN SAPI BRAHMAN (DAMAGE TO DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA AFFECTING THE LEVEL OF PREGNANCY IN BRAHMAN CATTLE) Priyanto, Langgeng; Budiyanto, Agung; Kusumawati, Asmarani; Kurniasih, Kurniasih
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.1.119

Abstract

The relationship among of sperm DNA damage in cows with pregnancy rates has not been widely studied. The purpose of this study to determine the relationship of sperm DNA damage with pregnancy rate on Brahman cows. The sperm DNA damage rate was measured by Sperm-BosHalomax® from 2 samples of male Brahman bull straw (40002 and 40885) and pregnancy rate was measured from the success rate of artificial insemination. In 14 female Brahman cows divided into two groups. One group of 7 in the artificial insemination with 40002 males with 37.11% sperm DNA damage and one in artificial insemination with 40885 with 10.65% sperm DNA damage. The data obtained were analyzed descriptively by comparing sperm DNA damage with pregnancy rate.&nbsp;The results showed that at 37.11% sperm DNA damage level was found pregnancy rate 57.11% with ultrasound on 30 day and pregnancy rate 42.80% with ultrasound to 45 day. Result of research on sperm DNA damage level of 10.66% found pregnancy rate 57.11% with ultrasound to 30 day and level pregnancy 57.11% with ultrasound 45 days. The results of this study have concluded that there is a difference in the rate of sperm DNA damage with pregnancy rate in Brahman cows. The sperm DNA damage has an effect on pregnancy rate on Brahman cows.&nbsp;&nbsp;