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GROWTH AND BLOOD PROFILE OF CORAL TROUT GROUPER PLECTROPOMUS LEOPARDUS SEEDS IN DIFFERENT SALINITIES Setiawati, Ketut Maha; Mahardika, Ketut; Alit, A. A. Ketut; Kusumawati, Daniar; Mastuti, Indah
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.413 KB) | DOI: 10.29244/jitkt.v9i2.19290

Abstract

The information about the optimum and tolerable salinity to support in the success of coral trout grouper?s seed culture is still limited until now. The aim of this study was to understand the effect of different salinity on growth, survival, feed conversion (FCR) and the profile of blood (hematocrit and hemoglobin) of coral trout grouper seed. The seed with total length 6.0 ± 0.5 cm and body weight 3.2±0.3 g were used as tested animals. Five different salinity treatments, namely: 10±1 ppt (A), 16±1 ppt (B), 22±1 ppt (C), 28±1 ppt (D), and the sea water (34 ppt) as a control (E) were undertaken in this study, with three replications for each treatment. Salinity values were reach by adding freshwater into the tested seawater. Water changing system in this study was performed in recirculation process, with 25% of water replacement every 7 days. The result of this study showed that the survival rate values were 100% for all treatments, indicating that coral trout grouper seed can be cultured in the range of salinity between 10 to 34 ppt.  The highest total length and body weight were recorded from treatment C (22 ppt), as well as noted to gain the lowest FCR. Correspondingly, the seed in treatment C also founded to have in the greatest amount of hematocrit and hemoglobin. Optimum salinity for rearing of coral trout grouper seeds  with  total length 6 cm was 22 ppt.  Keywords: salinity, growth, survival rate, feed conversion, blood profile
PERTUMBUHAN DAN KANDUNGAN NUTRISI IKAN KERAPU SUNU PLECTROPOMUS LEOPARDUS PADA PEMELIHARAAN DI KARAMBA JARING APUNG, TAMBAK DAN BAK Setiawati, Ketut Maha; Kusumawati, Daniar; Asih, Yasmina Nirmala; Slamet, Bejo
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (412.104 KB) | DOI: 10.29244/jitkt.v12i1.23339

Abstract

Pendederan kerapu banyak dilakukan di bak-bak semen dan hatchery, tetapi usaha pendederan juga dapat dilakukan di laut dengan menggunakan karamba jaring apung (KJA) maupun di tambak.  Penelitian ini bertujuan untuk menganalisa pertumbuhan, dan kandungan nutrisi pada kerapu sunu yang dipelihara pada lingkungan berbeda yaitu di KJA, tambak dan bak hatchery.  Benih ikan kerapu sunu berukuran panjang total 6,56±0,52cm dan berat 4,48±1,04 g dengan kepadatan 50 ekor/jaring dipelihara dalam jaring berukuran 1mx1mx1m dengan ukuran mata jaring 4 mm yang ditempatkan dalam KJA di laut (A), tambak (B) dan bak hatchery (C) sebagai perlakuan percobaan. Penelitian menggunakan rancangan acak lengkap dengan tiga perlakuan dan diulang 3 kali. Penelitian dilakukan selama 90 hari pemeliharaan, dan benih ikan kerapu sunu diberi pakan buatan komersial dengan kandungan protein 48%. Frekuensi pemberian pakan 2 kali sehari pada pagi dan sore hari.  Hasil penelitian menunjukkan bahwa pemeliharaan pada lingkungan berbeda tidak berpengaruh terhadap sintasan dan pertumbuhan mutlak panjang total namun berpengaruh terhadap pertumbuhan mutlak bobot benih (P<0,01). Pertumbuhan mutlak benih terbaik pada pemeliharaan di bak hatchery yakni 23,12±2,91 g, KJA 8,43±2,13 g dan tambak 12,58±2,58 g.  Kandungan protein benih ikan kerapu sunu tidak dipengaruhi oleh lingkungan pemeliharaan, namun kandungan kalori benih pada pemeliharaan di bak hatchery dan tambak lebih tinggi dibandingkan dengan yang di KJA.  Benih ikan kerapu sunu yang didederkan dalam bak hatchery memiliki pertumbuhan bobot mutlak 1,84 kali lebih tinggi dibandingkan dengan yang di tambak atau 2,74 kali dari yang  di KJA.
PERKEMBANGAN MORFOLOGI DAN PERILAKU LARVA IKAN KERAPU HIBRIDA CANTIK PADA PRODUKSI MASSAL Ismi, Suko; Hutapea, Jhon H.; Kusumawati, Daniar; Asih, Yasmina Nirmala
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 2 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (993.01 KB) | DOI: 10.29244/jitkt.v10i2.21825

Abstract

Ikan kerapu cantik merupakan hibrida antara betina kerapu macan E. fuscoguttatus dan jantan kerapu batik E. microdon. Penelitian ini bertujuan untuk mengamati perkembangan dan tingkah laku larva ikan kerapu cantik agar bisa diambil langkah-langkah untuk perbaikan pada pemeliharaan larva sehingga dapat meningkatkan produksi. Penelitian dilakukan di Balai Besar Riset Budidaya Laut dan Penyuluhan Perikanan Gondol, Bali Tahun 2016. Larva dipelihara pada tiga buah  bak semen ukuran 10 m3  yang diisi telur dengan kepadatan 50.000 butir/bak, dipelihara hingga juvenil umur 40 hari (D40). Pemeliharaan larva diulang tiga kali dengan waktu yang berbeda, yaitu siklus 1: Januari-Maret; siklus 2 : Mei-Juli dan siklus 3 : September-Nopember. Pengamatan perkembangan dan tingkah laku larva dilakukan pada setiap siklus yang   dibagi menjadi 4 kelompok umur, yakni: 1) D1 sampai D10; 2). D11 sampai D20; 3). D21 sampai D30; dan 4). D31 sampai D40. Hasil menunjukkan larva ikan kerapu cantik mulai buka mulut dan makan pada D3, aktif berenang setelah tumbuh sirip punggung dan dada yaitu D4, sirip terus berkembang menjadi duri memanjang. D7 kondisi larva terlihat padat dilakukan penjarangan. Duri punggung dan dada mulai memendek, berubah menjadi sirip mulai D30, selanjutnya menjadi juvenil dilakukan grading untuk menekan kanibalisme, D40 dengan ukuran panjang total sekitar 3 cm larva ikan kerapu cantik seluruhnya menjadi juvenil. Perkembangan dan tingkah laku larva ikan kerapu cantik berpengaruh terhadap hasil produksinya.
THE EFFECTS OF FASTING DURATION AND DIFFERENT DENSITIES OF GROUPER SEED TRANSPORTED IN CLOSED SYSTEM Ismi, Suko; Kusumawati, Daniar; Asih, Yasmina Nirmala
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.528 KB) | DOI: 10.29244/jitkt.v8i2.15829

Abstract

Indonesia is an archipelagic country with many locations suit for aquaculture. Grouper hatcheries are still concentrated in a few locations. Therefore, appropriate technology to transport live grouper seed is needed to ensure the survival and health of the seed. This study aimed to determine the duration of fasting before transportation and the optimum density of grouper seed during transportation. Transportation was conducted in closed system, transported using a car, for 24 hours. Grouper seed used for the experiment was cantik hybrid grouper, a cross-breed between female tiger grouper (Epinephelus fuscoguttatus) and male marbled grouper (Epinephelus microdon) with an average of size 7.0±0.32 cm. There were 2 experiments in this study. Experiment 1 was different durations of fasting before transprotation: A. 12 hours, B. 24 hours, C. 36 hours and D. 48 hours with the density of 150 seeds/boxes. The result of the first experiment was used to determine the experimental design of experiment 2, which consisted of 4 different densities per boxes: A. 125 seeds, B. 150 seeds, C. 175 seeds and D. 200 seeds. Both of the experiments were conducted in triplicates. The seeds were packed in plastic bags with a thickness 0.08 mm, length 120 cm x width 53 cm, filled with 10 liters of sea water. The results showed that the duration of fasting from 36 to 48 hours had the highest survival rate. Polinomial relation y = -0.0439x2 + 3.7696x + 17.075 with the value of R² = 0.966 illustrated that the optimum duration of fasting was 44 hours. There was a significant difference in densities of seeds during transportation (P<0.05), where the higher density lead to the lower survival rate. The density of 125 seeds/boxes resulted in the highest survival rate, 99.0 ± 1.0%.
IMPROVEMENT OF SEED PRODUCTION AND QUALITY GROUPER BY HYBRIDIZATION PROGRAM Ismi, Suko; Asih, Yasmina Nirmala; Kusumawati, Daniar
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.202 KB) | DOI: 10.29244/jitkt.v5i2.7562

Abstract

Several types of grouper hybrid seeds can now be massively produced in a hatchery. Hybrid seeds can increase diversification of aquaculture species and potential to increase fish production.  Therefore, an  improvement in hybrid seed production both in high quantity and quality is very important. This research was conducted to produce massively cantik grouper hybrid seeds i.e.,a crossbreed between female tiger grouper (Epinephelus fuscoguttatus) and male marbled grouper (Epinephelus microdon). This research examined the cantik grouper seed production compared with the production of tiger grouper and marbled grouper fingerlings. The research results showed that cantik hybrid grouper seeds production had higher survival rate (24.59%) than tiger grouper (17.44%) and marbled grouper (4.63%). The total length of the seed at the age of 45 days for cantik grouper  was 3.59 ± 0.21 cm, tiger 3.24 ± 0.55 cm, and batik 2.61 ± 0.42 cm, respectively. Seed abnormality for cantik grouper was 4.13%, tiger grouper 30.21%, and marled tiger 0.57%, respectively. Based on genetic variation analyses, the cantik grouper had a closer genetic relationship with the marbled grouper compared with the tiger grouper. Keywords: Marbled grouper, hybridization program, seeds production, quality
GROW OUT CULTURE OF CORAL TROUT PLECTROPOMU LEOPARDUS IN FLOATING NET CAGES WITH DIFFERENT FEEDING FREQUENCIES Setiawati, Ketut Maha; Zafran, .; Kusumawati, Daniar
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.784 KB) | DOI: 10.29244/jitkt.v8i2.15827

Abstract

A study on the effect of feeding frequency on coral trout culture Plectropomus leopardus was conducted in floating net cages. The aim of this study was to determine an optimum feeding frequency for better growth rate and feed conversion ratio. The experiment was divided into two stages, i.e.; using fish with a range of body weight of 24 to 100 g (1st stage), and fish with a range of 100 to150 g (2nd stage). The first stage, consisted of four treatments of feeding frequencies, i.e. four times a day (4/1), three times a day (3/1), twice a day (2/1), and once a day (1/1). For the second stage experiment, the treatments applied were, three times a day (3/1), twice a day (2/1), once a day (1/1), and once in two days (1/2). Each treatment was in triplicate. At each feeding time, experimental fish were fed with dry pellets to apparent satiation. Both first and second experimental stages showed that the treatments of twice a day (2/1) feeding frequency attained significantly higher growth rates and lower feed conversion ratios than other treatments (P<0.05). Survival rate at the first stage (4/1), three times a day (3/1), twice a day (2/1), higher than (1/1) (P<0.05). But in the second stage the survival rate is not significant. The most optimum feeding frequencies for this species is 2/1 for the fish of 24 to 150 g BW.
APLIKASI ASTAXANTHIN DARI HAEMATOCOCCUS PADA BENIH KERAPU SUNU (PLECTROPOMUS LEOPARDUS) TERHADAP TOTAL KAROTENOID DAN PROFIL DARAH Kusumawati, Daniar; Mahardika, Ketut; Setiawati, Ketut Maha
Media Akuakultur Vol 14, No 2 (2019): (Desember, 2019)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.706 KB) | DOI: 10.15578/ma.14.2.2019.113-122

Abstract

Haematococcus merupakan alga yang kaya karotenoid dari jenis astaxanthin yang tidak hanya berpotensi sebagai sumber pigmen merah tetapi juga sebagai antioksidan. Aplikasi haematococcus telah dilakukan pada larva kerapu sunu dan menunjukkan adanya perbaikan peformansi warna merah yang cukup signifikan. Tujuan dari penelitian ini adalah mengetahui pengaruh haematococcus sebagai sumber astaxanthin terhadap pertumbuhan, total karotenoid, dan profil darah (hematokrit dan haemoglobin) pada juvenil ikan kerapu sunu. Benih kerapu sunu ukuran panjang rata-rata 14,07 ± 0,07 cm dan bobot rata-rata 45,92 ± 6,35 g dipelihara dalam jarring berukuran 0,5 m x 0,5 m x 1 m yang diletakkan dalam bak beton ukuran 3 m x 1,2 m x 1,2 m. Kepadatan ikan tiap jaring adalah lima ekor. Perlakuan yang diujicobakan adalah penambahan haematococcus ke dalam pakan buatan dengan dosis dan kompisisi sebagai berikut: A) 1% dari berat pakan, B) 1% dari berat pakan + 10% minyak ikan, dan C) 0% (kontrol). Hasil penelitian menunjukkan bahwa penambahan haematococcus ke dalam pakan tidak memberikan perbedaan nyata terhadap pertumbuhan mutlak panjang dan bobot, serta laju pertambahan panjang dan bobot (P value > 0,05). Penambahan haematococcus memberikan perbedaan nyata (P value < 0,05) terhadap konversi pakan di mana perlakuan A (1,99 ± 0,09); B (2,12 ± 0,14); dan C (2,28 ± 0,09). Penambahan haematococcus memberikan peningkatan terhadap akumulasi kandungan total karoten, hematocrit, dan haemoglobin darah.Haematococcus, an alga rich in carotenoids of the astaxanthin type, not only has the potential as a source of red pigment but also as an antioxidant. The purpose of this study was to determine the effects of hematococcus as astaxanthin source on the growth, total carotenoids, and blood profile (hematocrit and hemoglobin) of coral trout grouper juvenile. Coral trout grouper seed with an average length of 14.07± 0.07 cm and an average weight of 45.92 ± 6.35 g were maintained in a net cage measuring 0.5 m x 0.5 m x 1 m placed in a 3 m x 1.2 m x 1.2 m concrete tank. Fish density per net was five fish. The treatment tested was the addition of haematococcus into the artificial feed with the following dosages and compositions: A) 1% of the weight feed, B) 1% of the weight feed + 10% fish oil, C) 0% (control). The results showed that the addition of haematococcus to the feed did not give a significant difference to the absolute growth of length and weight and also specific growth and length rate (P-value > 0.05). The addition of haematococcus gave a significant difference (P-value < 0.05) to feed conversion ratio between treatment A, B and C with the values of 1.99 ± 0.09, 2.12 ± 0.14, and 2.28 ± 0.09, respectively. The addition of haematococcus also increased the levels of total carotene content, hematocrit, and hemoglobin. This study suggests that the application of haematococcus could significantly improve the red color performance of trout grouper larvae.
PERTUMBUHAN IKAN BANDENG (Chanos chanos) ANTARA BENIH HSRT DAN GENERASI KEDUA (G-2) TERSELEKSI [Growth Performance of Milkfish, (Chanos chanos) Between Small Scale Hatcheries and of Selected Second- Generation (G-2) Sources] Kusumawati, Daniar; Jamaris, Zafran; Aslianti, Titiek
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4230.251 KB) | DOI: 10.14203/beritabiologi.v17i1.2937

Abstract

Currently, there is a national concern regarding to the decrease of milkfish production from ponds in North Java due to a low quality of milkfish seed produced by small scale hatcheries in Bali, which is the main producer of milkfish seed. The quality of seed is strongly related to the quality of eggs (fertility), while quality of eggs produced depends on the management of brood stocks carried out by the hatcheries. Growth rate and growth hormone profiles are some of the biological aspects that could be used as a basis/benchmark to evaluate quality level of milkfish seed reared in ponds. The aim of this experiment was to evaluate growth rate and growth hormone profile performance between selected G-2 seed and small scale hatcheries seed as control population the growth rate. Research on grow-out of milkfish seed was conducted at the IMRAD ponds facility in Pejarakan, using milkfish seed produced by small scale hatcheries as well as selected second-generation (G-2) seed, each with the density of 5000 seed/pond (1 pond=0.5 ha). The seeds were fed with dry pellet and reared for 5 – 6 months. The results showed that the seed produced with standard operational procedure (SOP) by small scale hatcheries were having longer (F hit. = 13.68 > F tabel 1%) and heavier body washt (F hit. = 18.98 > F tabel 1%) better than selected G-2 seed and small scale hatcheries seed without SOP with high growth hormone concentration (F hit. = 4.95 > F tabel 5%).
LAJU PENGOSONGAN LAMBUNG PADA LARVA DAN BENIH IKAN KLON ( Amphiprion ocellaris ) Setiawati, Ketut Maha; Imanto, Philip Teguh; Kusumawati, Daniar
Jurnal Riset Akuakultur Vol 7, No 1 (2012): (April 2012)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.435 KB) | DOI: 10.15578/jra.7.1.2012.33-39

Abstract

Penelitian ini bertujuan untuk mengetahui waktu yang dibutuhkan untuk pengosongan lambung baik pada larva maupun benih ikan klon sebagai dasar manajemen pemberian pakan pada larva maupun benih ikan klon. Penelitian ini dilakukan pada larva umur 10 hari dengan panjang total 7,07±0,50 mm, dan benih umur 2 bulan dengan panjang total berkisar antara 2,64±0,23 cm. Masing-masing hewan uji tidak diberi pakan dari pukul 15.00 WITA (puasa selama 18 jam), kemudian keesokan harinya pukul 09.00 hewan uji diberi pakan buatan sampai kenyang untuk benih, sedangkan untuk larva diberi pakan Artemia selama 1 jam, setelah itu, air pada bak larva dialirkan agar pakan yang tersisa terbuang. Pengambilan sampel dilakukan setiap 1 jam pada larva sedangkan untuk benih dilakukan setiap 2 jam sekali. Hasil penelitian menunjukkan bahwa pada jam ke-4 isi lambung pada larva umur 10 hari sudah mulai kosong, sedangkan pada benih ikan klon isi lambung akan kosong setelah 11 jam 37 menit sejak pemberian pakan terakhir.
PERAN GEN AIM1 DAN INTENSITAS CAHAYA TERHADAP KARAKTER POLA PIGMEN IKAN BADUT HITAM (Amphiprion percula) Kusumawati, Daniar; Permana, S.; Setiawati, Ketut Maha; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 7, No 2 (2012): (Agustus 2012)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.693 KB) | DOI: 10.15578/jra.7.2.2012.205-219

Abstract

Pola pigmen merupakan faktor utama yang menentukan tingginya kualitas ikan hias. Pada benih-benih F1 populasi budidaya ikan badut hitam (Amphiprion percula) diketahui mengalami degeneratif pola pigmen yang menyimpang dibandingkan dengan populasi di alam. Pola pigmen merupakan salah satu karakter fenotip yang diturunkan di mana ekspresinya bergantung pada interaksi genetik dengan faktor eksternal yaitu lingkungan. Tujuan dari penelitian ini adalah untuk mengkaji peran gen yaitu Aim1 yang mengontrol sintesis melanin dan kondisi lingkungan dalam menginduksi pola pigmen hitam ikan badut hitam (Amphiprion percula). Analisis gen Aim1 dilakukan dengan menggunakan program speedy PCR dilanjutkan dengan SSCP (single strand confirmation polymorphism) untuk mengidentifikasi variasi genetik dari gen Aim1. Analisis SDS Page dilakukan untuk mengetahui peranan lingkungan terhadap profil protein yang disintesis. Berdasarkan hasil penelitian diketahui bahwa primer Aim1 memiliki sisi pengenalan pada whole genom ikan badut hitam (Amphiprion percula) pada target sequence 45 bp. Berdasarkan analisis SSCP profil fragmentasi amplicon primer Aim1 pada masing-masing tingkatan pada populasi budidaya homolog dengan populasi alam, sehingga gen Aim1 bukan merupakan gen yang mengontrol fenomena degeneratif pola pigmen ikan badut hitam (Amphiprion percula). Peran lingkungan yaitu cahaya memberikan pengaruh positif dalam menginduksi pola pigmen melalui stimulus pada sistem neuron dan migrasi melanophore.