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PROFIL LEUKOSIT, DIFERENSIAL LEUKOSIT, DAN INDEKS STRES LUWAK JAWA (PARADOXURUS HERMAPHRODITUS) (LEUCOCYTE COUNT, LEUCOCYTE DIFFERENTIATION, AND STRESS INDEX OF COMMON PALM CIVETS (PARADOXURUS HERMAPHRODITUS) Satyaningtijas, Aryani Sismin; Kusumorini, Nastiti; Fachrudin, Moh. Mursyid; ., Purnomo
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Common palm civets (Paradoxurushermaphroditus) have a potential to be commercial commodityanimal which produce best coffee, parfume, but also as reservoir potential infection desease such as SevereAcute Respiratory Syndrome (SARS). Therefore, their health status is very important. This study wasaimed to discribe the leukocyte profile, and stress index of 4 males Java common pal civets and 4 females.Blood sampling was taken from vena femoralis and done at weeks 1st, 5th, 6th, and 7th. Leukocyte profilewere examined using haemocytometerand blood smear. The average leukocyte count of males and femalescommon palm civets per mm3 were (3.33±0.86)x103 and (2.83±0.70)x103, neutrophil were (1.01±0.47)x103and (0.68±0.30)x103, eosinophil were (0.16±0.18)x103 and (0.04±0.05)x103, lymphocyte were (2.06±0.42)x103and (2.05±0.59)x103, monocyte were (0.09±0.06)x103 and (0.06±0.06)x103. Stress index were (0.49±0.18)for males and (0.37±0.22) for females. Generally, leukocyte count of males Java common palm civet werehigher than those of females.
PEMBERIAN VAKSIN ICHTHYOPHTHIRIUS MULTIFILIIS UNTUK MENCEGAH ICHTHYOPHTHIRIASIS PADA IKAN MAS (APPLICATION OF ICHTHYOPHTHIRIUS MULTIFILIIS VACCINE FOR PREVENTION OF ICHTHYOPHTHIRIASIS IN COMMON CARP) Syawal, Henni; Kusumorini, Nastiti; Manalu, Wasmen; Affandi, Ridwan
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was conductedto assess the physiological responses of commoncarp due to administrationof ich vaccines and kept at different water temperatures. Completely Randomized Factorial Designwasusedin this study. The number and the size of the experimental fish was720 and 5-7 cm, respectively. Fish wasvaccinated by immersing the fish in water that contained 3 mLL-1 of ich vaccines for 15 minutes. Then, fishwas kept for 21 days at various temperatures: at 18ºC which was room temperature without heater andfish had not been vaccinated and at 20, 24, and 28ºC where fish was given the vaccines and the aquariumis facilitated with electric heater. Atday15post immunization, challenge test with 5.000 cells of life therontfish-1was performed.Immobilization test, differential leukocyte, prevalence rates, and survival rates weremeasured. The measurements of these parameters were performed four times:before the treatment (day0), day7, day14, and day21, respectively. The results showed that the vaccine could reduce the stress inthefish that were kept at 20, 24, and 28ºC, and the prevalence of ichthyophthiriasis. In addition the vaccineincrease the fish survival rates after challenge test. The highest survival rate (100%) was found in fish thatwerekept in 28ºC.
FRAKSI HEKSAN DAUN KATUK SEBAGAI OBAT UNTUK MEMPERBAIKI PRODUKSI SUSU, PENAMPILAN INDUK, DAN ANAK TIKUS (HEXANE FRACTION OF SAUROPUS ANDROGYNUS LEAVES AS A MEDICINE FOR IMPROVING MILK YIELD, THE PERFORMANCE OF FEMALE, AND RAT PUPS) Suprayogi, Agik; Kusumorini, Nastiti; Arita, Syaprianti Evi Dame
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study of Sauropus androgynus leaves fractions as a medicine for improving milk yield, the performanceof dam, and rat pups was conducted on five groups of lactating pregnant rats. Each group consists of 16rats, namely hexane fraction (FH), ethyl acetate (Fets), water (FH2O), ethanol crude extract (EEto); andcontrol group. The administration of fractions and crude extract were executed orally through feed aftereight days of mice gestation period. The average consumption dose was 57.5 mg/day, 40 mg/day, 209 mg/day, 297.5 mg/day, and 0 mg/day, respectively. The provision of treatment for 12 days gestation periodshowed no significant effect on the average daily feed intake. The FH administration did not demonstratedifferences of growth and body weight gain of dams in comparison to the control group. Whereas theadministration of FETs, FH2O, and EE to showed the growth inhibition significantly. The FH administrationshowed gradually increase of rat pups growth and body weight gain significantly in pups 7-10 days oldcompared to the control group. However, there were signs of growth inhibition on the other groups. The FHgroup revealed a positive response on the total milk yield for 10 days of lactation, compared to the othergroups. Hexane fraction has the potency as a remedy for improving milk yield without influencing the damand rat pups performance. This study also indicates side effect on growth inhibition, especially on theadministration of ethyl acetate and water fraction, and ethanol crude extract.
PENGENDALIAN KADAR GLUKOSA DARAH OLEH TEH HIJAU DAN ATAU TEH DAUN MURBEI PADA TIKUS DIABETES Efendi, Rusman; Damayanthi, Evy; Kustiyah, Lilik; Kusumorini, Nastiti
Jurnal Gizi dan Pangan Vol. 5 No. 2 (2010)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.237 KB) | DOI: 10.25182/jgp.2010.5.2.87-94

Abstract

Diabetes mellitus is degeneratif disease with high prevalence that happens in many countries. Several studies had been done to control diabetes by using green tea, mullberry leaf  tea, and their mixture. The aim of this research was to analyze the influence of the administration green tea, mullbery leaf tea, and their mixtures to blood glucose level of diabetic rats both during 120 minutes after administration. This research had four phases, first to determine the best mullberry leaf tea, second to fourth phases respectively, determine turnover of blood glucose level on normal rats; attempt during 120 minutes on diabetic rats.  The result of research during 120 minutes have showed that blood glucose level on diabetic rats which were administered by green tea, mullberry leaf tea and their mixture is significantly difference with diabetic rats which were administered by water. Blood glucose level at baseline increased at 30th minutes and showed the difference significantly and then until 60th and 120th minutes and relatively stable. During 120 minutes after feed consumption, inhibition of blood glucose level occured increasingly on diabetic rats which were administered by green tea, mullberry leaf tea, and their mixture compared to diabetic rats which were administered by water.
KADAR ESTROGEN DAN PROFIL OVIDUK PUYUH (COTURNIX COTURNIX JAPONICA) SETELAH PEMBERIAN CAHAYA MONOKROMATIK Kasiyati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.577 KB) | DOI: 10.14710/baf.v17i2.3829

Abstract

Light plays an important role in the avian life. The present study was designed to evaluate the effects of monochromatic light on serum estrogen concentrations and profile the oviduct, that were the length and weight of the oviduct in the quail. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light,  controls  with 15 and 25 W, red, green, and blue lights with intensities of  15 and 25 lux. Control  treatment used incandescent bulb.  The red,  green,  and  blue lights  were  provided  by   light emitting   diodes  (LED). All lights   treatment   were   given   for   14 h  daily,   started from 17.00 to 07.00. Parameters   measured  were  serum estrogen  concentrations, and  weights  and  lengths  of   the  oviduct  on  weeks 5,  7,  and  9.  Quails   exposed  to  monochromatic   light had  higher  serum  estrogen  concentrations (P<0,05). Quails  exposed to monochromatic light had  better  weights  and  lenghts of  the  oviduct  development. Blue light could be used to increase serum estrogen concentrations and stimulate shell gland development.
PENERAPAN CAHAYA MONOKROMATIK UNTUK PERBAIKAN KUANTITAS TELUR PUYUH (COTURNIX COTURNIX JAPONICA.L) Kasiati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 19, No 1 (2011) : Volume XIX, Nomor 1, Maret 2011
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.969 KB) | DOI: 10.14710/baf.v19i1.2580

Abstract

The quail?s egg have value of nutrient which similar to the chicken?s egg and the duck?s egg. Various of the programme of the light to improve repair of the quantity and the quality of the quail egg. The objectives of this research were to applied monochromatic light to repair the quality and quantity of the quail egg. Two hundred and seventy female quails (DOQ) were divided into nine treatments of light, with ten replications and three quails in each replication, the treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were amount of the egg weekly, henday egg production, and quality of the egg consist of the weights of the egg, protein and fat of the egg. The result showed that quails exposed to blue light had higher of the amount of the egg, henday production, and the weights of the egg, while the quality of the egg, such as protein and fat in the egg were not change (P>0,05). Blue light could be used to improve repairing quantities of the quail egg.
KAJIAN FISIOLOGIS STATUS KALSIUM PUYUH (COTURNIX COTURNIX JAPONICA) SETELAH PEMBERIAN CAHAYA MONOKROMATIK Kasiyati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.765 KB) | DOI: 10.14710/baf.v18i1.2588

Abstract

Deposited calsium makes of the eggshell is influnce of the light. The objective of the study was evaluate exposed of monochromatic light to obtain basic information of the physiology aspect of calcium satus in the quail which direct relation with quality of the eggshell. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were serum calcium concentrations, calcium contents in bone and the eggshell, weights and thichness of the eggshell. The data obtained were analyzed by analysis of variance (ANOVA), and the differences between treatments groups were subjected for Duncan?s Multiple Range Test (DMRT). All of the data analyzed were done GLM (general linear model) procedure in the SAS program. Quails exposed to monochromatic light had higher serum calcium concentrations and calcium contents in bone (P<0,05). Blue light could be used to increase quality of the eggshell.
PENURUNAN KADAR PROGESTERON SERUM DAN KOMPONEN MATRIKS EKSTRASELULER DAN SELULER KULIT SEBAGAI INDIKATOR PENUAAN PADA TIKUS s, Safrida; Kusumorini, Nastiti; Manalu, Wasmen; Maheshwari, Hera
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.557

Abstract

Penelitian ini bertujuan mengetahui kadar kolagen kulit (matriks ekstraseluler), jumlah sel (kadar DNA), aktivitas sintetik (kadar RNA) pada jaringan kulit, serta kadar hormon progesteron pada berbagai tingkatan umur tikus, dan memperoleh umur tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri atas sembilan kelompok percobaan masing-masing terdiri atas tiga ekor tikus, yaitu umur 12 bulan (K1), 18 bulan (K2), 24 bulan (K3), 30 bulan (K4), 36 bulan (K5), umur 12 bulan dalam kondisi 1 bulan pascaovariektomi (K6), 12 bulan dalam kondisi 3 bulan pascaovariektomi (K7 ), 18 bulan dalam kondisi 1 bulan pascaovariektomi (K8), dan umur 24 bulan dalam kondisi 1 bulan pascaovariektomi (K9). Data yang diperoleh dianalisis dengan menggunakan analisis varian dan dilanjutkan dengan uji Duncan. Komponen matriks ekstraseluler dan matriks seluler menurun seiring dengan bertambahnya usia. Umur tikus normal yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 24-36 bulan. Tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 12 bulan pascaovariektomi 3 bulan.
PERUBAHAN KUALITAS SPERMATOZOA DAN JUMLAH SEL-SEL SPERMATOGENIK TIKUS YANG TERPAPAR ASAP ROKOK Jems Akiles Unitly, Adrien; Kusumorini, Nastiti; Agungpriyono, Srihadi; Sismin Satyaningtijas, Aryani; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2629

Abstract

Penelitian ini bertujuan mengetahui efek asap rokok terhadap perubahan kualitas spermatozoa dan jumlah sel-sel spermatogenik. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan asap rokok 10 batang/ekor/hari selama 2,5 jam dalam smoking chamber terhadap 24 ekor tikus jantan yang dibagi menjadi 4 kelompok, masing-masing 6 ekor dalam tiap kelompok. Kelompok P1 adalah kelompok yang tidak diberi perlakuan (kontrol), P2 adalah kelompok yang dipapar asap rokok selama 20 hari, P3 adalah kelompok yang dipapar asap rokok selama 40 hari, P4 adalah kelompok yang dipapar asap rokok selama 60 hari. Parameter yang diamati adalah kualitas spermatozoa dan jumlah sel-sel spermatogenik. Hasil penelitian menunjukkan bahwa pemaparan asap rokok dapat menurunkan konsentrasi spermatozoa dan viabilitas spermatozoa serta meningkatkan abnormalitas spermatozoa. Disimpulkan bahwa pemaparan asap rokok 10 batang/ekor/hari menyebabkan penurunan kualitas spermatozoa dan jumlah sel-sel spermatogenik yang tidak kembali ke kondisi normal setelah proses penyembuhan.
THE PEARL SAC FORMATION IN MALE AND FEMALE PINCTADA MAXIMA HOST OYSTERS IMPLANTED WITH ALLOGRAFT SAIBO Eddy, La; Affandi, Ridwan; Kusumorini, Nastiti; Sani, Yulvian; Manalu, Wasmen
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2869.656 KB) | DOI: 10.4308/hjb.22.3.122

Abstract

An experiment was conducted to study the effect of male and female host oysters on the pearl sac formation in Pinctada maxima oyster. One hundred sixty oysters were used in a completely randomized design with 2 x 4 factorial arrangement and 20 replications. The 1st factor was that sex of host oyster consisted of two levels that is males and females. The 2nd factor was week after nucleus implantation with four levels that is 1, 2, 3, and 4 weeks. The parameters observed were the percentage of successful oysters to form the pearl sac, the speed of pearl sac formation, the percentage of nucleus coverage by the pearl sac, histology of the pearl sac growth and development, and haemolymph glucose, calcium and phosphorus concentrations. Our results showed that the percentages of host oysters that succeeded in forming a pearl sac were 80% and 75% in female and male host oysters, respectively. There was no statistical difference in nucleus rejection and mortality in male and female host oysters but the results indicated that male host oysters showed a numerically higher nucleus rejection. The speed of pearl sac growth and the percentage of nucleus coverage by the pearl sac in female host oysters were better than those in male host oysters. Haemolymph calcium, phosphorus and glucose concentrations, oxygen consumption, and histological development of the pearl sac were not different between male and female host oysters. Pearl sac formation in the female host oysters was better than that in male host oysters.