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TRIMER RESVERATROL DARI KULIT BATANG SHOREA PARVIFOLIA DYER. Rosyidah, Kholifatu; Juliawati, Lia D.; Syah, Yana M.; Hakim, Euis H.; Achmad, Sjamsul A.; Makmur, Lukman; Din, Laily Bin; Latip, Jalifah; Said, Ikram M.
Jurnal Sains dan Terapan Kimia Vol 1, No 1 (2007)
Publisher : Jurnal Sains dan Terapan Kimia

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Abstract

Shorea atau yang dikenal sebagai meranti merupakan genus utama famili Dipterocarpaceae. Masyarakat banyak menggunakan tumbuhan ini sebagai bahan bangunan. Senyawa oligomer resveratrol banyak ditemukan dalam tumbuhan ini berupa dimer, trimer, dan tetramer resveratrol. Beberapa senyawa oligomer resveratrol yang berhasil diisolasi menunjukkan aktivitas biologi yang penting seperti anti-HIV, sitotoksik, anti-jamur, anti-inflamasi, dan anti-bakteri. Pada penelitian ini telah berhasil diisolasi senyawa trimer resveratrol dari ekstrak aseton kulit batang Shorea parvifolia Dyer. Struktur senyawa tersebut ditetapkan berdasarkan berdasarkan data spektroskopi UV, IR, and 1H-NMR.Kata kunci: trimer resveratrol, Shorea parvifolia Dyer., Dipterocarpaceae.
SENYAWA DIMER DAN TRIMER RESVERATROL DARI KULIT BATANG DIPTEROCARPUS INTRICATUS (DIPTEROCARPACEAE) DAN AKTIVITAS SITOTOKSIKNYA TERHADAP SEL MURIN LEUKIMIA P-388 Muhtadi, Muhtadi; Hakim, Euis H.; Syah, Yana M.; Juliawaty, Lia D.; Achmad, Sjamsul A.; Latip, Jalifah
Alchemy Jurnal Penelitian Kimia Vol 7, No 1 (2008)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Dua senyawa oligomer resveratrol, (-)-?-viniferin (2) dan (-)-?-viniferin (3), bersama-sama satu senyawa turunan asam fenolat, bergenin (1) telah berhasil diisolasi untuk pertama kalinya dari ekstrak aseton kulit batang Dipterocarpus intricatus. Struktur molekul senyawa-senyawa tersebut ditetapkan berdasarkan data spektroskopi UV, IR, 1H-NMR dan 13C-NMR serta perbandingan dengan data sejenis yang telah dilaporkan. Senyawa (-)-?-viniferin (2) dan (-)-?-viniferin (3)menunjukkan aktivitas sitotoksik terhadap sel murin leukemia P-388 dengan nilai IC50 masing-masing sebesar 7,8 dan 17,5 ?g/mL.
AMPELOPSIN A DAN α-VINIFERINDARI KULIT BATANG SHOREA GIBBOSA BRANDIS (DIPTEROCARPACEAE) Saroyobudiyono, Haryanto; Hakim, Euis H.; Syah, Yana M.; Achmad, Sjamsul A.; Juliawaty, Lia D.; Latip, Jalifah
Alchemy Jurnal Penelitian Kimia Vol 4, No 2 (2005)
Publisher : Alchemy Jurnal Penelitian Kimia

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Abstract

Dua senyawa oligomer resveratrol yaitu (-)-ampelopsin A dan (-)-?-viniferin, telah berhasil diisolasi untuk pertama kalinya dari ekstrak aseton kulit batang Shorea gibbosa. Struktur senyawa ini telah ditetapkan berdasarkan data spektroskopi UV,IR, 1H NMR, 13C NMR serta melalui perbandingan terhadap data senyawa sama yang telah dilaporkan. Pengujian sifat sitotoksikbterhadap dua senyawa oligomer resveratrol tersebut menunjukkan hasil IC50 berturut-turur adalah 17,0 µg.mL-1 ,dan 18,2 µg.mL-1.
Morphological studies of apoptotic HeLa cells death induced by eurycomanone ., Nurkhasanah; Pihie, Azimahtol Hawariah Lope; Latip, Jalifah
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.617 KB) | DOI: 10.14499/indonesianjpharm0iss0pp190-197

Abstract

Eurycomanone is a cytotoxic ingredient found in Eurycoma longifolia Jack. Previous studies have noted its activity against many epithelial cell lines. In this study, eurycomanone had obvious cytotoxic effect on HeLa cells by methylene blue staining assays. After HeLa cells were treated with eurycomanone, typical morphological changes, including cytoplasm shrinkage and decrease of cell volume were observed by light microscope. Chromatin condensation and nuclear fragmentation could be observed by fluorescence microscope after staining with Hoechst 33258 nuclear staining. DNA fragmentation and apoptotic body formation that is characteristic of apoptosis could be determined after treated cells were assayed with TUNEL (terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling). The externalization of phosphatidyl serine (PS) could be determined by flow cytometry analysis using Annexin-V/PI double staining. The result suggested that eurycomanone exerted antiproliferative activity on HeLa cells by inducing apoptosis.Key words : eurycomanone, cytotoxic, apoptosis, HeLa
Three resveratrol’s oligomers from the stem bark of H. gregaria (dipterocarpaceae) and their toxicity and citotoxicity ., Sahidin; Hakim, Euis H.; Syah, Yana M.; Juliawaty, Lia D.; Achmad, Sjamsul A.; Latip, Jalifah
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 3, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.948 KB) | DOI: 10.14499/indonesianjpharm0iss0pp109-115

Abstract

Three resveratrol oligomers have been isolated from the stem bark of H. gregaria including a resveratrol trimer i.e. α-viniferin (1), and two resveratrol tetramers i.e. vaticanol B (2) and hopeaphenol (3). These structures were determined by spectroscopic evidence including UV, IR, 1H , 13C NMR and compared with that of the reported data in the literatures. Activities of the compounds were determined toward brine shrimp Artemia salina (toxicity) and murine leukemia P-388 cells (cytotoxicity). The compound 1 is the most active against A. salina (LC50 = 49,0 ± 5,8 μg/mL), and the compound 3 has very active level toward murine leukemia P-388 cells (LC50 = 5,7 ± 0,3 μM).Key words : Resveratrol oligomers, H. gregaria, toxicity, and cytotoxicity.
RESVERATROL DIMERS FROM STEM BARK OF Hopea gregaria AND THEIR CYTOTOXIC PROPERTIES Sahidin, Sahidin; Hakim, Euis Holisotan; Syah, Yana Maolana; Juliawaty, Lia Dewi; Achmad, Sjamsul Arifin; Bin Din, Laily; Latip, Jalifah
Indonesian Journal of Chemistry Vol 8, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.862 KB) | DOI: 10.22146/ijc.21629

Abstract

Six resveratrol dimers have been isolated from the stem bark of H. gregaria, ampelopsin A (1), balanocarpol (2), ε-viniferin (3), hopeafuran (4), heimiol A (5), and parviflorol (6). The structures of these compounds were determined based on spectroscopic evidence such as UV, IR, 1-D, 2-D NMR and comparison with the reported data. These compound inventions are strengthen conclusion that Hopea tends to produce resveratrol dimers. Biological activity of those compounds against murine leukemia P-388 cells showed that ε-viniferin (3) is the most active compound with IC50 value 5.1 ± 0.3 μg/mL.   Keywords: H. gregaria, resveratrol dimers, murine leukemia P-388 cells
Medicinal Plants: A Prospect in Developing Male Fertility Enhancing Agent Luthfi, Muhammad Ja’far; Noor, Mahanem Mat; Latip, Jalifah
Biology, Medicine, & Natural Product Chemistry Vol 4, No 2 (2015)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.601 KB) | DOI: 10.14421/biomedich.2015.42.41-47

Abstract

Medicinal plants have been a revolutionary breakthrough in the treatment of male sexual dysfunction. Traditional medicine based on a holistic philosophy is quite different with the practice of “western” medicine. Phytochemical substances focus their mechanisms of healing to the root of cause, i.e. the inability of controlling the proper function of the whole body system. Hence, medicinal plants manage sexual dysfunction and male fertility in the frame of sexual dysfunction as a whole entity. Some previous researches prove that the use of medicinal plants have a good impact in the treatment of a variety of male sexual problems. This paper will discuss several important aspects of aphrodisiac plants and preliminary study regarding them in Indonesia. Difficulties inherent to activity guided isolation and the specific requirements of bioassays are also discussed.
KAJIAN FITOKIMIA Hopea mengarawan DAN IMPLIKASINYA PADA KEMOTAKSONOMI HOPEA Sahidin, Sahidin; Hakini, EuisH; Syah, Yana M; Juliawaty, Lia D; Achmad, Sjamsul A.; Din, Laily Bin; Latip, Jalifah
BERITA BIOLOGI Vol 8, No 2 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1360.734 KB) | DOI: 10.14203/beritabiologi.v8i2.2036

Abstract

Nine resveratrol oligomers have been isolated from the stem bark of Hopea mengarawan i.e. diptoindonesin D (1), balanocarpol (2), ampelopsin A (3), hopeaphuran (4), heimiol A (5), parviflorol (6), a-viniferin (7), isohopeaphenol (8), and vaticanol B (9).The structures of these compounds were determined based on spectroscopic evidence such as UV, IR, 1-D, 2-D NMR and comparison with the reported data. Those compounds implied that Hopea tends to produce resveratrol dimers. Moreover, in subgenus level, besides ampelopsin A (3) and isohopeaphenol (9), resveratrol oligomers in H. mengarawan the same as in H.dryobalanoides. It indicated that H. mengarawan and H. dryobalanoid.es came from the same subgenus.
UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH Sami, Fitriyanti Jumaetri; Soekamto, Nunuk Hariani; Firdaus, Firdaus; Latip, Jalifah
Jurnal Kimia Riset Vol 4, No 1 (2019): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (30.811 KB) | DOI: 10.20473/jkr.v4i1.10903

Abstract

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of  T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL. 
Tocotrienol Rich Fraction from Palm Oil Reduces Plasma and Erythrocyte Membrane Lipid Alteration in Diabetic Rats: Tocotrienols Reduce Dyslipidemia in Plasma and Erythrocytes Alwahaibi, Nasar; Budin, Siti Balkis; Hamid, Zariyantey Abdul; Mohamed, Jamaludin; Latip, Jalifah; Ismail, Norzamzurina Binti; Ayatillah, Alefee Bin; Matough, Fatmah Ali
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.691

Abstract

BACKGROUND: Tocotrienol-rich fraction (TRF) from palm oil has been shown to exhibit potent antioxidative properties in various pathological conditions. This study aimed to determine the antioxidative properties of TRF on the plasma and erythrocyte membrane lipids composition in streptozotocin-induced diabetic rats.METHODS: Male Sprague-Dawley rats (n=40) were divided randomly into four groups of 10, which were a normal group, a normal group with TRF, a diabetic group, and a diabetic group with TRF. Following four weeks of treatment, lipids compositions in the plasma and erythrocyte membrane were investigated.RESULTS: TRF significantly reduced the plasma total cholesterol, to that in the diabetic group. In addition, supplementation of TRF was able to reduce the very low density lipoprotein cholesterol (VLDL-C), triglycerides levels in treated diabetic rat although no significant different was observed when compared with untreated diabetic rats alone. Furthermore, TRF supplementation showed increased the levels of phosphatidylinositol, phosphatidylserine, and total phospholipid levels in diabetic rats. However, the linoleic acid (18:2) levels were reduced in diabetic rats compared to the control group, and the eicosadienoic acid (20:2) levels were significantly increased by TRF supplementation in diabetic rats.CONCLUSION: Supplementation with 200 mg/kg TRF improves dyslipidemia and that TRF, therefore, might have protective effects against oxidative damage of phospholipids. This study also showed the antioxidative properties of TRF on the erythrocyte membrane lipids composition, in particular, its protective effects against peroxidation of unsaturated fatty acids.KEYWORDS: antioxidative property, cholesterol, fatty acids, phospholipids, tocotrienol-rich fraction