Articles

REVIEW: HEPATOPROTECTOR COMPOUNDS IN PLANT EXTRACTS ZAIN, DICHY NURYADIN; Amalia, Riezki; Levita, Jutti
Indonesian Journal of Applied Sciences Vol 8, No 1 (2018)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.095 KB) | DOI: 10.24198/ijas.v8i1.16967

Abstract

Many plants have been reported to possess hepatoprotective activity. The mechanism has not yet been revealed, but it was predicted due to the antioxidants that could prevent the formation of free radicals produced by hepatotoxins. Of the 30 plants studied, Crocus sativus gave the largest hepatoprotector activity at a dose of 20 mg / kg, followed by Uncaria gambier Roxb. with a dose of 30 mg / kg and Melastoma malabathricum Leaf at a dose of 50 mg / kg. Keywords: Liver, CYP450, antioxidant, free radicals
RED GINGER (ZINGIBER OFFICINALE VAR. RUBRUM): ITS CHEMICAL CONSTITUENTS, PHARMACOLOGICAL ACTIVITIES AND SAFETY Supu, Rini Daud; Diantini, Ajeng; Levita, Jutti
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 8, No 1 (2018): Fitofarmaka Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.437 KB) | DOI: 10.33751/jf.v8i1.1168

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Ginger (Zingiber officinale) which belongs to the Zingiberaceae family, was first cultivated in Asia (Indonesia and Malaysia). This plant is one of the most commonly used herbal supplements taken by many patients to treat various conditions. Z.officinale has three varieties based on its size, colors of rhizome and chemical constituents i.e. .Z. officinale var. officinale (big white ginger or giant ginger, badak or gajah), Z. officinale var. amarum (small white ginger, emprit), and Z. officinale var. rubrum (small red ginger, merah or beureum). These three varieties may partly be deferred from their essential oil contents and are used for different purposes. The essential oils contained in Z. officinale var. rubrum are higher than the other types of ginger, which makes stronger in its pungency smell and taste. There are many studies that confirm beneficial effects of red ginger against the symptoms of diseases, i.e. anti-inflammation, antioxidant, antiemetic, antibacterial and antidiabetics. Z.officinale var. rubrum is considered to be a safe herbal medicine with only few and insignificant adverse/side effects. Although the medicinal properties of red ginger have been known, further trials in humans are required to determine the efficacy of red ginger (or one or more of its constituents) and to establish what, if any, adverse effects are observed.
KADAR KALKON TOTAL DI DALAM EKSTRAK ETANOL BATANG ASHITABA (ANGELICA KEISKEI KOIDZUMI) Pebiansyah, Anisa; Amalia, Riezki; Aulifa, Diah Lia; Levita, Jutti
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 9, No 2 (2019): Fitofarmaka Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.776 KB) | DOI: 10.33751/jf.v9i2.1579

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ABSTRAK Ashitaba (Angelica keiskei) merupakan salah satu tanaman obat yang terbukti memiliki aktivitas antioksidan setara dengan vitamin E. Aktivitas antioksidan umumnya ditunjukkan oleh metabolit sekunder tanaman, terutama flavonoid. Di dalam batang ashitaba terkandung senyawa kalkon yaitu xantoangelol (XAG) dan 4-hidroksiderisin (4-HD), yang tergolong ke dalam flavonoid dengan cincin C terbuka. Penelitian ini bertujuan  untuk  menetapkan  kadar  kalkon  total  (dihitung  sebagai  XAG)  di  dalam ekstrak etanol batang ashitaba. Tanaman ashitaba diperoleh dari Gunung Rinjani, Lombok, dan dideterminasi di Sekolah Ilmu dan Teknologi Hayati Institut Teknologi Bandung.  Penetapan  kadar  kalkon  total  dilakukan  menggunakan  metode spektrofotometri standar adisi. Hasil penapisan fitokimia menunjukkan bahwa ekstrak etanol batang ashitaba mengandung senyawa flavonoid, alkaloid, polifenol dan tanin, sedangkan kalkon total yang terkandung  di dalam ekstrak etanol kering batang ashitaba dihitung sebagai XAG adalah 0,836 % b/b.Kata kunci : Ashitaba, antioksidan, xantoangelol, 4-hidroksiderisin, kalkon total. TOTAL CHALCONE CONTENT IN THE ETHANOL EXTRACT OF ASHITABA STEMS (Angelica keiskei Koidzumi)  ABSTRACT Ashitaba  (Angelica  keiskei)  is  a  medicinal  plant  that  has  been  proven  to  possess antioxidant activity equal to that of vitamin E. This antioxidant activity usually belongs to  the  plant?s  secondary  metabolites,  e.g.  flavonoids.  The  ashitaba  stem  contains chalcone compounds, e.g. xantoangelol (XAG) and 4-hydroxiderricin (4-HD), which are categorized as open C-ring flavonoids. The purpose of this study was to determine total chalcone content (calculated as XAG) in ethanol extract of ashitaba stem. Ashitaba plant was obtained from Mount Rinjani, Lombok, and was identified at School of Biology Sciences and Technology, ITB. The total chalcone was determined by using standard addition spectrophotometric method. Result showed that the ethanol extract of ashitaba stem contain flavonoids, alkaloids polyphenol, and tannins, whereas the dried extract of ashitaba stem contained 0.836% w/w of total chalcone calculated as XAG. Keywords: Ashitaba, antioxidant, xantoangelol, 4-hydroxiderricin, total chalcone.
PENETAPAN KADAR DEHIDRODIISOEUGENOL DALAM TABLET EKSTRAK PALA DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Sulistyaningsih, Dwi Lestari; Mutakin, Mutakin; Lestari, Keri; Levita, Jutti
Indonesian Journal of Applied Sciences Vol 4, No 2 (2014)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2143.441 KB) | DOI: 10.24198/.v4i2.16802

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AbstrakPenetapan kadar dehidrodiisoeugenol dalam tablet ekstrak pala dapat menggunakan KCKT fase terbalik dengan kolom C18, fase gerak metanol dan air dengan perbandingan 73:27 menggunakan detektor ultraviolet pada panjang gelombang 282 nm dan waktu retensi dehidrodiisoeugenol 13,79 menit. Perolehan kembali dehidrodiisoeugenol 100,14%±0,55% Batas deteksi 0,892 ?g/ml dan batas kuantisasi 2,97 ?g/ml. Kadar tablet ekstrak pala 300 mg mengandung dehidrodiisoeugenol sebanyak 0,818 mg.Kata kunci : dehidrodiisoeugenol, validasi, KCKT
A REVIEW: IS GINGER (ZINGIBER OFFICINALE VAR. ROSCOE) POTENTIAL FOR FUTURE PHYTOMEDICINE? Syafitri, Dina Mulyana; Levita, Jutti; Mutakin, Mutakin; Diantini, Ajeng
Indonesian Journal of Applied Sciences Vol 8, No 1 (2018)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.755 KB) | DOI: 10.24198/ijas.v8i1.16466

Abstract

AbstractZingiber officinale Roscoe (red:ginger), is one of the most widely consumed medicinal herbs in the world has been widely used to treat various disorders in Indonesia. There are three known types of ginger: giant ginger or white ginger (Zingiber officinale var. Roscoe), small white ginger or ginger emprit (Zingiber officinale var. Amarum), and red ginger (Zingiber officinale var. Rubrum). The main components of ginger rhizome are carbohydrates, lipids, essential oils, terpenes, and phenol compounds such as gingerol (23-25%) and shogaol (18-25%). Many studies had confirmed benefical effects of ginger against inflammation, free radicals, diabetes melitus, bacterial infection, cancer, nausea, etc. Z. officinale var. rubrum is considered a safe herbal remedy with only slight and not significant adverse effects. This plant is potential fur future phytomedicine, however it needs further explorations on its clinical studies in humans is expected, especially the efficacy and safety of the risk of side effects.
Analisis Mikroba, Timbal, Kadmium, dan Raksa pada Es Balok dan Bahan Pembuatannya Andriany, Dinny; Levita, Jutti; Warya, Sohadi; Maelaningsih, Firdha S
Indonesian Journal of Pharmaceutical Science and Technology Vol 1, No 2 (2014)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.672 KB) | DOI: 10.15416/ijpst.v1i2.7511

Abstract

Telah dilakukan penetapan kadar timbal, kadmium, dan raksa pada sampel es balok dan sumber air bahan baku pembuatan es balok yang berasal dari salah satu pabrik di Bandung menggunakan metode spektrofotometri serapan atom tanpa nyala. Penetapan kadar dilakukan berdasarkan persyaratan air minum yang terdapat dalam Permenkes RI No. 492/MENKES/PER/VI/2010. Hasil penelitian menunjukkan bahwa cemaran logam terbanyak yaitu es balok dari depot. Kadar timbal tertinggi dalam es balok berasal dari depot adalah 15,5 ppb dan kadar kadmium tertinggi adalah 11 ppb, sedangkan kadar timbal tertinggi pada air bahan baku adalah 4,32  ppb dan 5,77 ppb pada es balok, dan kadar kadmium tertinggi pada air bahan baku adalah 1,85 ppb, dan pada es balok sebesar 0,88 ppb. Kadar raksa tertinggi untuk air bahan baku dan es balok adalah 0,06 ppb. Dari hasil tersebut diketahui bahwa ketiga logam yang diperiksa tidak melebihi batas yang ditetapkan yaitu 10 ppb untuk timbal, 3 ppb untuk kadmium, dan 1 ppb untuk raksa. Kata kunci: Es balok, kadmium, raksa, timbal
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT Levita, Jutti
INDONESIAN JOURNAL OF PHARMACY Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (992.952 KB) | DOI: 10.14499/indonesianjpharm25iss3pp138

Abstract

Andrographis paniculata or sambiloto is one of the most widely used medicinal herbs in Indonesia. The main bioactive chemical constituent, andrographolide, has been reported to have various pharmacological activities. Besides its function for medical purposes, the sambiloto herbs infusion is frequently taken to maintain health. This study was conducted to determine the bioavailability of sambiloto herbs infusion in rabbit plasma, stomach, and liver, calculated as total andrographolide. Fourteen male New Zealand white rabbits were used in this study. Sambiloto herbs infusion were administered orally at the dose 7.04mL/kg body weight to each rabbit. Blood samples were taken at intervals 0.0; 0.5; 1.5; 2.0; 3.0; and 5.0h after infusion administration. Sambiloto herbs infusion, which are calculated as andrographolide, levels in plasma, stomach, and liver were analyzed by high performance liquid chromatography using C-18 column as stationary phase and a mixture of methanol-double distilled water (60:40) as mobile phase. Bioavailability parameters obtained were Cmax 0.5549µg/mL (in stomach), 0.2136µg/mL (in plasma), 0.0051µg/mL (in liver); while tmax 1h (in stomach), 1.5h (in plasma), 2h (in liver); and AUC 1.7451µg.h/mL (in stomach), 0.434µg.h/mL (in plasma), 0.0038µg.h/mL (in liver). These data showed that in healthy animals, sambiloto herbs infusion was fastly absorbed from the stomach, distributed in the circulation system, and metabolized in the liver, in subsequent process. Sambiloto herbs infusion showed good bioavailability in rabbit. 
Radioiodination of andrographolide and its biodistribution in mice for inflammatory tracer Levita, Jutti; A., Cahya Nova; Nawawi, As’ari; Ibrahim, Slamet
INDONESIAN JOURNAL OF PHARMACY Vol 21 No 4, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.965 KB) | DOI: 10.14499/indonesianjpharm0iss0pp258-265

Abstract

Andrographolide,  a  bioactive  component  of  Andrographis  paniculata (Burm.F)  Nees,  is  the  major  lactone  diterpenoidal  bicyclic  constituent  in  this plant  which  has  proven  to  exert  anti-inflammatory  activity  in  vitro  which  was occurred via  several mechanism, e.g inhibition of inducible nitric oxide synthase (iNOS),  inhibition  of  radical  oxygen  species,  and  inhibition  of  NF-kappaB activation. The labeling with radionuclide is often used for therapy, detection and quantification of metabolites in the body. Even though the metabolites are very low  in  concentration  they  can  be  detected  by  the  energy  they  emitted. Radionuclide  can  be  used as  radiotracer  to  detect  whether  drug  really interacts with  its  target.  The  objective  of  this  research  is  to  synthesize 131I-labelled andrographolide  and  to  study  its  biodistribution  in  mice  to  understand  the location  of  its  organ  target.  Indirect  radioiodination  of  andrographolide  wascarried  out  by  using  bromine  as  the  leaving  group  and  followed  by  fast iodination  at  40oC,  yielded  72.6  %  purity  of  the  labeled  compound.  Iodination was occurred through proton substitution at C-12. Then the andrographolide-131I was  injected  into  lateral  vein  of  mice’s  tail  to  study  its  biodistribution.  The compound  was  distributed  in  all organs  with the  highest  accumulation  occurred in  the  stomach  (16.87  %/gram  organ).  The  result  showed  that  inducing  the animals  with  LPS  caused  inflammation  in  the  stomach  and  increased  the production  of  prostaglandin  as  proven  by  the  distribution  of  the  radioligand  in that organ.Key words: andrographolide, radioiodination, anti-inflammatory, biodistribution
The determination of quercetin in Plectranthus scutellarioides(L.) R.Br. leaves extract and its In SilicoStudy on Histamine H4 Receptor Moektiwardoyo, Moelyono; Levita, Jutti; Sidiq, Syafrudin Purnama; Ahmad, Khoziah; Mustarichie, Resmi; Subarnas, Anas; ., Supriyatna
INDONESIAN JOURNAL OF PHARMACY Vol 22 No 3, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.578 KB) | DOI: 10.14499/indonesianjpharm0iss0pp191-196

Abstract

Plectranthus  scutellarioides  (L.)  R.Br.,  or  jawer  kotok,  Family  Lamiaceae, grows  widely  in  Indonesia,  and  has  a  long  history  of  therapeutic  usage  in Indonesian traditional jamuto cure various diseases. The brownish purple leaves of  Plecranthus  contain  alkaloids,  saponin,  flavonoids,  tannin,  volatile  oils,  and quercetin  which  has  been  proven  to  exert  antiinflammatory  activity.  In  this research,  a  determination  of  quercetin  in  Plecranthus  leaves  extract  was performed and followed by a study of its interaction with histamine H4 receptor to  understand its  anti-inflammatory  activity.  The  dry  leaves  were  macerated by using  a  mixture  of  methanol  and  water  (1:1)  for  48  hours  and  the  solvent  was evaporated  at  low  temperature  (40-50oC).  Analysis  of  quercetin  in  the  extract was performed by using reversed-phase HPLC method LC-10AT VP (Shimadzu), Atlantis  Hilicsilica  C18  (Waters®)  150  mm  x  4.6  mm,  5  µm  as  stationary  phase and  a  mixture  of  acetonitrile,  phosphoric  acid,  and methanol  (40:50:10),  flow rate 0.8 mL/minute.  In silicostudy of quercetin with histamine H4 receptor was performed by using AutoDock Tools 3.0.5. Histamine H4 receptor (H4R) belongs to  G  protein-coupled  receptors  which  is  involved  in arthritis,  asthma,  and inflammations.  The  3D  structure  model  of  H4R  was  built  by  using  MODELLER 9v7.  Quercetin  contained  in  Plecranthus  leaves  extract  was  0.05  %.  This compound interacted with H4R viahydrogen bond formation with Lys158 (2.006 Å)  and  Glu182  (2.048  Å),  and  van  der  Waals  interaction  with  Trp90,  Leu91, Asp94, Tyr95, Phe168, Thr178, Ser179, Tyr319, Phe344, and Tyr340, therefore Plecranthus  leaves  extract  might  have  a  chance  to  be  used  as  histamine  H4 receptor inhibitor.Key  words  :   histamine  H4  receptor,  in  silico  study,  Plecranthus  leaves,  Plectranthus scutellarioides, quercetin
Formulasi dan Uji Stabilitas Tetes Mata Sulfasetamida Abdassah, Marline; Noviardani, Tenri; Levita, Jutti; Suherman, Shelvy E
Indonesian Journal of Pharmaceutical Science and Technology Vol 2, No 1 (2015)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.024 KB) | DOI: 10.15416/ijpst.v2i1.7808

Abstract

 Tetes mata sulfasetamidayang mengandung natrium sulfasetamida 10%, 15%, dan 30% telah dibuat dan disterilkan. Metode sterilisasi yang digunakan adalah uap air mengalir 98-100 oC, penyaring bakteri, dan autoklaf 120-121 oC selama 15 menit. Tujuan penelitian ini adalah mendapatkan sediaan yang paling stabil selama penyimpanan 28 hari. Semua sediaan diamati kejernihan, pH dan konsentrasi natrium sulfasetamida.Semua sediaan mengalami kenaikan pH. Kekeruhan terjadi pada sediaan yang disterilkan dengan autoklaf. Sediaan paling stabil adalah tetes mata yang mengandung natrium sulfasetamid 10% yang disterilisasi dengan penyaring bakteri.
Co-Authors Abdul Mutalib ABNAZ, ZAHRA DZAKIRAH Ade Zuhrotun, Ade Ahmad Muhtadi Ajeng Diantini, Ajeng Amalia, Riezki Anas Subarnas Anggraeni, Sra R. Anggraeni, Sra R. Anis Khoirunisa, Anis As’ari Nawawi, As’ari Aulifa, Diah Lia Azinar, Nurdjanah Cahya Nova A., Cahya Nova Dinny Andriany, Dinny Dolih Gozali, Dolih Driyanti Rahayu, Driyanti Eli Halimah, Eli Ellin Febrina, Ellin Fara Warhead, Fara Fattah, Miswar Fattah, Miswar Febrina Amelia Saputri, Febrina Amelia Fikri, Fauzan Fikri, Fauzan Firdha S Maelaningsih, Firdha S HIDAYAT, IYAN RIFKY Ida Musfiroh, Ida IKRIMA, KIKI Khoziah Ahmad, Khoziah Kolina, Jennifer Kolina, Jennifer Larasati, Batari A. Lesmana, Ronny Lestari, Keri Marline Abdassah, Marline Masripah Masripah Megantara, Sandra MEGANTARA, SANDRA Moelyono Moektiwardoyo, Moelyono Monika Novryanti, Monika Mutakin Mutakin Nawawi, As'ari Nawawi, Asari Nuraeni Nuraeni Nyi Mekar Saptarini Pebiansyah, Anisa Prabowo, Yudha Prisdiany, Yulin Puspitasari, Irma Melyani Puspitasari, Irma Meylani RAHMAH, HILMA AWALIA Resmi Mustarichie, Resmi Rini Hendriani, Rini Rizal, Mochamad SALIM, STEFANNY AGNES Saptarini, Nyi M. Saptarini, Nyi M. SEMBIRING, DINA Shan, Kee P. Shan, Kee P. Shelvy E Suherman, Shelvy E Sitepu, Rini Daud Slamet Ibrahim Sofa D. Alfian, Sofa D. Sohadi Warya Sri Adi Sumiwi Steffi Liem, Steffi Sukmana, Indriyanti R. Sukmana, Indriyanti R. Sulistyaningsih, Dwi Lestari Sumiwi, Sri A. Sumiwi, Sri A. Supriyatna ., Supriyatna Supu, Rini Daud Syafitri, Dina Mulyana Syafrudin Purnama Sidiq, Syafrudin Purnama Tenri Noviardani, Tenri TIANA MILANDA Tina Rostinawati, Tina Wicaksono, Imam A. Wijaya, Chyntia R. Wijaya, Chyntia R. YOGIYANTO Yogiyanto, YOGIYANTO Yula, Renyiska Yuli Andriani ZAIN, DICHY NURYADIN Zelika Mega Ramadhania, Zelika Mega