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MIKROENKAPSULASI PIGMEN DARI KUBIS MERAH: STUDI INTENSITAS WARNA DAN AKTIVITAS ANTIOKSIDAN Lukitasari, Diah Mustika; Indrawati, Renny; Chandra, Rosita Dwi; Heriyanto, _; Limantara, Leenawaty
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 1 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.61 KB) | DOI: 10.6066/jtip.2017.28.1.1

Abstract

Utilization of artificial food colorants has obtained particular concern for long time consumption. Red cabbage (Brassica oleracea L. var. capitata forma rubra L.) was extracted to produce a candidate for natural food colorant through a one-step physical extraction and microencapsulation. The color strength was determined by measuring: (1) tinctorial strength, (2) color degradation kinetics at various pH, and (3) thermostability. Yield level and antioxidant activity were enclosed as supporting data. The results showed that extract of red cabbage exhibited vivid red until green color at various pH and was nearly stable at pH 2 and 3, meanwhile its thermostability was fairly good at pH 3, 4, 8, and 9. The encapsulated red cabbage extract produced high color intensity at pH 2 for red color and pH 9 for blue color with a yield level of  5.53%. It also showed antioxidant activity with IC50 value of 725.65±0.086 ppm in methanol solvent and 258.25±0.097 ppm in water-methanol solvent.
EFEK BETA KAROTEN DAN AGREGASI KLOROFIL PADA FOTOSTABILITAS KLOROFIL A DALAM PELARUT ASETON Costa, Junet. F. da; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.132 KB) | DOI: 10.31258/jnat.11.2.115-123

Abstract

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive,immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light.Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e? 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene.Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.
FOTOPROTEKSI KURKUMIN TERHADAP β-KAROTEN PADA BERBAGAI NISBAH MOLAR SERTA AKTIVITAS ANTIOKSIDANNYA Natalina, Elly; Rahayu, Puji; Sulistyowati, Sulistyowati; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.206 KB) | DOI: 10.31258/jnat.12.1.1-8

Abstract

Curcumin and ?-carotene are two kinds of pigments serve as antitumor, anticancer and antioxidant agents. Theantioxidant activity of curcumin is stronger and more stable as compared to ?-carotene, so it can protect ?-carotene degradation from light and oxygen. Therefore curcumin has higher antioxidant activity as well. Theresearch was conducted by mixing curcumin and ?-carotene in several molarity ratios followed by iradiation usingvolpi lamp for 0, 10, 30, 60, and 90 minutes. The result shows that curcumin has photoprotection effect because itcan protect photooxidation of ?-carotene. Mixing of curcumin and ?-carotene 1:1 was the most stable ratio forprotecting ?-carotene.
ANALISIS KOMPOSISI DAN KANDUNGAN KAROTENOID TOTAL DAN VITAMIN A FRAKSI CAIR DAN PADAT MINYAK SAWIT KASAR (CPO) MENGGUNAKAN KCKT DETEKTOR PDA Syahputra, M. Rio; Karwur, Ferry F; Limantara, Leenawaty
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.953 KB) | DOI: 10.31258/jnat.10.2.89-97

Abstract

This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition ofcarotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then theresult was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974).The type and composition of both phases of CPO were determined by Choo?s method (1994) by using HPLC withPhoto Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. Theresult shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 ?g/g (liquid), 352 ± 17.7 ?g/g(solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositionsof both phases of CPO were dominated by ?- and ?-carotenes. The result shows that ?- and ?-carotenes preparedby saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are28.14% and 59.44%. The result for solid phase shows that ?- and ?-carotenes by saponification are 25.89% and60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantagesof using HPLC with PDA detector for identification and analysis of type and carotenoid composition.
OPTIMASI PROSES EKSTRAKSI FUKOSANTIN RUMPUT LAUT COKLAT PADINA AUSTRALIS HAUCK MENGGUNAKAN PELARUT ORGANIK POLAR Limantara, Leenawaty; Heriyanto, Heriyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.42 KB) | DOI: 10.14710/ik.ijms.16.2.86-94

Abstract

Rumput laut coklat merupakan salah satu sumber daya alam laut yang belum dimanfaatkan secara optimal oleh masyarakat Indonesia. Warna rumput laut coklat berasal dari salah satu pigmen dominan yang terdapat dalam rumput laut ini yaitu fukosantin, yang bermanfaat sebagai anti-kanker dan anti-obesitas. Penelitian mengenai proses optimasi ekstraksi fukosantin pada rumput laut coklat belum banyak dilakukan, sehingga penelitian ini bertujuan  untuk  menentukan  pelarut  organik  polar  yang  dapat  mengekstrak  fukosantin  secara  optimal. Fukosantin diekstraksi dari Padina australis Hauck menggunakan lima jenis pelarut organik polar, yaitu: aseton, asetonitril,  dimetil  sulfoksida (DMSO),  etanol  dan  metanol.  Berdasarkan  hasil  analisis  spektrometri,  nilai absorbansi pada panjang gelombang serapan maksimum (?mak) fukosantin dari spektra serapan ekstrak kasar pigmen, Padina australis Hauck yang diekstraksi dengan pelarut metanol memiliki nilai absorbansi relatif tinggi yaitu 0,9338 hampir sama ketika DMSO dan etanol digunakan sebagai pelarut ekstraksi. Hasil ini diperkuat oleh hasil analisis kandungan fukosantin dengan metode Kromatografi Cair Kinerja Tinggi (KCKT) dimana kandungan fukosantin mencapai 2,6049 mg/g berat kering dengan pelarut metanol dan kandungan fukosantin ini lebih tinggi 1,08-1,71 kali dibandingkan menggunakan pelarut organik polar lainnya. Kemurnian fukosantin dapat ditentukan berdasarkan nilai persentase luas puncak fukosantin (isomer cis dan trans fukosantin) terhadap luas dari seluruh puncak pigmen yang dapat dipisahkan pada kromatogram KCKT. Pelarut metanol memiliki nilai persentase luas puncak fukosantin relatif tinggi yaitu 60,11%. Berdasarkan hasil penelitian ini maka metanol merupakan pelarut yang terbaik untuk mengekstrak fukosantin dari Padina australis Hauck. Kata kunci: pelarut organik polar, ekstraksi, kandungan fuksosantin, Padina australis Hauck Brown seaweed, is one of marine natural resources, grows naturally and abundantly at Indonesian coastal waters, and yet Indonesians have not utilized it optimally. The color of brown seaweed ascribes to one kind of dominant pigments that this seaweed contains, namely fucoxanthin. Fucoxanthin has potent beneficial effects on human  health,  such  as  anti-cancer  and  anti-obesity  properties.  However,  the  research  concerning  the optimization of fucoxanthin extraction on brown seaweed has not been much done. Therefore, the purpose of this research is to determine the polar-organic solvent that can optimally extract fucoxanthin. Fucoxanthin was extracted from Padina australis Hauck by using various polar-organic solvents (such as acetone, acentonitrile, dimethyl sulfoxide (DMSO), ethanol and methanol). The result of spectrometry analysis, absorbance values at maximum absorption wavelength (?mak) of fucoxanthin from absorption spectra of pigment-crude extracts, showed that Padina australis Hauck extracted by methanol solvent has a relatively high absorbance value, i.e. 0.9338. This absorbance value was almost of the same value when DMSO and ethanol solvents are used as extraction solvent. This result supported the result of fucoxanthin content analysis by High Performance Liquid Chromatography (HPLC), where fucoxantin content was 2.6049 mg/g dry weight and this fucoxanthin content is 1.08-1.71 times higher when another polar-organic solvent was applied. The purity of fucoxantin can be determined from area percentage of fucoxantin peaks (cis and trans isomers fucoxanthin) toward the area of all pigments peaks that could be separated on HPLC chromatogram. Methanol solvent has a high area percentage value of 60.11%. Based on these experimental results, it can be claimed that methanol solvent is the best solvent for fucoxanthin extraction from brown seaweed Padina australis Hauck. Key words: polar-organic solvent, extraction, fucoxanthin content, Padina australis Hauck
ENKAPSULASI DENGAN KITOSAN-NATRIUM ALGINAT MENGGUNAKAN TEKNIK SPRAY-DRYING DAN FREEZE-DRYING Mulyadi, Naomi Megananda; Widyaningsih, Tri Dewanti; Wijayanti, Novita; Indrawati, Renny; Limantara, Leenawaty
Jurnal Pangan dan Agroindustri Vol 4, No 2 (2016)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya

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Abstract

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Perkembangan Sensitizer pada Terapi Fotodinamika Tumor dan Kanker Hingga Penuntunan Nanopartikel (Nanoparticulate Targeting) Dengan Antibodi Monoklonal INDRAWATI, RENNY; KARWUR, FERRY F; LIMANTARA, LEENAWATY
Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

World Health Organization (WHO) estimates both the number of patients and mortality rates due to cancer will continue to rise. Various researches were conducted in order to prevent and handle the cases of tumors and cancers, including the application of photosynthetic pigment molecules known as photodynamic therapy (PDT). Chlorophylls, the main pigment in photosynthesis, have an ability to capture light energy and control series of photobiology and photochemical processes1. In PDT, chlorophyll or its derivatives compounds act as the sensitizer which have energy excitation by light radiation (visible or near infra red), and generate some reactive oxygen species which triggers the death of cancer cells selectively (through apoptosis and / or necrosis pathway)2. Sensitizer compounds have been progressing from the first to third generation. The development of the third generation sensitizer was influenced by the advances of nanotechnology which lead to the improvement of PDT efficacy. The structure and size of nanoparticles can increase light absorption, and make the sensitizer accumulate in cancer tissues more specifically 3-4. Furthermore, nanoparticulatte targeting also interested to be studied because by conjugate functional groups, i.e. monoclonal antibody, on the sensitizer, it can improve the selectivity of therapy in targeting tumor and cancer tissues.
Pigmen Spirulina sebagai Senyawa Antikanker Pirenantyo, Prasetyandaru; Limantara, Leenawaty
Indonesian Journal of Cancer Vol 2, No 4 (2008): Oct - Dec 2008
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3164.34 KB)

Abstract

Spirulina, ganggang hijau biru yang berbentuk spiral, mengandung fitonutrisi yang cukup lengkap dan berpotensi sebagai agen antikanker yang efektif. Fikosianin, klorofil a, dan karotenoid adalah pigmen-pigmen yang dikandung Spirulina. Artikel ini merangkum berbagai penelitian in vivo dan ex vivo yang melaporkan ketiga pigmen tersebut merupakan antioksidan kuat. Bahkan, beberapa laporan mencatat bahwa turunan klorofil a juga berpotensi sebagai antimutagen, induktor apoptosis, dan fotosensitizer untuk PDT (Fotodinamika Terapi Kanker). PDT diharapkan memberikan harapan baru dalam terapi kanker. Namun demikian, tidak hanya pigmen yang berperan sebagai antioksidan dalam terapi penyembuhan kanker dengan Spirulina, nutrisi lain seperti asam lemak GLA (gamma linoleic acid), enzim superoksida dismutase (SOD), dan oligosakarida juga turut berperan sebagai senyawa antikanker. Spirulina dijual dalam bentuk suplemen golongan CAM (Complementary and Aternative Medicine). Terapi dengan mengkonsumsi spirulina cair, tablet, kapsul, ataupun bubuk secara teratur menawarkan sebuah jawaban bagi mereka yang sedang bergumul dengan kanker stadium awal jika ingin menghindari pembedahan, radioterapi, ataupun kemoterapi.vKata kunci: spirulina, fikosianin, klorofil a, karotenoid, antioksidan, complementary and alternative medicine
Potensi Astaxantin sebagai Senyawa Antikanker Pratiwi, Reny; Limantara, Leenawaty
Indonesian Journal of Cancer Vol 2, No 4 (2008): Oct - Dec 2008
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Astaxantin merupakan salah satu golongan karotenoid xantofil yang memiliki peran penting dalam menjaga kesehatan tubuh. Pada berbagai studi telah dibuktikan peran astaxantin dalam melindungi sel dan jaringan dari kerusakan terutama akibat stres oksidatif yang juga dapat memicu terjadinya kanker. Dalam tulisan ini akan dibahas mengenai potensi astaxantin sebagai senyawa antikanker.Kata Kunci: astaxantin, antioksidan, antikanker.
RAGAM METODE EKSTRAKSI KAROTENOID DARI SUMBER TUMBUHAN DALAM DEKADE TERAKHIR (TELAAH LITERATUR) Maleta, Hana Susanti; Indrawati, Renny; Limantara, Leenawaty; Brotosudarmo, Tatas Hardo Panintingjati
Jurnal Rekayasa Kimia & Lingkungan Vol 13, No 1 (2018): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.545 KB) | DOI: 10.23955/rkl.v13i1.10008

Abstract

Karotenoid adalah pigmen yang memberikan warna kuning, jingga hingga merah. Karotenoid merupakan pigmen pendamping klorofil atau zat hijau daun yang menjalankan fungsi penyerapan energi cahaya untuk fotosintesis. Sumber karotenoid utama adalah tumbuhan, yang selanjutnya dikonsumsi dan dimetabolisme atau terakumulasi dalam tubuh hewan.  Terdapat lebih dari 3000 jenis karotenoid, dan beberapa jenis telah diketahui memiliki manfaat bagi kesehatan. Beberapa studi menyebutkan karotenoid berfungsi sebagai antioksidan, antikanker, dan membantu memelihara kesehatan mata. Ekstrak karotenoid telah diaplikasikan dalam berbagai produk pangan dan nutrasetikal. Dengan demikian, perkembangan teknologi metode ekstraksi karotenoid menjadi penting untuk diikuti. Ekstraksi karotenoid dilakukan dengan metode konvensional seperti maserasi ataupun modern seperti cairan superkritis, ultrasound asssisted extraction, enzimatik, pulsed electric field, dan pressurized liquid extraction. Sejumlah studi metode ekstraksi karotenoid dalam dekade terakhir disajikan dan dibahas dalam review berikut.