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ISOLASI DAN AKTIVITAS ANTIBAKTERI AKTINOMISETES ASAL TANAH GAMBUT RIAU Linda, Tetty Marta; Roza, Rodesia Mustika; Yuliati, Rola; Wahyuliyanti, Wahyuliyanti
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.074 KB) | DOI: 10.31258/jnat.10.1.18-23

Abstract

The aims of this study are to isolate actinomycetes from peat soil samples, to determine the ability of actinomycetes to inhibit the growth of Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negativebacteria (Escherichia coli and Pseudomonas sp.). A total of 14 actinomycetes strains were recovered from peat soil samples using pour plate method with Starch Casein Agar. The results showed that 11 isolates were active against B. subtilis, 8 isolates against S. aureus, 8 isolates were active against E. coli and 8 isolates againstPseudomonas sp. Two isolates (SM 1.3 and SM 1.6) were active against all bacterial targets.
Seleksi Aktinomisetes Penghasil Protease dari Tanah Gambut Desa Langkai, Siak, Riau LINDA, TETTY MARTA; MARTINA, ATRIA; FEBRIANTI, BERNADETA LENI; , HERLINDA; , TABRI
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Actinomycetes is well known as secondary metabolites producer and hence it has high pharmacological andcommercial interest. In this study, a total of 14 isolates of actinomycetes from peat soils in Langkai Village,Siak, Riau had been selected for their ability to produce protease. Extracellular protease from Actinomycetesisolates was characterized by incubating the isolate in Nutrient Agar media containing casein 1%. Screeningof actinomycetes isolates showed that five isolates had the ability to produce a clear zone with the value ofproteolytic index (IP) ranged from 5.73 to 11.15. Isolates L313 showed the highest IP value (11.15), as wellas the highest protease production (0.041 U/ml) in Nutrient Broth media containing casein 1% after 6 daysincubation at room temperature.Key words: actinomycetes, clear zone, peat soil, protease, proteolitic index
Uji Patogenisitas Fungi Entomopatogen Lokal Riau sebagai Agen Biokontrol Hama Rayap (Coptotermes curvignathus Holmgren) SINTAWATI, REGIANA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Biopesticide is a technique that can be used to the control pest population in the environment. Thepurpose of this research was to isolate fungi from the soil using baiting and cadaver technique andpathogenicity test. Isolates which had high germinating conidia was tested their patogenicity againstCoptotermes curvignathus. A total of 12 isolates were baited from soil and cadaver samples at palm oilplantation. The result showed eight isolates had high germinating conidia categories (˃ 80%). Thepathogenicity test of four isolates showed 100% mortality for RB.r01 and BK.r01; and KB.r01 andNK.r01were 72% and 67% respectively. Isolates capability against termite was not significantly different,but it was significantly different from control.Keywords : entomopathogenic fungi, pathogenicity, Coptotermes curvignathus, biocontrol
Seleksi dan Akivitas Enzim Selulase Aktinomisetes Lokal Riau pada Media Lignoselulosa Ampas Tebu PESRITA, ANALIA; LINDA, TETTY MARTA; DEVI, SILVERA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Ampas tebu merupakan limbah pertanian yang mengandung selulosa, hemiselulosa, dan lignin sehingga dapat digunakan sebagai media untuk menumbuhkan mikroorganisme yang mampu menghasilkan enzim selulase, xylanase dan ligninase. Tujuan dari penelitian ini adalah untuk melihat kemampuan isolat aktinomisetes lokal dalam menghidrolisis substrat pada medium CMC (Carboxilmetil cellulose ) dan ampas tebu. Sebanyak 13 isolat aktinomisetes lokal diseleksi dan diuji aktivitas enzim selulase yang dihasilkannya pada medium CMC dan medium ampas tebu. Seleksi dilakukan secara kualitatif berdasarkan rasio zona bening yang terbentuk, sedangkan isolat dengan rasio tertinggi akan diuji aktivitas selulase yang dihasilkannya secara kuantitatif menggunakan dengan metode DNS (Dinitro Salicylic Acid). Hasil yang diperoleh menunjukkan semua isolat mampu memperlihatkan selulolitik hidrolitik selulase baik pada medium CMC dan medium ampas tebu. Rasio zona bening pada medium ampas tebu lebih tinggi dari medium CMC yaitu sebesar 40,72 ± 0,95 yang diperlihatkan oleh isolat L223. Uji kuantitatif dari aktivitas selulase isolat L223 sebesar 35,30 x10-3 U/ml.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
SELECTION OF ACTINOMYCETES IN PRODUCING INDOLE ACETIC ACID AND EFFECT OF GERMINATION SEEDS RED PEPPER (CAPSICUM ANNUM L.) Anggraini, Yeyen Septia; Linda, Tetty Marta; Lestari, Wahyu
Biospecies Vol 11 No 2 (2018): Volume 11, No.2, July, 2018
Publisher : Universitas Jambi

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Abstract

Indole Acetic Acid (IAA)  is an important that could improve plant growth. Actinomycetes is one group of bacteria that could produce IAA. The purpose of this study was to examines the potential of actinomycetes from Siak, Riau Peatland in producing IAA the effects to red pepper (Capsicum annuum L.) germination. The result of this study was collected 21 isolates of actinomycetes which able only to produced  IAA in SCB (Starch Casein Broth) medium with tryptophan addition. The produce highest IAA in L225 isolate with concentration 37,70 ± 1,00 µg/ml by medium SCB with typtophan addition. 14 isolates gave respons for length of sprouts parameters and 20 isolates gave effect to the root length parameters for red pepper seeds with incubation for 15 day.
KEMAMPUAN ISOLAT TUNGGAL DAN KONSORSIUM AKTINOMISETES LOKAL RIAU DALAM MENDEGRADASI HIDROKARBON MINYAK BUMI Fanny, Novalita Dwi; Linda, Tetty Marta; Martina, Atria
BIO-SITE |Biologi dan Sains Terapan Vol 4 No 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.94 KB) | DOI: 10.22437/bs.v4i2.5691

Abstract

Pollution of crude oil on environmental can have an effect of nutrient uptake on plants and balance of ecosystems.   Biodegradation. is one way to reduce petroleum pollution . The purpose of this research are determine the potential growth of actinomycetes local Riau (L11 and L121) in crude oil hydrocarbons at concentrations of 0%, 2%, 5% and to know their ability to degrade crude oil hydrocarbons at concentrations of 5%. This research observed the growth of each actinomycetes local Riau and the percentage of crude oil degradation by a single isolate and a consortium with gravimetric method. The results showed that the growth of L11 and L121 total population was not significantly different at addition crude oil of 2% and 5% but significantly different wth 0% (without crude oil). Test degradation of crude oil using single isolate gave significantly different results for the consortium isolates. Percentage of degradation crude oil by L11 was 23.5%, L121 was 19.2% and consortium isolate (L11 + L121) was 31.9% with an incubation time of 10 days in a minimum medium salts. This actinomycetes local Riau can be developed as biodegradation agents for crude oil on soil and water.