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Journal : Jurnal Ilmu Pertanian Indonesia

Validasi Metode Analisis Kolesterol dalam Telur dengan HPLC-ELSD Lioe, Hanifah Nuryani; Setianingrum, Tika; Anggraeni, Ririn
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.23 KB)

Abstract

A method using high-performance liquid chomatography (HPLC) coupled with an evaporative light-scattering detector (ELSD) for the determination of cholesterol in egg was validated. A silica column and a binary mixture of hexane and isopropanol (90:10) as a mobile phase were used to separate cholesterol. Cholesterol was detected at 1.5 min using cholesterol standard and HPLC-ELSD condition: evaporation temperature 50 ºC, air pressure 2.2 bars, and flow rate of mobile phase 2 mL/min. A method linearity for the cholesterol analysis in egg as a sample matrix was obtained at a range of 50 to 3000 µg/g sample, with R2>0.990. Instrument detection limit and limit of quantitation were determined at 1.07 and 3.56 µg/mL, respectively. Recovery test results by spiking cholesterol standard in egg sample at low, medium, and high concentrations (50, 250 and 3000 µg/g ) were 122.13, 108.23, and 44.71%, respectively. Their corresponding repeatability values were 5.26, 4.29, and 10.11%. Method detection limit and intralab reproducibility (to analyze a sample) were observed at 2.30 µg/g and 0.04%. The method is valid for cholesterol analysis in egg at low and medium concentrations.
Kajian Risiko Aflatoksin M1 dalam Produk Formula untuk Bayi dan Anak Usia 0-36 Bulan Restiani, Yeni; Nuraida, Lilis; Lioe, Hanifah Nuryani
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.954 KB) | DOI: 10.18343/jipi.25.1.160

Abstract

Aflatoxin M1 (AFM1) is a carcinogenic compound found in milk-based products including infant and young children formula. The aim of this study was to determine the risk characterization of infants and young children to AFM1 through the consumption of formula products. For this purpose, the Estimated Daily Intake (EDI) for infants and young children was calculated by multiplying AFM1 concentration in 44 infant formulas, 53 advanced infant formulas, and 16 growing up formulas with consumption data. The concentration of AFM1 in these formula products were retrieved from food registration in Indonesian FDA from January 2015-June 2018. The average concentration of AFM1 in infant formula, advanced infant formulas, and growing up formula (lower bound-upper bound) were 0.0226-0.0335; 0.0418-0.0510; and 0.0038-0.0123 ng/g, respectively. The average EDI of AFM1 for infants and young children aged 0-6 months, 6-12 months, and 1-3 years based on individual consumption were 0.260-0.386, 0.282-0.343, 0.029-0.092 ng/kg body weight (BW)/day. The average EDI of AFM1 for infants and young children aged 0-6 months, 6-12 months, and 1-3 years based on the recommended consumption by food producer (lower bound-upper bound) were 0.403-0.598; 0.663-0.809; 0.031-0.098 ng/kgBW/day. The average hazard index (HI) values for infants aged 0-6 months and 6-12 months (lower bound-upper bound) were greater than 1 (one), i.e., 1.94-2.88 and 3.20-3.90, which indicates there is a health risk. However hazard index value for young children aged 1-3 years (lower bound-upper bound) were less than 1 (one), i.e., 0.15-0.47, which indicates a lower health risk. Keywords: advanced infant formula, aflatoxin M1, growing up formula, hazard index, infant formula
Analisis 3-Monokloro-1,2-Propanadiol (3-MCPD) Ester dalam Minyak Sawit dengan Instrumen Kromatografi Gas-Spektroskopi Massa Lioe, Hanifah Nuryani; Yuliana, Nancy Dewi; Indrasti, Dias; Regiyana, Yane; Putri, Cony Arisya
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 2 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1997.19 KB) | DOI: 10.18343/jipi.20.2.115

Abstract

A group of components called as 3-monochloropropane-1,2-diol (3-MCPD) esters has been found in some vegetable oils, particularly palm oil. Analysis of these components needs GC-MS instrument to reach a low level detection, parts per billion or ppb level. An effort to validate the 3-MCPD esters analysis by GC-MS method (Weiβhaar method) using phenyl boronic acid (PBA) as a derivatizing agent and isotopic internal standard (IS) 3-MCPD-D5 followed by the determination of 3-MCPD in commercial palm oils has been conducted. The analytical results showed the instrumental performance as follows: instrument linearity for the analysis of 3-MCPD standard solutions containing IS 2.0 μg/mL was obtained at a concentration range of 0.25-7.50 μg/mL test solution with R2 higher than 0.990, precision of 3-MCPD as well as IS retention time was less than 2.0% which was acceptable according to JECFA guidance. Instrument detection limit (LOD) and limit of quantitation (LOQ) were read from GC-MS based on the linearity experiment, i.e. 0.17 and 0.59 μg/mL solution, respectively. Method linearity by using palm oil as a matrix also showed R2 higher than 0.990. Recovery test result at 40 μg/g gave an accuracy at 83.29 ± 7.12% which is in the range of AOAC standard (80-110%) and precision at 5.38% (less than RSDHORWITZ). Analysis of 14 palm oils from local market showed 100% of the sampels were positive to contain 3-MCPD esters. 3-MCPD esters in palm oil were found at a concentration range of 8.15-58.14 µg 3-MCPD/g sample.