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POTENSI KOMBINASI BAKTERI DAN JAMUR SELULOLITIK PADA FERMENTASI BEKATUL TERHADAP KANDUNGAN SERAT KASAR DAN PROTEIN KASAR Lokapirnasari, Widya Paramita; Setiawan, Agus; Prawesthirini, Soetji
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.827 KB) | DOI: 10.21059/buletinpeternak.v39i3.7985

Abstract

Penelitian ini bertujuan untuk mengetahui kandungan serat kasar dan protein kasar pada bekatul padi yang difermentasi dengan bakteri selulolitik dan jamur selulolitik. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola searah dengan sepuluh perlakuan dan masing-masing perlakuan dilakukan 3 kali ulangan. Ke sepuluh perlakuan tersebut adalah P0: 250 g bekatul + 3% tetes + Bakteri (B) 0% + Jamur (J) 0%; P1: 250 g bekatul + 3% tetes +B.10% + J.10%; P2: 250 g bekatul + 3% tetes + B.20% + J.10%; P3: 250 g bekatul + 3% tetes + B.30% + J.10%; P4: 250 g bekatul + 3% tetes + B.10% + J.20%; P5: 250 g bekatul + 3% tetes + B.20% + J.20%; P6: 250 g bekatul + 3% tetes + B.30% + J.20%; P7: 250 g bekatul + 3% tetes + B.10% + J.30%; P8: 250 g bekatul + 3% tetes + B.20% + J.30%; P9: 250 gbekatul + 3% tetes + B.30% + J.30%. Setelah dilakukan fermentasi selama 7 hari, dilakukan analisis proksimat. Data yang diperoleh selanjutnya diuji dengan analisis variansi, dilanjutkan dengan uji Duncan?s Multiple Range Test. Hasil penelitian menunjukkan bahwa bakteri selulolitik dan jamur selulolitik dapatmenurunkan kandungan serat kasar pada bekatul pada perlakuan P2 (28,96%), P3 (29,34%), P8 (29,53%), P4 (29,65%), P7 (30,23%), P6 (30,37%), P9 (30,58%) yang berbeda nyata (P<0,05) dengan perlakuan P5 (31,10%), P1 (31,98%), P0 (34,1 1%), serta dapat meningkatkan kandungan protein kasar pada perlakuan P2 (13,97%), P5 (12,87%), P3 (12,84%), P8 (12,74%) yang berbeda (P<0,05) dengan perlakuan P7 (12,71 %), P6 (12,44%), P4 (12,36%), P9 (12,27%), P1 (12,25%), P0 (10,9%).(Kata kunci: Acidothermus cellulolyticus, Aspergillus terreus, Bekatul, Fermentasi, Protein kasar, Serat kasar)
SEKUENSING 16S DNA BAKTERI SELULOLITIK ASAL LIMBAH CAIRAN RUMEN SAPI PERANAKAN ONGOLE (SEQUENCING OF 16S DNA OF CELLULOLYTIC BACTERIA FROM BOVINE RUMEN FLUID WASTE ONGOLE CROSSBREED) Lokapirnasari, Widya Paramita; Sahidu, Adriana Monica; Nurhajati, Tri; Supranianondo, Koesnoto; Yulianto, Andreas Berny
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.233 KB) | DOI: 10.19087/jveteriner.2017.18.1.76

Abstract

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase&nbsp;with primer forward PB36 5?-AGR GTT TGA TCM TGG CTC AG-3? and primer reverse PB38 5?-GMT ACCTTG TTA CGA CTT-3? for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5?-AGR GTT TGA TCM TGG CTC AG-3? dan primer reverse PB38 5?-GMT ACC TTG TTA CGA CTT-3? yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.
UJI AKTIVITAS PERTUMBUHAN ENTEROBACTER CLOACAE SELULOLITIK AEROB RUMEN-1 ISOLAT ASAL LIMBAH CAIRAN RUMEN SAPI PERANAKAN ONGOLE (GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAE SAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CRO Nurhajati, Tri; Soepranianondo, Koesnoto; Lokapirnasari, Widya Paramita
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.273 KB)

Abstract

This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask. Culturesuspensions were incubated in a shakerincubator (37°C, 120 rpm). Optical density was measured at ë 600 nm by taking as much as 1 mLsampling with interval of two hours for 24 hours (hour 0; 2; 4; 6; 10; 12; 14; 16; 18; 20; 22; 24). The firstsampling was done at 0thhour and continued until OD values ?showed a clear decline. Optical density wasmeasured with a UV-Vis spectrophotometer at wave length ë 600 nm. Growth curve was obtained from theresult of absorbance measurement on the time. Optimum growth production of E.cloacae SAR 1 occurredat the 12thhoursof incubation, optimum temperature of 35°C and optimum pH 6.
Potensi Bakteri Lactococcus sp. dan Lactobacillus sp. untuk Peningkatan Kualitas Limbah Kulit Kacang Sebagai Alternatif Bahan Pakan [Potential of Lactococcus sp. and Lactobacillus sp. Bacteria for Quality Improvement of Peanut Peel Waste as Alternative Feed Ingredients] Lokapirnasari, Widya Paramita; Widodo, Oky Setyo; Koestanti, Emy
Jurnal Ilmiah Perikanan dan Kelautan Vol 10, No 1 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.226 KB) | DOI: 10.20473/jipk.v10i1.8547

Abstract

                                                    AbstrakTujuan penelitian ini untuk menterminasi peningkatan kandungan nutrien dalam fermentasi makanan yang terdapat dari kulit kacang sebagai bahan pakan alternatif untuk ternak dan ikan. Penelitian ini terdiri dari tiga perlakuan dan lima replikasi P0: kulit kacang+isolat 0%; P1: kulit kacang+1% Lactococus sp. ; P2 : kulit kacang+1% Lactobacillus sp. Proses fermentasi dilakukan selama 5 hari dalam keadaan aerob. Hasil menunjukan bahwa terdapat berdedaan yang signifikan antara perlakuan (p<0,05). Penggunaan isolat Lactococcus sp. dan Lactobacillus sp. dapat meningkatkan kandungan nutrient dari limbah kulit kanang. Terdapat penurunan aerob mentah dari 3,80 sampai 7,70% terdapat peningkatan elestrak nitrogen bebas dari 3,925 sampai 4,38% terdapat peningkatan kandungan energi pencernaan 7.13% sampai 9.30%. berdasarkan hasil penelitian dapat disimpulkan bahwa pengguna 1% Lactococcus sp. dan 1% Lactococcus sp. dapat meningkatkan nilai nutrisi di limbah kulit kacang yang dapat digunakan sebagai alternatif bahan pakan ternak dan ikan.                                                   AbstractThis study aims to determine the increasing of nutrient content in the fermentation of feed stuff derived from peanut wastes as an alternative feed material for livestock and fish. The study consisted of three treatments and five replications, namely P0: peanut peel + 0% isolate, P1: peanut peel + 1% Lactococcus sp, P2: peanut peel +1% Lactobacillus sp. The fermentation process was carried out for 5 days under a facultative anaerobic condition. The results showed that there was a significant difference between treatments (p <0.05). The use of Lactococcus sp and Lactobacillus sp isolates can increase the nutritional content of peanut peel wastes. There was a decrease in crude fiber content (CF) of 3.80 to 7.70%, there was an increase of Nitrogen free extract (NFE) of 3.925 to 4.38%, there is an increase in digestible energy (DE) content of 7.13% to 9.30%, there was the increasing metabolizable energy (ME) of 7.13% to 9.12% compared to control. Based on the results it can be concluded that the using of 1% Lactococcus sp and 1% Lactobacillus sp. can increase nutritional value of peanut peel waste that can be used as an alternative ingredients of animal and fish.
PERFORMA PRODUKSI DAN ANALISIS USAHA PUYUH (COTURNIX COTURNIX JAPONICA) YANG DIBERI SUBSTITUSI BLACK SOLDIER FLY LARVAE (BSFL) PADA PAKAN KOMERSIL Hanifah, Fithria Nisa; Soepranianondo, Koesnoto; Soeharsono, Soeharsono; Arif, Anam Al; Lokapirnasari, Widya Paramita; Harijani, Nenny; Hadijah, Siti; Hutabarat, Mariana Ruth Theresia
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.078 KB) | DOI: 10.22146/jsv.49067

Abstract

Black Soldier Fly Larvae (BSFL) is an alternative protein source for livestock. This research aimed to know the effectiveness of BSFL for commercial feed substitution, related to production, productivity, and profitability in quail farm. This study used 80 quails which divided into 4 groups and each group consist of 20 quails. The treatments contained BSFL substitution with different composition, control group (P0) was given 100% commercial feed, (P1) was given a 5% BSFL substitution, (P2) was given a 10% BSFL substitution and (P3) was given a 20% BSFL substitution. Contribution margin (CM) analysis was used to determine the yields of the groups in this study. Results showed that the highest feed consumption rate is in P3, production (egg in total and weight), productivity and yields in P3 is the highest, although there's no difference between P0, P2 and P3 based on the analysis MANOVA.  BSFL substitution at 20% is recommended to the farmers to increase profits.
GAMBARAN SEL EOSINOFIL, MONOSIT, DAN BASOFIL SETELAH PEMBERIAN SPIRULINA PADA AYAM YANG DIINFEKSI VIRUS FLU BURUNG (OBSERVATION OF EOSINOPHILS, MONOCYTES, AND BASOPHILS AFTER TREATED WITH SPIRULINA IN CHICKENS THAT INFECTED WITH AVIAN INFLUENZA VIRUS) Lokapirnasari, Widya Paramita; Yulianto, Andreas Berny
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.458 KB)

Abstract

High Pathogenecity Avian Influenza (HPAI) viruses have high virulence and can frequently causesudden death on birds. The aims of this research was to know the role of Spirulina to a number ofmonocytes and lymphocytes in the blood of chickens which infected with the H5N1 virus. This researchconsisted of three levels of treatment in which each level given Spirulina 0%, 10%, 20% in the fresh wateralgae as drinking water. Each treatment consisted of seven replicates, and the treatment was done sincethe chickens at age 19 until 44 days ( for 25 days). Artificial infection of the chickens with the virus waschallenged by using AI (H5N1) 104 EID 50 (A/Ck/Indonesia/BL/03) with route to the respiratory tract (nosedrops) 0,1 mL starting on day 19. The results showed that there were a significant difference (p&lt;0.05) ontreatment that given Spirulina at doses of 0%, 10% and 20% for the number ofn monocytes, eosinophils,whereas no significant difference (p &gt; 0.05) was observed in basophils.
Pengaruh Subtitusi Tepung Biji Bunga Matahari (Helianthus Annuus L.) dalam Pakan Komersial dengan Konsentrasi Tertentu Terhadap Performa Ayam Pedaging Hermansyah, Beta; Lokapirnasari, Widya Paramita; Fikri, Faisal
Jurnal Medik Veteriner Vol 2, No 1 (2019): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.682 KB) | DOI: 10.20473/jmv.vol2.iss1.2019.7-12

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggantian tepung biji bunga matahari (Helianthus annuus L.) di pakan komersial dengan konsentrasi tertentu pada kinerja ayam pedaging. Penelitian ini menggunakan desain acak lengkap. Sampel terdiri dari 20 ekor ayam broiler dibagi secara acak menjadi 5 kelompok. Perlakuan adalah P1, P2, P3, dan P4 masing-masing CP511 pakan komersial yang mengandung tepung biji bunga matahari 25%, 30%, 35%, dan 40% serta kontrol tanpa substitusi P0 tepung biji bunga matahari. Variabel yang digunakan adalah konsumsi pakan, bobot akhir dan konversi pakan kemudian hasilnya dianalisis dengan Analisis Varians. Hasil yang diperoleh rata-rata nilai konsumsi pakan P0 adalah 79,76 gram, P1 adalah 77,90 gram, P2 adalah 77,35 gram, P3 adalah 77,98 gram, P4 adalah 77,58 gram. Hasil nilai rata-rata berat akhir 1545,5 gram yaitu P0, P1 adalah 1296,5 gram, P2 1325,75 gram, P3 1256 gram, P4 1203 gram. Hasil yang diperoleh dari nilai rata-rata konversi pakan adalah P0 1,37, P1 1,69, P2 1,61, P3 1,71, P4 1,88. Berdasarkan hasil dapat disimpulkan bahwa substitusi tepung biji bunga matahari dalam diet komersial tidak meningkatkan asupan pakan, berat badan dan dapat meningkatkan konversi pakan.
Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing maslachah, lilik; Suprayogi, Tri Wahyu; Lokapirnasari, Widya Paramita
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5209.205 KB) | DOI: 10.22146/jpkm.28219

Abstract

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product
Pemberdayaan Masyarakat Melalui Aplikasi Teknologi Inseminasi Buatan, Pengolahan Pakan , Biofarmaka dan Limbah dalam Upaya Pengembangan Sentra Kambing Maslachah, Lilik; Suprayogi, Tri Wahyu; Lokapirnasari, Widya Paramita
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 4, No 2 (2019): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5209.205 KB) | DOI: 10.22146/jpkm.28219

Abstract

ABSTRACT Community empowerment of the goat farming group in Kerek and Merakurak, Tuban aims to improve the knowledge of artificial insemination technology of goats, processing agricultural and plantation waste products for goat feed,processing and using medicinal plants and processing goat’s faeces waste to become environmentally-friendly fertilizer. Methods: observing the location, interviewing and discussing with the leader of the group to clarify the problems faced by goat farmers. Education given by seminar and training by demos of artificial insemination, complete feed processing, bio-pharmaceutical preparation and waste processing. Evaluation and monitoring the success of the sustainability program cooperating with local animal husbandry department for assistance by field operators serving on the area. The output of TTG is transformation of artificial insemination technology of goats. Knowledge and understanding of farmers about how to process complete feed for goats. Making bio pharmaceutical preparation independently. Production of environmentally-friendly fertilizer. Keywords: artificial insemination; bio-pharmaceutical; complete feed; waste product
PENGARUH PEMBERIAN PROBIOTIK Lactobacillus acidophilus DAN Bifidobacte-rium sp TERHADAP PERFORMA PRODUKSI AYAM PETELUR YANG DIINFEKSI Escherichia coli Huda, Khoirul; Lokapirnasari, Widya Paramita; Soeharsono, Soeharsono
Jurnal Ilmiah Teknosains Vol 5, No 1 (2019): JiTek
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jitek.v5i1.3459

Abstract

The purpose of this study is to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on feed consumption and Hen Day Production (HDP) laying hens which infected by Escherichia coli. Arround 120 layers at the age of 25 weeks are divided into six treatments. Each treatment consisted of factor a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The result of this study shows that giving probiotic has taken an effect (p<0,05) on the feed consumption and HDP. The infection factor takes effect (p <0.05) on HDP but it does not take effect (p>0,05) on the feed consumption. There is interaction among probiotic factor and infection (p>0.05) on HDP but it has no effect (p> 0.05) on feed consumption. The highest feed consumption is found in the giving of non-infectious probiotics that is 114.7 g and the highest HDP which is from the giving of non-infectious probiotics is 98.6 %. In conclusion, the giving of probiotic Lactobacillus acidophilus and Bifidobacterium sp are able to produce well perform product and the high profit.