Articles

SENSITIVITY OF PROPOLIS SOLUTIONS ON AEROMONAS HYDROPHILA BACTERIA Bako, Surandha; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Perikanan dan Kelautan - UNRI Vol 24, No 2 (2019)
Publisher : Universitas Riau

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HEMOTOLOGICAL OF CLARIAS GARIEPINUS THAT WERE GIVEN WITH VACCINE TO PREVENT MOTILE AEROMONAS SEPTICEMIA DISEASE Triyatna, Deny; Lukistyowati, Iesje; Riauwaty, Morina
Berkala Perikanan Terubuk Vol 47, No 3 (2019): NOVEMBER 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.128 KB) | DOI: 10.31258/terubuk.47.3.217-223

Abstract

The vaccine is one of the alternative to improve infection of the immunity system. The purpose of this research was to understand the hematological of (Clarias gariepinus) that were with given Aeromonas hydrophila vaccine. This research was conducted on 14 April until 2 June 2019. The method was an experimental method with a Completely Randomized Design (CRD) five treatments and three times replication. The treatments appliced were: Kn: (The fishes that were not given with vaccine and infected by Aeromonas hydrophila), Kp: (The fishes that were not given vaccine and was infected by Aeromonas hydrophila, P1: Soaked with vaccine dose 1 ml/10 L, P2: Soaked with vaccine dose 1 ml/10 L, P3: (Dipping with vaccine dose 1 ml/10 L. Dipping with the vaccine is done for 15 minutes. Clarias gariepinus at average 8-10 cm TL. After 23 day, the fishes were infected with Aeromonas hydrophila, the purpose of which was to determine imumunity of fish. The results show that the vaccine for 15 minutes is able to prevent the Motile Aeromonas Septicemia (MAS) disease. The were range total erythrocyte is 2,29×106 sel/mm3, hemoglobin 12,60 g/dl, Leukocytes Differensial (Lymphocytes 71,33%, Monocytes 16,66% and Neutrophils 12,00%) and Survival Rate 63,3%.
PENAMBAHAN TEPUNG BUAH MENGKUDU (MORINDA CITRIFOLIA L) PADA PAKAN TERHADAP PERUBAHAN AKTIVITAS FAGOSITOSIS, TOTAL ERITROSIT DAN HEMOGLOBIN IKAN NILA (OREOCHROMIS NILOTICUS) Yunita, Irma; Syawal, Henni; Lukistyowati, Iesje
Berkala Perikanan Terubuk Vol 44, No 3 (2016): November 2016
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.28 KB) | DOI: 10.31258/terubuk.44.3.38 - 45

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 This research was conducted from Mei to September 2015 in the Laboratory of Parasitic Diseases of Fish Faculty of Fisheries and Marine Science, University of Riau. The purpose of this reseach was to knowing the addition of flour noni (Morinda citrifolia L) on feed to change the phagocytic activity, total of erythrocytes and hemoglobin of tilapia (Oreochromis niloticus) infected Streptococcus iniae. This research used five treatments, 1) Kn: negative control (without a solution of addition flour noni (Morinda citrifolia L)  and without the infection of Streptococcus iniae.), 2) Kp: positive control (without giving a solution of addition of flour noni (Morinda citrifolia L) and the infection Streptococcus iniae) 3) P1: addition flour noni (Morinda citrifolia L) 3 g/kg feed) P2: addition flour noni (morinda citrifolia l) 6 g/kg feed) P3: addition flour noni (Morinda citrifolia L) 9 g/kg feed). The research shows that the addition of flour noni (Morinda citrifolia L) on feed do impact the condition of fish blood because visible from the phagocytic activity is ranged between 35,33-56,66%, total erythrocytes 1,23-2,20 x106 cell/mm3, and hemoglobin is ranged between 4,66-6,33 gr/%.
Differentiation Of Leukocytes Cat Fish (Mystus Nemurus) Fed Solutions Containing Curcuma (Curcuma Xanthorrhiza) Siagian, Juandi Riki Umbara; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The effect of containing curcuma (Curcuma xanthorrhiza Roxb) on diferentiation of leukocytes of Cat Fish (Mytus nemurus). The aim of the study is to find of diferentiation of leukocyte of Cat Fish (Mytus nemurus). The study was conducted from March to April 2014 in the Experimental Pond and Laboratory of Parasitic Diseases of Fish and Fisheries and Marine Sciences Faculty of the Riau University. The method used was experimental method using RAL with one factor with 4 level treatments and three repliations. the result indicate that the best differentiation of leukocytes in the P3 treatment is total leukocytes (96.538 cell/mm3), diferentiation of leukocytes (lymphocytes: 96,67%, monocytes: 1,67%, and neutrophils: 1,67%) and the best treament of survival rate is P2 and P3 with survival rate is 100%.Keywords: Differentiation, Leukocytes, Cat Fish, and Curcuma Solution
POTENSI KULIT BUAH MANGGIS (GARCINIA MANGOSTANA L) YANG DICAMPUR DALAM PAKAN UNTUK MENINGKATKAN KETAHANAN TUBUH DAN KELULUSHIDUPAN IKAN LELE DUMBO (CLARIAS GARIEPINUS) TERHADAP PENYAKIT MOTILE AEROMONAS SEPTICEMIA Lukistyowati, Iesje; Syatma, Mario
Berkala Perikanan Terubuk Vol 44, No 1 (2016): Februari 2016
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.671 KB) | DOI: 10.31258/terubuk.44.1.1 - 16

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This research was conducted on 12th May until 23rd July 2015 held at Parasite and Fish Disease Laboratory. The aims of this research was to determine the potency of mangosteen pericarp (G. mangostana L.) in feed to increase immunity of African catfish (C. gariepinus) on Motile Aeromonas Septicemia disease seen from the hematology parameters. The treatments were addition of mangosteen pericarp in feed with different concentration i.e : Kn (without mangosteen pericarp), Kp (without mangosteen pericarp and injected using A. hydrophila), P1 (addition mangosteen pericarp 2 g/kg of feed and injected using A. hydrophila), P2 (addition mangosteen pericarp 4 g/kg of feed and injected using A. hydrophila) and P3 (addition mangosteen pericarp 6 g/kg of feed and injected using A. hydrophila). Fish were maintained for 60 days. After 60 days treatment the fish were intramuscularly infected with A. hydrophila with density of 107cell/ml in a dose of 0,1 ml/fish. The best results showed that addition mangosteen pericarp in P3 treatment after challenged with A. hydrophila significantly different on haematocrit levels (22,94%), total leukocytes (104,83×103 cells/mm3), phagocytic activity (64,80%), and survival rate (100%). Total eritrocytes were not different significantly between treatment with 2,04×106 cells/mm3.
IDENTIFICATION OF LACTIC ACID BACTERIA FROM BIOFLOK TECHNOLOGY WHICH HAS BEEN GAVE MOLLASES ON RED TILAPIA (OREOCHROMIS SP.) AQUACULTURE Sitorus, Nelli Karolina; Lukistyowati, Iesje; Syawal, Henni; Putra, Iskandar
Berkala Perikanan Terubuk Vol 47, No 1 (2019): Februari 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.013 KB) | DOI: 10.31258/terubuk.47.1.83-92

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The aim of this research was to  determine the types of lactic acid bacteria from biofloc technology with the addition of mollase in red tilapia aquaculture containers and to determine the optimum growth of lactic acid bacteria at pH 2, 4, and 6, and also to observe the density of bacteria in the biofloc container water during maintenance. The methods used in this research are survey and experiments by taking flok from maintenance container and then identifying the lactic acid bacteria. The results of lactic acid bacteria inoculation obtained 16 isolates isolated from floc which can only grow at pH 4 and pH 6. Identification based on physical tests, chemical tests, and biochemical tests showed that the isolates were classified into Bacillus and Streptococcus. The amount of bacterial density water from containers of biofloc technology were given mollase during research reached the highest point in the third week, which averaged 109 CFU/mL.
DESCRIPTION OF LEUKOSIT PANGASIUS HYPOPHTHALMUS THAT IS INFECTED BY AEROMONAS HYDROPHILA AND TREATED WITH SOLUTION OF MANGIFERA INDICA L. Switzelin H, Dea; Lukistyowati, Iesje; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRAKThis research was conducted from October to December 2018 at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. This study aims to obtain the best dose from the solution of harumanis mango seeds (Mangifera indica L.) to treat catfish (P. hypophthalmus) infected with A. hydrophila seen from the picture of leukocytes. While the expected benefits are a solution of harumanis mango seeds can be used as a medicine to treat Motile Aeromonas Septicemia (MAS) disease. The method used in the research is the experimental method using a completely randomized design (CRD) with five levels of treatment, namely (Kn): Infected fish A. hydrophila and untreated mango seed solution, (Kp): Fish infected A. hydrophila are not treated with mango seed solution, (P1): Fish infected A. hydrophila were soaked with mango seed solution at a dose of 1600 ppm, (P2): Fish infected A. hydrophila were immersed in a manga seed solution at a dose of 1800 ppm (P3): Infected fish A. hydrophila is soaked in a solution of mango seeds at a dose of 2000 ppm. The leukocyte parameters measured were total leukocytes, leukocrit percentage, leukocyte differentiation and phagocytic activity.The results showed that the solution of harumanis mango seeds was influential in treating catfish infected with A. hydrophila, the dose of 2000 ppm mango seed solution was the best dose in the treatment of catfish infected with A. hydrophila seen from a total leukocyte of 7.37 x 104 cells / mm3, leukocrit levels of 2%, phagocytic index of 35.67%, and survival rate reached 80%. While the percentage of 81% lymphocytes, 10.67% monocytes and 8.33% neutrophils. The results of measurements of water quality during the study, namely temperature 28.2-28.50C, pH 5-6.7 dissolved oxygen 5-6.7 mg / L, and ammonia 0.02 mg / L. Keywords: Description of Leukosit, Pangasius hypophtalmus, Mangifera indica L., Aeromonas hydrophila 
HISTOPATHOLOGY OF Mystus nemurus LIVER THAT WERE FED WITH FEED ADDED WITH Psidium guajava AND Andrographis paniculata NESS S, Morina Riauwaty; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on June to August 2014 in the Laboratory of Parasitesand Diseases of Fisheris and Marine Science of Faculty Riau University Pekanbaru. Thepurpose of this research was to determine the structure of (Mystus nemurus) liver that werefed with feed added with Psidium guajava and Andrographis paniculata Ness. The methodused is an experimental using 3 treatments with 3 replicated. The treatment used in thisresearch was Kn (Fishes were not receive any treantent), Kp (Fishes were not receive anytreatment but were infected with A. hydrophila), P1 ( Fishes were fed with feed added with10 g of Psidium guajava and 10 g of Andrographis paniculata Ness and were infected withA. hydrophila (0.1 ml of 108 of A. hydrophila culture), P2 (Fishes were fed with feed addedwith 20 g of Psidium guajava and 20 g of Andrographis paniculata Ness and were infectedwith A. hydrophila (0.1 ml of 108 of A. hydrophila culture), P3 (Fishes were fed with feedadded with 30 g of Psidium guajava and 30 g of Andrographis paniculata Ness and wereinfected with A. hydrophila (0.1 ml of 108 of A. hydrophila culture). Fish organ (liver) wereprocessed for histological studied (formalin fixed, alcohol series, 6 sliced and HE Stained).Result indicate that the structure of liver of the treated fish showing abnormality symptoms,such as necrosis, fatted degeneration and hemorrhage. It can be concluded that feeding offish with 30 g of Psidium guajava and 30 g of Andrographis paniculata Ness is able to treatA. hydrophila infection on Mystus nemurus.Key word : Liver, Mystus nemurus, Psidium guajava, Andrographis paniculata Ness.
THE EFFECT OF THE ADDITION OF GINGER (Curcuma xanthorrhiza ROXB) TO FEED TO TOTAL ERYTHROCYTES, HEMATOCRIT, HEMOGLOBIN and GROWTH OF CATFISH (Mystus nemurus) Yulistia, Feri; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was done in March - April 2014 in Fish Disease Parasite and Laboratories and an Experiment of the Faculty of Fisheries and Marine Science University of Riau. The purpose of this research is to know the influence of the addition of curcuma on feed to the total of erythrocytes , hematocrit , hemoglobin and the growth of catfish. A research method used is the design of random complete with one factor which feed containing curcuma with four standard treatment consists of , P0: control without given ) curcuma solution , the P1 ( curcuma solution with a dose of 2 g/kilogram feed ) , the P2 ( curcuma solution with a dose of 4 g/kilogram feed ) , the P3 ( curcuma solution with a dose of 6 g/kilogram feed ). The results of research shows that the addition of a solution on feed curcuma doesn’t impact the condition of fish blood because of poor environmental conditions. It can increase the growth of absolute weight catfish .The total erythrocytes is ranged between 1,25-1,91 mm3/ million cells , hematocrit 13,8-18,5 % and the percentage of hemoglobin 3,9-4,2 g/dL.Keywords: River catfish (Mystus nemurus), ginger, blood catfish
SENSITIVITAS EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI Streptococcus agalactiae Muharram, Anzila Rizki W; Syawal, Henni; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTThe research was conducted from October to December 2014 in the Laboratory of Parasitic Diseases of Fish Faculty of Fisheries and Marine Sciences, and Laboratory of organic and natural material of Faculty of Mathematics and Natural Sciences, University of Riau, Pekanbaru. The purpose of this study to determine the concentration of noni fruit (Morinda citrifolia L.) that can inhibit the growth of Streptococcus agalactiae and safe dose to be given to the fish. The method that had been used was experimental method with concentration : P0: control, P1 (4%), P2 (5%), P3 (6%), P4 (7%), P5 (8%), P6 (9%), P7 (10%). The results show noni fruit solution to Streptococcus agalactiae sensitive average highest inhibition zone formed on treatment P7 (10%) of 9.83 mm and significantly different between treatments (P <0.05). MIC test of extract methanol Morinda citrifolia L. on treatment P2 (5%) of 78 x 108 cfu/mL. LD50 test solution for tilapia (Oreochromis niloticus) extract methanol Morinda citrifolia L. marinated for 24 hours after Reed and Muench analysis contained in the concentration of 1.42%.Key word: Morinda citrifolia L., Streptococcus agalactiae, Sensitivitas, MIC, LD50.