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DETECTION OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS FORMULA MILK IN BOGOR Nugroho, Widagdo Sri; Sudarwanto, Minarwati; Lukman, Denny Widaya; Usleber, Ewald
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn�s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John�s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57
DETEKSI PENYAKIT BOVINE VIRAL DIARRHEA PADA SAPI POTONG IMPOR MELALUI PELABUHAN TANJUNG PRIOK Primawidyawan, Aditya; Indrawati, Agustin; Lukman, Denny Widaya
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.354 KB) | DOI: 10.29244/avi.4.1.7-13

Abstract

Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi BVD terhadap 100 sampel serum darah sapi, dan ditemukan 63 positif terhadap adanya antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan ELISA Antigen BVD dan hasilnya seluruh sampel negatif terhadap Antigen BVD. Hasil positif uji ELISA terhadap antibodi BVD mengindikasikan bahwa sampel mengandung antibodi anti BVD akibat pernah terinfeksi oleh virus BVD secara sementara (transient) atau melalui vaksinasi. Berdasarkan dokumen health certificate dari negara asal tidak terdapat informasi yang jelas terhadap perlakuan vaksinasi BVD pada sapi potong impor. Oleh karena itu diperlukan pemeriksaan screening di negara Indonesia untuk mendeteksi dan melakukan usaha preventif mencegah penyebaran di feedlot. Faktor-Faktor yang mempengaruhi kejadian hasil ELISA antibodi positif BVD, terkait dengan penyebaran penyakit BVD selama dalam masa pemeliharaan dan penggemukkan adalah program biosekuriti pada peternakan dengan nilai (OR=3,316; CI=1,380-7,967), dan pengelolaan limbah kandang dalam peternakan dengan nilai (OR=2,667; CI=1,105-6,434). Hasil ini menunjukkan ada asosiasi antara kedua faktor yang ada pada peternakan dengan kejadian penyakit BVD.Kata kunci: BVD, ELISA antibodi dan antigen, faktor risiko. (Detection and Risk Factors Study of Bovine Viral Diarrhea in Cattle Imports at Tanjung Priok Port)This research was a serological study on bovine viral diarrhea (BVD) and also to detect the relevance of maintenance farm management as a risk factor on the spreads of BVD. The initial screening test was performed using antibody capture enzyme linked immunosorbent assay (ELISA) to BVD on 100 cattle blood serum samples. The screening test showed that of 63 samples were positive to BVD antibody and 37 samples were negative to BVD antibody. The next screening test was performed using antigen capture ELISA to BVD and all samples showed negative results on BVD antigen. The results of the ELISA test positive for antibodies to BVD indicates that the samples examined anti-BVD antibodies due to BVD virus had been infected by a temporary (transient) or vaccination. Based on the document health certificate from the country of origin there is no clear information on the treatment of BVD vaccination on imports of beef cattle. So, we need a screening examination in the country of Indonesia to detect and perform preventive measures to prevent the spread in feedlots. Relevant factors that affected the occurrence of positive result on BVD antibody detection was farm biosecurity programs with odds ratio (OR) value of 3.316 and confidential interval (CI) value of 1.380-7.967. Further relevant factor was caging waste management with OR value of 2.667 and CI value of 1.105-6.434. There were statistically significant differences (p<0.05) between farm biosecurity programs and caging waste management related to BVD disease incidence.Keywords: BVD, ELISA antibodies and antigen, risk factors
INDIRECT ENZYME LINKED IMMUNOSORBENT ASSAY SEBAGAI METODE UNTUK MELACAK BRUSELOSIS PADA SAPI PERAH (INDIRECT ENZYME IMMUNOSORBENT ASSAY (I-ELISA) AS METHOD FOR DETECT BRUCELLOSIS IN DAIRY COW) Ghurafa, Rinaldi; Lukman, Denny Widaya; Latif, Hadri
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.425 KB) | DOI: 10.19087/jveteriner.2019.20.1.30

Abstract

Brucellosis has become a zoonotic disease that received attention in efforts to prevent and eradicate strategic infectious animal diseases in Indonesia. Brucellosis can be detected early by the rose bengal test (RBT), followed by complement fixation test (CFT) and by enzyme linked immunosorbent assay (ELISA). The aims of this research was to study the indirect enzyme linked immunosorbent assay test (I-ELISA) as an alternative test for detecting brucellosis in dairy cattle. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated sensitivity and specificity, as well as analyzed by calculating the kappa value. The method was used by conducting tests of RBT, CFT, I-ELISA and commercial I-ELISA to test brucellosis. The test results were calculated for sensitivity and specificity, as well as analyzed by calculating the Kappa statistical value. The results of the sensitivity and specificity calculation showed that the indirect enzyme linked immunosorbent assay (I-ELISA) test developed a higher sensitivity (100%) compared to RBT test (93.75%) and commercial I-ELISA (93.75%). The developed I-ELISA specificity (74.68%) was still lower than RBT (89.87%), but higher than commercial I-ELISA (70.52%). The calculation of the statistical value of kappa RBT with CFT showed the kappa value 0.7120 which meaned it had a good agreement, commercial I-ELISA with CFT showed kappa value 0.6165 which meaned it had good suitability, whereas I-ELISA developed with CFT showed kappa value 0.4984 which meaned having a moderate agreement.In conclusion, the indirect enzyme linked immunosorbent assay (I-ELISA) which had been developed had low specificity, but the sensitivity was the highest compared to the commercial I-ELISA test and RBT, so this test was appropriate to be used as a screening test, especially in dairy cows movement into brucellosis-free areas or regions.
CROSS REACTION OF SERUM IN SALMONELLA ENTERITIDIS- VACCINATED CHICKEN TO SOME SALMONELLA ENTERICA SEROTYPES (REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) Arnafia, Wyanda; Ningrum, Siti Gusti; Puspita, Erfiandini Eka; Lukman, Denny Widaya; Pasaribu, Fachriyan Hasmi; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Salmonella spp. has been recognized as the major cause of food-borne illness in humans worldwidecausing remain relevant to public health. Poultry vaccination is one promising strategy to mitigateSalmonella infection in poultry and, in turn, in humans as well. The objective of this study was to assessthe potential of cross-reaction of serum in Salmonella enteritidis-vaccinated chicken to some serotype ofSalmonella enterica. Four female, Isa Brown layer chickens (20 weeks old), were vaccinated with S. enteritidisstrain Sm24/Rif12/Ssq (intra vena) to induced the production of specific antibodies in serum. Crossreactionof serum in S. enteritidis-vaccinated chicken were assess with agar gel immunodiffusion test(AGID) with S. enteritidis, S. pullorum, S. typhimurium, S. typhi, and Escherichia coli antigens. Serumcould react with S. enteritidis and all types of S enterica used in this study (S. pullorum, S. typhimurium,S. typhi), but could not react with E. coli. The potential of cross-reaction of serum in S. enteritidis-vaccinatedchicken to some serotypes of S. enterica may play a role in reducing the infection caused by that serotype.
KEBERADAAN BAKTERI LISTERIA MONOCYTOGENES PADA KEJU GOUDA PRODUKSI LOKAL DAN IMPOR (PRESENCE OF LISTERIA MONOCYTOGENES IN LOCAL AND IMPORTED GOUDA CHEESES) Pazra, Debby Fadhilah; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Listeria monocytogenes is included in the foodborne pathogen, which has been associated with severaloutbreaks of human listeriosis especially in high risk groups. Listeria monocytogenes could be found inGouda cheeses because of poor hygienic and sanitation practices. In addition, this bacteria could surviveduring the making of cheese and cheese ripening process. The purpose of this study was to identify thepresence of L. monocytogenes in local and imported Gouda cheeses and how the safety level of the Goudacheese against contamination of L. monocytogenes. This study used the conventional method in accordancewith the Bacteriological Analytical Manual, US Food and Drug Administration and Bergey?s Manual ofDeterminative Bacteriology to detect the presence of L. monocytogenes at 15 samples of local Gouda cheeseand 15 samples of imported Gouda cheese sold in supermarkets in Jakarta and Bogor. The results of thisstudy showed that was not found L. monocytogenes in local and imported Gouda cheese. It could be concludedthat is Gouda cheese relatively safe from L. monocytogenes and meets Indonesian National Standard.
SEROPREVALENSITRICHINELLOSISPADABABI DI KOTA KUPANG, PROVINSI NUSA TENGGARA TIMUR (SEROPREVALENCE OF TRICHINELLOSIS ON PIG AT KUPANG CITY, PROVINCE OF NUSA TENGGARA TIMUR) Angi, Andrijanto Hauferson; Satrija, Fadjar; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Sudarnika, Etih
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Trichinellosisis is a zoonotic disease caused by infection of Trichinella spp. and is found world-wide.The seroprevalence of trichinellosis in pig population has never been reported in East Nusa TenggaraProvince particularly Kupang City. The aim of this study was to determine the seroprevalence oftrichinellosis in pig at Kupang City, East Nusa Tenggara. A cross-sectional study was conducted byexamining 376 blood samples of pigs collected from slaughterhouses in Oeba, Kupang using simplerandom sampling method. Blood samples were tested by indirect enzyme linked immunosorbent assay(ELISA) using Screen®Trichinella indirect multi-species ELISA kit. Test results showed that 3 (0.8%)serum samples were positive of trichinella antibody. In conclusion ,some pigs in the city of Kupang hasbeen infected by Trichinella sp. and could be a threat for human health.
RESISTENSI ANTIBIOTIK PADA SALMONELLA ISOLAT SAPI BAKALAN ASAL AUSTRALIA YANG DIIMPOR MELALUI PELABUHAN TANJUNG PRIOK JAKARTA (ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTED FEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) Kurniawati, Anindya; Lukman, Denny Widaya; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Importation of feeder cattle from farms constantly using antibiotics in feed could result in the occurrenceof antibiotic resistant bacteria. A study was therefore conducted to investigate the possible use antibioticsand the antibiotic resistance of commensal Salmonella sppagainst several antibiotics. Salmonella spp.bacteria (n=50)were isolated from 100 samples of feeder cattlesfaeces. Total of 50 Salmonella spp. isolateswere subjected for Salmonella spp. examination and the isolated Salmonella spp. was tested for theantibiotic resistance using 10 antibiotics (ampicillin, cephalotin, erythromycin, tetracycline, streptomycin,chloramphenicol, trimethoprim, trimethoprim-sulfamethoxazole, nalidixic acid, and enrofloxacin) usingdisk diffusion method on Muller-Hinton agar following Clinical and Laboratory Standards Institute (CLSI)guidelines for interpretation. The isolated Salmonella showed resistance towards erythromycin 98%,ampicillin 34%, streptomycin 22%, nalidixic acid 8%, cephalotin 6%, tetracycline 4%, and chloramphenicol2%. There was no resistance against enrofloxacin, trimethoprim, and trimethoprim-sulfamethoxazole. Salmonella spp. bacteria derived from imported feeder cattle which have been resistant to antibiotics arepotential for spreading the antibiotic resistant bacteria to other susceptible animals. Such bacteria canalsotransfer the antibiotic resistant gene to other bacteria in Indonesia which ould be a potential threatfor public and animal healths.
KEBERADAAN KOLIFORM PADA DAGING AYAM SUWIR BUBUR AYAM YANG DIJUAL DRAMAGA BOGOR Zelpina, Engki; Purnawarman, Trioso; Lukman, Denny Widaya
Jurnal Teknologi Pangan dan Gizi Vol 19, No 1 (2020)
Publisher : Widya Mandala Catholic University Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.4 KB) | DOI: 10.33508/jtpg.v19i1.2447

Abstract

Penelitian ini bertujuan untuk mengetahui cemaran bakteri koliform pada daging ayam suwir bubur ayam yang dijual Dramaga Bogor. Sampel daging ayam suwir bubur ayam yang digunakan berasal dari pedagang bubur ayam. Sejumlah 15 sampel daging ayam suwir bubur ayam di isolasi terhadap bakteri koliform. Metode yang digunakan adalah cara tuang (pour plate method). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa jumlah cemaran koliform pada daging ayam suwir bubur ayam yang dijual di Dramaga Bogor adalah 4,25 x 105 ± 2,74 x 105. Cfu/g. Jumlah koliform pada daging ayam suwir bubur ayam melebihi SNI sehingga dapat menimbulkan berbagai gangguan kesehatan masyarakat.
RESISTENSI SALMONELLA SPP. TERHADAP BEBERAPA ANTIBIOTIK PADA DAGING ITIK DI KABUPATEN BOGOR YANG DAPAT MEMENGARUHI KESEHATAN KONSUMEN (RESISTANCE OF SALMONELLA SPP. TO SEVERAL ANTIBIOTICS FROM DUCK MEAT IN BOGOR DISTRICT THAT COULD INFLUENCE CONSUMER HEALTH) Loisa, Loisa; Lukman, Denny Widaya; Latif, Hadri
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.189 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5040

Abstract

This study aimed to determine the presence of Salmonella spp. in duck meat and to identify the resistance of Salmonella spp. against several antibiotics in duck meat associated with food safety. Total of 52 meat samples of ducks was collected from 5 subdistricts in Bogor District, i.e., Cariu-Jonggol, Gunung Sindur, Klapanunggal, Parung Panjang, and Ciomas based on the assumption of 95% confidence level, 50% predicted prevalence, and 10% standard error. The results showed that three samples (5.8%) were positive Salmonella spp. The majority of Salmonella spp. isolated from duck meat showed resistance against erythromycin (66.7%), streptomycin (33.3%), and chloramphenicol (33.3%). Nevertheless, Salmonella spp. was still sensitive againts enrofloxacin, trimethoprim sulfamethoxazole, cephalothin, ampicillin, nalidixid acid, tetracycline, and gentamicin.
IDENTIFIKASI PENAMBAHAN AIR PADA DAGING SAPI DENGAN METODE FILTER PAPER PRESS DAN KONDUKTIVITAS LISTRIK Adinata, Nararya; Lukman, Denny Widaya; Sudarnika, Etih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.723 KB) | DOI: 10.22146/jsv.33447

Abstract

Beef meat has a complete nutritional content, the high prices in the market causing some unscrupulous traders to cheat by adding water to meat to get more weight. This beef meat has a bad quality, so needs the right method to identify the quality of beef meat in the field.  This study was designed to identify the addition of water to beef meat by measuring the water holding capacity (filter paper press method) and electrical conductivity (EC meter). The experimental used one hundred bovine longisimus dorsi and the samples were divided into five groups. One group as a control and four other groups were treatment group. The treatment groups were injected by water with a volume of 1%, 2%, 3%, and 4% of the sample weight and all groups were repeated for twenty times. The results were analyzed by ANOVA and Duncan Multiple Range test. The data showed that the addition of water by injection in beef meat of treatment group 2%, 3%, and 4% had a significant influence on water holding capacity and electrical conductivity (p<0.05). Pearson correlation results showed a strong to very strong correlation with value in control group (r=0.704; p<0.01), treatment group 1% (r=0.628; p<0.01), 2% (r=0.700; p<0.01), 3% (r=0.642; p<0.01) and 4% (r=0.760; p<0.01). Based on the result of the study, it is known that a water increase in beef at least 2% of the weight significantly affected water holding capacity and it can be identified by using electrical conductivity meter.