I Gusti Ngurah Mahardika
Laboratorium Biomedik Fakultas Kedokteran Hewan,Universitas Udayana

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REVALENSI PORCINE CIRCO VIRUS SECARA SEROLOGIS PADA PETERNAKAN BABI DI BALI Suartha, I Nyoman; Anthara, I Made Suma; Wirata, Wayan; Krisna Dewi, Ni Made Ritha; Narendra, I Gusti Ngurah; Mahardika, I Gusti Ngurah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.488 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2790

Abstract

Penelitian ini bertujuan mengetahui seroepidemiologi infeksi porcine circo virus (PCV-2) dua pada peternakan babi di Bali. Pada penelitian ini sampel yang dianalisis sebanyak 295 sampel. Sampel berasal dari peternakan babi rakyat sebanyak 98 dan dari peternakan babi intensif sebanyak 197. Sampel berasal dari delapan kabupaten dari sembilan kabupaten yang ada di Bali. Deteksi antibodi dilaksanakan dengan uji enzyme-linked immunosorbent assay (ELISA) dan deteksi virus dilakukan dengan polymerase chain reaction (PCR). Hasil penelitian menunjukkan bahwa seroprevalensi antibodi anti-PCV-2 adalah 84,1%, dengan sebaran di peternakan rakyat dan peternakan intensif masingmasing sebesar 70,4 dan 91,2%. Semua peternakan babi intensif menunjukkan antibodi positif. Prevalensi virus PCV-2 di seluruh Bali sebesar 1,7% dengan sebaran pada peternakan rakyat peternakan intensif masing-masing sebesar 3,1 dan 1,0%. Dari hasil penelitian disimpulkan bahwa infeksi PCV-2 pada peternakan babi di Bali bersifat endemis.
STRUKTUR GENETIK DAN FILOGENI YELLOWFIN TUNA (THUNNUS ALBACARES) BERDASARKAN SEKUEN DNA MITKONDRIA CONTROL REGION SITOKROM OKSIDASE I PADA DIVERSITAS ZONE BIOGEOGRAFI Wijana, I Made Sara; Mahardika, I Gusti Ngurah
Bumi Lestari Journal of Environment Vol 10 No 2 (2010)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.163 KB)

Abstract

Genetic structure and phylogeni of 37 sequences control region DNA mitochondrial cytocrome oxidase I of yellowfin tuna (Thunnus albacores) have been downloaded from GeneBank and analised using Maximum Likehood (ML), Pairwise Genetic Distance and Bootstrapping Phylogeni Model of Kimura 2 Parameter. The result shows that the corrected value of the data was 5,5% and the mean of genetic distances was 3,7 %, where the shortest distance was 0.0% and the longest was 5%. The genetic distances with the out group (Thunnus obesus) ranged between 7,8 % â?? 9,8 % and with the Thunnus thynus ranged between 10,4% â?? 12,5%. The value of bootstrap phylogeny of 37 sequences of yellowfin tuna was less than 50%. All those results shows that there was no significant genetic differences of 34 samples sourced from Philipines and 3 from Spain based on sequence region DNA mitochondrial cytocrome oxidase I.