Articles

INVESTIGASI PENYAKIT PADA PEMBESARAN LOBSTER PASIR PANULIRUS HOMARUS DI KARAMBA JARING APUNG (LOMBOK, PEGAMETAN DAN PANGANDARAN) sudewi, .; Widiastuti, Zeny; Slamet, Bejo; Mahardika, Ketut
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 1 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.133 KB) | DOI: 10.29244/jitkt.v10i1.18976

Abstract

Lobster pasir, Panulirus homarus adalah salah satu komoditas perikanan yang memiliki nilai ekonomis tinggi, baik di pasar lokal maupun internasional. Akan tetapi, dalam usaha pembesaran lobster terdapat hambatan yaitu tingginya mortalitas yang disebabkan oleh infeksi penyakit. Oleh karena itu, dilakukan penelitian ini dengan tujuan untuk melakukan investigasi penyakit pada pembesaran lobster pasir P. homarus di tiga lokasi karamba jaring apung (KJA). Metode penelitian meliputi pengambilan sampel lobster, pengamatan parasit, isolasi bakteri dan jamur, serta deteksi Milky Hemolymph Disease of Spiny Lobster (MHD-SL) melalui analisis Polymerase Chain Reaction (PCR). Lokasi pengambilan sampel meliputi Lombok (NTB), Pangandaran (Jabar) dan teluk Pegametan (Bali) dengan sampel lobster masing-masing sebanyak 5 ekor. Tiga ekor lobster dari Pangandaran terinfeksi parasit Octolasmis sp. yang menginfeksi terutama pada lamela insang. Satu sampel lobster dari Lombok terinfeksi jamur Fusarium sp. yang merupakan penyebab penyakit black gill disease dengan gejala insang yang menghitam. Dua lobster dari Lombok dan 1 lobster dari Pegametan terinfeksi oleh MHD-SL yang ditandai dengan hemolimfa berwarna putih susu.
ANALISIS KOMPARATIF KINERJA PUSKESMAS DENPASAR SELATAN DAN DENPASAR TIMUR DENGAN MENGGUNAKAN METODE BALACED SCORECARD Mahardika, Ketut; Supadmi, Ni Luh
E-Jurnal Akuntansi Vol 8 No 1 (2014)
Publisher : Accounting Department, Economic and Business Faculty of Universitas Udayana in collaboration with the Association of Accounting Department of Indonesia, Bali Region

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Abstract

Health centers are the top of public health services in Indonesia. With the health centers, each people can get health care easily and affordable. In the past, health center performance is more often focused on customer satisfaction or patient. This study is a descriptive comparative study aimed to determine and compare the performance of health centers in south and east Denpasar using the balanced scorecard method. The health centers in south and east Denpasar chosen as the study site because the health centers in the two districts are already implementing a quality management system in accordance with Health Department standards and there is a health center that operates 24 hours in each. The study used primary data obtained from the answers given by the respondents and using secondary data from health centers financial data. Variables examined include the performance of the financial perspective, customer perspective, internal business processes, and learning and growth perspective.
IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER PANULIRUS HOMARUS Sudewi, Sudewi; Widiastuti, Zeny; Mastuti, Indah; Mahardika, Ketut
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3578

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.   
PERANG ANTARA KERAJAAN BULELENG DENGAN BELANDA PADA TAHUN 1846-1849 Mahardika, Ketut; Amsia, Tontowi; Wakidi, Wakidi
PESAGI (Jurnal Pendidikan dan Penelitian Sejarah) Vol 2, No 2 (2014): PESAGI (Jurnal Pendidikan dan Penelitian Sejarah)
Publisher : FKIP UNIVERSITAS LAMPUNG

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Abstract

The problems of the study are: how the occurrence of the Buleleng Bali war with the Netherlands in 1846-1849. The purpose of the study was to find out the occurrence of war between the kingdoms of Buleleng with the Netherlands in 1846-1849. The method uses in the study is the historical method. Data collection techniques are literature technique and documentation technique, data analysis that used is qualitative. Before the war between Buleleng and the Netherland held mediation but not found a solution. In June 28, 1846 the first attack was done but there was no counter for buleleng because the far less modern weapons of the Buleleng kingdom. The second attack cause of the expropriation of the Dutch ship in the Lirang beach,  Buleleng is not received because the Dutch established Fort. Rumusan masalah dalam penelitian adalah: bagaimanakah terjadinya perang kerajaan Buleleng Bali dengan Belanda pada tahun 1846 – 1849. Tujuan penelitian adalah mengetahui terjadinya perang antara kerajaan Buleleng dengan Belanda pada tahun 1846 – 1849. Metode yang digunakan dalam penelitian adalah metode historis. Teknik pengumpulan data menggunakan teknik kepustakaan dan teknik dokumentasi, analisis data yang digunakan adalah kualitatif. Sebelum terjadinya perang antara Buleleng dengan Belanda diadakan mediasi tetapi tidak ditemukan penyelesaian. 28 Juni 1846 penyerangan pertama dilakukan tetapi tidak adanya perlawanan dari Buleleng dikarenakan senjata yang kalah jauh modern. Penyerangan kedua dikarenakan terjadinya perampasan kapal Belanda di pantai Lirang, Buleleng tidak terima karena Belanda mendirikan benteng. Kata kunci : belanda, kerajaan buleleng, perang
APLIKASI SISTEM RESIRKULASI PADA PENDEDERAN IKAN KAKAP PUTIH, LATES CALCARIFER KEPADATAN TINGGI Permana, Gusti Ngurah; Pujiastuti, Zeny; Fakhrudin, Fakhrudin; Muzaki, Ahmad; Mahardika, Ketut; Adiyana, Kukuh
Jurnal Riset Akuakultur Vol 14, No 3 (2019): (September, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.14.3.2019.173-182

Abstract

 Teknologi resirkulasi (Recirculating aquaculture system [RAS]) dikembangkan untuk meningkatkan produktivitas benih ikan kakap putih. Tujuan dari penelitian ini untuk mengetahui pengaruh perbedaan kepadatan pada pendederan benih ikan kakap dengan sistem RAS. Penelitian ini menggunakan sistem RAS dengan 12 bak dengan volume 1,0 m3 yang terbagi dalam dua modul dengan masing-masing modul terdiri atas enam bak. Untuk perbandingan digunakan sistem sirkulasi yang mengadopsi teknologi yang ada di masyarakat menggunakan tiga bak beton dengan volume 1 m3. Benih ikan kakap putih yang dipergunakan memiliki panjang rata-rata: 2,87 cm ± 0,18 cm dan bobot rata-rata: 0,39 ± 0,07 g. Perlakuan menggunakan perbedaan kepadatan yaitu (A) 3.000 ekor/m3 (1,17 kg/m3); (B) 4.500 ekor/m3 (1,75 kg/m3); dan (C) sirkulasi 1.500 ekor/m3 (0,62 kg/m3). Parameter yang diamati meliputi: pertumbuhan, sintasan, kualitas air, dan pada akhir penelitian, sampel darah diambil untuk menentukan kesehatan ikan yang berhubungan dengan parameter haematokrit darah. Analisis data secara deskriptif dan uji t test. Hasil penelitian menunjukkan bahwa perbedaan padat penebaran pada sistem RAS dan sirkulasi secara signifikan tidak menunjukkan perbedaan (P&gt;0,05) pertumbuhan panjang dan bobot benih. Sintasan tertinggi diperoleh pada kelompok ikan kontrol, diikuti dengan kelompok ikan dengan kepadatan 3.000 ekor/m3 dan terendah pada kelompok ikan dengan kepadatan 4.,500/m3 ekor. Nilai hematokrit dalam darah lebih tinggi (P&lt;0,05) ditunjukkan dari kelompok ikan dengan kepadatan yang lebih rendah. Hasil tersebut menunjukkan bahwa aplikasi sistem resirkulasi pada pendederan ikan kakap putih dapat dilakukan dengan penerapan kepadatan 3.000 ekor/m3 (31,04 kg/m3).Recirculating aquaculture system (RAS) has been developed to increase the productivity of barramundi nursery. This study used 12 tanks with a volume of 1.0 m3 each. The tanks were grouped into two modules, each module consisted of six tanks. As a comparison, a circulation system adopted by the local community was used which consisted of three concrete tanks with a volume of 1 m3. Barramundi juveniles with an average length: 2.87 cm ± 0.18 cm and an average weight of 0.39 ± 0.07 g) were used in the experiment. The treatments were differences in stocking densities: 3,000 fish/m3 (1.17 kg/m3); 4,500 fish/m3 (1.75 kg/m3); and control 1,500 fish/m3 (0.62 kg/m3). Data collected included growth of survival and water quality variables (temperature, salinity, DO, pH, nitrite, NH3, total bacteria/vibrio) and blood hematocrit. The results of this study showed that fish densities (4,500; 3,000; and 1,500 fish/m3) did not affect fish growth. However, the survival rate was significantly different (P&lt;0.05) among the treatments. The values of hematocrit were significantly (P&lt;0.05) higher at the density of 46.56 kg/m3. These results suggest that the RAS application can sustain a nursery density of Barramundi up to 3,000 ind./m3 (31.04 kg/m3).
APLIKASI ASTAXANTHIN DARI HAEMATOCOCCUS PADA BENIH KERAPU SUNU (PLECTROPOMUS LEOPARDUS) TERHADAP TOTAL KAROTENOID DAN PROFIL DARAH Kusumawati, Daniar; Mahardika, Ketut; Setiawati, Ketut Maha
Media Akuakultur Vol 14, No 2 (2019): (Desember, 2019)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.14.2.2019.113-122

Abstract

Haematococcus merupakan alga yang kaya karotenoid dari jenis astaxanthin yang tidak hanya berpotensi sebagai sumber pigmen merah tetapi juga sebagai antioksidan. Aplikasi haematococcus telah dilakukan pada larva kerapu sunu dan menunjukkan adanya perbaikan peformansi warna merah yang cukup signifikan. Tujuan dari penelitian ini adalah mengetahui pengaruh haematococcus sebagai sumber astaxanthin terhadap pertumbuhan, total karotenoid, dan profil darah (hematokrit dan haemoglobin) pada juvenil ikan kerapu sunu. Benih kerapu sunu ukuran panjang rata-rata 14,07 ± 0,07 cm dan bobot rata-rata 45,92 ± 6,35 g dipelihara dalam jarring berukuran 0,5 m x 0,5 m x 1 m yang diletakkan dalam bak beton ukuran 3 m x 1,2 m x 1,2 m. Kepadatan ikan tiap jaring adalah lima ekor. Perlakuan yang diujicobakan adalah penambahan haematococcus ke dalam pakan buatan dengan dosis dan kompisisi sebagai berikut: A) 1% dari berat pakan, B) 1% dari berat pakan + 10% minyak ikan, dan C) 0% (kontrol). Hasil penelitian menunjukkan bahwa penambahan haematococcus ke dalam pakan tidak memberikan perbedaan nyata terhadap pertumbuhan mutlak panjang dan bobot, serta laju pertambahan panjang dan bobot (P value &gt; 0,05). Penambahan haematococcus memberikan perbedaan nyata (P value &lt; 0,05) terhadap konversi pakan di mana perlakuan A (1,99 ± 0,09); B (2,12 ± 0,14); dan C (2,28 ± 0,09). Penambahan haematococcus memberikan peningkatan terhadap akumulasi kandungan total karoten, hematocrit, dan haemoglobin darah.Haematococcus, an alga rich in carotenoids of the astaxanthin type, not only has the potential as a source of red pigment but also as an antioxidant. The purpose of this study was to determine the effects of hematococcus as astaxanthin source on the growth, total carotenoids, and blood profile (hematocrit and hemoglobin) of coral trout grouper juvenile. Coral trout grouper seed with an average length of 14.07± 0.07 cm and an average weight of 45.92 ± 6.35 g were maintained in a net cage measuring 0.5 m x 0.5 m x 1 m placed in a 3 m x 1.2 m x 1.2 m concrete tank. Fish density per net was five fish. The treatment tested was the addition of haematococcus into the artificial feed with the following dosages and compositions: A) 1% of the weight feed, B) 1% of the weight feed + 10% fish oil, C) 0% (control). The results showed that the addition of haematococcus to the feed did not give a significant difference to the absolute growth of length and weight and also specific growth and length rate (P-value &gt; 0.05). The addition of haematococcus gave a significant difference (P-value &lt; 0.05) to feed conversion ratio between treatment A, B and C with the values of 1.99 ± 0.09, 2.12 ± 0.14, and 2.28 ± 0.09, respectively. The addition of haematococcus also increased the levels of total carotene content, hematocrit, and hemoglobin. This study suggests that the application of haematococcus could significantly improve the red color performance of trout grouper larvae.
ELECTRON MICROSCOPIC STUDY ON ENLARGED CELLS OF RED SEA BREAM, Pagrus major INFECTED BY THE RED SEA BREAM IRIDOVIRUS (RSIV, GENUS Megalocytivirus, FAMILY Iridoviridae) Mahardika, Ketut
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1450.737 KB) | DOI: 10.15578/iaj.4.1.2009.53-63

Abstract

Most histopathologycal studies of the red sea bream iridovirus (RSIV) disease in red sea bream have been performed by studying enlarged cells as well as necrotized cells in the spleen and other organs. These enlarged cells have been named as inclusion body bearing cells (IBCs). However, few information is available about detail of ultrastructural features of IBCs produced in the target organs of RSIV-infected fish. In the present study, details of ultrastructural features of IBCs that were produced in the spleen tissue of naturally RSIV-infected red sea bream were investigated under electron microscope. Under electron microscope, RSIV-infected red sea bream had the presence of two types of IBCs: typical IBCs allowing virus assembly within viral assembly site (VAS), and atypical IBCs which degenerate organelles without virus assembly. Other infected-cells were observed as necrotized cells forming intracytoplasmic VAS with large numbers of virions, but without the formation of the distinct inclusion body. Morphogenesis steps on RSIV-infected red sea bream were observed as filamentous-filed virions, partially-filled virions and complete virions with 145-150 nm in size. These findings confirmed that RSIV-infected red sea bream were characterized by formation of typical and atypical IBCs as well as necrotized cells.
HISTOLOGICAL STUDY ON INTERNAL ORGANS DEVELOPMENT OF MANGROVE SNAPPER, Lutjanus argentimaculatus LARVAE Mahardika, Ketut; Mastuti, Indah; Melianawati, Regina; Zafran, Zafran
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.294 KB) | DOI: 10.15578/iaj.7.2.2012.133-138

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Mangrove snapper, Lutjanus argentimaculatus is one of highly economic value of marine fish commodity. Culture of this species has been developed a few years ago. Basic data on biological aspect is still limited whereas those kinds of information are needed. Histological study to determine development of internal organs of the larvae of mangrove snapper was conducted. Amount of 15-20 larvae each from 1 day after DAH hatching (1 DAH) up to 30 days after hatching (30 DAH) were collected, fixed in Bouin’s solution for 4-6 hours and then transferred into 70% alcohol. The samples were then embedded in paraffin wax and the sections were stained with haematoxylineosin (H&amp;E). Results showed that the eye retina of the mangrove snapper larvae well developed on 3 DAH, whereas their digestive tract was on 5 DAH. Under this condition, the larvae started feeding as showed by present of natural food tissues in the intestine. Development of digestive tract and other internal organs reached their complete form like in adult fish after 20 DAH.
DISTRIBUTION ANALYSIS OF ENLARGED CELLS DERIVED FROM GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTED HUMPBACK GROUPER Cromileptes altivelis Mastuti, Indah; Mahardika, Ketut
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1548.747 KB) | DOI: 10.15578/iaj.7.1.2012.55-60

Abstract

Characteristic of Megalocytivirus infection has been known to produce formation of inclusion body bearing cells (IBCs) on internals organs of fish predominantly on spleen and kidney. Megalocytivirus that infected grouper is known as Grouper Sleepy Disease Iridovirus (GSDIV). This study was conducted to answer the effect of entry sites of GSDIV on distribution of enlarged cells formed on the internal organs of humpback grouper Cromileptes altivelis. Enlarged cells were observed histologically under the light microscope on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, intestine, muscle and brain. Entry sites were designated to intramuscularly injection, intraperitoneally injection, dipped gill and inoculum added feed. Enlarged cells were formed on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, muscle, except on intestine and brain. All the entry sites resulted in formation of enlarged cells on spleen, head kidney, trunk kidney, liver, heart. Spleen and head kidney were the most frequent observed organ. These results suggested that distribution of enlarged cells were not affected by the entry site of GSDIV.
EFFECTIVITY OF INACTIVE GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) VACCINE IN GROUPER FISH (Cromileptes altivelis and Epinephelus fuscoguttatus) AGAINST GSDIV INFECTION Mahardika, Ketut; Mastuti, Indah; Haryanti, Haryanti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1197.996 KB) | DOI: 10.15578/iaj.8.2.2013.143-151

Abstract

Grouper sleepy disease iridovirus (GSDIV) has been known as viral agent causing mass mortality and significant economic losses in Indonesian aquaculture industry. The aim of this research was to know the effectivity of formalin-inactivated GSDIV vaccine to prevent GSDIV infection in humpback grouper (Cromileptes altivelis) and tiger grouper (Epinephelus fuscoguttatus). The vaccine was derived from GF cells infected-GSDIV which activated using formalin. Used vaccine was contained titer virus of 102,80-105,80 TCID50/ mL. Result of vaccination test indicated that a vaccinated grouper fish with formalininactive GSDIV vaccine should decreased mortality rate of 5%–85.4% in humpback grouper and tiger grouper that infected with GSDIV. Histopathologically, infected fish from vaccinated and control groups showed formation of enlarged cells as well as necrotized cells especially in spleen and kidney tissues. On the other hand, surviving fish from both vaccinated and control groups did not contained formation of enlarged cells in spleen, kidney, liver, and stomach.