DESAK MADE MALINI
Department of Biology, Faculty of Mathematics and Natural Science, Padjadjaran University

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MALFORMASI RANGKA FETUS TIKUS WISTAR AKIBAT EFEK TERATOGENIK INSEKTISIDA ENDOSULFAN (SKELETAL MALFORMATION OF WISTAR RAT FETUSES DUE TO THE TERATOGENIC EFFECT OF ENDOSULFAN INSECTICIDE) Malini, Desak Made; Madihah, Madihah; Shalihah, Amalia
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.3.318

Abstract

Endosulfan an organochlorine insecticide that is commonly used even though it has been banned due to its toxic and teratogenic effect. This study aims to determine the effect of orally endosulfan exposure to pregnant rat (Rattus norvegicus) at day 6-15th of gestation period to the foetus skeletal malformation. A Completely Randomized Design (CRD) by endosulfan dosages: 0 (control); 0.083; 0.190; 0.440; 1.000 mg/kg BW/day with 5 replicates was applied. At 20th of the gestational period, the rats were sacrificed, their reproductive organs and the fetal skeletal malformation were observed using Alizarin red S method. The results of one-way ANOVA test showed that endosulfan exposure did not significantly affect the pregnancy outcomes ie. female rats weight gain, the numbers of implanted foetus, foetal body weight and length, respectively. Malformation of foetus implantation, foetus size and foetus skeletal occured in the endosulfan exposure groups. Fisher?s exact test results showed a significant difference between the control group and the endosulfan exposure group on the number of foetuses that have abnormalities in the number of skeletons of the sternum, fore and hind paws. The exposure of endosulfan at 0.083-1.000 mg / kg BW / day in pregnant rats caused skeletal malformations of the foetus ie. decreased in the number on sternum, fore- and hind paw bones. ABSTRAK Endosulfan merupakan insektisida golongan organoklorin yang masih digunakan hingga kini meski telah dilarang karena bersifat toksik dan teratogenik. Penelitian ini bertujuan untuk mengetahui efek pemajanan endosulfan secara oral terhadap malformasi rangka fetus tikus (Rattus norvegicus Berkenhout, 1769) selama umur kebuntingan 6-15 hari. Penelitian dilakukan secara eksperimental di laboratorium menggunakan Rancangan Acak Lengkap (RAL) dengan pemajanan endosulfan dosis: 0 (kontrol); 0,083; 0,19; 0,44; dan 1 mg/kg BB/hari dan masing-masing diulang sebanyak lima kali. Induk betina dengan umur kebuntingan 20 hari, dikorbankan nyawanya lalu dibedah dan diamati tampilan reproduksi induk serta malformasi rangka pada fetus dengan metode pewarnaan Alizarin red S. Hasil uji sidik ragam satu arah menunjukkan pemajanan endosulfan tidak berpengaruh secara signifikan terhadap rataan pertambahan bobot badan induk, jumlah fetus terimplantasi, bobot badan dan panjang fetus. Malformasi implantasi fetus, ukuran fetus dan rangka fetus terjadi pada kelompok pemajanan endosulfan. Hasil uji eksak Fisher menunjukkan adanya perbedaan yang signifikan antara kelompok kontrol dengan kelompok pemajanan endosulfan terhadap jumlah fetus yang mengalami kelainan jumlah rangka penyusun sternum, cakar depan dan cakar belakang. Simpulan dari penelitian ini adalah pemajanan endosulfan dosis 0,083- 1,000 mg/kg BB/hari pada tikus bunting menimbulkan malformasi rangka pada fetus yaitu berkurangnya jumlah tulang penyusun sternum, cakar depan, dan cakar belakang.
DISTRIBUTION OF FISH IN RIVER IS CONTROLLED BY PHYSICO-CHEMICAL PROPERTIES OF THE WATER WHICH IS AFFECTED BY LAND-USE COMPLEXITY AND INTENSITY OF HUMAN INTERVENTION. A STUDY ON FISH DISTRIBUTION WAS CARRIED OUT IN THE UPPER CITARUM RIVER TO MAP THE EFFECTS OF PHYSIO-CHEMICAL PROPERTIES ON HABITAT USE. A SURVEY WAS CONDUCTED TO COLLECT FISH AND TO MEASURE THE WATER QUALITY BOTH ON DRY AND RAINY SEASON. THE RESULT SHOWED THAT DISTRIBUTION OF THE FISH, IN GENERAL, REPRESENTED THEIR ADAPTIVE RESPONS SUNARDI, .; KANIAWATI, KEUKEU; HUSODO, TEGUH; MALINI, DESAK MADE; ASTARI, ANNISA JOVIANI
HAYATI Journal of Biosciences Vol. 19 No. 4 (2012): December 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.4.191

Abstract

Distribution of fish in river is controlled by physico-chemical properties of the water which is affected by land-use complexity and intensity of human intervention. A study on fish distribution was carried out in the upper Citarum River to map the effects of physio-chemical properties on habitat use. A survey was conducted to collect fish and to measure the water quality both on dry and rainy season. The result showed that distribution of the fish, in general, represented their adaptive response to physico-chemical properties. The river environment could be grouped into two categories: (i) clean and relatively unpolluted sites, which associated with high DO and water current, and (ii) polluted sites characterized by low DO, high COD, BOD, water temperature, NO3, PO4, H2S, NH3, and surfactant. Fish inhabiting the first sites were Xiphophorus helleri, Punctius binotatus, Xiphophorus maculatus, and Oreochromis mossambicus. Meanwhile, the latter sites were inhabited by Liposarcus pardalis, Trichogaster trichopterus, and Poecilia reticulata. Knowledge about fish distribution in association with the pysico-chemical properties of water is crucial especially for the river management.
KONSUMSI OKSIGEN IKAN PELAGIS DI MUARA SEGARA ANAK, TAMAN NASIONAL ALAS PURWO Malini, Desak Made; Muliani, Reni
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2489

Abstract

Taman Nasional Alas Purwo (TNAP) merupakan salah satu protected area yang ditetapkan sebagai kawasan untuk melestarikan keanekaragaman hayati dan ekosistem. Untuk mencapai tujuan tersebut maka perlu diadakan monitoring tentang kondisi dari kawasan tersebut, yaitu dengan cara mengamati biota perairan seperti ikan pelagis yang habitatnyadi sungai Segara Anak. Kelangsungan hidup ikan ditentukan oleh kondisi lingkungan dan kemampuan ikan untuk menggunakan oksigen dari lingkungannya. Tujuan dari penelitian ini adalah untuk mengetahui jumlah konsumsi oksigen ikan pelagis yang hidup di muara Segara Anak TNAP. Metode yang digunakan adalah observasi. Parameter yang diukur adalah konsumsi O2, kondisi fisik dan kimia dari habitat ikan. Hasil penelitian menunjukkan terdapat 4 jenis ikan pelagis dengan masing-masing konsumsi O2 sebagai berikut Scatophagus argus (0,15 mg/l /h), Toxotes jaculatrix (0,052 mg/l/h), Nectamia fusca (0,052mg/l/h) dan Ambassis macracanthus (0,095 mg/l/h). Tingkat konsumsi oksigen tertinggi ditunjukkan oleh S. argus. Hasil pengukuran parameter fisik dan kimia air adalah sebagai berikut: suhu 28oC, salinitas 33,3%; pH 8; dan oksigen terlarut 5 mg/l, sedangkan suhu udara 30,67oC. Tingkat konsumsi oksigen ikan tergantung pada jenis spesies, ukuran, dan kondisi fisik air.
PENGARUH PENAMBAHAN TEPUNG Spirulina fusiformis PADA PAKAN TERHADAP TINGKAT KECERAHAN WARNA IKAN KOI (Cyprinus carpio L.) Malini, Desak Made; K. P, Tri Dewi; Agustin, Resty
Pro-Life Vol 5 No 2 (2018): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v5i2.712

Abstract

Koi carp (C. carpio L.) is one of ornamental fish which has interesting color. The color of C. carpio L influenced its market price. Quality color of C. carpio L must be increased and it could be increased the market price of C. carpio L. Research aims to determine the effect of  Spirulina fusiformis powder addition in diet to enhance color brightness of C. carpio L. The research method used was experimental with one factorial Randomized Complete Design with four treatments and six replicatoins. The concentration of S. fusiformis powder given was 1%, 3%, and 5%. Treatments were given for 28 days and next 28 days without S.fusiformis powder addition. The parameter observed was the color brightness of C. carpio L. The observations used Toca Color Finder (TCF). Data was analyzed statistically by ANOVA. The results showed that addition of S. fusiformis powder in diet influenced brightness color of C. carpio L. Feeding with 1% of  S. fusiformis in diet resulted in a brighter color compared to other treatments and control on enhancement color brightness of C. carpio L. Results on TCF showed that level of color brightness of C. carpio L was increased. It can be concluded that adding of S. fusiformis powder in diet can increase brightness color of C. carpio L.   Key words: Ciprinus  carpio,  Color brightness, Spirulina fusiformis.
Alteration of Gills and Liver Histological Structure of Cyprinus carpio Exposed to Leachate Pribadi, Tri Dewi Kusumaningrum; Syahidah, Dzikrina; Harjanti, Sairandri Dyah; Malini, Desak Made
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8972

Abstract

One of the main problems in the waste management in Indonesia is the treatment of leachate, which mostly dumped to the river This research is aimed to obtain information of histological alteration in gills and liver of C. carpio L. exposed to leachate. Measurements on the water quality parameters comprised water temperature, pH, and dissolved oxygen (DO). This research was conducted by exposing leachate to C. carpio for 96 hours. The concentration of leachate were 0 ppm, 80 ppm, and 100 ppm. Histological preparation were made on the gills and liver using 10% fixative Neutral Buffered Formalin and Ehrlich Hematoxylin-Eosin staining with qualitative observation descriptive analyses for discussion. The result showed that increasing water temperature is directly proportional to the leachate concentration in the aquaria, while the value of pH and DO inversely proportional to the leachate concentration. Damages on the gills with 80 ppm leachate concentrasion were identified as follows: fusion of secondary gill filaments and hyperplasia of epithelial cell, along with karyorrhexis and hydropic degeneration on the liver. Damages on the gills of fishes exposed to leachate with 100 ppm concentrasion were identified as follows: fusion of secondary gill filaments, hyperplasia of epithelial cell, congestion, and edema along with karyorrhexis, hydropic degeneration and melanomacrophage centre (MMC) found on the liver. The results of this study can be used as an overview of the impact of an environmental pollution by leachate as indicated from histological damage to the gills and liver of C. carpio, thus contribute significan information to aquaculture sector and endorse better waste management
Ethnobotanical Study of Medicinal Plants in Karangwangi, District of Cianjur, West Java Malini, Desak Made; Madihah, Madihah; Kusmoro, Joko; Kamilawati, Fitri; Iskandar, Johan
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.5756

Abstract

The knowledge and usage of plant as medicinal remedy by current generation are not as extensive as previous; therefore, many rural communities with restricted modern medical access still rely on traditional medicine. This paper provides significant ethnobotanical information on medicinal plants in Karangwangi Village of Cianjur District, West Java Indonesia. This study aimed to identify plants collected for medical purposes by the local people as well as to document the local names, uses, preparation, and location of these plants. Ethno botanical data was recorded by opting people participation and key informant approach involving semi-structured interviews, group discussions and filling of questionnaires. The results showed a total of 114 medicinal plants belonging to 50 families were identified. Zingiberaceae was the most-frequently cited (nine species), followed by Asteraceae, Euphorbiaceae, and Fabaceae (seven species each). The most-used plant parts were leaves (51.8%), followed by stems (22.9%) and the most common preparations were decoction, poultice and squeezed. Most of the plants were obtained from the house-yard and total of 30 medicinal uses were recorded. The ethnobotanical result documented in this study showed that this area is rich in medicinal plants and these plants are still commonly used for medicinal purposes among the people in their daily lives. Ethnobotanical heritage should be preserved, however, there is a gradual loss of traditional knowledge about these plants in new generation. Further, the findings can be used as baseline information for further scientific investigation for analyzing phytochemical, pharmaceutical and other biological activities for future drug discovery.
PENGARUH RENDAMAN KAYU ANGIN (Usnea baileyi) DALAM NIRA SEGAR DAN NIRA REBUS TERHADAP HATI TIKUS (Rattus norvegicus) YANG TERPAPAR TIMBAL ASETAT Malini, Desak Made; Supartinah, Iin; Madihah, Madihah; Perdana, Andi
Pro-Life Vol 6 No 3 (2019): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Key words: lead acetate, liver, sap, Usnea baileyi