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Journal : e-Journal BUDIDAYA PERAIRAN

PENGGUNAAN RAGI ROTI (SACCHAROMYCES CEREVICIAE) SECARA IN SITU UNTUK MENINGKATKAN RESPON KEBAL NON-SPESIFIK IKAN NILA (OREOCHROMIS NILOTICUS) Rawung, Meilina E; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.2.2014.4901

Abstract

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus
IDENTIFIKASI PENYAKIT BAKTERIAL PADA BENIH SIDAT (ANGUILLA MARMORATA) DI BALAI BUDIDAYA AIR TAWAR TATELU Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6935

Abstract

This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
EFIKASI EKSTRAK TANAMAN OBAT BINAHONG ANREDERA CORDIFOLIA (TEN) STEENIS UNTUK MENINGKATKAN PERTUMBUHAN IKAN NILA OREOCHROMIS NILOTICUS Wantah, Monika M.; Longdong, Sammy N.J.; Kreckhoff, Reni L.; Mantiri, Desy M.H.; Tumbol, Reiny A.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20502

Abstract

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
PENGGUNAAN BAKTERI PROBIOTIK UNTUK PENCEGAHAN INFEKSI BAKTERI STREPTOCOCCUS AGALACTIAE PADA IKAN NILA, OREOCHROMIS NILOTICUS Umasugi, Aisin; Tumbol, Reiny A.; Kreckhoff, Reni L.; Manoppo, Henky; Pangemanan, Novie P.L.; Ginting, Elvi L.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20556

Abstract

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed.  Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus  agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of  survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria,  nile tilapia,  Streptococcus  agalactiae, growth,  infection
RESPON IMUN BENIH IKAN MAS, CYPRINUS CARPIO, YANG DIBERI PAKAN PROBIOTIK LACTOBACILLUS SP. DENGAN KONSENTRASI BERBEDA Pangalila, Novelia; Manoppo, Henky; Tumbol, Reiny A.; Lumenta, Cyska; Kreckhoff, Reni L.; Warouw, Veibe
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27492

Abstract

The research aimed to isolate probiotic bacteria from catfish intestine and assign the concentration of probiotic bacteria which effectively increase the activity of phagocytosis and total leukocytes of carp fingerlings. The fish was taken from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Fish (average initial weight of 2.27 g) were acclimated for one week in 15 aquaria with a density of 25 fish/aquarium. One week after acclimatization, fish were fed with food added with probiotics with different concentrations, namely 1x106, 1x107, 1x108, 1x109 cfu/mL for four weeks. The dose of feed was 5% /body weight/day and feeding time was at 08.00 and 17.00. Data collected were phagocytosis and total leukocyte count. The results showed that supplementation of probiotic significantly increased phagocytic activity and total leukocytes (p<0.01). The best phagocytic activity and total leukocytes were achived in fish fed diet added with probiotics 1x108 cfu/mL. It can be concluded that supplementation of  probiotic onto feed can increase phagocytic activity and total leukocytes of carp.
RESPON KEBAL NONSPESIFIK IKAN MAS YANG DIBERI IMUNOSTIMULAN RAGI ROTI SECARA ORAL Yanuar, Aditya P.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 5, No 2 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.2.2017.16547

Abstract

The effect of baker?s yeast as immunostimulant on innate immunity of carp (Cyprinus carpio L) had been investigated.  Fish was gathered from Freshwater Aquaculture Board (BBAT) Tatelu, Province of North Sulawesi.  The fish were acclimatized for a week in 15 aquariua with a density of 25 fish/aquarium. After acclimatization the fish were fed pellet supplemented with baker?s yeast as immunostimulant at 0, 5, 10, 15 and 20 g / kg of feed for four weeks as much as 5%/body weight of fish/day with the frequency of feeding twice a day at 08.00 am and 16.00 pm . This study used Completely Randomized Design (RAL) with five treatments and each treatment had three replications. Immune parameters consisting of total leucocytes and phagocytosis activity were measured in the last week. After  four weeks of feeding, the total leukocytes from the carp fed with the addition of baker?s yeast 10 g / kg of feed were significantly increased compared with the fish not being fed with the addition of bread yeast (p <0.01). Phagocytic activity of phagocyte cells from carp also increased significantly in fish fed pellet with the addition of 5 g of baker?s yeast / kg of feed (p = 0.01). In conclusion, the addition of baker?s yeast as immunostimulant in the diet at 5-10 g/kg of pellet can increase the nonspecific immune response of carp. Keywords:  baker?s yeast, carp, nonspesific immune response, total leucocytes, phagocytosis activity
PENINGKATAN RESPON KEBAL NON-SPESIFIK DAN PERTUMBUHAN IKAN NILA (OREOCHROMIS NILOTICUS) MELALUI PEMBERIAN JAHE, ZINGIBER OFFICINALE Payung, Clara Nunia; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6925

Abstract

The purpose of this research was to evaluate the effect ginger supplemented in food on nonspecific immune response of nile tilapia.  Juveniles with an average of weight of 27.31 g were obtained from Fish Culture and Development Board  (BP3I) Tateli and then transported to Faculty of Fisheries and Marine Science.  Fish were then cultured in 15 aquaria with a density of 15 fish per aquarium. Each aquarium was equipped with an aerator and used water recirculation system to keep the water quality still in good condition. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed as long as four weeks at 3%/bw/d, twice daily at 08.00 am and 17.00 pm.  Data consisting of total leucocyte count and phagocytosis activity of phagocyte cells were collected at two weeks interval. Research results showed that supplementation of ginger powder into fish pellet  had significant effect on the increase of nonspecific immune response. The highest total leucocyte count and phagocytosis activity of phagocyte cells was observed on fish fed pellet supplemented with 7.5 g/kg of food and significantly different as compared to those of control fish.  As conclusion, supplementation of ginger powder into fish pellet could increase nonspecific immune response on nile tilapia. Keywords: Ginger, nonspecific immune response, total leucocytes count, phagocytosis activity, nile tilapia
ISOLASI BAKTERI PROBIOTIK LACTOBACILLUS SP DARI USUS IKAN MAS (CYPRINUS CARPIO) Novitarizky, Intan A.; Manoppo, Henky; Longdong, Sammy N.J.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20492

Abstract

The objective of research was to isolate the probiotic bacteria from intestine of carp.  Isolation was performed by weighing 1 g of intestine, crushed and inserted in a reaction tube containing 9 mL of NaCl then centrifuged. Supernatant was taken and spread over the agar medium by scatter method. The bacteria-stocked medium was then incubated in an incubator at 28°C for 24-48 hours. The growing colonies were re-isolated on the agar medium to obtain pure colonies. The bacteria were then identified by morphological observation, resulting pigment and gram staining. Five nile tilapia (sized 8 ? 10 cm) were used in pathogenicity test and placed in the aquarium. Fish were injected with 0.2 ml of Lactobacillus solution containing 1x107 CFU/mL via intraperitoneal injection. The results showed that the bacteria Lactobacillus sp had rod-shaped characteristics, white pigment and gram positive and  not pathogenic, which means it can be used as probiotic for aquaculture.Keywords : Lactobacillus sp, carp, probiotic, pathogenicity test
RESPON IMUN KRUSTASE Manoppo, Henky; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.2.2014.4904

Abstract

Crustacean does not have adaptive immune system and mostly depends on innate or nonspecific immune response. This system can recognize and destroy foreign materials including pathogen.  The nonspecific immune responses of crustacean consist of cellular and humoral responses.  In cellular response, hemocyte plays an important role in body defenses to pathogen including virus, bacteria, fungi, and parasites. In humoral response, recognition of pathogen is mediated by protein and enzyme such as ?-1,3-glucan-binding protein (BGBP),  lipopolysaccharide-binding protein (LPS-BP), peptidoglycan recognition protein, phenoloxydase enzime. Keywords: crustacean, nonspecific immune response, cellular responses, humoral response, protein, enzime
PEMANFAATAN JAHE (ZINGIBER OFFICINALE ROSC) UNTUK MEMACU PERTUMBUHAN IKAN NILA (OREOCHROMIS NILOTICUS) Belseran, Lukas; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6930

Abstract

A research had been conducted to study  the effect ginger supplemented in feed on growth of nile tilapia.  Juveniles as much as 250 individuals  (mean weight 27.31 g) were obtained from Fish Culture and Development Board  (BP3I) Tateli. The fish were placed in plastic bags and transported to Faculty of Fisheries and Marine Science.  Fish were then introduced in 15 aquaria at a density of 15 individuals per aquarium. Each aquarium was equipped with an aerator and used water recirculation system. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed  treatment diets for four weeks at 3%/bw/day, twice daily at 08.00 am and 17.00 pm.  Growth data including absolute and relative growth were weighted at the end of feeding period. Research results showed that after four weeks of feeding, supplementation of ginger powder into fish pellet  significantly improved fish growth .  The growth of fish fed pellet supplemented with 7.5 g/kg of food was  and significantly different as compared to that of control fish.  It was concluded that supplementation of ginger powder into fish pellet potentially improved fish growth.   Keywords: Ginger, absolute growth,  relative growth, nile tilapia