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KEANEKARAGAMAN JENIS TUMBUHAN DAN PENDUGAAN CADANGAN KARBON DI ATAS PERMUKAAN TANAH DI KAWASAN HUTAN BUKIT BARISAN BAGIAN BARAT KOTA PADANG Yastori, .; Chairul, .; Syamsuardi, .; Mansyurdin, .; Maideliza, Tesri
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.212 KB) | DOI: 10.24843/metamorfosa.2016.v03.i02.p02

Abstract

Indonesia has a vast forest area. The extent of Indonesia's forests is one of the natural resources are prone to damage due to human interests in meeting their needs. One of the damage that often occurs when current is forest fires. Forest destruction accounts for 20-25% of global CO2 emissions that contribute to climate change or global warming. Unspoiled forest with a diversity of plant species are long-lived and litter is a place to store a lot of carbon stocks (C) the highest. The aim of this study was to determine the diversity of plants and the amount of carbon stock above ground level in the forests of the Bukit Barisan of Padang, West Sumatra. Tree biomass was calculated on a plot of 20x20 m, 10x10 m pole, stake 5x5 m, for counting down plant biomass and litter on the plot with a size of 2x2 m (National Standardization Agency, 2011). Biomass calculated by the Ketterings et al. formula (2001). In Bukit Barisan Forest Area, West Sumatra, derived carbon content was 16.029,70 ton/ha. Diversity type was highest at tree level on Station 1, classified as very high diversty with diversity index (H?) 3.10.
Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall ISDA, Mayta Novaliza; KASIM, Musliar; MANSYURDIN, .; CHAIDAMSARI, Tetty; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.457 KB) | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.
STUDI LINGKAR TUMBUH POHON DI KAWASAN HUTAN TAMAN NASIONAL SIBERUT KEPULAUAN MENTAWAI Mansyurdin, .; Maideliza, Tesri; Chairul, .; Susiana, Ema
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.034 KB) | DOI: 10.24843/metamorfosa.2016.v03.i01.p02

Abstract

Growth ring trees are formed by activity of the cambium which is influenced by the changing seasons.   In the tropical are not all of  trees species produce  the growth ring, because the season of  tropics is more uniform throughout the year and does not show sharp distinction between the periods of high rainfall and period of low rainfall. This study has concentred on several tree forest areas in Siberut National Park, Mentawai Islands. Samples were ollected by using borer on the main stem on the height of 130 cm. To see or not to see growing circle with to be checked macroscopically and microscopically. The macroscopic examination was done polished core by several grades of sandpaper in the transverse surface Tree species which have growth ring continued to microscopic observation with making slice anatomy. Based on 46 species of trees were examined, and  6 species were with found a growth ring in the number of cell mixture early wood and late wood from these species.
STRUKTUR KERAPATAN VEGETASI DAN ESTIMASI KANDUNGAN KARBON PADA BEBERAPA KONDISI HUTAN DI PULAU SIBERUT SUMATERA BARAT Chairul, .; Muchktar, Erizal; Mansyurdin, .; Maideliza, Tesri; Indra, Gusmardi
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (454.672 KB) | DOI: 10.24843/metamorfosa.2016.v03.i01.p03

Abstract

Hutan Tropik memiliki sumber cadangan karbon sangat besar yang dapat berubah akibat perubahan kondisi hutan yang disebabkan oleh aktivitas  manusia, termasuk penebangan dan perkebunan sehingga mengancam perubahan iklim dunia. Kajian analisis dilakukan untuk menetahu struktur vegetasi dan kandungan karbon pada tiga kondisi hutan di Pulau Siberut sebagai upaya mitigasi perubahan iklim. Untuk mendapatkan data biomassa bagian atas tanah dan serasah digunakan metode plot berpetak.  Hasil Penelitian didapatkan 38 spesies dan 122 individu tumbuhan pada hutan primer, 22 spesies dan 49 individu pada hutan bekas tebangan serta 45 spesies dan 120 individu pada hutan tanaman campuran. Kandungan karbon bagian atas tumbuhan hidup pada hutan primer 1.359.884,68 kg/ha, hutan bekas tebangan 610.429,67 kg/ha  dan hutan tanaman campuran 360.793,70 kg/ha.  Kandungan karbon pada serasah hutan primer 774,49 kg/ha, hutan bekas tebangan 521,36 kg/ha dan hutan tanaman campuran 766,20 kg/ha.
KAJIAN ANATOMI KAYU PADA TIGA EKOTIPE PINUS MERKUSII SUMATERA DAN POTENSINYA SEBAGAI INDIKATOR PERUBAHAN IKLIM Sandri, Yulia; Maideliza, Tesri; Mansyurdin, .; Febriamansyah, Rudi
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (697.788 KB) | DOI: 10.24843/metamorfosa.2016.v03.i02.p07

Abstract

Recently, climate change is the one of most important environmental issue. Climate variability can be recorded by tree growing through the growth ring. Growth ring formed by cambial activity were examined in wood anatomy. In Sumatra, there are three ecotypes Pinus merkusii, namely ecotypes Kerinci, Tapanuli, and Aceh which can be distinguished morphologically. This study aims to knowing the wood anatomical characteristics of the three ecotypes and determine the potential as climate indicator. This study was conducted in October 2014 until June 2015. Sample of Kerinci ecotype was collected in Kerinci Seblat National Park, Tapanuli ecotype in Dolok Sibualbuali Natural Reserve and Aceh ecotype in Gunung Leuser National Park on a height of 130 cm using increment borer and cut on the main stem 5×5 cm for anatomical sample. Results from this study indicate that ecotype Kerinci and Tapanuli showed earlywood and latewood boundary exposing the clear growth ring, whereas in Aceh ecotype unclear. Tapanuli ecotype have the thickest tracheid diameter than ecotype Kerinci and Aceh. Ecotypes of Kerinci, Tapanuli, and Aceh has homoceluler and uniseriate ray where Aceh ecotype have the longest ray. Furthermore, Kerinci and Tapanuli ecotype have potential as climate indicator eventhough showed negative correlation, that Tapanuli ecotype show the best result and recommended in dendrochronology study.