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ISOLATION AND CHARACTERIZATION OF SILAFFIN THAT CATALYZE BIOSILICA FORMATION FROM MARINE DIATOM CHAETOCEROS GRACILIS MANURUNG, AGNES IMELDA; PRATIWI, ALBERTA RIKA; SYAH, DAHRUL; SUHARTONO, MAGGY THENAWIDJAJA
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.311 KB) | DOI: 10.4308/hjb.14.3.119

Abstract

The method of making silica in industries requires extreme conditions. The finding of proteins involved in the formation of biosilica from diatoms, has opened up an alternative way of production. Chaetoceros gracilis is one of the diatoms, which is potential in producing silaffin protein. This study aimed to isolate and to characterize the protein. We also analyzed the protein activity toward tetraethoxyorthosilicate (TEOS) substrate in in vitro reaction. Diatom biomass was harvested and further kept in 2% SDS/100 mM EDTA solution. Protein isolation was conducted by dissolving the silica and separating the protein by soaking in 2 M HF/8 M NH4F. Protein concentration was analyzed using Bradford method and the molecular weight was estimated through SDS-PAGE. Protein activity was observed by reacting it with TEOS substrate to form silica polymer and measured by colorimetric molibdate assay. Protein concentration was 1.20 mg/ml and appeared filamentous. The apparent molecular weights consisted of 12, 23, 42, 44 kDa. These protein was able to polymerize the silica at room temperature within 10 min. As much as 85.65 umol TEOS was polymerized per 1.4 x 106 silaffin protein per min. SEM analysis showed the formation of spherical, aggregate biosilica. Key words: Chaetoceros gracilis, silaffin protein, biosilica, polymerization
PEMBERIAN PUPUK NITROGEN DAN PUPUK ORGANIK GRANUL TERHADAP PERTUMBUHAN DAN PRODUKSI BAWANG MERAH (ALLIUM CEPA L.) Manurung, Agnes Imelda; Sirait, Bilter A; Hulu, Tonasokhi; Marpaung, Robbert G
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 1 (2019): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Desember
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.143 KB) | DOI: 10.31289/agr.v4i1.2750

Abstract

This study aims to determine the response of growth and  production  of  shallot  (Allium cepa L.) to the Nitrogen fertilizer and Granule Organic fertilizer. The design used was a Factorial Completely Randomized Design consisting with two (2) treatment factors. The first factor is Nitrogen (N) from Urea which consists of three levels, namely: N1 = 2 g / plot, N2 = 4 g / plot and N3 = 6 g / plot. The second factor of Organic Granule fertilizer consisted of three levels, namely: G1 = 5 kg / plot, G2 = 10 kg / plot, G3 = 15 kg / plot. The results showed that interaction of Nitrogen fertilizer  and Granul Organic fertilizer significantly affected plant height, number of leaves, number of tillers and tubers wet weight per plot, but not significant for the number of flower stalks. The highest growth and production of shallots produced a combination of treatment N3 = 6 g / plot and G3 = 15 kg / plot.
PRELIMINARY RESEARCH THE EFFECT OF NAA AND BAP ON CALLUS OF BANANA (MUSA ACUMINATA L) WITH ADDITION OF FUSARIUM WILT DISEASE FROM FUSARIUM OXYSPORUM F.SP.CUBENSE (FOC) Sirait, Bilter A.; Napitupulu, Nur Synta; Manurung, Agnes Imelda
JURNAL DARMA AGUNG Vol 26 No 3 (2018): Desember
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Darma Agung (LPPM_UDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.242 KB)

Abstract

THE EFFECTS OF NAA AND BAP ON Callus OF BANANA (Musa acuminata L)  WITH THE ADDITION OF FUSARIUM WILT DISEASE FROM Fusarium oxysporum f.sp.cubense (Foc).  This research was conducted on January to June 2016, at Tissue Culture Laboratory of Dinas Tanaman Pangan dan Hortikultura of North Sumatera Province, Jl.Karya Jasa No.6 Gedung Johor, Medan. The purpose of this research is to know the response of banana barangan callus (Musa acuminate L.) to fusarium (Fusarium oxysporum f. Sp.Cubens). The living callus is subsequently subcultured for organogenesis purposes by treatment of NAA and BAP. The research use Completely Random Design with two factors that is NAA and BAP. NAA consists of 3 levels: N1 = 1 ppm, N2 = 1.5 ppm and N3 = 2 ppm. The second factor using BAP consists of 3 levels: BA1 = 1.5 ppm, BA2 = 3 ppm and BA3 = 3.5 ppm. The results showed that the percentage of live callus after fusarium addition was only 33.33%, interaction of NAA and BAP did not significantly affect the percentage of live plantlets and wet weight of banana plantlet. The highest percentage of live plantlet on aged 9 - 13 week after subculture and the highest wet weight of planlet found on N1(1 ppm) each 7.05 % and 3.62 g, while the highest percentage of live plantlets on aged 9 - 13 Week after subculture and the highest wet weight of plantlet found on BA1 (1,5 ppm) each 5.69 % and 3.38 g. The treatment combinations that gave the highest percentage of planlets live and wet weight of planlet were N1BA1 and N1BA2.