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IDENTIFIKASI DAN PREVALENSI EKTOPARASIT PADA IKAN NILA (OREOCHROMIS NILOTICUS) DI KOLAM BUDIDAYA KAMPUNG HIUNG, KECAMATAN MANGANITU, KABUPATEN KEPULAUAN SANGIHE Manurung, Usy N; Gaghenggang, Fatmawati
e-Journal BUDIDAYA PERAIRAN Vol 4, No 2 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.4.2.2016.13053

Abstract

A research had been conducted to identify and analyze the prevalence of ectoparasites on Nile tilapia (Oreochromis niloticus) cultured in Kampung Hiung, Manganitu District of Sangihe Island Regency during May 2016. Identification was conducted at The Laboratory of Fisheries and Maritime, Polytechnic of Negeri Nusa Utara.  The research found five parasite species infecting nile tilapia including Dactylogyrus sp, Oodinum sp, Lerneae sp, Gyrodactylus sp and Trichodina sp. The highest prevalence value was Dactylogyrus sp (86.67%), followed by Oodinium sp (33,33 %), Lerneae sp (13,33%), Gyrodactylus sp (6.67%) and Trichodina sp (6.67%) Keywords:  parasite, identification, prevalence, Oreochromis niloticus
EVALUATION OF BAKER’S YEAST (SACCHAROMYCES CEREVICIAE) IN ENHANCING NON SPECIFIC IMMUNE RESPONSE AND GROWTH OF NILE TILAPIA Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.1.2013.720

Abstract

This research was conducted to evaluate the efficacy of baker?s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker?s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker?s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker?s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker?s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker?s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker?s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
THE USE OF BAKER’S YEAST (SACCHAROMYCES CEREVICIAE) AS IMMUNOSTIMULANT TO ENHANCE RESISTANCE OF NILE TILAPIA (OREOCHROMIS NILOTICUS) TO AEROMONAS HYDROPHILA Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 2 (2014): Oktober
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2014.7300

Abstract

The objective of this research was to evaluate the efficacy of Baker?s Yeast S. cereviciae in enhancing the resistance of nile tilapia (Oreochromis niloticus) to A. hydrophila. As many as 250 fish with an average weight of 28.78±2.44 g were obtained from Fish Culture Development and Training Center of Tateli. After acclimatization, the fish were fed pellet supplemented with Baker?s Yeast as treatments at five different doses, A=0 gr/kg feed, B=5 gr/kg feed, C=10 gr/kg feed, D=15 gr/kg feed, and E=20 gr/kg feed each of which was with three replications.  They were fed for four weeks at 5%/BW/day, twice a day at 08.00 and 16.00, respectively. After feeding period, the fish were challenged intraperitoneally with A. hydrophila.  Before injection, a pathogenicity test of bacteria A. hydrophila was conducted for LD50. Challenged test was carried out by injecting fish with 0.2 ml of bacterial suspension containing 5 x 106 cfu/ml. The fish resistance was observed for 14 days. Dead fish were taken out and bacterial isolation was performed to confirm the cause of the dead.  Results showed that supplementation of Baker?s Yeast into fish pellet had significant effect  on the fish resistance (p=0.00).  The highest resistance (66.6%) was recorded in fish fed with pellet supplement of 5 g Baker?s Yeast per kg of pellet while control fish was only 50%.  As conclusion, supplementation of Baker,s Yeast into fish pellet could enhance resistance of fish to the pathogen. Penelitian ini bertujuan untuk menguji efikasi ragi roti S. cereviciae dalam meningkatkan resistensi ikan nila (Oreochromis niloticus)terhadap bakteri patogen A. Hydrophila. Sebanyak 250 ekor ikan nila dengan berat awal rata-rata 28,78±2,44 g yang diambil dari Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Setelah aklimatisasi, ikan diberi ragi roti sebagai perlakuan dengan lima dosis berbeda dan masing-masing perlakuan memiliki tiga ulangan.Perlakuan ragi roti yang digunakan adalah A=0 gr/kg pakan, B=5 gr/kg pakan, C=10 gr/kg pakan, D=15 gr/kg pakan, E=20 gr/kg pakan. Lama pemberian pakan perlakuan empat minggu dengan dosis 5%/bb/hari dan diberikan 2 kali sehari yaitu Pukul 08.00 dan Pukul 16.00. Setelah diberikan ragi roti selama empat minggu ikan diuji tantang dengan bakteri A. Hidrophyla secara Intraperopeneal (ip). Sebelum penyuntikan, dilakukan uji patogenitas bakteri yang memberikan tingkat kematian 50% (LD50). Uji tantang dilakukan dengan cara menyuntikkan 0,2 ml suspensi bakteri pada kepadatan 5 x 106 cfu/ml (sesuai hasil uji patogenitas) pada rongga tubuh ikan. Pengamatan resistensi ikan akan dilakukan setiap hari selama 14 hari. Ikan mati dikeluarkan dan dilakukan isolasi bakteri untuk mengkonfirmasi penyebab kematian ikan. Hasil penelitian mendapatkan bahwa pemberian ragi roti berpengaruh sangat nyata (P=0,00) pada resistensi ikan terhadap bakteri A. Hydrophila. Resistensi tertinggi dicapai pada ikan yang diberi perlakuan B (5 g/kg pakan) dengan tingkat resistensi mencapai 66,6%. Sedangkan ikan yang tidak diberi perlakuan ragi roti (control) memiliki resistensi 50 %. Sebagai kesimpulan penambahan ragi roti dalam pakan dapat meningkakan resistensi ikan terhadap infeksi pathogen.
Identifikasi Ektoparasit pada Ikan Mas Koi (Cyprinus Carpio Koi) di Kolam Budidaya Kampung Hiung, Kecamatan Manganitu Kabupaten Kepulauan Sangihe Takasiliang, Diknawati; Manurung, Usy N
Jurnal Ilmiah Tindalung Vol 2 No 2 (2016): Jurnal Ilmiah Tindalung
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, POLITEKNIK NEGERI NUSA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.214 KB) | DOI: 10.5281/jit.v2i2.89

Abstract

Parasit yang menginfeksi ikan budidaya dapat mengakibatkan menurunnya produksi bahkan kematian masal. Dalam jumlah sedikit, parasit yang menginfeksi masih dapat ditolerir oleh inang, tetapi dapat menyebabkan gangguan metabolisme bahkan kerusakan organ jika terjadi dalam intensitas yang tinggi. Penelitian ini bertujuan untuk mengidentifikasi ektoparasit pada ikan Mas Koi (Cyprinus carpio koi) di Kolam Budidaya Kampung Hiung, Kecamatan Manganitu Kabupaten Kepulauan Sangihe. Identifikasi dilakukan di Laboratorium Perikanan dan Kebaharian Politeknik Negeri Nusa Utara. Berdasarkan hasil identifikasi yang telah dilakukan didapatkan parasit, Dactylogyrus sp dan Lerneae sp, sedangkan nilai prevalensi tertinggi parasit yang menyerang ikan mas koi adalah Dactylogyrus sp yaitu sebesar 100% dan Lerneae sp 10%.