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SELEKSI DAN UJI ANTIBAKTERI AKTINOMISETES ASAL TANAH GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Escherichia coli DAN Salmonella typhi Suryani, Siti; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Actinomycetes is one of microorganisms that can be  used as a natural antibiotic. Metabolites produced by actinomycetes have antagonistic activity on bacteria, therefore it can be used as antibacteria. The purposes of this study were to select and test the antibacterial activity of actinomycetes isolated from peat soil in Desa Rimbo Panjang Kabupaten Kampar Riau to inhibit the growth of Escherichia coliand Salmonella typhi. This study was conducted from December 2013-January2014 at the Microbiology Laboratory, University of Riau. Serial dilutions and pour plate method used for isolation of actinomycetes while agar disk method used for antibacterial test. The result found a total of 24 actinomycetes isolates, 10 isolates inhibiting  E. coli, and 16 isolates inhibiting S. typhi. The inhibition zone diameter of E. coliand S. typhi  were 8,3 to 16,9 mm and 7,8 to 12,9 mm, respectively.
ANALISIS FISIOLOGI JAMUR LIGNINOLITIK DAN SELULOLITIK ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Larasati, Karina; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cellulolytic and ligninolytic fungi have an important role in degradation of organic materials, especially lignin and cellulose, in a composting process. The success of the fungi in the composting process is strongly influenced by temperature and pH. The purpose of this research was to obtain isolates of the fungi with the best ability to be applied as biocompost agent by analyzing its ability to grow in different temperature and pH. A total of 110 isolates from peatsoil in Rimbo Panjang, Kampar Regency, Riau was inoculated into Potato Dextrose Broth medium with pH of 5 and pH of 7 then incubated for 3 days at room temperature (±27ºC), 40ºC, 50ºC, 60°C, 70ºC and 80ºC. The results obtained 110 isolates which were grown at room temperature (±27ºC) with pH of 5 and pH of 7 and 96 isolates which were grown at 40ºC temperature. A total of 27 isolates which were grown at 50ºC temperature consisted of genus Penicillium, Trichoderma, and Aspergillus.
UJI EFEKTIVITAS JAMUR Metarhizium anisopliae Cps.T.B ISOLAT LOKAL TERHADAP RAYAP (Coptotermes curvignathus) ', Rozalia; Martina, Atria; ', Titrawani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Termite is one of the major pest on oil palm plantations, especially in peat soil. Application of synthetic insecticides will cause resistant insect, enviromental pollution, and other problems. The entomopathogenic fungus Metarhizium anisopliae has been reported to infect many insects, including termite. This study was aimed to analyze the effectiveness of treatment of M. anisopliae Cps.T.B and M. anisopliae Cps.T.B with zeolit in controlling termite, C. curvignathus. This research used a completely randomized design with three treatments and eight replications. The result showed that spore density of M. anisopliae Cps.T.B with zeolite was 2,5 x108 spores/g and M. anisopliae Cps.T.B without zeolite was 3,2 x108 spores/gram respectively. Germination ability of M. anisopliae Cps.T.B with zeolite was 91.11% and M. anisopliae Cps.T.B without zeolite was 87.41% respectively. Mortality of C. curvignathus on eight days after treatment using M. anisopliae Cps.T.B was 100% and using M. anisopliae Cps.T.B with zeolite was 95,5% while the mortality of C. curvignathus a control was 15%.
UJI AKTIVITAS ANTIFUNGAL KULIT MANGGIS (Garcinia mangostana) DAN SEMANGKA (Citrullus vulgaris)TERHADAP Trichophyton mentagrophytes PENYEBAB DERMATOMYCOSIS Sholihah, Tengku Putri; Martina, Atria; ', Yuharmen
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Dermatomycosis is the most common infection diseasesuffered by inhabitants of tropical countries and caused by  Trichophyton mentagrophytes. Mangosteen and watermelon epicarp have high phytochemical compounds which is potential as antibacterial, antifungal and antioxidant. This study aimed to determine antifungal activity of mangosteen and watermelon epicarp against  Trichophyton mentagrophytes. The study was conducted using completely randomizedfactorial design. Epicarp of mangosteen and watermelon were processed to fresh and dry extracts with concentration 40% and 80% (fresh extract) and 5%, 10% and 20% (dry extract). Dry extract were dissolved using 3 different solvent, e.g: aquabides, heated aquabides and etyl alcohol. Antifungal activity was tested using paper disc method. The results showed that almost all of watermelon and mangosteen epicarp dry extract had antifungal activity againts  Trichophyton mentagrophytes,  while fresh extract did not showed antifungal activity againts  Trichophyton mentagrophytes.  The highest antifungal activity of watermelon was showed in 20% extract in heated aquabides with inhibition zone diameter is 36,8 mm. The highest antifungal activity of mangosteen was showed in 10% extract in heated aquabides with inhibition zone diameter is 28,5 mm.
PENGENDALIAN Ganoderma boninense OLEH Trichoderma sp. SBJ8 PADA KECAMBAH DAN BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) DI TANAH GAMBUT Alviodinasyari, Rizky; Martina, Atria; Lestari, Wahyu
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Basal stem rot disease caused by Ganoderma boninense is the most destructive disease in oil palm plantation in Indonesia, especially in the peatland. The use of chemical pesticides as controlling agent is hazardous to environment and human health. The alternative to control this disease is by using biological agent Trichoderma sp. SBJ8 in the form of Ganofend biofungicide. The research were aimed to study the effectivity ofTrichoderma sp. SBJ8 isolate in suppressing in vitro growth of G. boninense, as well as to determine the ability of Ganofend biofungicide in inhibiting the growth of G. boninense on the oil palm germination and seedling. The experimental study included two cultures i.e. in vitro and in vivo using randomized design with 6 treatments: without Ganofend biofungicide treatment, 100 g Ganofend biofungicide treatment, and with immersing in Ganofend biofungicide solution for both root and seedling of oil palm. The results showed that the isolates of Trichoderma sp. SBJ8 could inhibit the growth of G. boninense in 4th day up to 65,25%. The treatment using Ganofend biofungicide was effective to decrease the mortality percentage of oil palm germination and not causes seedling death.
Seleksi Aktinomisetes Penghasil Protease dari Tanah Gambut Desa Langkai, Siak, Riau LINDA, TETTY MARTA; MARTINA, ATRIA; FEBRIANTI, BERNADETA LENI; , HERLINDA; , TABRI
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Actinomycetes is well known as secondary metabolites producer and hence it has high pharmacological andcommercial interest. In this study, a total of 14 isolates of actinomycetes from peat soils in Langkai Village,Siak, Riau had been selected for their ability to produce protease. Extracellular protease from Actinomycetesisolates was characterized by incubating the isolate in Nutrient Agar media containing casein 1%. Screeningof actinomycetes isolates showed that five isolates had the ability to produce a clear zone with the value ofproteolytic index (IP) ranged from 5.73 to 11.15. Isolates L313 showed the highest IP value (11.15), as wellas the highest protease production (0.041 U/ml) in Nutrient Broth media containing casein 1% after 6 daysincubation at room temperature.Key words: actinomycetes, clear zone, peat soil, protease, proteolitic index
Uji Patogenisitas Fungi Entomopatogen Lokal Riau sebagai Agen Biokontrol Hama Rayap (Coptotermes curvignathus Holmgren) SINTAWATI, REGIANA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Biopesticide is a technique that can be used to the control pest population in the environment. Thepurpose of this research was to isolate fungi from the soil using baiting and cadaver technique andpathogenicity test. Isolates which had high germinating conidia was tested their patogenicity againstCoptotermes curvignathus. A total of 12 isolates were baited from soil and cadaver samples at palm oilplantation. The result showed eight isolates had high germinating conidia categories (˃ 80%). Thepathogenicity test of four isolates showed 100% mortality for RB.r01 and BK.r01; and KB.r01 andNK.r01were 72% and 67% respectively. Isolates capability against termite was not significantly different,but it was significantly different from control.Keywords : entomopathogenic fungi, pathogenicity, Coptotermes curvignathus, biocontrol
UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Martin, Dede; Martina, Atria; Roza, Rodesia Mustika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.
Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid) MAWARTI, INDAH; FIBRIARTI, BERNADETA LENI; ZUL, DELITA; ROZA, RODESIA MUSTIKA; MARTINA, ATRIA; LINDA, TETTY MARTA
Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.
UJI EFEKTIVITAS JAMUR Metarhizium anisopliae Cps.T.A ISOLAT LOKAL TERHADAP HAMA RAYAP (Coptotermes curvignathus) ', Khairunnisa; Martina, Atria; ', Titrawani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Termites is one of the major pest of oil palm plantations on peatlands. Termite control is generally performed using chemical insectisides. Several chemical insecticides can inhibit termite attack effectively but not effeciently used because it has many negative effects. The negative effect of chemical insecticides can be reduced using biological agents such as entomopathogenic fungus. This research aimed to determine the effectivenes and the best formulation of local isolate of M. anisopliae Cps.T.A and with the addition of 10% zeolite in controling C. curvignathus. The reaserch used Complete Randomized Design (CRD) with three treatments and eight replications. The results showed that density of M. anisopliae Cps.T.A with addition of 10% zeolite was 2,25 x 108 spora/g and M. anisopliae Cps.T.A was 2,50 x 108 spora/g. Germination of M. anisopliae Cps.T.A with 10% zeolite was 91,2% and M. anisopliae Cps.T.A was 88,1%. The treatment of M. anisopliae Cps.T.A produced the highest mortality (100%) of M. anisopliae Cps.T.A with 51% zeolite, whereas the mortality of termite control was only 16%.