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SISTEM MODUL MIKROFILTRASI DALAM PEMURNIAN L-THEANINE DARI TEH HIJAU LOKAL (Camellia assamica) Pekoe UNTUK MINUMAN FUNGSIONAL Susilowati, Agustine; Aspiyanto, Aspiyanto; Melanie, Hakiki; Maryati, Yati
Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Vol 1, No 1 (2010): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 1 2010
Publisher : Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik

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Abstract

Microfiltration (MF) module system had a potential use in purifying of L-Theanine of local green tea (Camellia assamica) grade Pekoe for functional drink as anti stress compound. Purification process was performed through MF membrane of 0.2 µm at pump motor frequency of 20 Hz, room temperature, and operation pressure of 4 and 6 bar for 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300 minutes. The experiment result showed that MF system was able to pass more much L-Theanine in permeate, but retained more much dry matter and dissolved protein in retentate. Long purification time would decrease permeate flux value and L-Theanine concentration in permeate, but increased dry matter and dissolved protein in retentate. Optimum operation condition based on the highest concentration of L-Theanine in permeate at operation pressures of 4 and 6 bar for 120 and 240 minutes was able to pass L-Theanine in permeate of 86.33 % and 71.21 from all L-Theanine prior to process. Permeate as source of L-Theanine at optimum condition gave permeate flux values of 13.72 and 15.55 L/m2.hour with concentrations of L-Theanine of 10.721 % and 10.203 % (dry weight), dissolved protein of 2.1 and 1.8 mg/mL, and   dry matter of 1.342 and 1.712 %. Retentate has a potential use as functional compounds for anti oxidant,  anti cancer, anti diabetes and anti cholesterol. Key Words : Camellia  asssamica, L-Theanine, Microfiltration (MF), permeate, retentate.
PENGEMBANGAN DAN MODIFIKASI SISTEM PENGUKURAN SUSCEPTIBILITAS DAN PERMEABILITAS BAHAN MAGNET MARYATI, YATI; RUDIYANTO, HERLAN; APRILIA, ANNISA; SARAGI, TOGAR
Jurnal Material dan Energi Indonesia Vol 8, No 2 (2018): Jurnal Material dan Energi Indonesia
Publisher : Departemen Fisika Fakultas MIPA Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.896 KB) | DOI: 10.24198/jmei.v8i2.19902

Abstract

Telah dilakukan pengukuran suseptibilitas dan permeabilitas bahan menggunakan prinsip resonansi pada rangkaian RLC dengan solenoida sebagai induktor. Beberapa faktor yang mempengaruhi kuat induksi magnetik solenoida meliputi arus listrik, panjang solenoida, jumlah lilitan dan bahan yang disisipkan kedalam bagian dalam solenoida. Pada penelitian ini dilakukan modifikasi kumparan solenoida untuk mengetahui pengaruh jenis kumparan terhadap respon medan magnet yang dihasilkan. Kumparan solenoida yang digunakan memiliki jumlah lilitan N = 500 dan 1000. Bahan sisipan yang digunakan pada penelitian ini adalah besi, aluminium dan tembaga. Hasil pengukuran dengan modifikasi solenoida menunjukkan kesesuaian dengan teori yang ada
THE EFFECT OF PROCESS CONDITIONS IN PREPARATION OF VEGETABLE BROTH AS SAVORY FLAVOR FROM MUNG BEANS (PHASEOLUS RADIATUS L.) USING INOCULUM OF RHIZOPUS-C1 Susilowati, Agustine; Aspiyanto, Aspiyanto; Melanie, Hakiki; Maryati, Yati
Indonesian Journal of Chemistry Vol 8, No 3 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21592

Abstract

Preparation of vegetable broth from mung beans (Phaseolus radiatus L.) in semi pilot scale is an attempt development to get product of savory flavor in larger scale. The aim of this activity was to find out the effect of process multiplication on composition of vegetable broth from mung beans using inoculum of Rhizopus-C1 through brine fermentation in mixtures of inoculum, salt, and mung beans of 26, 23, and 51 %. This activity was conducted in both temperature of fermentation (room temperature and 30 °C), various time of fermentation (0, 2, 4, 6, 8, 10, and 12 weeks) and process scales, namely laboratory scale (300 g) and semi pilot scale (± 25 kg), respectively. The result of experiment indicated that fermentation temperature and time and process scales were tend to affect on composition of product. The length of fermentation time would increase concentrations of dissolved protein, N-amino and reducing sugar, decreased concentrations of fat and Volatile Reduction Substance (VRS), while concentrations of total protein, and water were tend to be constant in laboratory and semi pilot scales at the both process temperatures. Multiplication in preparation process of inoculum (7 kg) using starter of Rhizopus-C1 resulted inoculum with activities of proteolytic of 0.71 U/g, and amilolytic of 17.5 U/g at 56 h of incubation. The whole process in semi pilot scale decreased composition of products. The optimal treatment based on recovery of total protein, and the highest amino acids as N-amino in semi pilot scale was at fermentation temperature of 30 ºC for 10 weeks with concentrations of water of 44.96%, total protein of 11.77% (dry matter), dissolved protein of 8 mg/mL, N-amino of 15.4 mg/mL, reducing sugar of 582.5 mg/mL, fat of 0.26% and, VRS of 90 µeq.reduction/g.
PENGARUH KECEPATAN PUTAR DAN JENIS GEL SORGUM (Sorghum Bicolor L. Moench) DALAM PEMEKATAN PATI RESISTEN MELALUI SEL ULTRAFILTRASI BERPENGADUK UNTUK ANTI KOLESTEROL Susilowati, Agustine; aspiyanto, .; maryati, yati
Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Vol 1, No 1 (2011): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 2 2011
Publisher : Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik

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Abstract

Abstrak Pemekatan serat makanan (Dietary Fiber) dari sorgum Sorgum bicolor L. Moench) yang diperoleh dari ekstraksi secara kimia (gelatinisasi) melalui ultrafiltrasi ((UF) sel berpengaduk dimaksudkan untuk mengetahui pengaruh kecepatan putar pengaduk pada gel sorgum dari proses gelatinisasi dengan pH yang semakin meningkat terhadap komposisi konsentrat gel sorgum terutama SDF (Solouble Dietary Fiber) yang berfungsi sebagai pati resistan atau pati tak tercernakkan untuk anti kolesterol. Pemekatan dilakukan pada gel sorgum dari proses gelatinisasi dengan pH 10, 11, 12, 13 dan 14 menggunakan membran UF 30000 MWCO pada kecepatan putar 200, 300 dan 400 rpm dengan tekanan proses 30 selama 30 menit pada suhu ruang. Hasil penelitian menunjukkan bahwa semakin tinggi kecepatan putar pengaduk akan meningkatkan komposisi retentat pada tingkat alkalinitas gel yang semakin rendah. Kondisi proses optimal berdasarkan SDF tertinggi dicapai pada kecepatan putar 400 rpm selama 30 menit  pada gel dari proses gelatinisasi pada pH 10 dengan menghasilkan konsentrat sebagai pati tidak tercernakkan (resistance starch) dengan konsentrasi SDF, Tannin, Total Padatan, Serat kasar berturut-turut sebesar 5,62 % (b.k),  0,36% (b.k) , 0,79%, 1,19% dan IDF sebesar 2,635% (by difference).  Pada kondisi optimal ini dihasilkan perolehan kembali SDF sebesar 60,08% dari TDF sebesar 8,255% pada konsentrasi 30% pati kering whole grain atau rendemen sebesar 27,52% dari whole grain sorgum berdasarkan 100% pati kering. Kata kunci : sorgum, gelatinisasi, serat terlarut (SDF), kecepatan putar pengaduk, ultrafiltrasi. Abstract Dietary Fiber concentration from sorghum (Sorghum bicolor L. Moench) extract by gelatinization process (chemical method) through Stirred Ultrafiltration (UF) Cell was aimed to find out effect of rotation speed of stirred cell on gelatinized sorghum gel. pH value becoming more and more high on composition of sorghum gel concentrate, especially Total Dietray Fiber (TDF) and Soluble Dietary Fiber (SDF) functioned as resistance starch for anti cholesterol. Concentrating of gelatinized sorghum gel with pH of 10, 11, 12, 13 and 14 utilized UF membrane of 30,000 MWCO at stirrer rotation speeds of 200, 300 and 400 rpm, room temperature and operation pressure of 30 psia for 30 minutes. The experiment result indicated that rotation speed of high stirrer cell would increase retentate composition at low gel alkalinity level. Based on the highest SDF, optimal process condition was reached at rotation speed of stirrer cell of 400 rpm for 30 minutes using gelatinized gel at pH of 10 produced retentate as resistance starch with concentrations of  SDF, Tannin, Total Solids, Crude Fibre of 5.62 % (dry weight), 0.36 % (dry weight), 0.79 %, 1.19 %, and IDF of 2.635 % (by difference). At this optimal process condition was resulted a recovery of SDF of 60.08 % and TDF of 8.255 % at dry starch concentration (whole grain) of 30 % or or rendement of 27.52 % from whole grain sorghum based on 100 % of dry starch. Key Words : Sorghum (Sorghum bicolor L. Moench), dietary fiber (DF), gelatinization, soluble dietary fiber (SDF), ultrafiltration (UF).
SEPARATION OF FERMENTED INULIN FIBER BY Lactobacillus acidophillus USING Aspergillus clavatus-CBS5 THROUGH MICROFILTRATION MEMBRANE - (PEMISAHAN SERAT INULIN TERFERMENTASI OLEH L. acidophillus MENGGUNAKAN A.. clavatus-CBS5 MELALUI MIKROFILTRASI MEMBRAN) Aspiyanto, Aspiyanto; Susilowati, Agustine; Maryati, Yati; Melanie, Hakiki
Biopropal Industri Vol 8, No 2 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

The condition of Stirred Filtration Cell (SFC) was used as reference to a large-scale process conditions (modules) on the separation offermented inulin fibers by Lactobacillus acidophillus. Inulin hydrolyzate as biomass was produced  from inulinase hydrolysis stage by inulinase enzyme from Aspergillus clavatus-CBS5. Separation of inulinfiber aims to obtain inulin fiber through a microfiltration (MF) membrane 0.45 μm at room temperature, 400 rpm stirrer cycle and 40 psia for 0, 30, 60, 90 and 120 min. The results showed that best separation time was 120 minutes based on optimal CBC (cholesterol binding capacity) which fermented inulin fiber concentrate was produced with a total sugar concentration of 105.21 mg/mL, total solids 2.11%, total fiber 23.36%, total acid 6.66% (dry weight), 4.05 mg of dissolved protein/mL and CBC 13.781 mg/g. MF membrane increased the CBC by 23.4% compared to no separation process.Keywords: cholesterol binding capacity, inulin fiber, microfiltration membrane, permeate, retentateABSTRAKKondisi Sel Filtrasi Berpengaduk (SFB) digunakan sebagai acuan menuju kondisi proses skala besar (modul) terhadap pemisahan serat inulin terfermentasi oleh Lactobacillus acidophillus. Hidrolisat inulin yang digunakan sebagai biomassa dihasilkan dari tahapan hidrolisa inulin oleh enzim inulinase dari kapang Aspergillus clavatus-CBS5. Pemisahan serat inulin bertujuan untuk mendapatkan serat inulin melalui membran mikrofiltrasi (MF) 0,45 µm pada temperatur ruang, kecepatan putaran pengaduk 400 rpm dan tekanan 40 psia selama 0, 30, 60, 90 dan 120 menit. Hasil penelitian menunjukkan bahwa waktu pemisahan yang lama dapat menahan dan meningkatkan padatan total, serat total, asam total, protein terlarut dan kemampuan pengikat kolesterol (KPK) tetapi menurunkan gula total dalam retentat. Membran mikrofiltrasi melewatkan dan menurunkan gula total, protein terlarut dan KPK tetapi meningkatkan padatan total, serat total, asam total dalam permeat. Berdasarkan KPK optimal, waktu pemisahan terbaik dicapai setelah 120 menit. Pada kondisi ini dihasilkan konsentrat serat inulin terfermentasi dengan konsentrasi gula total 105,21 mg/mL, padatan total 2,11%, serat total 23,36%, asam total 6,66% (berat kering), protein terlarut 4,05 mg/mL dan KPK 13,781 mg/g. Membran MF mampu meningkatkan KPK 23,4% dibandingkan tanpa  menggunakan proses pemisahan.Kata kunci:          kemampuan pengikat kolesterol, membran mikrofiltrasi, permeat, retentat, serat inulin
Kajian Isolat Bakteri Asam Laktat dalam Menurunkan Kolesterol Secara In Vitro dengan Keberadaan Oligosakarida Maryati, Yati; Nuraida, Lilis; Hariyadi, Ratih Dewanti
Agritech Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.675 KB) | DOI: 10.22146/agritech.12865

Abstract

This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the performance of the LAB isolates in lowering cholesterol were also evaluated. The tests were done in MRS based medium in vitro with or without oligosaccharides i.e. galactooligosacharrides (GOS), fructooligosaccharides (FOS), inulin, hydrolyzed inulin or combination of oligosaccharides as prebiotics. The results revealed that all isolates were able to reduce cholesterol in the medium, and the highest cholesterol reduction was observed for L. acidophilus FNCC0051 and L. rhamnosus R23. There are two different mechanism in the loweringof cholesterol; cholesterol assimilation and cholesterol binding on the cell surface. For the case of P. pentosaceus 1-A38, it involves the assimilation, while the other four isolates may involve cholesterol binding on the cell surface. In addition, the tested LAB’s has different ability to use prebiotics, as shown by the reduction of total sugar in the medium. Oligosaccharides metabolism by L. acidophilus FNCC0051 and L. rhamnosus R23 resulted in several organic acid and SCFA with lactic acid produced as the largest proportion followed by acetic acid. Furthermore, the proportion of propionic and butyric acids were influenced by the type of isolates and carbon source. L. acidophilus FNCC 0051 was able to reduce cholesterol in the MRS based medium with oligosaccharides and their combination as carbon source and cholesterol reducing ability seems to involve both assimilation and cholesterol binding on the cell surface.ABSTRAKPenelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL) dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS), fruktooligosakarida (FOS), inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada permukaan sel.
POTENCY OF AMINO ACIDS AS SAVORY FRACTION FROM VEGETABLE BROTH OF MUNG BEANS (Phaseolus radiatus L.) THROUGH BRINE FERMENTATION BY Rhizopus-C1 Susilowati, Agustine; Aspiyanto, Aspiyanto; Moerniati, Sri; Maryati, Yati
Indonesian Journal of Chemistry Vol 9, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.343 KB) | DOI: 10.22146/ijc.21553

Abstract

Amino acids produced through brine fermentation of mung beans (Phaseolus radiatus sp) by inoculum of Rhizopus-C1 at room temperature for 0, 2, 4, 6, 8, 10, and 12 weeks, respectively had a potential use as savory fraction for seasoning agent. The objective of this experiment was to find out characteristic of produced amino acids and composition of fermentation products relating with proteolitic and amylolitic activities of Rhizopus-C1. The result of experiment showed that the length of fermentation time would increase intensity of savory taste and cloudy color, and increase total protein, soluble protein, and N-amino concentrations, decrease water, while fat concentration was constant. Fermentation of 10 weeks was optimal time to get crude broth with concentrations of total protein of 9.5622%, soluble protein of 8.5 mg/g, N-amino of 5.6 mg/g, fat of 0.2802%, water of 40.7189%, Volatile Reduction Substances (VRS) of 90 µeq/g, and reduction sugar of 672.5 mg/mL. Kinds of dominant non-essential amino acids produced were glutamic acid (1.014%), and aspartic acid (0.507 %), while essential amino acids were lysine (0.474%), and isoleucine (0.644%). The other of amino acids were resulted with concentration of 0.211 - 0.345%, such as leucine, arginine, serine, glycine, histidine, alanine, proline, tyrosine, valine, methionine, cystine, threonine, and phenilalanine. Visually, crude vegetable broth produced through brine fermentation of mung beans by Rhizopus sp-C1 was semi solid, brownish color, rather fatty, salty, and enough strong savory taste.   Keywords: Amino acids, brine fermentation, mung beans broth, Rhizopus-C1, savory fraction
Recovery of Fermented Spinach (Amaranthus sp.) Concentrate Through Ultrafiltration Membrane Process as Source of Folic Acid for Smart Food Formula Aspiyanto, Aspiyanto; Susilowati, Agustine; Lotulung, Puspa Dewi; Melanie, Hakiki; Maryati, Yati
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.993 KB) | DOI: 10.22146/ijc.25165

Abstract

Fermentation process on spinach (Amaranthus sp.) by Kombucha culture was done as an effort to recover naturally folic acid as bioactive components to increase smartness. The experimental activity was done by means of UF membrane (100,000 MWCO) fitted in Stirred Ultrafiltration Cell (SUFC) at stirrer rotation speed 200 and 400 rpm, room temperature, pressure 20 and 40 Psi for 30 min. Result of experimental activity showed that based on both selectivity and recovery of folic acid, process optimization of UF was reached at stirrer rotation speed 200 rpm and pressure 40 Psi. In the optimum condition, SUFC technique was able to recover folic acid in retentate 67.75% and in permeate 97.27% (63.19 µg/mL). Identification of monomer in permeate from the optimum process treatment was find out folic acid monomer with molecular weight (MW) 441.39 and relative intensity 93% at mass spectra T2.32 between m/z 257–304 and glutamic acids monomer with MW 148.57 and relative intensity 0.22% at mass spectra T2.82 between m/z 415–470. Other dominant monomer were folic acid fraction.
Green tea (Camellia assamica) concentrate as a source of L-theanine used in kombucha fermentation for relaxation drink1) Konsentrat teh hijau (Camellia assamica) sebagai sumber L-theanine yang digunakan pada fermentasi kombucha untuk minuman relaksasi SUSILOWATI, Agustine; ASPIYANTO, .; MELANIE, Hakiki; MARYATI, Yati
E-Journal Menara Perkebunan Vol 78, No 2: Desember 2010
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v78i2.67

Abstract

Abstrak Konsentrat teh hijau (Camellia assamica) grade Pekoe yang dihasilkan melalui pemurnian oleh membran mikro-filtrasi (MF) berpotensi sebagai substrat pada fermentasi  kombucha untuk efek relaksasi (anti stres) karena kandungan L-theanine yang tinggi. Kegiatan penelitian dilakukan untuk mendapatkan ratio terbaik antara konsentrat teh hijau dan air guna menghasilkan komposisi teh kombucha yang setara dengan teh kombucha komersial sebagai minuman santai. Fermentasi dilakukan pada nisbah konsentrat teh hijau dan air 1:4, 1:3, 1:2, 1:1 dan 1: 0 (v/v) dengan starter Kombucha komersial 0,5 % (b/v) pada suhu kamar selama satu minggu. Sebagai kontrol digunakan teh kombucha dengan nisbah teh hijau komersial dan air adalah 1:0. Hasil penelitian menunjukkan bahwa kandungan L-theanine dalam produk teh kombucha yang setara dengan produk komersial (2,51 % berat kering) dicapai pada nisbah konsentrat teh hijau dan air 1 : 2 (2,16 % berat kering) dan 1:1 (3,01 % berat kering). Selain  L-theanine, pada ratio konsentrat teh hijau dan air tersebut teh kombucha yang dihasilkan mengandung total polifenol 1,03 %, total padatan 11,67 %, total asam 0,53 % dan gula pereduksi 27,4 mg/mL, serta jumlah mikroba total log 5,88 CFU/mL atau 7,72 x  105 CFU/mL.Abstract Concentrate of green tea (Camellia assamica) of Pekoe grade produced through purification by means of microfiltration (MF) membrane is potentially used as a substrate in kombucha fermentation for relaxation (anti stress) due to high L-theanine concentration. This experiment was conducted to find out the best ratio of green tea concentrate and water in order to generate kombucha  tea composition   that  is  equal  to commercially available kombucha tea as relaxation drink. Fermentation   process   was    performed   with   green  tea concentrate and water ratio of 1: 4, 1: 3, 1: 2, 1: 1 and  1: 0 (v/v) using 0.5  % (w/v) of commercial kombucha starter at room temperature for one week. As a control kombucha tea  with ratio of commercial green tea and water of  1 : 0 was used. The result shows that L-theanine content in kombucha tea product equal to commercial kombucha tea (2.514  %, dry weight) was reached at the ratio of green tea concentrate and water of 1 : 2 (2.16  %, dry  weight)  and 1 : 1 (3.01 %, dry weight). Beside L-theanine, kombucha tea resulted from this ratio of green tea concentrate and water contains total polyphenol 1.03  %, total solids 11.67 %, total acids 0.53 % and reducing sugar 27.4 mg/mL, and total microbial counts of log 5.88 CFU/mL or 7.72 x 105 CFU/mL.
Formulasi Nikstamal Jagung, Tempe, dan Sayuran Terfermentasi dalam Perolehan Pasta Fortifikan sebagai Sumber Asam Folat Alami Susilowati, Agustine; Maryati, Yati; Lotulung, Puspa Dewi N.; Aspiyanto, Aspiyanto
Jurnal Aplikasi Teknologi Pangan Vol 7, No 2 (2018): Mei 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.805 KB) | DOI: 10.17728/jatp.2517

Abstract

Penelitian ini bertujuan untuk melakukan formulasi dalam pembuatan pasta fortifikan asam folat alami yang terdiri dari campuran tempe (kedelai dan kacang hijau), sayuran terfermentasi (brokoli dan bayam), dengan nikstamal jagung kuning dan jagung putih jenis gigi kuda guna mengetahui rasio terbaik campuran ketiga bahan tersebut terhadap konsentrasi asam folat dan identifikasi monomer-monomernya. Rangkaian proses fermentasi tempe dan sayuran masing-masing dilakukan menggunakan Rhizopus oligosporus strain C1 dan kultur Kombucha, serta nikstamalisasi jagung dilakukan dengan menggunakan Ca(OH)2. Formulasi dilakukan dengan mengatur rasio campuran tempe plus sayuran terfermentasi dan nikstamal jagung pada rasio 1:1, 1:2, 1:3, 1:4, dan 1:5. Analisis dilakukan terhadap protein terlarut, total padatan, gula reduksi, total gula dan asam folat. Identifikasi asam folat dan asam glutamat dilakukan melalui LC-MS. Penelitian ini berhasil untuk menentukan rasio terbaik guna menghasilkan asam folat tertinggi serta berhasil mengidentifikasi monomer asam folat dan asam glutamat pasta fortifikan asam folat. Kesimpulannya, formulasi pasta fortifikan asam folat terbaik dapat dicapai pada kombinasi pasta campuran tempe plus sayuran terfermentasi dengan nikstamal jagung pada rasio 1:5.Formulation Nixtamalized Corn, Tempeh, and Fermented Vegetables for Producing Fortificant Paste as Natural Follic Acid SourceAbstractThis research was done to obtain the highest concentration of folic acid and the monomer identification on paste formulation with various additions of natural folic acid source from tempeh (G. soyae and mung beans/P. radiatus), and fermented broccoli, and fermented spinach with both nixtamalized yellow corn and white corn (Zea mays L. var. indentata). The research was conducted through a series of tempe and vegetable fermentation process using Rhizopus oligosporus strain C1 and Kombucha culture, and nixtamalization of corn was done using Ca(OH)2. Ratio was set at 1:1, 1:2, 1:3, 1:4 and 1:5 of mixture paste of tempeh & fermented vegetables to nixtamalized corn, respectively. The result of experiment showed that nixtamalized corn ratio resulted in the increase in folic acid, total solids, and decreased dissolved protein, total sugars and reducing sugars. As conclusion, based on the best concentration of folic acid, optimization of formulation was achieved at combination between mixture of mung beans tempeh & fermented broccoli and a 1:5 ratio of corn.•|•|•|•|•|•