Articles

EFEK SITOTOKSIK EKSTRAK ETANOLIK HERBA SELEDRI (APIUM GRAVEOLENS L.) PADA SEL KANKER T47D, WIDR, DAN HELA Palupi, Kartika Dyah; Wulandari, Ainun; Goenadi, Fina Aryani; Nur, Kholid Alfan; Fitriasari, Aditya; Meiyanto, Edy
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 1, No 2 (2011): Oktober 2010 - Maret 2011
Publisher : University of Muhammadiyah Malang

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Abstract

Celery (Apium graveolens L.) is commonly used to lower blood pressure, antirheumatic, relaxant, mild diuretic, antiseptic for the urinary tract, antioxidant, and anti-inflammation. According to previous studies, a number of the phytochemicals found in the plant show cytotoxicity toward some types of cancer cells. However, studies on the cytotoxic effects of celery herb ethanolic extract (CEE) on breast cancer cell (T47D), colon cancer cell (WiDr), and cervix cancer cell (HeLa), however, has not been done yet. Our research aims at doing so. Cytotoxicity test was conducted using MTT assay and its absorbance was read using ELISA reader at ? = 595 nm. Results of the assay show that CEE reduces cell viability at concentrations of 100-750 µg/ml on HeLa cells, while reduction of T47D and WiDr cell viability was not achieved until concentrations of 500-750 µg/ml. Based on these results, we conclude that CEE hold many potentials for further developments as preventive and therapeutive agent in cancer treatment.
EFEK ANTI PROLIFERATIF EKSTRAK ETANOL KULIT BATANG TANAMAN CANGKRING (ERYTHRINA FUSCA LOUR) TERHADAP SEL MYELOMA Meiyanto, Edy; Sismindari, Sismindari; Triastuti, Asih
Jurnal Ilmiah Farmasi Vol 1, No 1 (2004)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTErythrina fusca Lour has been traditionally used to cure hepatosis, malaria, hematuria, andcancer. The bark of this plant contains  carotene, polifenol, thiamin, saponin, and alkaloiderythralin and erythramin. The aim of this research was to know the underlying mechanism of itseffect as antiproliferative against Myeloma cells. The bark powder was extracted using ethanol(70%) and was used for the experiment after freezed drying. Citotoxicity test of this extractperformed LC50 of 0,367 mg/ml. The rate of proliferation was observed by doubling time effectagainst proliferating cells. The cells were exposed with ethanolic extract in RPMI 1640 mediumcontaining 1) 0,25 mg/ml 2) 6,25x10-2mg/ml, and 3) 1,56x10-2mg/ml and every 0, 6, 12, 24, 48,and 72 hours cell were counted. The result showed that extract treated cells delayed proliferation atall concentration with doubling time dose 2) of 161, 38 hours, and dose 3) of 93,91 hours, whereasdoubling time of control cells were 69,86 hours. Ethidium bromide staining of extract treated cellsshowed apoptosis like profile. These results indicated that ethanolic extract of the bark of Erythrinafusca Lour has an antiproliverative effect on Myeloma cell line. Several mechanisms might accountfor this effect, like inhibiting cell cycle progression, signal transduction, causing delayed andapoptosisKeywords: Erythrina fusca Lour, atiproliferative, Myeloma
Structure Modification of Ethyl p-methoxycinnamate Isolated from Kaempferia galanga Linn. and Citotoxicity Assay of The Products on WiDr Cells Ekowati, Juni; Rudyanto, Marcellino; Sasaki, Shigeru; Budiati, Tutuk; Sukardiman, .; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Ethyl p-methoxycinnamate, major ingredient of Kaempferia galanga rhizome, have been reported not only has analgesic – anti inflammatory activities like NSAIDs which inhibited cyclooxygenase, but also inhibit tumor cell proliferation in specimen of mouse epidermis. Therefore, it will be interesting to carry out  synthetic studies on the derivates of ethyl  p-methoxycinnamate and searching their citotoxic activity on WiDr cell. We wish to report of structure modification on carboxyl moiety of  ethyl p-methoxycinnamate  and  evaluation on their citotoxic activity  on WiDr cell. Isolation of ethyl p-methoxycinnamate from Kaempferia galanga rhizome was carried out by percolation with ethanol 96% as solvent. Hydrolysis of ethyl p-methoxycinnamate in basic condition was performed to obtain p-methoxycinnamic acid. Preparation of some thiourea derivates of ethyl  p-methoxycinnamate was carried out  by microwave irradiation. Citotoxicity assay was carried out by MTT method for 48 h.   Modification  of  carboxyl  group  of  ethyl  p-methoxycinnamate to its thiourea form could be carried out by microwave irradiation gave; (E)-3-(4-methoxyphenyl)-N-(phenylcarba- mothioyl)acrylamide (50%); (E)-3-(4-methoxyphenyl)-N-(4-methoxyphenylcarbamothi- oyl)acrylamide (26%) and (E)-3-(4-methoxyphenyl)-N-(4-methylphenylcarbamothioyl) acrylamide (54%), yield calculated for 2 step from the acid chloride. All compounds showed no citotoxic effect on WiDr cell at 48 h incubation.
SynergisticCombinationofCiplukan(Physalis angulata) HerbsEthanolicExtractandDoxorubicinonT47DBreast CancerCells Armandari, Inna; Palupi, Kartika Dyah; Farida, Sofa; Hermawan, Adam; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Doxorubicinisoneofchemotherapeuticagentwidelyusedinbreastcancertreatment,but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs such as ciplukan herb. This research was conducted to explorecytotoxicactivityofsingleciplukanherbsethanolicextractanditscombinationwith doxorubicinonT47Dbreastcancercells.Cytotoxicactivityofciplukanherbsethanolicextract only and its combination with doxorubicinwere tested on T47D cells using MTT assay toobtainIC50valueandcombinationindex(CI),respectively.Singleextractshowedcytotoxic activityonT47DcellswithIC50valueofwas160*g/ml.Thus,combinationtreatmentfrom ciplukanherbsethanolicextractanddoxorubicinshowedsynergisticeffect(CI<1,0).Thiseffect wasreachedatconcentrationofciplukanherbsethanolicextract-doxorubicin80μg/ml-2nM, 80 μg/ml-4 nM, and 80 μg/ml-8 nM. This research indicated that ciplukan herbs ethanolic extractispotentialtobeappliedasco-chemotherapeuticagentinbreastcancertherapy.
Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells Istiaji, Raditya Prima; Fitria, Maya; Larasati, .; Tjondro, Fortunella; Maruti, Astrid Ayu; Setyowati, Erna Prawita; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value).  The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed  the effect of synergism combination. The Ooptimum concentration of combination was  1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored.
Combination of Solanum nigrum L. Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cells Anindyajati, .; Sarmoko, .; Putri, Dyaningtyas D. P.; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Leunca (Solanum nigrum L.) has been proven to possess  anticancer activity on some type of cancer cells. In vitro study of solamargine found in the herb showed cytotoxic effect against several breast cancer cell lines, such as T47D and MDA-MB-31. Hence, further study on its potential as a co-chemotherapeutic agent needs to be conducted, in order to overcome resistance problem commonly found in cancer  chemotherapy. This study aimed to examine the cytotoxic activity of leunca herb ethanolic extract (LEE) alone and its combination with doxorubicin. Single and combinational treatment of LEE and doxorubicin on T47D breast cancer cells were done, and their viability representing cytotoxicity were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinational effect.  Twenty four hours-treatment of LEE  alone gave cytotoxicity activity showing a dose-dependent manner with the IC50 of 47 µg/ml, while combinational treatment showed that 4 µg/ml LEE was found to be synergist with 4 nM doxorubicin on T47D cells, with the optimum CI value of 0.59. This result shows that Solanum nigrum L. is potential to be proposed as doxorubicin co-chemotherapeutic agent against breast cancer. Further study on its molecular mechanism needs to be conducted.
Ethanolic Extract of Moringa oleifera L. Increases Sensitivity of WiDr Colon Cancer Cell Line Towards 5-Fluorouracil Nur, Kholid Alfan; Putri, Herwandhani; Cahyani, Fany Mutia; Katarina, Aulia; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

For more than four decades, combination chemotherapy (co-chemotherapy) has been employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our research is to investigate the activity of  Moringa oleifera  L. (tanaman kelor) ethanolic extract (MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line. Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic activity based on percent cell viability via MTT  assay, and based on apoptosis observation via the double staining method using acrydin orange – ethidium bromide (AE) as the staining reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125, and 250 µg/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 µg/ml of MEE was chosen as the combination concentrations with 1000 µM 5-FU. MTT assay 24 hours and 48 hours post-combination treatment showed significant cell viability reduction in comparison to those of single treatments. Apoptosis observation using the double staining method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a potential co-chemotherapy agent  by increasing the sensitivity  of WiDr colon cancer cell line towards 5-FU.
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Kasianningsih, Sri; Rivanti, Erlina; Pratama, Ratih Hardika; Pratama, Nanda Resa; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim  for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley  rat that induced  by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats : control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed  by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of  doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells  T cells compared  to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has  immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.
Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression Pratama, Nanda Resa; Gilang, Yurista; Riata, Rita; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Hormone Replacement Therapy (HRT) is therapy for estrogen deficiency and post menopausal  syndromes,  but  high  cost  and  unwell-secured  therapy.  One  of  alternative therapy  is  the  usage  of  phytoestrogens.  The  banana  peel  contains  flavones,  flavonol, flavanone and polimethoxyflavone which are potential as phytoestrogen. The purpose of this study was to examine the estrogenic effect of banana peel extract (BPE) development of mammary gland of ovariectomized rats. Estrogenic effects was examined based on in vivo and in silico experiment. For in vivo experiment, female Sprague-dawley rats aged 50 days were ovariectomized. At 70 days of age, 12 rats were treated with BPE 500 mg/kgBB and 1000mg/kgBB, 5 rats were treated with estradiol 2g/day while others served as control were treated with CMC-Na 0.5% and sacrificed 2 weeks later. The base line ovariectomized rats and base line non-ovariectomized rats were sacrificed at 70 days of age. The in silico experiment examined by molecular docking between myricetin and estrogen receptor alpha (ER-α). The result of in vivo experiment showed that 1000 mg/kgBW BPE induced c-Myc expression  and  enhance  ovariectomized  rat  mammary  gland  development  significantly. Meanwhile, molecular docking showed that there are hydrogen bond interaction between bioactive compound in BPE and Estrogen Receptor (ER)-α but less powerfull than estrogen and ER-α interaction. In summary, BPE can act as an estrogen agonist,  resulting in the enhancement of c-Myc expression. 
Antiproliferative Activity of Ethanolic Extract of Ciplukan Herbs (Physalis angulata L.) on 7,12-Dimethylbenz[a]nthracene-Induced Rat Mammary Carcinogenesis Monikawati, Ameilinda; Farida, Sofa; Putri, Laras Widawaty; Ikhtiarsyah, Yurista Gilang; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Physalis angulata  L.  is  an  annual  herb  widely  used  as  popular  medicine  for  the treatment of cancer. Physalis angulata L. ethanolic extract (PEE) has been demonstrated to have strong cytotoxic activity against breast cancer, inhibited cancer cell’s proliferation and induced  cell  cycle  arrest.  The  aim  of  our  study  is  to  investigate  the  effect  of  PEE  as  a cancer  chemopreventive  agent  on  7,12-dimethylbenz[a]nthracene  (DMBA)-induced  rats mammary. The antiproliferative activity was characterized by monitoring the histopatology representation  and  expression  of  cell  proliferation  on  DMBA-induced  mammary  rats  that were  treated  with  PEE  against  control  groups.  The  histopatology  representation  were analyzed  by  Haematoksilin  Eosin  (HE)  staining  method,  while  proliferative  activity  was detected by AgNOR method. The HE staining results showed significant differences in cells morphology  of  treatment  groups  compared  to  the  control  groups.  Thus  results  suggest that  PEE  was  able  to  repair  morphology  of  cells  undergoing  carcinogenesis.  AgNOR method  showed  decreasing  occurrence  of  black  dots  between  treatment  and  control groups. Thus, we conclude that PEE has an antiproliferative activity on DMBA-induced rat mammary.  Therefore,  the  ethanolic  extract  of  Physalis  angulata  herbs  is  a  potential chemopreventive agent on cancer. Further study on its molecular mechanism needs to be explored. Keywords:  Physalis  angulata,  breast  cancer, 7,12-dimethylbenz[a]nthracene, carcinogenesis, antiproliferative
Co-Authors -, Sukardiman . Anindyajati . Larasati . Sarmoko . Sugiyanto . Sukardiman Adam Hermawan Aditya Fitriasari Afkari, Hanif Agung Endro Nugroho Agusta Fauzi, Ilham Ahmad Fudholi Ahsani, Anisa Fauzia Ainun Wulandari, Ainun Alan Anderson Bangun Alexxander, . Alexxander, . Amalina, Nur Dina Ameilinda Monikawati Andita Pra Darma Angraini, Sonia Meta ANINDYAJATI, ANINDYAJATI Anjarsari, Etyk Yunita Annisa Novarina Aprianto, Yoce Arief Nurrochmad Arief Rahman Hakim Asih Triastuti, Asih Asmah Susidarti, Ratna Astrid Ayu Maruti Aulia Katarina Ayu Maruti, Astrid B. Sudarto Bagaswoto Poedjomartono, Bagaswoto Barinta Widaryanti Beni Lestari, Beni Broto Kardono Chandra Risdian D., Andita Pra D., Andita Pra DA'I, MUHAMMAD Daâ??i, Muhammad Da’i, Muhammad Devi Nisa Hidayati Dewi Arum Sekti, Dewi Arum Dewi Pamungkas Putri, Dyaningtyas Dewi Pratiwi Dilalah, Idlohatud Dita Brenna Septhea Djaswadi Dasuki Dwi Ana Nawangsari, Dwi Ana Dwi Nurahmanto, Dwi Dyaningtyas D. P. Putri Dyaningtyas Dewi Pamungkas P, Dyaningtyas Dewi Dyaningtyas Dewi Pamungkas Putri Dyaningtyas Dewi Putri, Dyaningtyas Dewi Ediati Sasmito Endah Puji Septisetyani, Endah Puji Endah Puspitasari Endang Purwaningsih Erlina Rivanti Erna Prawita Setyowati Ertanto, Yogi Fadliyah, Hilyatul Fany Mutia Cahyani Farmasyanti, Cendrawasih Andusyana Feby Handoko, Fransiscus Fikri Amalia Fina Aryani Goenadi, Fina Aryani Fitria Rahmi FITRIASARI, ADTYA Fiveri, Anis Fiveri, Anis Fortunella Tjondro Hadi, Ismanurrahman Hairunisa, Indah Hanif, Naufa Hendra K. Maury, Hendra K. HENDRI WASITO Heni Susilowati Herwandhani Putri Husnaa, Ulfatul Ibrahim Arifin Ika Nurzijah Ika Rahmawati Sutejo Ikawati, Muthi' Ilham Agusta Fauzi Ilmawati, Gagas Pradani Nur Imono Argo Donatus, Imono Argo Indri Kusharyanti Inna Armandani, Inna Inna Armandari Istighfari Jenie, Riris Iwan Sahrial Hamid JAKA WIDADA Juni Ekowati Kadarsih Soejono, Sri Kadarsih Soejono, Sri Kartika Dyah Palupi Kholid Alfan Nur Kristina, Nita Kuijpers-Jagtman, Anne Marie Lany Candra, Lany Laras Widawaty Putri Larasati Larasati Larasati, Yonika Arum Luthfia Indriyani M, Kawaichi M, Kawaichi Mae Sri Hartati Wahyuningsih, Mae Sri Hartati Marcellino Rudyanto Maria Dwi Supriyati, Maria Dwi Marissa Angelina Masashi Kawaichi, Masashi Matsuda, Eishou Maya Fitria Moordiani ., Moordiani Muflikhasari, Haruma Anggraini Muhammad Da’i, Muhammad Muhammad Fithrul Mubarok, Muhammad Fithrul Muthi Ikawati Muthi’ Ikawati N., Perdana Adhi N., Perdana Adhi Nanda Resa Pratama Ni Putu Linda Laksmiani Niken Nur W, Niken Novi Hastuti, Novi Novitasari, Dhania Nugraheni, Nadzifa Nunuk Aries Nurulita Nurhayati, Ika Putri Nurma Sabila Nurrachma, Marsya Yonna P.K.W., Diah Ayu P.K.W., Diah Ayu Perdana Adhi Nugroho Prisnu Tirtanirmala Pudjono Pudjono Putri, Asri Mega Putri, Nindya Budiana Qodria, Lailatul R A Susidarti Rachmady, Rahmawaty Rachmaniar Rahmat, Rachmaniar Raditya Prima Istiaji Rahmi Khamsita Raisatun Nisa Sugiyanto Ramadani, Ratna Dwi Ratih Hardika Pratama Ratna Asmah Susidarti Retno Arianingrum Retno Murwanti Retno Murwantib Retno Sunarminingsih, Retno Rina Andriyani Riris I Jenie, Riris I Riris Istighfari Jenie Rita Riata Rohmad Yudi Utomo Rosa Adelina Rosana Anna Ashari, Rosana Anna Rosita Melannisa, Rosita Rosye H.R. Tanjung Rul Afiyah Syarif, Rul Afiyah Santoso, Ragil Anang Saputri, Dian Sari Haryanti Sari, Nur Fitra Sarmoko Sarmoko Sendy Junedi, Sendy Sendy Junedy, Sendy Septriyanto Dirgantara Shigeru Sasaki Shirly Kumala Sismindari Sismindari Sismindari, ., Sismindari, Sitarina Widyarini Sofa Farida Sofia Mubarika Sri Handayani Sri Kadarsih Soejono Sri Kasianningsih Sri Susilowati Sri Tasminatun, Sri Sudarsono . Sugeng Riyanto Sugiyanto . Sugiyanto Sugiyanto Supardjan A. M., Supardjan A. Supardjan A.M., Supardjan Supardjan AM, Supardjan Susi Ari Kristina Suven ., Suven SUWIJIYO PRAMONO Tutuk Budiati Umar A. Jenie Umar Anggara Jenie Yohanes Sardjono, Yohanes Yurista Gilang Yurista Gilang Ikhtiarsyah Yuyun Farida, Yuyun Zalinar Udin Ziana Walidah, Ziana