I. Mokoginta
Bogor Agricultural University, Department of Aquaculture

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THE IMPROVEMENT OF PROTEIN RETENTION THROUGH A HIGHER UTILIZATION OF CARBOHYDRATE IN THE DIET WITH CHROMIUM FOR COMMON CARP CYPRINUS CARPIO LINN. Mokoginta, I.; Hapsyari, F.; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.7 KB) | DOI: 10.19027/jai.3.37-41

Abstract

This experiment was conducted to improve the protein retention through carbohydrate efficiency in the chromium diet of common carp Cyprinus carpio. Four experimental diets with isonitrogen and isocaloric but different Cr+3 level 0,00; 1,33; 2,23 and 3,96 ppm were used in this experiment. Fish with body weight of 5,31 ± 0,02 gram were reared in aquaria (50 x 40 x 35 cm); 20 fish per aquarium. Fish fed upon the experimental diets three times daily, at satiation for 50 days. Results of this experiment showed that the protein retention could be improved by given Cr+3 in the diet. Based on the respon curve of protein retention (Yi = -1.1678X2 + 3,7223X + 24,818 ; R2 = 0,9) it was found that the optimum Cr*J in the diet was 1,59 ppm. The maximum relative growth rate was found at 2,16 ppm Cr3+ (Y2 = -14,592 X: + 63,18IX + 426,43 ; R = 0,9). The feed efficiency and the survival rate of the fish are the same between treatment (p>0.05). The protein level of the fish from treatment 3,96 ppm Cr+3 was lower than the others; the lipid level of Cr+3 diets was higher than that of non Cr+3 diet; however the carbohydrate level of fish from diet 2,23 Cr+3 was lower than the others. It was concluded that the optimum level of Cr +3 in the diet of common carp was 1,59-2,16 ppm Key words : dietary chromium, common carp, Cyprinus carpio   ABSTRAK Penelitian ini bertujuan untuk meningkatkan retensi protein dan pertumbuhan ikan mas Cyprinus carpio melalui peningkatan efisiensi karbohidrat pakan yang diberi kromium (Cr"3). Empat macam pakan dengan kadar Cr+3 yang berbeda yaitu 0,00; 1,33; 2,23 dan 3,96 ppm tetapi kadar protein dan energi yang sama digunakan pada penelitian ini. Ikan mas berbobot 5,31 ± 0,02 gram per ekor dipelihara dalam akuarium (50x40x35 cm) dengan kepadatan 20 ekor. Ikan diberi pakan tiga kali sehari secara at satiation. Pemberian pakan dilakukan selama 50 hari. Hasil penelitian memperlihatkan bahwa adanya pemberian Cr+3 dalam pakan akan mengefisienkan penggunaan karbohidrat pakan sehingga retensi protein akan meningkat pula. Retensi protein tertinggi dicapai pada kadar Cr+3 1,59 ppm (dihitung dari persamaan y, = -1,1678X2 + 3,7223X + 24,818; R2 = 0,9). Pertumbuhan relatif tertinggi dicapai pada kadar Cr+3 2,16 ppm (dihitung dari y2 = -14,592 X2 + 63.181X + 426,43; R2 = 0,9); sedangkan efiseinsi pakan dan tingkat kelangsungan hidup antar perlakuan sama (p>0,05). Kadar protein tubuh perlakuan 3,96 ppm Cr+3 relatif lebih rendah dari perlakuan lainnya. Kadar lemak tubuh ikan yang diberi Cr+3 lebih tinggi dari yang tidak diberi Cr+3; sedangkan kadar karbohidrat tubuh terendah pada perlakuan 2,23 ppm Cr+3. Secara keseluruhan dapat disimpulkan bahwa kadar Cr+3 optimum dalam pakan ikan adalah 1,59-2,16 ppm. Kata kunci : Kromium pakan. ikan mas, Cyprinus carpio
THE EFFECT OF ENRICHED DAPHNIA SP. WITH DIFFERENT SOURCE OF OIL ON THE SURVIVAL RATE AND THE GROWTH OF OREOCHROMIS NILOTICUS LARVAE Mokoginta, I.; Jusadi, Dedi; Pelawi, T.L.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.769 KB) | DOI: 10.19027/jai.2.7-11

Abstract

ABSTRACTThis experiment was conducted to evaluate best source of oil to enriched Daphnia sp. before fed it to Oreochromis niloticus larvae. Four treatments were used in this experiment; first, Daphnia sp. without encrichment, second Daphnia sp. enriched with fish oil, third Daphnia sp. enriched with corn oil and the fourth Daphnia sp. enriched with coconut oil. Three days old larvae fed on Daphnia sp. with size of £ 0,5 mm at the first week and 0,6 ? 1,0 mm as the second week of this experiment. Larvae fed on Daphnia sp. 5 times daily, ad libitum, for 14 days. Larvae was reared in the small cages (2,25 l), and all cages was placed in the aquarium. Larvae density was 48 larvae/l. This experiment showed that the lipid level in Daphnia sp. enriched with oil was higher than that of no enrichment Daphnia sp. The highest n3- fatty acid level was found in Daphnia sp. enriched with fish oil, and the highest n6- fatty acid level was found in Daphnia sp. enriched with corn oil. Larvae fed on Daphnia sp. enriched with oil have a higher relative growth rate than that fed on Daphnia sp. without enrichment. The highest survival rate of larvae was found by feeding them with Daphnia sp. enriched with corn oil (p < 0,05).Key words : Daphnia sp., enrichment, larvae, Oreochromis niloticus ABSTRAKPenelitian ini dilakukan untuk mengevaluasi sumber minyak yang terbaik bagi pengkayaan Daphnia sp. sebelum diberikan ke larva ikan nila, Oreochromis niloticus. Ada 4 perlakuan dalam penelitian ini yaitu Daphnia sp. yang tidak diperkaya minyak; Daphnia sp. yang diperkaya minyak ikan; Daphnia sp. yang diperkaya minyak jagung; dan Daphnia sp. yang diperkaya minyak kelapa. Larva ikan nila, Oreochromis niloticus berumur 3 hari ditebar dalam hapa kecil (volume 2,25 l) sebanyak 48 ekor/l, dan seluruh hapa diletakkan dalam akuarium berukuran 100x50x40 cm. Daphnia sp. diperkaya terlebih dahulu dengan minyak sesuai perlakuan sebelum diberikan ke larva. Pada minggu pertama pemeliharaan larva, ukuran Daphnia sp. yang digunakan adalah £ 0,5 mm dan pada minggu ke dua 0.6 ? 1.0 mm. Daphnia sp. diberikan sebanyak 5 kali dalam sehari secara ad libitum dan pemberian pakan dilakukan selama 14 hari. Hasil penelitian ini memperlihatkan bahwa kadar lemak Daphnia sp. yang diperkaya minyak lebih tinggi dari Daphnia sp. yang tidak diperkaya, dan hal ini berpengaruh pula pada kadar lemak tubuh larva. Kadar asam lemak ?n3 tertinggi terdapat pada Daphnia sp. yang diperkaya minyak ikan dan kadar asam lemak ?n6 tertinggi terdapat pada Daphnia sp. yang diperkaya minyak jagung. Larva yang diberi Daphnia sp. yang diperkaya dengan minyak mempunyai pertambahan bobot relatif dan tingkat kelangsungan hidup yang lebih tinggi dari larva yang diberi Daphnia sp. yang tidak diperkaya; dan tingkat kelangsungan hidup larva yang tertinggi dihasilkan oleh perlakuan pemberian Daphnia sp. yang diperkaya minyak jagung (P < 0,05), walaupun pertumbuhan bobot relatifnya sama dengan perlakuan lainnya (p > 0,05).Kata kunci : Daphnia sp., pengkayaan, larva, Oreochromis niloticus
THE EFFECT OF DIETARY VITAMIN E (-TOCOPHEROL) LEVEL ON THE LIPID, ESSENSIAL FATTY ACID LEVEL AND THE HATCHING RATE OF CATFISH, CLARIAS BATRACHUS LINN. Mokoginta, I.; Syahrizal, ,; Jr., M. Zairin
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.231 KB) | DOI: 10.19027/jai.1.75-80

Abstract

This experiment was conducted to evaluate the dietary level of vitamin E (a-tocopherol) on the lipid, essential fatty acid level and the hatching rate of catfish, Clarias batrachus Linn. Five experimental diets with isoenergy and isonitrogen, but different vitamin E (a-tocopherol) level were used in this experiment. Dietary vitamin E levels are 18, 114, 210, 308 and 416 mg/kg diet respectively.  Fish with body weight of 18,55 a 5,11 g per fish, fed on the experimental diets at satiation, for six months.  The vitamin E, the lipid and the n-6 and n-3 fatty acid level of egg increase as the dietary vitamin E level of broodstock diet increase. However, the ratio of n-6/n-3 fatty acid of egg produced  by treatment 210 mg vitamin E/kg diet was higher than that of other treatments, which finally produced the highest hatching rate of egg (p<0,05).Key words : Vitamin E (a-tocopherol), reproduction, catfish (Clarias batrachus Linn.)
BLOOD MEAL UTILIZATION AS ORGANIC FE SOURCE FOR POLKA DOT GROUPER CROMILEPTES ALTIVELIS GROWTH PERFORMANCE Setiawati, Mia; Purnama, P.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.395 KB) | DOI: 10.19027/jai.8.163-168

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This experiment was designed to examine the use of spray dried cell blood meal as organic Fe source in polka dot grouper (Cromileptes altivelis) feed to substitute inorganic Ferosulphate (FeSO4.7H2O). Two treatments were applied, one with FeSO4.7H2O as Fe source and the other with 6% blood meal. Fish with initial length and weight of 6.48 ± 0.16 cm and 5.60±0.25 g was reared at a density of 10 fish/aquarium (40 x 60 x 50 cm3) in a recirculation system for 40 days. Feed was administrated 3 times a day at satiation. The use of organic Fe with 6% blood meal apparently resulted in a lower body Fe content, feed consumption and lipid retention but a higher protein retention and feed efficiency. There was however no significant difference between treatments in growth and survival (P>0.05).   Keywords : Spray dried cell, polka dot grouper, growth, Fe, blood meal   ABSTRAK Penelitian dirancang untuk melihat pemanfaatan tepung darah spray-dried cell (SBC) sebagai sumber zat besi organik dalam pakan kerapu bebek (Cromileptes altivelis), menggantikan bahan anorganik Ferosulfat (FeSO4.7H2O). Penelitian menggunakan 2 jenis perlakuan yaitu pakan Fe-anorganik dengan sumber Fe dari FeSO4.7H2O dan sumber Fe-organik dari tepung darah 6 %. Panjang awal ikan 6,48±0,16 cm dan bobot awal 5,60±0,25 gr/ekor, kepadatan 10 ekor/akuarium (40x60x50cm3) dalam sistem resirkulasi. Pemeliharaan ikan selama 40 hari dengan pemberian pakan 3 kali sehari  sekenyangnya (at satiation). Parameter uji meliputi kinerja pertumbuhan dan komposisi tubuh ikan. Hasil penelitian menunjukkan penggunaan Fe-organik dengan 6 % tepung darah sebagai pengganti sumber Fe-anorganik pada kerapu bebek memberikan pengaruh terhadap penurunan kandungan Fe tubuh, konsumsi pakan dan retensi lemak, tetapi laju pertumbuhan harian, panjang relatif dan kelangsungan hidup sama (P>0,05). Penggunaan Fe-organik memberikan pengaruh terhadap peningkatan retensi protein dan efisiensi pakan. Kata kunci : spray-dried cell, kerapu bebek, pertumbuhan, Fe, tepung darah
OVARIAN DEVELOPMENT OF FEMALE MUD CRAB, SCYLLA SERRATA SUPPLEMENTED WITH CHOLESTEROL AND INJECTED WITH SEROTONIN Pattiasina, Betsy J.; Junior, M. Zairin; Mokoginta, I.; Affandi, R.; Manalu, W.
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.38 KB) | DOI: 10.19027/jai.9.67-76

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Cholesterol is known to play an important role in nutrition of crustacean and function as a precursor for steroids synthesis, while neurohormone of serotonin could induce ovarian maturation in crustacean. Ovarian development of adult females Scylla serrata was induced by adding cholesterol in the diet and serotonin injection. This research was designed to study the effectiveness of cholesterol supplementation and serotonin injection in ovarian development. Broodstocks were stocked in nine experimental units in three fiber tanks. The fiber tank was equipped with sands substrate and flow through seawater system. The experimental crabs were assigned into a completely randomized design with a 3 x 3 factorial arrangement. The first factor was cholesterol supplementation in the diet with 3 levels (0, 0,5 and 1,0%). The second factor was serotonin injection with 3 levels (0, 5 and 10 ?g/g BW). Samples of broodstock were taken every four days to evaluate the stages of ovarian maturity and parameters were used to evaluate the ovarian maturation stage are gonad index (GI) and oocyte diameter, concentration of estradiol 17?, yolk protein concentrations, and fecundity. Results showed that female crabs supplemented with 0,5% cholesterol and a combination of cholesterol 0,5% supplementation and injection serotonin with a dose of 10 ?g/g BW had better reproduction development. It is concluded that ovarian development of Scylla serrata could be improved by cholesterol supplementation and serotonin injection. Key words: Cholesterol, serotonin, ovarian development, Scylla serrata   ABSTRAK Kolesterol diketahui merupakan nutrien spesifik yang berperan dalam sisntesis hormon steroid dan mengontrol reproduksi, sementara serotonin merupakan salah satu neurohormon yang dilaporkan dapat merangsang pematangan ovari dan pemijahan pada krustase. Penelitian ini bertujuan untuk mengetahui pengaruh tingkat pemberian kolesterol yang optimal dalam pakan buatan, serta dosis penyuntikan serotonin yang efektif untuk mempercepat proses perkembangan dan pematangan ovarium induk kepiting bakau Scylla serrata. Pemeliharaan induk dilakukan dengan menggunakan tiga buah bak fiber. Bak pemeliharaan dilengkapi dengan substrat pasir dan sistim air laut mengalir. Eksperimen menggunakan rancangan acak lengkap (RAL) pola faktorial, dengan 9 satuan percobaan. Faktor pertama, suplemen kolesterol didalam pakan dengan 3 tingkat dosis (0; 0,5; dan 1%) dan faktor kedua, injeksi serotonin dengan 3 tingkat dosis (0, 5, dan 10 ?g/g bobot tubuh). Pengamatan terhadap tingkat kematangan ovari dilakukan setiap 4 hari sekali. Paramater pengambilan sampel meliputi  tingkat kematangan ovari, indeks gonad dan diameter oosit, konsentrasi estradiol 17?, konsentrasi protein yolk, dan fekunditas. Hasil penelitian menunjukkan bahwa induk kepiting yang disuplementasi dengan dosis kolesterol 0,5% dan induk kepiting yang mendapat perlakuan kombinasi, suplementasi kolesterol 0,5% dan injeksi serotonin dosis 10 ?g/g bobot tubuh dapat menghasilkan perkembangan ovari yang terbaik. Jadi kolesterol dan serotonin dapat digunakan untuk meningkatkan perkembangan ovari. Kata-kata kunci: Kolesterol, serotonin, perkembangan ovari, Scylla serrata
REQUIREMENT OF N-6 AND N-3 FATTY ACID IN BROODSTOCK DIETS ON REPRODUCTIVE PERFORMANCE OF GREEN CATFISH, HEMIBAGRUS NEMURUS BLKR. Utiah, Adharto; Junior, M. Zairin; Mokoginta, I.; Affandi, R.; Sumantadinata, K.
Jurnal Akuakultur Indonesia Vol. 6 No. 1 (2007): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.829 KB) | DOI: 10.19027/jai.6.7-15

Abstract

This experiment was conducted to determine the optimum dietary level of n-6 and n-3 fatty acids on reproduction performance of green catfish. Four experimental diets with different level of n-6 and n-3 fatty acid (diet A: 0.77% n-6 fatty acid, 0.07% n-3 fatty acid; diet B: 1.56%,0.10 %; diet C: 1.74%, 0.13 % and diet D: 2%, 0.28%) were used in this experiment during seven month. Diets given twice every day in the morning and evening. The 28 broodstock used in this research with density every waring seven broodstock. Samples of eggs were analyzed for fatty acid composition. The diameter of eggs, fecundity, hatching rate of the eggs, survival rate and percentage of abnormal larvae were determined. Results showed that different dietary level of n-6 and n-3 fatty acids of the broodstock significantly affect the reproductive performance especially fecundity and hatching rate of eggs. The maximum fecundity, egg diameter, and hatching rate were produced broodstock fed on 1.56% n-6 fatty acid and 0.10 % n-3 fatty acid in diet by at the level of 12.29% lipid. Keywords : n-6 and n-3 fatty acids, reproduction performance, green catfish, Hemibagrus nemurus.   Abstrak Penelitian ini dilakukan pada berbagai level asam lemak n-6 dan n-3 dalam pakan untuk melihat pengaruhnya terhadap penampilan reproduksi dari ikan baung. Penelitian dilaksanakan selama 7 bulan.  Empat jenis pakan  digunakan dalam percobaan ini dengan perbedaan kandungan asam lemak n-6 dan n-3 (pakan A: 0,77% asam lemak n-6, 0,56% asam lemak n-3; pakan B: 1,56%, 0,78%; pakan C: 1,74%, 1,00% and pakan D: 2,03%, 1,82%). Dalam penelitian ini 28 ekor induk digunakan dan ditebarkan kedalam waring dengan kepadatan 7 ekor tiap waring. Pakan diberikan pagi dan sore hari secara at satiation.  Sampel telur dan larva dianalisa komposisi asam lemak. Penampilan reproduksi yang diamati adalah diameter telur, fekunditas, derajat penetasan telur, derajat kelangsungan hidup larva dan persentase larva abnormal. Hasil penilitian menunjukan bahwa perbedaan kandungan asam lemak n-6 dan n-3 dalam pakan berpengaruh pada komposisi asam lemak n-6 dan n-3 telur dan juga memberikan pengaruh yang berbeda nyata terhadap penampilan reproduksinya khusus pada fekunditas dan derajat penetasan telur. Lama waktu matang yang dicapai oleh tiap induk relatif sama antar perlakuan.  Fekunditas, diameter telur, derajat penetasan telur dan derajat kelangsungan hidup larva tertinggi diperoleh pada induk yang memperoleh pakan yang mengandung 1,56% asam lemak n-6 dan 0,78% asam lemak n-3. Kata kunci:  Asam lemak n-6 and n-3, penampilan reproduksi, ikan baung, Hemibagrus nemurus.
DIETARY ZINC REQUIREMENT OF YOUNG GIANT GOURAMY (OSPHRONEMUS GOURAMY, LAC.) Setiawati, Mia; Azwar, N.R.; Mokoginta, I.; Affandi, R.
Jurnal Akuakultur Indonesia Vol. 6 No. 2 (2007): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.885 KB) | DOI: 10.19027/jai.6.161-169

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This experiment was conducted to determine the dietary zinc requirement of young giant gouramy. Five experimental diets were used in this experiment containing iso-nitrogenous and iso-energy with different levels of zinc (0, 25, 50, 100, and 200 mg per kg). The initial means of body weight of body the fishes was 0.82 g per individual. The fishes were fed upon the diets at satiation, three times daily, for 12 weeks. The results indicated no zinc deficiency symptoms in this experiment. The daily growth rate, feed efficiency, survival rates and protein and lipid retention were not significantly different from all the diets. However, fish fed upon 25 mg Zn/kg diets produced the highest protein and total digestibility. The zinc contents of 25 mg Zn/kg diets gave the highest value in zinc accumulation in the bone, blood serum and eyes. The highest accumulation of the zinc in the liver occurred in 50 mg Zn/kg diets. There was increase in zinc content of the tissues after increasing levels of dietary zinc. Thus, Zn requirement for juvenile of giant gouramy was 25-50 mg/kg diets. Keywords: Osphronemus gouramy, Zinc, diet   ABSTRAK Penelitian ini dilakukan untuk mengetahui kebutuhan seng dalam pakan untuk benih ikan gurame.  Lima jenis pakan percobaan yang diuji mengandung iso-nitrogen dan iso-energi dengan kadar seng (Zn) yang berbeda (0, 25, 50, 100, and 200 mg per kg).  Bobot rata-rata benih yang digunakan adalah 0,82 g per ekor.  Ikan dipelihara selama 12 minggu dan diberi pakan 3 kali sehari.  Hasil penelitian menunjukkan bahwa tidak ada gejala defisiensi Zn.  Laju pertumbuhan harian, efisiensi pakan, kelangsungan hidup dan rentesi protein dan lemak tidak berbeda nyata untuk semua perlakuan.  Namun demikian, ikan yang diberi Zn sebanyak 25 mg/kg pakan menghasilkan kecernaan total dan protein tertinggi.  Perlakuan tersebut juga menghasilkan akumulasi Zn tertinggi dalam tulang, serum darah dan mata.  Akumulasi Zn tertinggi dalam hati diperoleh pada perlakuan 50 mg Zn/kg pakan.  Kandungan Zn dalam jaringan meningkat sejalan dengan meningkatnya kadar Zn dalam pakan. Dengan demikian, kebutuhan Zn bagi benih ikan gurame adalah sebanyak 25-50 mg /kg pakan. Kata kunci: Osphronemus gouramy, seng, pakan
GROWTH PERFORMANCE OF AFRICAN CATFISH (CLARIAS SP.) JUVENILE FED ON THE DIETS CONTAINING VARIOUS CHROMIUM CONTENT Aryansyah, H.; Mokoginta, I.; Jusadi, D.
Jurnal Akuakultur Indonesia Vol. 6 No. 2 (2007): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.584 KB) | DOI: 10.19027/jai.6.171-176

Abstract

This experiment was conducted to determine the effect of different dietary chromium level on the growth performance of catfish (Clarias sp.) juvenile. Five experimental diets contain different chromium level, namely diet A (as a control diet) 0.01; B 1.30; C 2.60; D 3.90 and E 5.20 mg/kg diet were used in this experiment. Fish with body weight of  5.57 ± 0.01 g/ind, fed on the experimental diet, twice a day, at satiation. Feeding trial was conducted for 60 days. Based on the evaluation of protein level of the whole body, protein and lipid retention, daily growth rate and feed efficiency, it was concluded that the optimum dietary chromium level for catfish  juvenile was 2.60 mg/kg diet. Keywords: chromium, growth, Clarias sp.   ABSTRAK Penelitian ini dilakukan untuk mengetahui pengaruh pemberian kromium dengan level berbeda terhadap pertumbuhan ikan lele (Clarias sp.) juvenil.  Lima jenis pakan yang mengandung kromium berbeda, yaitu pakan A  (kontrol) 0,01; B 1,30; C 2,60; D 3,90 dan pakan E 5,20 mg/kg digunakan dalam penelitian ini. Ikan dengan bobot 5,57 ± 0,01 g/ind., diberi pakan 2 kali sehari secara satiasi.  Pemeliharaan ikan dilakukan selama 60 hari.  Berdasarkan analisa retensi protein, kandungan protein dan retensi lemak dalam tubuh ikan lele, disimpulkan bahwa kadar kromium yang optimum untuk juvenil ikan lele adalah 2,60 mg/kg pakan. Kata kunci: kromium, pertumbuhan, Clarias sp.
EFFECT OF DIETARY PROTEIN LEVEL AND PROTEIN TO ENERGI RATIO ON THE GROWTH OF SWIMMING CRAB PORTUNUS PELAGICUS Serang, A. Malik; Suprayudi, M. Agus; Jusadi, D.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 6 No. 1 (2007): Jurnal Akuakultur Indonesia
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.619 KB) | DOI: 10.19027/jai.6.55-63

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The extending of protein and energy of the Swimming crab should be on certain limit that could be give maximum growth. The objective of this research are to determine of the protein level and optimum energy ratio of protein (C/P) at rajungan (Portunus pelagicus). The Crab-5 (C-5) stage used in the experiment, maintained in the topless (volume of 2 liters), with density of 1 crab/glass. There were seven of the experimental diets namely A (protein of 30%; C/P of 8 kkal), B (30%; 9.5), C (35%; 8), D (35%; 9.5), E (40%; 8), F (40%; 9.5) and G (45%; 8) with extending of food are 5 time of daily. Complete randomize design with 2 of factor and 3 of replication use in the experiment. The results of this experiment showed that interaction of the protein level and the energy ratio of protein is influence to the growth rate of daily and retention of lipid. The D is the best in give of the growth rate of daily, the consumption of food, the retention of protein, and the frequency of moulting (P
THE EFFECT OF DIFFERENT LEVELS OF VITAMIN E ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (DANIO RERIO) Utomo, N.B.P; Junior, M. Zairin; Yusuf, T.L.; Mokoginta, I.; Bintang, M.
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.367 KB) | DOI: 10.19027/jai.4.125-129

Abstract

This experiment was conducted to determine the dietary Vitamin E requirement for reproduction of broodstock zebrafish Danio rerio.  Four isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, C, and D with different levels of Vitamin E were fed to zebrafish broodstock.  The broodstock were cultivated in aquaria.  Diet A contained low dosage of Vitamin E (5 mg Vitamin E /kg diet), while diets B (62 mg Vitamin E /kg diet), C (125 mg Vitamin E /kg diet), and diet D (187 mg Vitamin E /kg diet), combined respectively with 1% n-3 fatty acids and 2% n-6 fatty acids.  Fish were fed ad satiation for 60 days using these diets.  During feeding period, gonad maturation stages were examined.  The dietary with different level of Vitamin E affected egg size, chemical content, total number of larvae, normal larvae, and survival rate of larvae produced, fecundity, fertilization rate, and hatching rate.  On the other hand, fish fed on experimental diets did not show any significance differences in the gonad somatic index, fecundity, fertilization rate, and hatching rate.  Fish fed on diet D produced the highest fecundity (633.65 ± 71.86 eggs/grams of fish), fertilization rate (92.3 ± 7.7%), and hatching rate (80.31 ± 9.8%).  The total lipid content of eggs were significant, ranging from D (39.7%), C (33.5%), B (32.1%), and A (29.5%).  At a dosage of 1% of dietary n-3 fatty acids and 2% n-6 fatty acids, zebrafish require 187 mg Vitamin E/kg feed in the diet for reproduction.  . Keywords:  vitamin E, reproductive performance, Danio rerio   ABSTRAK Penelitian ini ditujukan untuk mengetahui kebutuhan Vitamin E pada pakan induk ikan zebra (Danio rerio) untuk reproduksi.  Empat pakan perlakuan yang iso-protein (39%) dan iso-kalori (3.260 kcal digestible energy/kg pakan), dinamakan pakan A, B, C, dan pakan D dengan kandungan Vitamin E yang berbeda diberikan kepada induk ikan zebra.  Induk ikan dipelihara pada akuarium.  Pakan A mengandung Vitamin E terendah (5 mg Vitamin E/kg pakan), sedangkan pakan B mengandung Vitamin E 62 mg Vitamin E/kg pakan, pakan C asam mengandung Vitamin E 125 mg /kg pakan, dan pakan D mengandung Vitamin E 187 mg /kg pakan.  Semua pakan perlakuan mempunyai kombinasi asam lemak n-3 berbanding n-6 sebesar 1:2.  Ikan diberi pakan secara ad satiation selama 60 hari pemeliharaan.  Selama masa pemberian pakan, tingkat kematangan gonad diperiksa secara teratur.  Perbedaan kandungan vitamin E dalam pakan mempengaruhi fekunditas, derajat pembuahan telur, serta derajat penetasan telur.  Pakan perlakuan tidak mempengaruhi nilai gonad somatik indeks dan kelangsungan hidup larva umur 3 hari.  Pemberian pakan D menghasilkan fekunditas (633.65 ± 71.86  telur/gram induk), derajat pembuahan telur (92.3 ± 7.7%), serta derajat penetasan telur (80.31 ± 9,8%) terbaik.  Total kandungan lemak pada telur berbeda nyata, yaitu pada pakan D (39.7%), C(33.5%), B(32.1%), dan pakan A(29.5%).  Pada kandungan 1% asam lemak n-3 dan 2% asam lemak n-6, ikan zebra membutuhkan 187 mg Vitamin E/kg pakan untuk reproduksi yang normal.  Kata kunci:  Vitamin E, penampilan reproduksi, Danio rerio