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Biochemical Genetic Analysis Of Three Population Of Marble Grouper, Epinephelus polypekhadion Permana, Gusti Ngurah; Haryanti, Haryanti; Moria, Sari Budi; Giri, Nyoman Adiasmara; Sugama, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.32

Abstract

Genetic variations of marble groupers, Epinephelus polypekhadion were evaluated to know genetic performance of fish to support fish seed production. Fifty fish samples from three locations i.e. West Nusa Tenggara, South Sulawesi and East Java were collected for analysis. Genetic analysis has been examined through allozyme electrophoresis by using 11 enzymes (ADH, GPI, SDH, IDH, LDH, ME, PGM, 6PGD, MPI, SP, EST). The result showed that 16 loci were detected, and three of them were polymorphism enzymes namely Isocitric dehydrogenase (IDH*), Glucose Phosphate Isomerase (GPI-1*) and Esterase (EST-2*). One locus (EST-2) was polymorphic in the East Java and West Nusa Tenggara populations and three loci (EST-2, GPI-1 and IDH) were polymorphic in the South Sulawesi population. The heterozygosity ranged from 0.007 to 0.034. Rogers genetic distance between population pairs were ranged from 0.005 to 0.012 (average = 0.009). Differences between genetic populations were significant (P<0.05). East Java and West Nusa Tenggara populations have same gene pool.
Polimorfisme Enzim Glucose-6-Phosphate Isomerase pada Tiga Populasi Tuna Sirip Kuning (Thunnus albacares) Permana, Gusti Ngurah; Hutapea, Jhon H.; Moria, Sari Budi; Haryanti, Haryanti
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.163

Abstract

Samples of yellowfin tuna (Thunnus albacares) were taken from three locations Bali, North Sulawesi and North Maluku. The glucose-6-phosphate isomerase (GPI) was analyzed from liver using allozyme electrophoresis method. Polymorphism of GPI enzyme was observed and four alleles (A, B ,C, D) were found in Bali population, three alleles (A,B,C) were found in North Maluku and North Sulawesi populations. Heterozygosity values, from Bali, North Maluku and North Sulawesi were 0.419; 0.417; 0.143 respectively. Genetic distance between North Sulawesi and North Maluku were 0.029, and with Bali population was 0.353. These results indicated that North Maluku and North Sulawesi population were not separate by geographic barrier, therefore genetic distance of both populations was closed. However, those populations were genetically separated to the Bali population might be due to major hydrological barrier.
STUDY ON FRY PERFORMANCE OF BLACK TIGER SHRIMP Penaeus monodon WITH SPECIAL REFERENCE TO ITS MORPHOLOGY AND RNA/DNA RATIO ANALYSIS Haryanti, Haryanti; Mahardika, Ketut; Moria, Sari Budi; Permana, I Gusti Ngurah
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.262 KB) | DOI: 10.15578/iaj.1.2.2006.159-164

Abstract

Standard method to asses the performance of black tiger shrimp (Penaeus monodon) fry was needed for successful shrimp culture. The main purpose of this study was to determine standard method fry performance of P. monodon assesment based on its morphology and molecular RNA/DNA ratio analysis. Samples of P. monodon fry were collected from hatcheries in Bali, six hatcheries in East Java, three hatcheries in Central Java and six hatcheries in South Sulawesi. Each hatchery gave 25 appropriate sizes of fry samples taken from same tank culture. RNA/DNA ratio value was obtained from gene-quant measurement.  Result of this study shown that morphology performance of shrimp fry correlated with RNA/DNA ratio.  RNA/DNA ratio of shrimp fry from Bali hatcheries were obtained of 0.7121. Shrimp fry from hatcheries in East Java  showed  RNA/DNA value ranged between 0.2823-1.2132, while shrimp fry from hatcheries in Central Java and South Sulawesi  ranged between 1.1810-17478  and  0.1798-0.5116 respectively.
VARIASI MORFOMETRIK DAN ALLOZYME CALON INDUK RAJUNGAN, Portunus pelagicus DARI BEBERAPA PERAIRAN DI INDONESIA Permana, Gusti Ngurah; Moria, Sari Budi; Haryanti, Haryanti; Susanto, Bambang
Jurnal Riset Akuakultur Vol 1, No 2 (2006): (Agustus 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.771 KB) | DOI: 10.15578/jra.1.2.2006.235-244

Abstract

Sampel diambil dari empat populasi rajungan yang berbeda yaitu Sulawesi Selatan, Jawa Timur, Jawa Tengah, dan Bali. Penelitian ini dilakukan untuk mengetahui variasi morfometrik dan allozyme dari calon induk rajungan. Hasil yang diperoleh yaitu variasi genetik rata-rata keempat populasi sangat rendah (0,0025). Rajungan dari Jawa Tengah dan Bali mempunyai nilai heterosigositas tertinggi yaitu 0,004 sedangkan populasi Sulawesi Selatan dan Jawa Timur (0,001). Jarak genetik populasi Jawa Timur dan Bali (0,0013), kemudian Jawa Tengah (0,0016), dan Sulawesi Selatan (0,002). Uji analisis komponen utama (Principal component analysis, PCA), menunjukkan bahwa secara morfometrik rajungan jantan dan betina yang berasal dari populasi Cilacap-Jawa Tengah dan P. Saugi-Sulawesi Selatan dapat membentuk satu sub populasi yang sama, sebaliknya populasi asal Negara-Bali membentuk sub populasi tersendiri. Korelasi yang erat antara nisbah panjang dan lebar karapas terhadap bobot tubuh ditemukan pada populasi P. Saugi-Sulawesi Selatan dan Cilacap-Jawa Tengah sebaliknya pada populasi Negara-Bali mempunyai korelasi yang rendah.Samples were collected from South Sulawesi, Central Java, East Java, and Bali. Genetic variation from allozyme was consistently low in all populations (0.0025) This research aimed to know morphometric and allozyme variation of Swimming Blue Crab, Portunus pelagicus from Indonesian waters. Population from Central Java and Bali had the highest heterozigosity value (0.004) compare to those from South Sulawesi and East Java (0.001). Sample cluster according to the pair’s genetic distance showe that East Java and Bali population has the smallest value (0.0013). By contrast, the largest value was observed in Central Java (0.0016) and South Sulawesi population (0.002). Principal Component Analysis showed that morphometrically male and female swimming blue crabs from Saugi and Cilacap population can build one identical subpopulation On the other hand population originated from Negara made a separate subpopulation There high correlation between carapace length and width ratio on population of P. Saugi-South Sulawesi and Cilacap-Central Java, on the other hand, Negara-Bali population had a low correlation.
PROFIL PEMIJAHAN IKAN TUNA SIRIP KUNING, Thunnus albacares DALAM BAK TERKONTROL DENGAN ANALISIS MITOKONDRIA DNA (mt-DNA) Permana, Gusti Ngurah; Moria, Sari Budi; Hutapea, Jhon Harianto; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 4, No 2 (2009): (Agustus 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.495 KB) | DOI: 10.15578/jra.4.2.2009.157-167

Abstract

Variasi mitokondria DNA pada ikan tuna sirip kuning, Thunnus albacares menggunakan analisis RFLP (restriction fragment length polymorphism) dapat menyediakan data yang akurat dan memberikan bukti tentang profil pemijahan ikan tuna dalam bak terkontrol. Genotipe mt-DNA yang berasal dari induk dibandingkan dengan genotipe yang ada pada telur untuk memonitor dan mengetahui profil dari pemijahan ikan tuna dalam bak terkontrol. Telur dikumpulkan setiap pemijahan dari tahun 2004-2006. Profil pemijahan dari induk betina diamati dari jumlah genotipe yang ditemukan pada telur. Hasil dari penelitian ini adalah 49 induk yang dianalisis ditemukan 42 genotipe, 6 genotipe yang teramati ditemukan pada telur dan 4 diantaranya memiliki genotipe tunggal sedangkan satu genotipe (DABEA) dimiliki oleh dua induk. Prakiraan panjang cagak dan bobot induk pada saat memijah adalah 82,2-164 cm and 9,183-28,142 kg. Genotipe yang sama ditemukan hampir setiap hari pada saat sampling selama setahun. Hasil ini mengindikasikan bahwa ikan tuna sirip kuning dapat bertelur sepanjang tahun tergantung kepada suhu air dan kondisi pakan.Study of mitochondrial (mt-DNA) variations of yellowfin tuna, Thunnus albacares using (RFLP) restriction fragment length polymorphisms can provide evidence of spawning profile of the species in captivity. Mt-DNA genotypes of broodstock were compared with their eggs in order to monitor spawning profile. Spawned eggs were collected on every spawning from 2004 to 2006. The spawning profiles of these females were determined from the genotypes of the eggs. The result showed that from 49  broodstock individuals, 42 genotypes were observed, in which 6 genotypes were observed in their eggs and 4 of them established a single female’s identity and one type (DABEA) was shared by two females. Fork length and weight of broodstock female when spawning were ranging from 82.2–164 cm and 9.183-28.142 kg. The same genotypes were observed in almost every sampling session for one year period. The results indicate that the females are able to spawn at any time depending on optimum water temperature and food availability. 
PROFIL GENOTIP BENIH UDANG WINDU Penaeus monodon HASIL SELEKSI DENGAN KARAKTER TOLERAN TERHADAP INFEKSI WHITE SPOT SYNDROME VIRUS Haryanti, Haryanti; Fahrudin, Fahrudin; Wardana, Ida Komang; Moria, Sari Budi; Permana, Gusti Ngurah; Mahardika, Ketut
Jurnal Riset Akuakultur Vol 6, No 3 (2011): (Desember 2011)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.77 KB) | DOI: 10.15578/jra.6.3.2011.393-405

Abstract

Upaya seleksi pada benih udang windu dengan mengutamakan karakter toleran terhadap WSSV menjadi prioritas, agar diperoleh calon induk udang windu dengan karakter genotip yang lebih baik. Tujuan riset adalah mendapatkan profil genotip benih udang windu hasil seleksi dengan karakter toleran terhadap WSSV. Metode untuk mendapatkan benih udang terseleksi, diawali melalui pembenihan dengan menggunakan induk udang windu berasal dari alam (F-0), mengaplikasikan probiotik dalam pemeliharaan larva, biosecurity dan pemantauan infeksi virus. Uji tantang virus WSSV (LD-50) dilakukan dengan perendaman dan pemberian pakan (oral). Hasil yang diperoleh menunjukkan bahwa benih udang F-1 memberikan keragaan fenotip yang bervariasi (ukuran besar, sedang dan kecil). Benih udang F-1 berukuran besar, diperoleh 20,18%-35,04%, sedangkan ukuran sedang dan kecil masing-masing sebesar 43,28%0,49% dan 9,47%-21,66% dari populasi benih udang yang dihasilkan. Keragaan genotip benih F-1 menunjukkan keragaman genetik yang berbeda. Nilai heterozigositas pada benih F-1 pada udang dengan ukuran besar adalah 0,51, sedangkan ukuran sedang dan kecil masing-masing 0,45 dan 0,22. Hasil uji tantang dengan WSSV melalui perendaman maupun pemberian pakan (oral) terlihat ada perbedaan toleransi pada benih udang F-1. Dengan LD-50 menunjukkan bahwa pada udang ukuran kecil, mortalitas terjadi dalam waktu 48 jam (oral). Sementara, pada udang ukuran besar dan sedang, mengalami mortalitas dalam waktu 72 jam. Profil genotip benih udang yang toleran terhadap infeksi WSSV mengekspresikan pola gen yang lebih bervariasi
KARAKTER GENETIK DAN STRUKTUR POPULASI IKAN NAPOLEON, Ceilinus undulatus DI PERAIRAN INDONESIA Moria, Sari Budi; Haryanti, Haryanti; Permana, Gusti Ngurah; Slamet, Bejo
Jurnal Riset Akuakultur Vol 1, No 3 (2006): (Desember 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1109.534 KB) | DOI: 10.15578/jra.1.3.2006.315-323

Abstract

KARAKTER GENETIK DAN STRUKTUR POPULASI IKAN NAPOLEON, Ceilinus undulatus DI PERAIRAN INDONESIA
PENGGUNAAN BAKTERI PROBIOTIK Alteromonas sp. BY-g DALAM PEMELIHARAAN LARVA UDANG MELALUI PAKAN ALAMI DAN BUATAN Haryanti, Haryanti; Permana, Gusti Ngurah; Moria, Sari Budi; Giri, Nyoman Adiasmara; Sugama, Ketut
Jurnal Penelitian Perikanan Indonesia Vol 8, No 5 (2002): (Vol.8 No.5 2002)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9662.689 KB) | DOI: 10.15578/jppi.8.5.2002.55-66

Abstract

Upaya untuk menyederhanakan dan mengefisiensikan penggunaan bakteri slrain Alteromonas sp. BY-9 melalui pakan alami maupun pakan buatan telah dilakukan
MARKA GENETIK UNTUK VARIABILTTAS PERTUMBUHAN UDANG WINDU, Penaeus monodon DARI SUMBER INDUK BERBEDA MELALUI ANALISIS MT-DNA-RFLP Moria, Sari Budi; Haryanti, Haryanti; Ngurah Permana, l Gusti; Sugamal, Ketut
Jurnal Penelitian Perikanan Indonesia Vol 8, No 5 (2002): (Vol.8 No.5 2002)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6306.834 KB) | DOI: 10.15578/jppi.8.5.2002.1-9

Abstract

Dalam rangka perbaikan mutu genetik udang, diperlukan informasi marka genetik untuk mengetahui variabilitas pertumbuhan sebagai salah satu dasar seleksi individu. Tujian penelitian ini adalah untuk memperoleh marka genetir dari variabilitas pertumbuhan uoang windu yang nantinya dapat digunakan sebagai indikator dalam seleksi induk.
COLORATION CHARACTERISTIC AND POPULATION GENETIC ANALYSIS OF WILD-CAPTURED GIANT TIGER SHRIMP (PENAEUS MONODON) FROM ACEH TIMUR Indriatmoko, Indriatmoko; Hedianto, Dimas A.; Moria, Sari Budi; Tjahjo, Didik WH
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 3 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.485 KB) | DOI: 10.14710/ik.ijms.23.3.123-130

Abstract

Giant tiger shrimp (Penaeus monodon) has become a prime commodity in Indonesia which was produced by aquaculture and capture fisheries activities. Aceh Province, in this case mostly represented by Aceh Timur District, was well-known as the center of wild-captured-adult giant tiger shrimp. Several previous investigations had proved for its high-quality shrimp spawner in producing good eggs in quality and quantity under artificial spawning condition. Two main interesting points of wild giant tiger shrimp from Aceh Timur came from their coloration and population clusters. This report was aimed to provide that information pre-preliminary and highlighted quantitative information of coloration characteristic through RGB (Red Green Blue) and CIE Lab color space data analysis, as well as, 16S rDNA-PCR-RFLP genetic comparison among four population clusters in Aceh Timur Waters. The color analysis resulted in significant differences between wild-captured and pond-cultured giant tiger shrimp which produced R value 0.1524±0.0091 and 0.1268±0.0004, respectively. Total pixel analysis through L* a* b* color space has distinguished detailed differentiation between wild-captured and pond-cultured giant tiger shrimp acquired images. It is known that most of the wild-captured image pixels were concentrated in quadrant I (+a, +b) while pond-cultured in quadrant II (-a, +b) and III (-a, -b).Genotyping of represented samples from 4 population clusters, i.e. Aceh Tamiang, Langsa, Peudawa, and Julok produce 2 haplotype composite, AAA and AAB. Among 4 clusters, it was found that Julok has become the only cluster which has a different haplotype composite ratio (1:1) (D 0.0348, V 0,9501) from the others (4:1)(V 0.9504).