Articles

Percepatan Penyediaan Benih Sumber Kedelai Unggul Secara In Vitro Suminar, Erni; Sumadi, Sumadi; Mubarok, Syariful; Sunarto, Toto; Endah Rini, Nita Suswati
Agrikultura Vol 28, No 3 (2017): Desember, 2017
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.554 KB) | DOI: 10.24198/agrikultura.v28i3.15744

Abstract

ABSTRACTIn vitro method to fasten availability high quality soybean seedsSoybean is an important crop as a source of food and its demand has increased every year. Several new varieties of soybean have been generated, but the number is still limited. Furthermore, the infestation of pests and infection of diseases have also limited the new soybean varieties production as it increase the risk of growth failure. Therefore, methods to fasten the availability of high quality of soybean seeds need to be developed. One of which can be done through in vitro culture method. The objective of this study was to obtain the best type and the best concentration of cytokinin for the growth of soybean explants in vitro. The experiment was conducted in Seed Technology and Tissue Technology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. The experimental design used was Completely Randomized Design with 13 treatments and four replications. The basic media used were Murashige and Skoog (MS) + Vitamin B5 with addition of BAP (1.0 mg/l, 1.5 mg/l, and 2.0 mg/l), Kinetin (0.5 mg/l, 1.0 mg/l, and 1.5 mg/l), TDZ (0.01 mg/l, 0.1 mg/l, and 1.0 mg/l), and coconut water (10%, 15%, and 20%). The result showed that cytokinin types and concentrations gave different effect to soybean explant growth. The best treatment was demonstrated by BAP at the concentration of 1.5 mg/l as shown by the highest percentage of leaves and the number of shoots. However, it did not affect the percentage of buds produced by explant and the percentage of callus produced by explant.Keyword : BAP, Kinetin, TDZ, Coconut water, Soybean.AbstrakKedelai merupakan komoditas yang memegang peranan penting. Permintaan akan kedelai meningkat setiap tahunnya. Varietas kedelai baru yang bersifat unggul sudah banyak dihasilkan, namun jumlahnya masih terbatas. Tingginya serangan hama dan penyakit saat perbanyakkan benih di lapangan menyebabkan tingginya resiko kegagalan dalam pertumbuhan varietas baru tersebut. Sehingga perlu dilakukan percepatan penyediaan benih sumber varietas unggul di laboratorium secara in vitro. Tujuan penelitian ini adalah untuk mendapatkan jenis dan konsentrasi sitokinin yang terbaik untuk pertumbuhan eksplan kedelai in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan tiga belas perlakuan dan empat ulangan. Media dasar yang digunakan adalah Murashige dan Skoog (MS) + Vitamin B5 dengan penambahan BAP (1,0 mg/l; 1,5 mg/l; 2,0 mg/l), Kinetin (0,5 mg/l; 1,0 mg/l; 1,5 mg/l), TDZ (0,01 mg/l; 0,1 mg/l; 1,0 mg/l), dan air kelapa (10%; 15%; 20%). Hasil percobaan menunjukkan bahwa pemberian jenis dan konsentrasi sitokinin memberikan pengaruh yang berbeda terhadap pertumbuhan eksplan. Perlakuan terbaik diperoleh pada BAP dengankonsentrasi 1,5 mg/l yang ditunjukkan dengan tingginya persentase jumlah daun dan tunas yang terbentuk. Akan tetapi, penambahan sitokinin tersebut tidak memberikan pengaruh terhadap persentase eksplan dalam membentuk tunas dan persentase eksplan dalam membentuk kalus.Kata Kunci : BAP, Kinetin, TDZ, Air Kelapa, Kedelai
Regenerasi Berbagai Jenis Eksplan Nilam Klon Sidikalang dan Aplikasi Azotobacter pada Tahap Aklimatisasi Erni, Erni; Sobarna, Denny Sobardini; Nuraini, Anne; Mubarok, Syariful; Suryatmana, Pujawati; Sihombing, Yudhistari; Angel, Christine
Agrikultura Vol 27, No 2 (2016): Agustus, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.998 KB) | DOI: 10.24198/agrikultura.v27i2.9992

Abstract

AbstrakKondisi produktivitas nilam di Indonesia saat ini masih rendah, hal ini diduga disebabkan oleh rendahnya penggunaan benih unggul serta kendala ketersediaan benih yang tepat waktu, jumlah bibit yang seragam dan bebas penyakit, serta masih rendahnya mutu genetik tanaman. Sehingga perlu dilakukan upaya perbanyakan maupun peningkatan mutu genetik dengan aplikasi teknologiin vitro. Percobaan I: Pertumbuhan eksplan nilam pada komposisi sitokinin yang berbeda secara in Vitro (Rancangan Acak Lengkap pola faktorial), faktor pertama (jenis ekplan : mata tunas, pucuk, dan daun), sedangkan faktor kedua (0 mg/l sitokinin; 0,5 mg/l BAP; 1,0 mg/l BAP; 0,5 mg/l Zeatin; dan 1,0 mg/l Zeatin). Percobaan II: Multiplikasi stek mikro nilam secara in vitro (Rancangan Acak Lengkap). Percobaan III: Respon stekmikro terhadap inokulasi Azotobacter sp. pada tahap aklimatisasi (Rancangan Acak Kelompok) terdiri dari lima perlakuan (tanpa inokulasi, 1 ml, 2 ml dan 3 ml Azotobacter sp.). Hasil Percobaan I menunjukkan tidak terdapat interaksi antara jenis eksplan dengan jenis dan konsentrasi sitokinin. Penggunaan eksplan pucuk menghasilkan rata-rata jumlah tunas, pertambahan jumlah daun serta rata-rata bobot segar eksplan lebih baik serta penambahan 0,5 mg/l BAP pertambahan jumlah daun dan rata-rata bobot segar eksplan nilam lebih baik pada 8 MST. Percobaan II menunjukkan bahwa penggunaan 0,01 mg/l NAA dan 1 mg/l BAP merupakan perlakuan yang terbaik untuk multiplikasi tunas nilam pada peubah jumlah tunas dan jumlah daun. Percobaan III menunjukkan inokulasi Azotobacter sp. dengan dosis 3 ml memberikan rata-rata pertambahan jumlah daun, jumlah akar dan panjang tanaman relatif lebih baik.Kata kunci : Aklimatisasi, Azotobacter, Cytokinin, Eksplan, Nilam, Regenerasi
MULTIPLICATION EXAMINATION OF TURMERIC EXPLANTS USING AUXIN AND CYTOKININ TO MODIFIED MEDIA Azizah Romadhoni, Nur; Suminar, Erni; Nuraini, Anne; Mubarok, Syariful
Jurnal Penelitian Saintek Vol 24, No 1: April 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.683 KB) | DOI: 10.21831/jps.v24i1.19503

Abstract

This study was aimed at obtaining the type and concentration of cytokines as well as the optimal concentration of auxin for the multiplication of turmeric shoots in vitro. The trial was conducted in September 2017 until February 2018 at the Tissue Culture Seed Technology Laboratory of the Faculty of Agriculture, Padjadjaran University. The materials used in this study were Murashige and skoog modified multiplication medium, jelly, sterile distilled water, HgCl2, 70% alcohol, clorox, tween 80 and fungicide and bactericidal. Growth Regulating Substances (GRS) used are BAP, TDZ, Zeatin and NAA. The explant source used was derived from the shoot of turmeric clones from Bogor. The experimental design in this study was Completely Randomized Design (CRD) with seven treatments and four replications. The planting medium used was Murashige Skoog Modification (MS) with the addition of Benzyl Amino Purine (BAP) 9 mg L-1, Thidiazuron (TDZ) 1 mg L-1, Zeatin 0.1 mg L-1 and NAA (0.01 mg L-1, 1 mg L-1). The results show that the combination of BAP 9 mg L-1 and NAA 0.01 mg L-1 produced the highest shoot induction at 12 weeks after planting. Giving zeatin 0.1 mg L-1 and NAA 1 mg L-1 produces the highest shoot length at 12 weeks after planting.PENGUJIAN MULTIPLIKASI EKSPLAN KUNYIT DENGAN PENAMBAHAN AUKSIN DAN SITOKININ PADA MODIFIKASI MEDIAPenelitian ini bertujuan untuk mendapatkan jenis dan konsentrasi sitokinin serta konsentrasi auksin yang optimal untuk multiplikasi tunas kunyit secara in vitro. Percobaan dilaksanakan pada bulan September 2017 sampai Februari 2018 di laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Bahan yang digunakan dalam penelitian ini adalah murashige dan skoog modified multiplication medium, agar-agar, aquades steril, HgCl2, alkohol 70%, clorox, tween 80, fungisida, dan bakterisida. Zat Pengatur Tumbuh (ZPT) yang digunakan yaitu BAP, TDZ, Zeatin, dan NAA. Sumber eksplan yang digunakan yaitu berasal dari tunas kunyit klon asal Bogor. Rancangan percobaan pada penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan dan empat ulangan. Media tanam yang digunakan yaitu Murashige Skoog Modifikasi (MS) dengan penambahan Benzyl Amino Purine (BAP) 9 mg L-1, Thidiazuron (TDZ) 1 mg L-1, Zeatin 0,1 mg L-1, dan NAA (0,01 mg L-1, 1 mg L-1). Hasil percobaan menunjukkan bahwa kombinasi BAP 9 mg L-1 dan NAA 0,01 mg L-1 menghasilkan induksi tunas tertinggi pada 12 MST. Pemberian zeatin 0,1 mg L-1 dan NAA 1 mg L-1 menghasilkan panjang tunas tertinggi pada 12 MST.
Pengaruh Kombinasi Komposisi Media Tanam dan Konsentrasi Sitokinin terhadap Pertumbuhan Aglaonema Mubarok, Syariful; Salimah, A; Farida, Farida; Rochayat, Y; Setiati, Y
Jurnal Hortikultura Vol 22, No 3 (2012): September 2012
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK. Pertumbuhan dan kualitas Aglaonema dipengaruhi oleh beberapa faktor di antaranya media tanam dan aktivitas hormonal. Penggunaan media tanaman alternatif selain pakis dan hormon seperti sitokinin penting untuk diketahui. Tujuan penelitian ialah untuk mengetahui pengaruh kombinasi tiga komposisi media tumbuh sebagai media alternatif serta pemberian sitokinin terhadap pertumbuhan dan kualitas  Aglaonema Fit Langsit. Penelitian dilakukan di Rumah Kaca Fakultas Pertanian Universitas Padjadjaran mulai Bulan Mei sampai dengan Agustus 2008. Komposisi media tanam yang digunakan yaitu arang sekam, cocopeat, dan zeolit dengan perbandingan (2:2:1), (3:2:1), (4:2:1), dan sebagai kontrol digunakan pakis, humus, pasir malang, dan cocopeat (2:1:1:1) yang dikombinasikan dengan pemberian sitokinin dengan konsentrasi 0, 50, dan 100 µl/l yang disemprotkan pada daun setiap 2 minggu sekali. Hasil percobaan menunjukkan bahwa kombinasi arang sekam, cocopeat, dan zeolit (3:2:1) disertai sitokinin 50 µl/l memberikan pengaruh yang lebih baik dibandingkan perlakuan lainnya dalam memperpanjang dan memperlebar ukuran daun, sehingga dapat meningkatkan kualitas tanaman Aglaonema menjadi lebih rimbun dan kompak karena semakin meningkatnya ukuran daun.ABSTRACT. Mubarok, S, Salimah, A, Farida, Rochayat, Y,  and Setiati, Y 2012. The Effect of Growing Media Compositions and Cytokinin Concentrations on the Growth of Aglaonema. The growth and quality of Aglaonema is affected by several factors such as growing media and hormones. The using of alternative growing media and hormones such as cytokinin are urgently needed to be identified. The aim of experiment was to find out the effect of the combination of three growing media composition as an alternative growing medium with cytokinin on the growth and quality of Aglaonema Fit Langsit. This experiment was conducted in Greenhouse at the Faculty of Agriculture, Padjadjaran University from May to August 2008. The compositions of growing media consisted of the mix of carbonated rice hulls, cocopeat, and zeolite at three combinations (2:2:1, 3:2:1, and 4:2:1) and a control consisted of the mix of fern, humus, malang sands, and cocopeat (2:1:1:1). They were combined with cytokinins of 0, 50, and 100 µl/l. The results showed that the composition of carbonated rice hulls, cocopeat, and zeolite (3:2:1) combined with 50 µl/l cytokinin gave a better result than other treatments in increasing leaves length and leaves width, so it increases the quality of Aglaonema become more dense and compact due to by increasing the leaf size.
Multiplikasi Tunas Meriklon Kentang Pada Berbagai Jenis dan Konsentrasi Sitokinin Munggarani, Mia; Suminar, Erni; Nuraini, Anne; Mubarok, Syariful
Agrologia Vol 7, No 2 (2018)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v7i2.766

Abstract

Mericlone shoots are shoots of propagation using meristem explants which aim to produce seeds in a short time, large number, and disease-free seed. The aim of this experiment was to find out the best types and concentration of cytokinin to mericlone shoot multiplication of potato Jala Ipam variety. The experiment was carried out at Seed Technology Tissue Culture Laboratory of Agriculture, Padjadjaran University during November 2016 until February 2017. The experimental design was Completely Randomized Design with 13 treatments and 4 replications. Murashige and Skoog Medium with different concentration and types of cytokinine was used in this experiment. The result showed that the type and concentrations of cytokinin give the different effect on the mericlone shoot multiplication of potato Jala Ipam variety. MS medium with the additional of cytokinin 2 -iP 1.5 mg L-1 significantly increased the mericlone shoot multiplication of potato Jala Ipam variety at the variable number of leaves, number of nodes, and the number of branches.
Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi BAP dan NAA yang berbeda Lestari, Fitri Widya; Suminar, Erni; Mubarok, Syariful
Jurnal Agro Vol 5, No 1 (2018)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/1348

Abstract

Rendahnya produksi kentang di Indonesia disebabkan oleh ketersediaan benih kentang bermutu hasil dari metode konvensional yang kurang memadai. Metode kultur jaringan mampu menghasilkan bibit bermutu dan bebas virus, dalam jumlah banyak serta waktu yang singkat. Penelitian ini bertujuan untuk mendapatkan konsentrasi 6-benzylaminopurine (BAP) dan Naphthalene Acetic Acid (NAA) terbaik pada berbagai eksplan untuk pertumbuhan tunas meriklon kentang (Solanum tuberosum L.) Kultivar Atlantik secara in vitro. Percobaan dilaksanakan pada November 2016 sampai Februari 2017 di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan Acak Lengkap (RAL) digunakan sebagai rancangan percobaan yang terdiri dari dua unit percobaan terpisah yaitu unit eksplan meristem interkalar dan meristem apikal, masing-masing delapan perlakuan dan empat ulangan. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan penambahan BAP (0,0 mg L-1; 1 mg L-1; 1,5 mg L-1; 2 mg L-1) dan NAA (0,0 mg L-1; 0,5 mg L-1). Hasil menunjukkan konsentrasi BAP 1 mg L-1 merupakan perlakuan yang paling baik dalam menghasilkan jumlah tunas, cabang, daun dan buku pada eksplan meristem interkalar. Pada eksplan meristem apikal, penambahan BAP dan NAA belum mampu meningkatkan pertumbuhan eksplan. Meristem interkalar yang diberi auksin dan sitokinin berpotensi menjadi bahan alternatif eksplan meriklon kentang selain meristem apikal. The production of potato in Indonesia is low due to the limited number of high quality potato seeds produced from the conventional methods. The tissue culture methods can be used to produce high quality and virus-free seeds in more reliable number in a short time. Randomized Completely Design (RCD) was used as the experimental design consisted of two separate trial units namely intercalary meristem and apical meristem explants units with eight treatments and four replications of each. Murashige and Skoog (MS) was used as the base media added with BAP i.e. 0.0 mg L-1; 1 mg L-1; 1.5 mg L-1; 2 mg L-1 and NAA 0.0 mg L-1 and 0.5 mg L-1. Results showed that concentration of BAP 1 mg L-1 generated the best results on the number of shoots, branches, leaves and nodes on meristem intercalary explant. Meanwhile on apical meristem explant, the addition of BAP and NAA had not yet improved the explant growth. Intercalary meristem added with auxin and cytokinin is potential as alternative of mericlone explants material of potato besides the apical meristem.
Keberhasilan Persilangan Tomat Varietas Komersial (Lycopersicum esculentum L.) dengan Tomat Mutan Tahan Simpan Wiguna, Gungun; Rosalita, Elfira; Anas, Anas; Rostini, Neni; Mubarok, Syariful; Ezura, Hiroshi
Zuriat Vol 30, No 1 (2019): Latest Issue (April 2019)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.692 KB) | DOI: 10.24198/zuriat.v30i1.23205

Abstract

Post-harvest loss is an obstacle for tomato farmers that cause tomato farming being unprofitable. Utilization of mutant genes prolong tomato fruit shelf life through crossing is one of the best solutions to improve the fruit quality. The objective of this study was to determine the success of cross-pollination between commercial tomato varieties and shelf life of mutant tomatoes. Two mutant tomato lines Sletr1-1 and Sletr1-2, and one wild type strain were crossed with four commercial varieties (Intan, Mutiara, Ratna, and Mirah ). The results showed that the success rate of crossing ranged from 77.50% to 100%, with an average of 95.76%. The average maximum growth potential of seeds produced was 89.17% with the lowest value of 75% and the highest 98.5%. The most top success of crosses was built by a combination of crossing Ratna x Sletr1-2, and the combination of Mirah x Sletr1-1 showed the lowest percentage of the crossing. The Intan showed the highest success as female parent of crossing, while Mirah was the lowest production of the crossing. The plant height of all hybrid was smaller compared to the commercial varieties.
Pengaruh Kombinasi Komposisi Media Tanam dan Konsentrasi Sitokinin terhadap Pertumbuhan Aglaonema Mubarok, Syariful; Salimah, A; Farida, Farida; Rochayat, Y; Setiati, Y
Jurnal Hortikultura Vol 22, No 3 (2012): September 2012
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v22n3.2012.p251-257

Abstract

ABSTRAK. Pertumbuhan dan kualitas Aglaonema dipengaruhi oleh beberapa faktor di antaranya media tanam dan aktivitas hormonal. Penggunaan media tanaman alternatif selain pakis dan hormon seperti sitokinin penting untuk diketahui. Tujuan penelitian ialah untuk mengetahui pengaruh kombinasi tiga komposisi media tumbuh sebagai media alternatif serta pemberian sitokinin terhadap pertumbuhan dan kualitas  Aglaonema Fit Langsit. Penelitian dilakukan di Rumah Kaca Fakultas Pertanian Universitas Padjadjaran mulai Bulan Mei sampai dengan Agustus 2008. Komposisi media tanam yang digunakan yaitu arang sekam, cocopeat, dan zeolit dengan perbandingan (2:2:1), (3:2:1), (4:2:1), dan sebagai kontrol digunakan pakis, humus, pasir malang, dan cocopeat (2:1:1:1) yang dikombinasikan dengan pemberian sitokinin dengan konsentrasi 0, 50, dan 100 µl/l yang disemprotkan pada daun setiap 2 minggu sekali. Hasil percobaan menunjukkan bahwa kombinasi arang sekam, cocopeat, dan zeolit (3:2:1) disertai sitokinin 50 µl/l memberikan pengaruh yang lebih baik dibandingkan perlakuan lainnya dalam memperpanjang dan memperlebar ukuran daun, sehingga dapat meningkatkan kualitas tanaman Aglaonema menjadi lebih rimbun dan kompak karena semakin meningkatnya ukuran daun.ABSTRACT. Mubarok, S, Salimah, A, Farida, Rochayat, Y,  and Setiati, Y 2012. The Effect of Growing Media Compositions and Cytokinin Concentrations on the Growth of Aglaonema. The growth and quality of Aglaonema is affected by several factors such as growing media and hormones. The using of alternative growing media and hormones such as cytokinin are urgently needed to be identified. The aim of experiment was to find out the effect of the combination of three growing media composition as an alternative growing medium with cytokinin on the growth and quality of Aglaonema Fit Langsit. This experiment was conducted in Greenhouse at the Faculty of Agriculture, Padjadjaran University from May to August 2008. The compositions of growing media consisted of the mix of carbonated rice hulls, cocopeat, and zeolite at three combinations (2:2:1, 3:2:1, and 4:2:1) and a control consisted of the mix of fern, humus, malang sands, and cocopeat (2:1:1:1). They were combined with cytokinins of 0, 50, and 100 µl/l. The results showed that the composition of carbonated rice hulls, cocopeat, and zeolite (3:2:1) combined with 50 µl/l cytokinin gave a better result than other treatments in increasing leaves length and leaves width, so it increases the quality of Aglaonema become more dense and compact due to by increasing the leaf size.
Penghambatan Respons Etilen pada Mawar Potong Melalui Modifikasi Larutan Perendam, 1-MCP, dan Sitokinin Mubarok, Syariful; Nursuhud, .; Suminar, Erni; Viola, Venny Revia
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 1 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.816 KB) | DOI: 10.18343/jipi.23.1.60

Abstract

Roses (Rosa hybrida Hort.) are the popular flowering plants in Indonesia, however, some problems on postharvest cut roses are faced, such as short of flower longevities. The freshness or longevity of cut flowers can be maintained for a longer time by using vase solution, inhibition of ethylene responses ie with the use of 1-Methylcyclopropene (1-MCP) and cytokinin. The objective of this study is to determine the effect of 1-MCP and cytokinin combined with in a vase solution on the flower longevity dan quality of cut roses. The experiment was conducted using a Completely Randomized Design consisting of nine treatments, cinsisting of: control; combination of sucrose + NaOCl + cytokines (1% + 0.01% + 20 ppm; 2% + 0.01% + 20 ppm; 1% + 0.05% + 20 ppm; and 2% + 0.05% + 20 ppm) and combination of sucrose + NaOCl + cytokinin (1% + 0.01% + 1 ppm; 2% + 0.01% + 1 ppm; 1% + 0.05% + 1 ppm; and 2% + 0.05% + 1 ppm). The results showed that the composition of 1% sucrose + 0.05% NaCl + cytokinin solution 20 ppm gave the best results in maintaining the angle of petals, reducing the increase of flower diameters, retaining flower petal colors, and making flower longevities up to 11.56 days.
RESPONSE OF TURMERIC EXPLANT ON CYTOKININ AND AUXIN IN MURASHIGE AND SKOOG Indriani, Mita; Suminar, Erni; Wicaksana, Noladhi; Sobardini, Denny; Sulistyaningsih, Sulistyaningsih; Nuraini, Anne; Nursuhud, Nursuhud; Mubarok, Syariful
Jurnal Penelitian Saintek Vol 24, No 1: April 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.683 KB) | DOI: 10.21831/jps.v24i1.19784

Abstract

This study was aimed at determining the concentration of several types of cytokinins and auxin for the induction of turmeric shoots in vitro. The research was conducted at the Tissue Culture Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University, Jatinangor. The study was conducted from October 2017 to February 2018. The source of planting material is in the form of shoots from the turmeric rhizome. The source of explants or planting material came from the field collected at the Tissue Culture Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University. Explants were taken from rhizome buds with a size of 0.6-2.0 cm. The experiment used a Completely Randomized Design which was analyzed using the Student’s T-test method. The number of experimental and control groups in this study were seven groups. Variation in treatment with different BAP, thidiazuron, zeatin, and NAA concentrations in each group. The results show that Thidiazuron 1 mgL-1 + NAA 1 mgL-1 gives better results on the percentage of live explants and number of shoots on turmeric plants (Curcuma domestica Val.) Clones 41 at the age of 14 weeks after planting.RESPONS EKSPLAN KUNYIT PADA SITOKININ DAN AUKSIN DALAM MEDIA MURASHIGE DAN SKOOGPenelitian ini bertujuan untuk mendapatkan salah satu konsentrasi dari beberapa jenis sitokinin dan auksin untuk induksi tunas kunyit secara in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor. Waktu pelaksanaan penelitian ini dimulai pada awal bulan Oktober 2017 sampai bulan Februari 2018. Sumber bahan tanam berupa tunas dari rimpang tanaman kunyit. Sumber eksplan atau bahan tanam berasal dari lapangan yang dikoleksi di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Eksplan diambil dari mata tunas rimpang dengan ukuran 0,6-2,0 cm. Percobaan menggunakan Rancangan Acak Lengkap yang dianalisis menggunakan metode Student’s T-test. Jumlah kelompok eksperimen dan kontrol dalam penelitian ini adalah tujuh kelompok. Variasi perlakuan dengan penambahan konsentrasi BAP, thidiazuron, zeatin, dan NAA yang berbeda pada setiap kelompok. Hasil penelitian ini menunjukkan bahwa Thidiazuron 1 mgL-1 + NAA 1 mgL-1 memberikan hasil yang lebih baik pada persentase eksplan hidup dan jumlah tunas pada tanaman kunyit (Curcuma domestica Val.) klon 41 pada umur 14 MST (Minggu Setelah Tanam).
Co-Authors -, Kusumiyati A Salimah Agung Rahmadi Akbar, Rachmad Amalia, Inneke Amalia, Inneke Anas Anas, Anas ANNE NURAINI Anni Yuniarti, Anni Ariyanto, Nur Budi Ariyanto, Nur Budi Arsri, Mustika Azizah Romadhoni, Nur Bambang Nurhadi Christine Angel, Christine Deninta, Nedya Denny Sobardini Sobarna, Denny Sobardini Denny Sobardini, Denny Dinnur Afiifah, Dinnur Elma, Tiara Elma, Tiara Endah Rini, Nita Suswati Endah Yulia Erni Erni ERNI SUMINAR Ezura, Hiroshi Falah, Risa Nurul Farida Farida Fauzi, Ardika Albi Fauzi, Ardika Albi Fauzi, Ardika Albi Fitrianti Widya Lestari, Fitrianti Widya Gungun Wiguna Hadiwijaya, Yuda Ine Elisa Putri, Ine Elisa Jajang Sauman Hamdani Kusumiyati Kusumiyati, Kusumiyati Lestari, Fitri Widya Mira Ariyanti Mita Indriani Muhardiono, Iman Munggarani, Mia Neni Rostini Ningrum, Ayu Ratna Noladhi Wicaksana Nursuhud, . Nursuhud, Nursuhud Nursuhud, Nursuhud Oktavia, Ade Risti Pakki, Siti Rakhmah Tenrisui Pujawati Suryatmana Purba, Lamro Qanit, Muhamad Abdilah Hasan Qanit, Muhammad Abdilah Hasan Qonit, Muhammad Abdilah Hasan Qonit, Muhammad Abdillah Hasan Rani Andriani Budi Kusumo Rezaldi, Firman Rija Sudirja Rizky, Wieny Rosalita, Elfira Rosniawati, Santi Rufaidah, Fathi Rufaidah, Fathi Santi Rosniawaty Saragih, Erika Wahyuni Sitepu, Rika Bhernike Sudarjat Sudarjat Sulistyaningsih Sulistyaningsih SUMADI SUMADI Toto Sunarto Vijaya Isnaniawardhani Viola, Venny Revia Wawan Sutari, Wawan Wieny Marma Jaya, Wieny Marma Y Rochayat Y Setiati Yudhistari Sihombing, Yudhistari