Articles

Incidence of HPV Infection in Oral Squamous Cell Carcinoma and Its Association with the Presence of p53 & c-myc Mutation : A Case Control Study in Muwardi Hospital Surakarta Prayitno, Adi; Rukmo, Mandojo; Mudigdo, Ambar; Dalono, Dalono; Asnar, Elyana; Rahayu, Retno Puji; Purwanto, Joko Agus; Puspaningsih, Ni Nyoman Tri; Kuntoro, Kuntoro; Harjanto, Harjanto; Putra, Suhartono Taat
Journal of Dentistry Indonesia Vol 17, No 2 (2010): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.302 KB) | DOI: 10.14693/jdi.v17i2.44

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Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. To amplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91% OSCC patient with HPV infection without mutation in c-myc gene. Chi  square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC.DOI: 10.14693/jdi.v17i2.44
The Role of Heat Shock Proteins in Pathogenesis of Oral Squamous Cell Carcinoma Prayitno, Adi; Rukmo, Mandojo; Mudigdo, Ambar; Dalono, J. B.; Asnar, Elyana; Rahayu, Retno Puji; Purwanto, Joko Agus; Puspaningsih, Ni Nyoman Tri; Kuntoro, Kuntoro; Harjanto, Harjanto J. M.; Putra, Suhartono Taat
Journal of Dentistry Indonesia Vol 16, No 2 (2009): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2754.761 KB) | DOI: 10.14693/jdi.v16i2.91

Abstract

Cell in the distress situation, denaturation of proteins may occur, and may also respond by expressing stress proteins. However, such homeostasis effort does not always succeed and even may lead to disease, including cancer. In distress situation also ensue much protein misfolding. Objective: This research were to explain the role of heat shock protein 40 (Hsp40) and Hsp70 in pathogenesis of occurred oral squamous cell carcinoma (OSCC) patient which realized human papilloma virus (HPV) infection. Material and Method: Tissue biopsy frozen section were taken from BOSC and OSCC patients was cut into three part. Parrafin blocks were made from cutting I, which was subsequently stains with HE to ascertain the type of neoplasm. Cutting II was subjected to DNA isolation. The DNA isolation results were subjected to PCR to amplify L1-HPV gene for fixed the HPV stressoor. Protein isolation was treated from Cutting III, folloewd with Blottdot test by using antibody monoclonal anti Hsp40 and Hsp70 and continued with measurement using densitometer to find the concentration of Hsp40 and Hsp70. The collected data were analyzed with F Test (Manova) and discriminant analysis. Result: This experiment showed the differences in concentration of Hsp40 (p<=0,070) and Hsp70 (p<=0,006) between beningn oral squamous cell (BOSC) and OSCC patients which realized HPV infection. Conclusion: This experiment proved that OSCC patients which realized HPV infection indicated an up regulated of Hsp70 concentration, so that there was occurs misfolding of the proteins cell. The misfolding was ensue obstacle of apoptosis and to raise cell proliferation which to storm carcinogenesis. An up regulated of Hsp40 was role as co-chaperone.DOI: 10.14693/jdi.v16i2.91 
Histopathology of Helicobacter pylori in Chronic Dyspepsia Patients Nur, Wildan; Kusnanto, Paulus; Pramana, Triyanta Yuli; Harnomo, Michael Tantoro; Oyong, Oyong; Mudigdo, Ambar
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 3, Desember 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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ABSTRACTBackground: Helicobacter pylori (H. pylori) are the most common infection found in dyspepsia cases. This infection is almost always found in digestive tract inflammation and commonly develops into chronic gastritis. Meanwhile, chronic gastritis is a condition assumed as the early event in pathological abnormalities of the stomach which finally may develop into carcinoma of the gaster. In Indonesia, data describing the incidence of H. pylori infection based on the histopathological appearance, location of specimen collection, inflammatory degree, and age of chronic dyspepsia patients is not yet available. The aim of this study is to determine the incidence of H. pylori based on histopathology appearance in chronic dyspepsia patients in Moewardi Hospital Surakarta.Method: This study is a cross sectional descriptive study by performing endoscopy-biopsy and histopathology examination to chronic dyspepsia patients who came to Gastroenterohepatology Clinic, Department of Internal Medicine, Moewardi Hospital, Surakarta on 1 January 2009 - 31 December 2010.Results: More than 90% subjects were > 40 year old with the distribution of majority patients were 46-55 year old (32.43%). The most commonly found endoscopic appearance in subjects with positive H. pylori was superficial chronic gastritis (81.08%) with mild inflammatory degree (64.86%) and majority located in the antrum 97.3%.Conclusion: The proporsion of H. pylori infection in male and female was almost equal and was mostly found in the age group of 46-55 year old. This infection frequently happens in chronic dyspepsia who has histopathologic appearance of superficial chronic gastritis with mild inflammatory degree in the antrum area. Keywords: Helicobacter pylori, superficial chronic gastritis, age
Health Belief Model on the Determinants of Human Papilloma Virus Vaccination in Women of Reproductive Age in Surakarta, Central Java Fitriani, Yulia; Mudigdo, Ambar; Andriani, Rita Benya
Journal of Health Promotion and Behavior Vol 3, No 1 (2018)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Cervical cancer is a type of cancer that develops in a woman's cervix. Cervical cancer is caused by a human papillomavirus (HPV). Cervical cancer ranked fourth against most women's cancer. The incidence of cervical cancer was estimated at 528,000 (7.9%) cases worldwide causing 266,000 deaths in 2012. The HPV vaccine can protect women from HPV infection, thus lowering the risk of cervical cancer. This study aimed to analyze the determinants of HPV vaccination in women of reproductive age in Permata Harapan Oncology Clinic, Surakarta, Central Java, using Health Belief Model.Subjects and Method: This was an analytic observational study with a case-control design. This study was conducted at Permata Harapan Oncology Clinic, Surakarta, from January to February 2018. A total study of 200 study subjects was selected by fixed disease sampling. The dependent variable was HPV vaccination. The independent variables were knowledge, perceived seriousness, perceived susceptibility, perceived barrier, perceived benefit, family income, and family support. The data were collected by questionnaire and analyzed by a multiple logistic regression.Results: HPV vaccination increased with better knowledge (OR=7.97; 95% CI= 1.50 to 42.38; p= 0.015), perceived seriousness (OR=22.81; 95% CI= 6.06 to 85.86; p<0.001), perceived susceptibility (OR=4.03; 95% CI= 1.25 to 13.09; p=0.020), , perceived benefit (OR­=6.57; 95% CI= 1.88 to 22.98; p=0.003), family income (OR=5.32; 95% CI= 1.57 to 18.07; p=0.007), and family support (OR=6.86; 95% CI= 1.55 to 30.36; p= 0.011). HPV vaccination decreased with perceived barrier (OR=0.14; 95% CI= 0.04 to 0.51; p=0.003).Conclusion: HPV vaccination increases with better knowledge perceived seriousness, perceived susceptibility, perceived benefit, family income, and family support, but decreases with perceived barrier.Keywords: HPV vaccination, knowledge, perceived seriousness, perceived susceptibility, perceived barrier, perceived benefit, family income, family supportCorrespondence: Yulia Fitriani. Masters Program in Public Health, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java. Email: yuliafitriani38@gmail.com. Mobile: 085655708946.Journal of Health Promotion and Behavior (2018), 3(1): 16-26https://doi.org/10.26911/thejhpb.2018.03.01.02 
Hubungan Konsumsi Fast Food dan Soft Drink dengan Kejadian Obesitas pada Remaja Usia 15-17 Tahun Barokah, Falah Indriawati; Mudigdo, Ambar; Prayitno, Adi
Jurnal Gizi dan Kesehatan Vol 1, No 1 (2014)
Publisher : Program Studi Ilmu Gizi Pascasarjana Universitas Sebelas Maret

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Abstract

Latar Belakang : Fast food dan soft drink keduanya banyak mengandung gula, terutama gula buatan. Gula buatan terbukti tidak baik untuk kesehatan karena dapat menyebabkan obesitas jika dikonsumsi terus menerus. Sehingga penulis tertarik untuk mengetahui hubungan konsumsi fast food dan soft drink dengan kejadian obesitas pada remaja umur 15-17 tahun.Metode : Penelitian ini menggunakan rancangan observasional analitik dengan pendekatan cross sectional. Populasi pada penelitian ini adalah seluruh siswa kelas X dan XI SMA Muhammadiyah 3 Jakarta dengan jumlah sampel 105 siswa. Teknik pengumpulan data dengan food frequency questionnaire dan formulir food recall. Data yang terkumpul dianalisis dengan Regresi Linear Berganda.Hasil : Terdapat hubungan yang signifikan antara konsumsi fast food dengan kejadian obesitas remaja (β = 0,111) dan (p = &lt;0,001) dengan persamaan (Y = 0,1X). Terdapat hubungan yang signifikan antara selera konsumsi soft drink dengan kejadian obesitas remaja (β = 0,05) dan (p = 0,018) dengan persamaan (Y = 0,05X). Semakin banyak remaja mengkonsumsi soft drink, dan fast food maka semakin tinggi risiko kejadian obesitas pada remaja umur 15-17 tahun. Kadar kortisol rata-rata pada siswa obesitas (A) dan non obesitas (B) berbeda tidak signifikan (A:B = 9,47:9,76).Kesimpulan : Sehingga kesimpulan yang didapat pada penelitian ini adalah bahwa fast food dan soft drink keduanya banyak mengandung karbohidrat. Selanjutnya kelebihan karbohidrat akan diubah menjadi asam lemak oleh hormon kortisol yang ditandai dengan terjadinya obesitas. Keywords : Fast Food, Soft Drink, Obesitas
Effect of Breast Care and Oxytocin Massage on Breast Milk Production: A study in Sukoharjo Provincial Hospital Rahayuningsih, Tutik; Mudigdo, Ambar; Murti, Bhisma
Journal of Maternal and Child Health Vol 1, No 2 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: The prevalence of exclusive breast feeding was 39.05% in Sukoharjo in 2015, which is far bellow the national target of 80%. A study has shown that massage oxytocin increases oxytocin (OT) hormone release, and eventually decreases adrenocorticotropin hormone (ACTH), nitric oxide (NO), and beta-endorphin (BE). This OT hormone release will increase milk ejection, which facilitate milk production. This study aimed to investigate the effect of breast care and oxytocin massages on breast milk production in post–partum mothers.Subjects and method: This was a Randomized Controlled Trial (RCT), conducted at Sukoharjo Hospital, Central Java 19 October to November 18, 2016. A total of 90 post – partum mothers were selected at random and then allocated into breast care group and oxytocin massage group. The dependent variable was breast milk production. The independent variable was breast care and oxytocin massage. Changes in breast milk production before and after intervention between the two groups were tested by Mann-Whitney test.Results: The increase in breast milk production in breast care and oxytocin massage group (mean = 17.37, SD = 9.70) was larger than that of the control group (mean = 1.58, SD = 1.69), and it was statistically significant (p <0.001).Conclusion: Breast care and oxytocin massage can significantly increase breast milk production. Post-partum mothers are recommended to practice breast care and oxytocin massage, in order to increase breast milk production.Keywords: breast care, oxytocin massage, breast milk production.Correspondende: Tutik Rahayuningsih. School of Health Polytechnics, Poltekkes Bhakti Mulia, Surakarta. Email: tutikrahayu_abm@yahoo.co.idJournal of Maternal and Child Health (2016), 1(2): 101-109https://doi.org/10.26911/thejmch.2016.01.02.05
Effect of Hormonal Contraceptive on Sexual Life, Body Mass Index, Skin Health, and Uterine Bleeding, in Women of Reproduction Age in Jombang, East Java Yosin, Esti Pratiwi; Mudigdo, Ambar; Budhiastuti, Uki Retno
Journal of Maternal and Child Health Vol 1, No 3 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Injection hormonal contraceptive can be distinguished into DMPA (depo medroxyprogesterone acetate) and combination. DMPA injection is administered in single dose of 150 mg/mL intramuscular every 12 week. It was hypothesized that DMPA injection resulted inadvertent effect such as lowered sexual life quality, increased body mass index, lowered quality of skin health, and abnormal uterine bleeding. This study aimed to examine the effects of DMPA injection on sexual life, body mass index, skin health, and abnormal uterine bleeding.Subjects and Method: This was analytical observational study with retrospective cohort design. This study was conducted in Jombang, East Java. A total sample of 149 women of reproductive age consisting of 99 DMPA injection contraceptive users and 50 non hormonal contraceptive users was selected for this study, by stratified random sampling. The dependent variables were sexual life quality, body mass index, quality of skin health, and uterine bleeding. The independent variable was DMPA injection contraceptive use and age. The data were collected by a set of questionnaire. Sexual life quality was measured by female sexual function index (FSFI). The data were analyzed by multiple logistic regression.Results: Use of hormonal contraceptive (OR= 20.17; 95% CI = 6.62 to 61.42; p<0.001) and age 30-35 years old (OR=17.51; 95% CI = 5.74 to 53.38; p<0.001) increased the risk of low quality of sexual life. Age 30-35 years old lowered (OR=0.16; 95% CI =0.07 to 0.36; p<0.001) and hormonal contraceptive (OR= 4.25; 95% CI = 1.95 to 9.30; p<0.001) increased the risk of low quality of skin health. Hormonal contraceptive (b=2.93; SE=0.23; p<0.001) and age 30-35 years old (b=0.70; SE=0.24; p<0.001) increased abnormal uterine bleeding. Hormonal contraceptive (b=5.75; SE=0.54; p<0.001) and age 30-35 years old (b=5.05; SE= 0.55; p<0.001) increased body mass index among women of reproductive age.Conclusion: Use of injection hormonal contraceptive lowers the quality of social life, lowers the quality of skin health, increases body mass index, and increases abnormal uterine bleeding.Keywords: hormonal contraceptive injection, sexual life, skin health, body mass index, uterine bleedingCorrespondence: Esti Pratiwi Yosin. School of Health Sciences Insan Cendekia Medika, Jombang, East Java. Email:estipratiwi77@gmail.com.Journal of Maternal and Child Health 2016, 1(3): 146-160https://doi.org/10.26911/thejmch.2016.01.03.02
Age, Parity, Physical Activity, Birth Weight, and the Risk of Perineum Rupture at PKU Hospital in Delanggu, Klaten, Central Java Hastuti, Tri Ari; Mudigdo, Ambar; Budihastuti, Uki Retno
Journal of Maternal and Child Health Vol 1, No 2 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Physiologic vaginal delivery of offspring may cause spontaneous unintended perineum tear (rupture) with varying degrees. Perineum rupture is the second leading cause of post-partum bleeding after uterine atony. The purpose of this study is to analyze the relationship between age, parity, physical activity, birth weight, and the risk of perineum rupture.Subject and Methods: This was an analytic observational study with cross sectional design. This study was carried out at PKU Hospital, Delanggu, Klaten, Central Java. A total of 78 mothers giving birth at the maternity ward were selected for this study stratified random sampling. The independent variables were age, parity, physical activity, and birth weight. The dependent variable was perineum rupture. The data were collected by interview and observation, and then analyzed using path analysis model.Results: Maternal age ≥ 35 years old (b = 3.36; 95%CI = 0.91 to 5.80; p = 0.007) increased the risk of perineum rupture, and it was statistically significant. Sufficient physical activity (b =-3.16; 95% CI =-5.05-1.27 ; p = 0.001) and multiparity (b =-4.05; 95% CI =-6.62 to -1.50; p = 0.002) decreased the risk of perineum rupture, and it was statistically significant. Birth weight did not show significant effect on the risk of perineum rupture (b = 1.13; 95%CI = 0.97 to 3.24; p = 0.291). Maternal employment status did not show its effect on physical activity level.Conclusion: Maternal age ≥ 35 years old increased the risk of perineum rupture. Sufficient physical activity and multiparity decreased the risk of perineum rupture. Special care should be taken on these two risk factors when assisting birth delivery in order to prevent perineum rupture.Key words: age, parity, physical activity, birth weight, perineum rupture.Correspondence: Tri Ari Hastuti. Masters Program in Public Health, Sebelas Maret University, Surakarta. Email: Triari1987@gmail.comJournal of Maternal and Child Health (2016), 1(2): 93-100https://doi.org/10.26911/thejmch.2016.01.02.04                          
Effect of Menopause Duration and Biopsychosocial Factors on Quality of life of Women in Kediri District, East Java Sari, Nunik Ike Yunia; Adriani, Rita Benya; Mudigdo, Ambar
Journal of Maternal and Child Health Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Menopause is defined as the point in time when menstrual cycles permanently cease due to the natural depletion of ovarian oocytes from aging. Studies have shown,  menopause causes decrease in quality of life and a positive correlation between menopausal symptoms and  quality of live. This study aimed to determine the effect of menopause duration and biopsychosocial factors on quality of life of  women.Subjects and Method: This study was an analytic study using cross-sectional design. The study was conducted in Bendo Community Health Center, Kediri District, East Java, from 8–31 March 2017. A sample was of 105 menopausal women was selected for this study by proportionate random sampling. The dependent variable was quality of life. Quality  of  life  encompassed several constructs including physical, functional, emotional, social, and cognitive variables. The independent variables were duration of menopause, Body Mass Index (BMI), education, family support, and family income. The data were collected by pre-tested questionnaire, and analyzed by path analysis.Results: Quality of life was affected by menopause duration (b=2.19; SE=0.38; p<0.001), education (b=6.72; SE=1.72; p<0.001), family support (b=0.42; SE=0.17; p=0.011), BMI (b=0.71; SE=0.27; p=0.010), and family income (b= 0.13; SE= 1.60; p= 0.936). BMI was affected by education (b= 1.87; SE= 0.50; p<0.001).Conclusion: Quality of life is directly affected by menopause duration, education, family support, BMI, and family income. Quality of life is indirectly affected by education.Keywords: menopause duration, biopsychosocial factors, quality of life, path analysisCorrespondence: Nunik Ike Yunia Sari. Masters Program in Public Health, Sebelas Maret University, Surakarta. Email: nunikike@yahoo.com. Mobile: +6282257969278.Journal of Maternal and Child Health (2017), 2(2): 125-136https://doi.org/10.26911/thejmch.2017.02.02.04
Analysis of Maternal Mortality Determinants in Bondowoso District, East Java Fransiska, Ratna Diana; Respati, Supriyadi Hari; Mudigdo, Ambar
Journal of Maternal and Child Health Vol 2, No 1 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Data from Population Inter-Census Survey (Survei Penduduk Antar Sensus,  SUPAS) 2015 showed that maternal mortality ratio was 305 per 100,000 live-births in Indonesia. Sustainable Development Goals (SDGs) set 70 per 100,000 live-births as the target for maternal mortality ratio to be achived by 2030. This study aimed to analyze the determinants of maternal mortality in Bondowoso district, East Java.Subjects and Method:This was an analytic observational study with cross-sectional design. This study was carried out at 17 Community Health Centers, in Bondowoso, East Java from February to March 2017. A sample of 117 study subjects, consisting of 39 cases of maternal death and  78 control, was selected for this study by fixed disease sampling. The dependent variable was incidence of maternal death. The independent variables were maternal education, maternal employment status, antenatal care  visit, complication, late model, and other pregnancy risk factors. The data were collected from the obstetric and medical record, as well as a set of questionnaire. The data were analyzed by path analysisResults: Determinants of maternal death included late decision making (b=2.37; 95% CI=0.81 to3.93; p=0.003), late transfer to the hospital (b=2.35; 95%CI =-0.21to4.91; to p=0.072), late handling at the hospital (b=2.36; 95%CI=-0.19to 4.91; p=0.069), and  complication (b=2.5; 95%CI=1.41to3.62; p<0.001). Complication was determined by completeness of antenatal visits (b=-1.01; 95%CI=-1.94to-0.09; p=0.032), and existence of pregnancy risk factor (b=1.90; 95%CI=1.01to 2.78; p=<0.001). Pregnancy risk factors was determined by completeness of antenatal visit (b=-1.09; 95%CI =-1.99to-0.19; p=0.018), maternal education (b=-0.47; 95%CI=-0.85to-0.07; p=0.020), and maternal employment status (b=0.14; 95%CI=-0.17to0.45; p=0.369). Antenatal visit was determined by maternal education (b=0.54; 95%CI=0.098to0.99; p=0.017) and maternal employment status (b=0.08; 95%CI=-0.29to0.45; p=0.683).Conclusion: The direct determinants of maternal death include late decision making, late transfer to the hospital, late handling at the hospital, and complication. The indirect determinants of maternal death include completeness of antenatal visits, existence of pregnancy risk factor, maternal education, and maternal employment status.Keywords: determinant, delay, complication, antenatal care, maternal death Correspondence: Ratna Diana Fransiska. Masters Program in Public Health, Sebelas Maret University. Email: ratnadiana00@gmail.com. Mobile: 085778822668Journal of Maternal and Child Health (2017), 2(1): 76-88https://doi.org/10.26911/thejmch.2017.02.01.08