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Potensi Alang-alang (Imperata cylindrica (L.) Beauv) dalam Produksi Etanol Menggunakan Bakteri Zymomonas mobilis Kartikasari, Sevy Dwi; Nurhatika, Sri; Muhibuddin, Anton
Jurnal Sains dan Seni ITS Vol 2, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (769.825 KB) | DOI: 10.12962/j23373520.v2i2.3741

Abstract

Sumber selulosa yang murah dan melimpah dapat diperoleh dari gulma alang-alang (Imperata cylindrica (L.) Beauv). Produksi etanol dari bahan baku selulosa alang-alang umumnya difermentasikan oleh yeast. Pada penelitian ini fermentasi dilakukan menggunakan bakteri Zymomonas mobilis. Penelitian dilakukan pada bulan November 2012 hingga Mei 2013 di laboratorium Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITS. Penelitian bertujuan untuk mengetahui potensi alang-alang (I. cylindrica (L) Beauv.) dalam produksi etanol menggunakan bakteri Z. mobilis pada konsentrasi inokulum dan waktu fermentasi yang optimum. Penelitian ini dilakukan dalam tiga langkah utama yaitu pretreatment substrat, hidrolisis enzimatik oleh Penicillium sp., dan fermentasi etanol oleh Z. mobilis. Fermentasi dilakukan menggunakan berbagai perlakuan konsentrasi inokulum (0, 5, 10, dan 15%) dan lama fermentasi (0, 3, 5, 7, dan 9 days) serta dianalisa menggunakan uji Analysis of Varians (ANOVA). Masing-masing perlakuan dilakuan 2 kali pengulangan, sehingga diperoleh 40 unit percobaan dengan parameter yang diamati adalah kadar etanol. Hasil penelitian menunjukkan bahwa alang-alang (I. cylindrica (L.) Beauv.) berpotensi untuk dijadikan sebagai bahan baku produksi etanol dengan konsentrasi etanol tertinggi diperoleh dari interaksi antara 10% konsentrasi inokulum Z. mobilis dan waktu fermentasi 7 hari yaitu sebesar 9,02 % (v/v)
Correlation between Soil Phospor (P) Content and Soil Yeast Antagonistics Ability Against Fusarium oxysporum Pathogen Causing Wilt Muhibuddin, Anton; Wibowo, Kartika Novitasari; Punnapayak, Hunsa; Goetz, Peter
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.1

Abstract

Besides its role during fermentation process, yeast also has potential as biological agent because of its antagonistics characteristic. We have isolated yeast from six different locations based on Phospourus differences. Isolated yeast were then tested its antagonistics ability of Fusarium oxysporum, the pathogen causing tomato wilt disease. This research aims to find out the diversity of yeast found in the tomato rhizosphere in six different contents of phosphorus locations and to know its antagonistics ability against the pathogenic. F. oxysporum. This research was conducted in the Laboratory of Mycology, Department of Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang and in the Chemistry Laboratory, Institut Teknologi 10 Nopember Surabaya, started from January up to September 2015. Yeasts have been isolated from tomato’s rhizosphere of 6 different locations around East Java proviences. The result showed that yeasts from organic field (Lower P content) were 6 genera. They are Candida sp. 1, Pichia sp. 1, Hansenula sp., Metschnikowia sp. 1, Cryptococcus sp., and Zygosaccharomyces sp. While the yeasts from inorganic field (higher P) were 3 genera. The most potential yeast in controlling F. oxysporum is Pichia sp. 2. Lower P content showed more divers than higher P content. Yeasts from higher soil P content showed more antagonists to control F. oxysporum. Keywords: Phosphor, correlation, yeast, antagonistic, disease, soil
DECREASE CLUBROOT DISEASE INTENSITY OF Brassica juncea ON Pb CONTAMINATED SOIL USING Paraserianthes falcataria THAT WAS INFECTED BY Mycorrhizal Kurniawan, E; Muhibuddin, Anton; Kusuma, R R
Journal of Environmental Engineering and Sustainable Technology Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.972 KB) | DOI: 10.21776/ub.jeest.2016.003.01.8

Abstract

Both, biotic and abiotic factor is the most important problem for agriculture in Indonesia. Biotic factor such as plant disease could decrease yield till more than 50%, while abiotic factor such as soil contamination could decrease yield till more than 30%. One of choice to overcome this problem is using bio-phytoremediation method. This method  combine remediation using plant (phytoremediation) and remediation using microbe (bio remediation). This study was aimed to determine the effects of bio-phytotemediation method using Paraserianthes falcataria which infected by mycorrhizal fungi to overcome Phytophthora brassicae, the main clubroot disease on Brassica juncea, in Pb contaminated soil. We also aimed to know the influence of the method to absorb soil Pb using Paraserianthes falcataria. The research was conducted in the Mycology Faculty of Agriculture, University of Brawijaya from March to October 2015. The research used Completely Randomized Design with 6 treatments and 4 replications. Parameter observation in this research were: 1) Intensity of clubroot disease; 2) Growth of B. juncea; 3) Population of mycorrhizal spores in soil,  4) Mycorrhizal infection inside plant cell; and 4) Pb content of soil and plant tissues. The results was showed  that mycorrhizal has significantly effects to decrease clubroot disease intensity. But, mycorrhizal has no significantly effect to the plant growth, including stem length and leaves number. The number of mycorrhizal spores in the soil after 35 days application was increases, and the percentage of infection in the roots of B. juncea and P. falcataria was fluctuated. The application of mycorrhizal can decrease Pb content in the soil and increase Pb content in P. falcataria.
ETHANOL FERMENTATION POTENCY OF WILD YEAST ON BAMBOO RHYZOSPHERE Muhibuddin, Anton
Journal of Environmental Engineering and Sustainable Technology Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.442 KB) | DOI: 10.21776/ub.jeest.2017.003.02.8

Abstract

Bamboo plant rhizosphere known as source of simbiotic useful microorganisms, including yeast. Wild yeast explored should be tested it’s adaptability to new ecology especially nutritional source availability. The research aim to get potential yeast which can work well during fermentation process in apple juice substrate.We were isolated yeast from three different locations: Ketawanggede District, Karangploso District, and Lowokwaru District. All locations was located around Malang city.Result showed that there were found 13 isolates yeast: Protomyces sp, Agaricostilbum sp1, Agaricostilbum sp2, Agaricostilbum sp3, Debaryomyces sp1, Debaryomyces sp2, Debaryomyces sp3,  Trigonopsis sp1, Trigonopsis sp2, Udeniomyces sp1, Udeniomyces sp2, Ascoidea hylocieti, and Komagataella sp. Diversity index indicates medium category to low category and dominance index in all location indicates high category.Fermentation test showed improvements in observation variables at 24 and 72 hours including temperature, cells number, and alcohol percentage. The highest alcohol percentage were 11.6% and 10% that produced by the treatment of Agaricostilbum sp3 and Trigonopsis sp1 respectively.Keyword: Yeast, bamboo plant rhizosphere, apple juice, and fermentation.
Yeast On Epiphyte of Tangerines Citrus Fruit (Citrus nobilis L.) and Their Potential Antagonist to Colletotrichum Gloeosporioides (Penz.) Penz. & Sacc. Pramudita, Oki; Muhibuddin, Anton
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.3

Abstract

One of the most important diseases of citrus plant is anthracnose which caused by Colletotrichum gloeosporioides (Penz.) fungi. Yeast is one of microbes which has a good potential to control the disease. This research focused on exploring yeasts on tangerines and examining their efficacy to control C. gloeosporioides using in vitro and in vivo treatments. Observation was started by isolated C. gloeosporioides and yeast from tangerines citrus fruit, then followed with in vitro and in vitro treatment. Both were counted the percentage of antagonist and pathogen incubation stage and also disease incidence, respectively. Result showed that yeast has been isolated from tangerines fruit were Candida sp. (isolate 1), Candida sp. (isolate 2) and Pichia sp. All yeast showed inhibit C. gloeosporioides growth and suppressed its development.
SOIL DRIVE NUTRIENT AS NEW METHOD FOR TIN MINING REMEDIATION Muhibuddin, Anton
Journal of Environmental Engineering and Sustainable Technology Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1270.918 KB)

Abstract

A contaminated soil by tin usually is a big problem in Indonesia. Uniformity is essential for processing soil at a normal quality and to ensure conformity to specify clean up levels. Revegetation efforts were carried out by using rubber and jatropha combination which infected by arbuscular mycorrhizal (VAM) fungi applied using the soil drive nutrient (SDN) method showed good result in the previously research.This research purposes to know the effectivity of soil drive nutrient (SDN) method to promote the growth of soybean plants (Glysine max L.) in tin mining soil. The results showed that the cropping pattern and dose of mycorrhizal gave effect on soybean growth, especially on the trunk diameter. Result also showed that mycorrhizal infection on soybean roots significantly influenced by the SDN method and dose of mycorrhizal that applied. Both of these are expected to have a positive impact on soybean production and resistance to environmental stress and disease attack. Viceversa, height and number of plant leaves untill 28 days were not affected by the SDN method and the dose of mycorrhiza applied.
Potential of Wild Yeast from Banana to Control Colletotrichum musae Fungi Caused Anthracnose Disease and Its Short Antagonistic Mechanism Assay Muhibuddin, Anton; Sektiono, Antok Wahyu; Sholihah, Dewi Maratus
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.745 KB)

Abstract

Anthracnose caused by Colletotrichum musae is one of several important disease in banana fruit. An effective, cheap, and safe control method are necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that has antagonist ability effectively in controlling anthracnose disease of banana fruit.  This research was started with an isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from ambon variety was Candida sp. and Pichia sp. Metchnikowia sp. from kepok variety respectively.
THE ROLE OF INDIGENOUS MYCORRHIZA IN COMBINATION WITH CATTLE MANURE IN IMPROVING MAIZE YIELD (ZEA MAYS L) ON SANDY LOAM OF NORTHERN LOMBOK, EASTERN OF INDONESIA Astiko, Wahyu; Sastrahidayat, Ika Rochdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
Journal of Tropical Soils Vol 18, No 1: January 2013
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2013.v18i1.53-58

Abstract

A glass house study was conducted to evaluate the contribution of indigenous arbuscular mycorrhiza fungi (AMF) in improving maize yield grown on sandy loam of Northern Lombok. The package of organic fertilizers treatments were tested including: without inoculation of mycorrhiza, inoculation mycorrhiza and no added inorganic fertilizers, inoculation of mycorrhiza with cattle manure added, inoculation of mycorrhiza with rock phosphate added and inoculation mycorrhiza with inorganic fertilizers. The treatments were arranged using a Completely Randomized Design with four replications. The results of the study show that the inoculation of AMF significantly increased soil concentration of N, available-P, K and organic-C by 37.39%, 60.79%, 66.66% and 110.15% respectively observed at 60 days after sowing (DAS). The similar trend was also found at 100 DAS, where those nutrients increased by 21.48%, 69%, 43.93% and 37.07%, respectively compared to control. The improving of soil fertility status was also reflected by nutrients uptake (i.e. N, P, K, Ca) as well as growth and yield of maize. N, P, K and Ca uptake increased by 1,608%, 1,121%, 533% and 534%, respectively. Roots and top dry biomass at 60 DAS increased by 718.40% and 337.67%, respectively. The trend increased of the biomass was followed by observation at 100 DAS. Yield components including cobs, grain and weight of 100 grains increased by 313.60%, 411.84% and 137.54%, respectively. In addition, the inoculation of AM with F2 contributed significantly to the spore numbers and root infection.[How to Cite : Astiko W, IR Sastrahidayat, S Djauhari, and A Muhibuddin. 2013. The Role of Indigenous Mycorrhiza in Combination with Cattle Manure in Improving Maize Yield (Zea Mays L) on Sandy Loam of Northern Lombok, Eastern of Indonesia. J Trop Soils, 18 (1): 53-58. doi: 10.5400/jts.2013.18.1.53][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.53]
Endophyte Fungi to Control Helminthosporium turcicum, Fungi Causing Leaf Blight Disease Sastrahidayat, Ika Rochdjatun; Faizah, Akhmat Riza; Muhibuddin, Anton
SAINTEKBU Vol 10 No 1 (2018)
Publisher : KH.A.Wahab Hasbullah University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5334.942 KB)

Abstract

Leaf blight disease is one of the most dangerous diseases of maize plant. This disease be able causing loss of maize harvest up to 40-70%. The cause of the leaf blight disease is Helminthosporium turcicum fungi. Biological control with endophytic fungi potentially used to control the pathogens as well as increase the resistance of plant induction to disease. Research was conducted in PT Bisi Inernational area, Tbk Farm Ngantang Malang Regency, Laboratory of Mycology Department of Plant Pest and Disease, Faculty of Agriculture Brawijaya University and Bioscience Laboratory Brawijaya University on December 2016 until July 2017. The exploration result of endophytic fungi of three varieties of maize were obtained 30 isolates, consisting of 14 non-pathogenic isolates and 16 pathogenic isolates. Based on the power inhibition percentage to the highest growth of H. turcicum is a fungi with NuII2, NuII4, PuIII3, DuIII3, NuII3 and PuIII1 isolates code. The most prevalent inhibition mechanism is pathogens and endophytic fungi competition. The 30 isolates identified were Colletotrichum sp, Fusarium sp, Curvularia sp, Acremonium sp dan Paecilomyces sp, meanwhile the other endophytic fungi is only found sterile hyphae thus difficult to identify up to the genus level. Keywords : Endophyte fungi, Zea mays, Helminthosporium turcicum  
Fungi in Rice Straw, Cane Straw, Maize Straw and Their Potential as Decomposer Sastrahidayat, Ika Rochdjatun; Situmorang, Chintya Ivana; Muhibuddin, Anton
SAINTEKBU Vol 10 No 1 (2018)
Publisher : KH.A.Wahab Hasbullah University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8431.763 KB)

Abstract

Organic material decomposition is the reorganizing process of the organic material by microbes in the controlled circumstances. The microbes which were used in general such as fungi, bacteria or yeast. Aerobic yeasts is one of the microbes needs oxygen to work. Yeast was obtained by the exploration of the rice straw, maize straw and cane straw in Dau, Malang, East of Java. By the result of the microscopic exploration and observation through the microscope, it was obtained 9 yeast isolates and 3 fungi isolates. 2 yeast isolates and 1 fungi isolate from the rice straw, 2 yeast isolates and 1 fungi isolate from the maize straw, and 5 yeast isolates and 1 fungi isolate from the cane straw. The identification results were obtained Candida parapsilosis, Bellera oryzae, Kluyveromyces thermotolerant, Candida tropicalis, Debaryomyces hansenii, Wickerhamomyces anomalus, Pichia membranfaciens, Cryptococcus wieringae. The highest potential yeast as an organic fertilizer decomposer is Cryptococcus wieringae and the lowest is Bullera oryzae. Keywords : Candida, Pichia, Cryptococcus, Bullera, Kluyveromyces, DebaryomycesWickerhamomyces, Fusarium and Trichoderma