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PENGARUH ASUPAN AIR PUTIH TERHADAP BERAT BADAN, INDEKS MASSA TUBUH, DAN PERSEN LEMAK TUBUH PADA REMAJA PUTRI YANG MENGALAMI GIZI LEBIH Mulyasari, Indri; Muis, Siti Fatimah; Kartini, Apoina
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 3, No 2 (2015)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.043 KB) | DOI: 10.14710/jgi.3.2.120-125

Abstract

Latar belakang : Remaja putri yang kelebihan berat badan dengan indeks massa tubuh (IMT) dan persen lemak tubuh tinggi berisiko menderita sindrom metabolik. Beberapa penelitian melaporkan peningkatan asupan air putih dapat menurunkan berat badan, IMT, dan persen lemak tubuh. Tujuan : Mengetahui pengaruh asupan air putih terhadap berat badan, IMT, dan persen lemak tubuh pada remaja putri yang mengalami gizi lebih.Metode : Desain penelitian adalah pre-post test design without control. Populasi adalah mahasiswa di Asrama Kebidanan Ngudi Waluyo Ungaran usia 18-19 tahun. Jumlah subjek 26 mahasiswa dipilih secara simple random sampling sesuai kriteria inklusi. Perlakuan adalah asupan air putih 30 menit sebelum makan sebanyak 500 ml selama delapan minggu, namun hanya berjalan lima minggu karena penolakan subjek. Tidak ada intervensi pada asupan makanan dan aktivitas fisik. Berat badan diukur menggunakan timbangan injak digital Omron dan persen lemak tubuh diukur dengan Bioelectrical Impedance Analyzer (BIA) merk Omron. Data dianalisis menggunakan uji Paired t test dan Wilcoxon.Hasil : Rata-rata asupan air putih, yaitu 90.82 % (±454 ml) setiap kali sebelum makan. Anjuran mengkonsumsi 500 ml air putih 30 menit sebelum makan selama 8 minggu sulit dilaksanakan sehingga perlakuan hanya berlangsung lima minggu. Tidak ada perbedaan berat badan (62.9±6.75 kg vs 62.5±6.73 kg, p=0.066) dan IMT (26.6±2.69 kg/m2 vs 26.4±2.71 kg/m2, p=0.071) sebelum dan sesudah perlakuan, namun terdapat perbedaan pada persen lemak tubuh (34.2±2.76 % vs 33.7±3.05 %, p=0.037). Tingkat asupan energi (p=0.713) dan aktivitas fisik sebelum dan sesudah perlakuan tidak mengalami perubahan.Simpulan : Asupan air putih 30 menit sebelum makan sebanyak 454 ml (90.82%) selama lima minggu pada remaja putri dengan gizi lebih yang tidak mengalami perubahan asupan energi dan aktivitas fisik tidak menurunkan berat badan dan IMT, namun menurunkan persen lemak tubuh.
PENGARUH SUPLEMENTASI ZN TERHADAP PERUBAHAN INDEKS TB/U ANAK STUNTED USIA 24-36 BULAN Kusudaryati, Dewi Pertiwi Dyah; Muis, Siti Fatimah; Widajanti, Laksmi
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 2 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.177 KB) | DOI: 10.14710/jgi.5.2.98-104

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Background : Zn supplementation on stunted children in multiple research get the result inconsistent concerning the effect of Zn on children?s growth.Objective : To examine the effect of Zn supplementation to the change in height for index among stunted children age between 24-36 months.Methods: The research design is randomized pretest posttest control group design. The total of thirty six stunted children are divided into two groups. The treatment group is received syrup with 20 mg ZnSO4 twice a week for three months. The control group received placebo without Zn. Height for age z score (HAZ) on WHO Child Growth 2006 is used to measure height for age index while dietary intake and infection diseases are used as confounding variables.Result : There is significant difference in HAZ before and after supplementation at treatment group (p<0.001) and control group (p<0.001). There is a significant difference of the change in HAZ between the treatment group and control group (p=0.006). Percentage Achievement of Energy, Protein, and Zn Dietary Allowance have significant difference between the treatment group and control group (p=0.009; p<0.001; p<0.001, respectively). The change in HAZ, Percentage Achievement of Energy, Protein, and Zn Dietary Allowance are higher in treatment group than control group. Duration of diarrhea (p=0.045) and morbidity (p=0.019) are lower in treatment group than control group. Conclusion : Zn Supplementation among stunted children have significant effect on the change in HAZ, dietary intake, and infection.
KANDUNGAN ISOVLAVON DAN VITAMIN E PADA FORMULA KOMBINASI TEPUNG TEMPE DAN BEKATUL UNTUK MEMPERBAIKI PROFIL LIPID DARAH Bintanah, Sufiati; Muis, Siti Fatimah; AP, Purwanto
Jurnal Gizi Vol 7, No 2 (2018): Jurnal Gizi UNIMUS
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1210.139 KB) | DOI: 10.26714/jg.7.2.2018.%p

Abstract

The prevalence of cardiovascular disease continues to increase as a result of dietary changes that have an impact on abnormal lipid profiles and oxidative stress. Efforts to improve lipid profiles byconsuming food contain antioxidant compounds that have the potential to improve blood lipid profiles. The aim of the study was to obtain a description of the content as an antioxidant in the formula of the combination of soybean tempeh flour with rice bran.Research method: This type of experimental research is to find out the most optimum antioxidant content of the formula. Making formulas, (1) yellow soybean tempeh flour + white bran flour, (2 black soybean tempeh flour + white bran flour (3) yellow soybean flour tempeh + red bran flour,(4) black soybean flour tempeh + red bran flour, with composition of 1: 1 and weighs 112 grams each, non-calorie sweetener 3 grams and food flavorings 3 grams, dissolved in cold water as muchas 250 cc, analysis of nutrients using the AOAC method, 2005 . Data analysis: Data obtained are averaged and displayed in table form, then compared between treatments. Results: The combination of YST + RBF with a composition of 1: 1 has a higher isovlavone content while the highest content of vitamin E is found in the combination of YST + WBF. Conclusion: There is sufficient isovlavone and tokoverol content from a combination of tempeh flour and bran to improve blood lipid profile.Keywords: Isoflavones, vitamin E, formula of tempeh and bran flour, blood lipid profile
KONSELING LAKTASI INTENSIF DAN PEMBERIAN AIR SUSU IBU (ASI) EKSKLUSIF SAMPAI 3 BULAN Ambarwati, Ria; Muis, Siti Fatimah; Susantini, Purwanti
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 3 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Intensive lactation counseling and exclusive breastfeeding up to 3 monthsBackground: In 2010 within the area of Semarang Municipality, exclusive breastfeeding by mothers to their babies reaches up to only 20,06%. One of the problems is the lack of lactation counseling especially by the health service institutions.Objectives: To reveal the effect of intensive lactation counseling on exclusive breastfeeding up to 3 months.Method: This research study is designed as a quasi-experiment of non-equivalent control group. The population of this study is a group of mothers who were in the 7th to 8th months of pregnancy bearing the second child or more. The number of the subjects is 25 mothers who were grouped into 2, that is the experimental groups of 12(n=12) and control group of 13 (n=13). The group underwent a treatment of intensive lactation counseling of 9 times on 2 stages, the first was four-time treatments during the medical examination on their pregnancy of 7th-8th months. The second stage of treatments was done by home visit for 5 times during week 1, 2, 4, 8, and 12 after giving birth by sertified lactation counselors for 40 workhours. These counselors had the experience of at least 5 clients counseling. The control group got the usual counseling that they normally received from the midwives at the Health Primary Center and private midwives nearby.Results: The group without intensive lactation counseling shows no gain of knowledge, attitude towards early initiation of breastfeeding, exclusive breastfeeding, breastmilk and no increase of the number of exclusive breastfeeding on their babies prior to and during the study. The group with intensive lactation breastfeeding shows a significant gain in every aspects being studied and significant increase (five fold) of the number of exclusive breastfeeding on their babies that born prior to and during the study from2(16.7%) to 10(83.3%).Conclusion: Intensive lactation counseling increases the practice of exclusive breastfeeding up to 3 months and should be performed by health care delivery system.Keywords: Intensive lactation counseling, exclusive breastfeeding, 3 months ABSTRAKLatar belakang: Ibu yang memberikan ASI eksklusif di Kota Semarang Tahun 2010 hanya mencapai 20,06%. Salah satu penyebabnya adalah kurangnya konseling laktasi di sarana pelayanan kesehatan.Tujuan: Mengetahui pengaruh konseling laktasi intensif terhadap pemberian ASI eksklusif sampai 3 bulan.Metode: Penelitian dengan rancangan quasi experiment non equivalent control group. Populasi adalah ibu hamil trimester 3 dengan usia kehamilan 7-8 bulan pada anak kedua atau lebih. Jumlah sampel 25 ibu hamil yang dikelompokkan menjadi 2 yaitu kelompok perlakuan (n=12) dan kelompok kontrol (n=13). Kelompok perlakuan mendapat konseling laktasi intensif sebanyak 9 kali yaitu 4 kali pada saat pemeriksaan kehamilan umur 7-8 bulan dan kunjungan rumah sebanyak 5 kali pada minggu ke-1, 2, 4, 8, 12 setelah kelahiran oleh konselor laktasi yang memiliki sertifikat pelatihan konselor laktasi. Kelompok kontrol mendapat konseling pada pemeriksaan kehamilan yang selama ini dilakukan oleh bidan di Puskesmas dan Bidan Praktik Swasta.Hasil: Pada kelompok yang tidak mendapat konseling laktasi intensif tidak ada perubahan skor pengetahuan, sikap terhadap inisiasi menyusu dini, ASI eksklusif, ASI, menyusui dan tidak ada peningkatan jumlah ibu yang memberikan ASI eksklusif sampai 3 bulan pada anak yang dilahirkan sebelum dan selama penelitian. Kelompok yang mendapat konseling laktasi yang intensif menunjukkan ada perubahan skor pada semua anak dan peningkatan jumlah ibu yang memberikan ASI eksklusif sampai 3 bulan pada anak yang dilahirkan selama penelitian menjadi 5 kali lipat.Simpulan: Konseling laktasi intensif dapat meningkatkan jumlah ibu yang melaksanakan ASI eksklusif sampai 3 bulan dan perlu dilaksanakan oleh semua sarana pelayanan persalinan.
PRAKTIK INISIASI MENYUSU DINI DAN PEMBERIAN ASI EKSKLUSIF STUDI KUALITATIF PADA DUA PUSKESMAS, KOTA SEMARANG Noer, Etika Ratna; Muis, Siti Fatimah; Aruben, Roni
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTEarly initiation of breastfeeding and exclusive breastfeeding practice: qualitative study at public health centers, Semarang cityBackground: Early initiation of breastfeeding (EIB) and exclusive breastfeeding (EB) up to six months are two important breastfeeding practices for child?s survival. EIB plays an important role in reducing infant mortality and improve the success of exclusive breastfeeding. Determinants in EIB and EB practices are predisposing, enabling, reinforcing, and environment. This study aimed to investigate determinants in EIB and EB practice until 4 months.Method: This study used a qualitative approach. Data were collected through intensive observations, non-intervention and in-depth interviews to 12 mothers. Data were analyzed using content analysis including data collection, reduction, presentation, and conclusions.Result: All the mothers were successful in the EIB with the help of professional midwife. Exclusive breastfeeding were successful in 3 mothers, partial breastfeeding were practiced by 6 mothers and not breastfeeding or formula feeding were practiced by 3 mothers. Predisposing and reinforcing were two strongly factors that cause the unsucessfull EB practices. The former were lack of knowledge of EB and breastfeeding techniques. The latter were lack of commitment of health providers, family support, and the status ofworking mothers.Conclusion: EIB practice does not ensure the success of EB.Keywords: Early initiation of breastfeeding, exclusive breastfeeding, qualitativeABSTRAKLatar belakang: Inisiasi menyusu dini (IMD) dan ASI eksklusif sejak lahir hingga usia enam bulan merupakan dua praktik pemberian ASI yang penting untuk kelangsungan hidup dan pertumbuhan optimal bayi. IMD berperan penting dalam mengurangi angka kematian bayi dan meningkatkan keberhasilan pemberian ASI eksklusif. Beberapa faktor determinan dalam praktik IMD dan  ASI eksklusif yaitu faktor predisposisi (predisposing), pemungkin (enabling), penguat (reinforcing), dan lingkungan. Penelitian inibertujuan untuk mempelajari beberapa faktor determinan pada praktik IMD dan ASI eksklusif selama 4 bulan pada ibu yang melahirkan pada dua puskesmas di kota Semarang. Hasil penelitian ini akan diteruskan sebagai masukan terhadap program peningkatan cakupan pemberian ASI eksklusif.Metode: Penelitian ini menggunakan pendekatan kualitatif pada 12 ibu. Pengumpulan data dilakukan dengan observasi intensif, non intervensi dan menggunakan teknik wawancara mendalam. Data dianalisis dengan cara analisis isi yang bersifat terbuka. Hasil: Semua ibu dapat melakukan praktik IMD dengan bantuan profesionalisme bidan puskesmas. Praktik ASI eksklusif dilaksanakan oleh 3 ibu, ASI parsial pada 6 ibu dan pemberian susu formula oleh 3 ibu. Hambatan utama praktik pemberian ASI eksklusif adalah dari faktor ibu yaitu rendahnya pengetahuan tentang ASI eksklusif dan teknik menyusui. Faktor penghambat lainnya adalah kurangnya komitmen petugas kesehatan, dukungan suami, dan status ibu bekerja.Simpulan: Praktik IMD tidak menjamin keberhasilan pemberian ASI eksklusif.
SUMBANGAN ALL-TRANS ASAM RETINOAT (ATRA) BAGI PENYEMBUHAN PERIODONTITIS Praptiwi, Praptiwi; Muis, Siti Fatimah; Hadisaputro, Soeharyo; Suryono, Suryono
MEDIA MEDIKA INDONESIANA 2011:MMI Volume 45 Issue 3 Year 2011
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACTAll-trans retinoic acid (ATRA) contribution to periodontitis healingBackground: In inflammation and infection of periodontal ligament i.e periodontitis, collagen fiber is damaged. All-trans retinoic acid (ATRA), metabolite of vitamin A, has potency to depress damage in inflammation. Indonesia with high vitamin A deficiency, facing to delayed recovery of periodontitis. The disease is vastly suffered by common people in this country. The study aimed to know the collagen status of periodontal ligament suffering periodontitis treated with tetracycline and ATRA supplementation.Method: The experimental study using post test only design in 12 adult male Wistar rat was done in 7 days. The animal study were induced periodontitis through Porphyromonas gingivalis inoculation. Tetracycline 90 mg/kg was given, beside ATRA supplementation of 10 mg/kg and 5 mg/kg. Four experimental groups were chosen randomly. Group of periodontitis + tetracycline was the control of the study. The independent variables were ATRA supplementation of those high and low dose. The dependent variable was collagen status. Collagen status consist of degradation and synthesis of collagen, indicated by mRNA MMP-2 and mRNA P1CP respectively. They were measured by reversed transcriptase polymerizing chain reaction and thin layer chromatography. All-trans retinoic acid concentration was measured using high performance liquid chromatography. The dataanalyzed using t-test, Oneway ANOVA, post hoc duncan and least significant differences.Result: In group receiving 10 mg ATRA/kgBB, the ATRA level was highest but the collagen degradation was lower although not significant compared to the low dose ATRA group. In both groups the degradation was significantly lower than the control group (p=0.007). The synthesis of collagen in the low dose ATRA group was higher than the high dose ATRA group and the control group.Conclusion: High dose of ATRA supplementation contribution to less degradation of collagen, but in term of collagen synthesis, low dose ATRA supplementation gives better result.Keywords: ATRA supplementation, collagen degradation, periodontitis ABSTRAKLatar belakang: Pada peradangan dan infeksi kronik ligamentum periodontal yaitu periodontitis, terjadi kerusakan serat kolagen. All-trans asam retinoat (ATRA), metabolit vitamin A, berpotensi menekan kerusakan pada peradangan. Tingginya defisiensi vitamin A di Indonesia, menghambat proses penyembuhan periodontitis yang banyak diderita masyarakat luas. Penelitian bertujuan mengetahui status kolagen ligamentum periodontal periodontitis yang mendapat pengobatan tetrasiklin dan suplementasi ATRA.Metode: Studi eksperimental dengan rancangan post test only pada 12 tikus Wistar jantan dewasa telah dilakukan selama 7 hari. Hewan coba diinduksi periodontitis melalui inokulasi bakteri Porphyromonas gingivalis. Diberikan tetrasiklin 90 mg/kg, juga suplementasi ATRA 10 mg/kg dan 5 mg/kg. Empat kelompok studi dipilih secara acak. Kontrol adalah kelompok sakit + tetrasiklin. Variabel bebas adalah suplementasi ATRA dosis tinggi dan dosis rendah, variabel tergantung adalah status kolagen. Status kolagenmencakup degradasi dan sintesis, berturut-turut dengan petanda mRNA MMP-2 dan mRNA P1CP, diukur menggunakan reverse transcriptase polymerizing chain reaction dan thin layer chromatography. Kadar ATRA diukur dengan high performance liquid chromatography. Data dianalisis dengan uji t, Oneway ANOVA, post hoc Duncan dan least significant differences.Hasil: Pada kelompok yang menerima suplementasi ATRA 10 mg/kgBB kadar ATRA tertinggi, tetapi degradasi kolagennya lebih rendah walau tidak bermakna. Kedua kelompok sumplementasi degradasi kolagennya lebih rendah secara bermakna dibanding kelompok kontrol (p=0,007), dalam sintesis kolagen,kelompok yang mendapat suplementasi ATRA 50 mg/kgBB sintesisnya lebih baik dibanding kelompok yang mendapat ATRA 10 mg/kgBB maupun kelompok kontrol.
HUBUNGAN ANTARA LINGKAR PINGGANG DENGAN KOMPOSISI LEMAK TUBUH DAN INDEKS MASSA TUBUH: STUDI PADA PENDERITA DIABETES MELITUS TIPE 2 Nurhasanah, Nurhasanah; Muis, Siti Fatimah; Murbawani, Etisa Adi; Probosari, Enny; Nugroho, Heri
JNH (Journal of Nutrition and Health) Vol 7, No 3 (2019): JNH (JOURNAL OF NUTRITION AND HEALTH)
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.491 KB) | DOI: 10.14710/jnh.7.3.2019.43-49

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ABSTRAKLatar belakang: Obesitas merupakan faktor risiko mayor terjadinya diabetes melitus (DM), berhubungan dengan penumpukan jaringan lemak tubuh. Banyak metode untuk mengukur jaringan lemak diantaranya indeks massa tubuh (IMT), lingkar pinggang (LP) dan Bioelectrical impedance analysis (BIA). Distribusi lemak tubuh lebih berperan dalam menentukan risiko terjadinya DM dibandingkan IMT.Tujuan: untuk mengetahui hubungan LP dengan komposisi lemak tubuh dan IMT pada penderita DM tipe 2.Metode: Penelitian cross sectional pada penderita DM tipe 2 yang berobat ke RSUD Tugu Semarang bulan September 2018-Maret 2019, n= 80 orang, dengan consecutive sampling. Uji normalitas data dengan Kolmogorov-Smirnov, jika data normal dilanjutkan uji korelasi Pearson dan jika data tidak normal dilanjutkan uji korelasi Spearman?s rho.  Hasil: LP memiliki hubungan signifikan, positif dan moderat dengan persentase total lemak tubuh (p <0,001, r = 0,451), hubungan signifikan, positif dan kuat dengan persentase lemak viseral (p <0,001, r= 0,693) dan lemak subkutan (p <0,001, r = 0,518). LP memiliki hubungan signifikan, positif dan sangat kuat dengan IMT (p <0,001, r = 0,801).Simpulan: LP memiliki hubungan positif kuat dengan lemak viseral, lemak subkutan dan IMT pada penderita DM tipe 2.Kata Kunci: lingkar pinggang, komposisi lemak tubuh, IMT, DM tipe 2
INTERVENSI NON FARMAKOLOGI TERHADAP KECEMASAN PADA PRIMIGRAVIDA Susilowati, Tri; Pramana, Noor; Muis, Siti Fatimah
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 9 No 3 (2019): Juli
Publisher : LPPM STIKES KENDAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.325 KB) | DOI: 10.32583/pskm.9.3.2019.181-186

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Kehamilan merupakan suatu krisis maturitas yang dapat menimbulkan kecemasan. Calon ibu yang belum pernah melahirkan sebelumnya atau yang disebut primigravida lebih banyak mengalami kecemasan dibandingkan ibu yang pernah melahirkan atau yang disebut multigravida. perlu dilakukan upaya untuk meminimalkan kecemasan yang terjadi selama masa kehamilan baik melalui cara farmakologis maupun non farmakologis. Literature review ini bertujuan untuk mengetahui intervensi yang digunakan untuk mengurangi kecemasan pada primigravida yang sudah dilakukan oleh peneliti sebelumnya. Metode penulisan karya ilmiah ini menggunakan studi literature review. Sumber pustaka menggunakan artikel dengan proses pencarian artikel data base dan Jurnal tahun penerbitan 2015 hingga tahun 2019. Tema dalam artikel yang dikumpuklan yaitu terkait kecemasan pada ibu hamil. Kecemasan yang terjadi dipengaruhi dengan karakteristik masing-masing individu. Kecemasan ibu hamil mampu dicegah atau diturunkan melalui terapi kelompok suportif, terapi relaksasi, relaksasi otot progresif, relaksasi gim (guided imagery and music) dan aromaterapi lavender, teknik pernapasan diafragma, terapi musik klasik, senam hamil, terapi murottal al qur?an, SEFT (spiritual emotional freedom technique) dan terapi benson. &nbsp; Kata kunci: kecemasan, primigravida &nbsp; NON-PHARMACOLOGICAL INTERVENTION ON ANXIETY IN PRIMIGRAVIDA &nbsp; ABSTRACT Pregnancy is a crisis of maturity that can cause anxiety. Prospective mothers who have never given birth before or who are called primigravida experience more anxiety than mothers who have given birth or are called multigravida. efforts need to be made to minimize the anxiety that occurs during pregnancy both through pharmacological and non-pharmacological methods. This literature review aims to determine which interventions are used to reduce primigravida anxiety that has been done by previous researchers. This method of writing scientific papers uses the literature review study. Literature sources use articles with a 2015 data base search journal and publishing year journal article until 2019. Themes in articles collected are related to anxiety in pregnant women. Anxiety that occurs is influenced by the characteristics of each individual. Anxiety of pregnant women can be prevented or reduced through supportive group therapy, relaxation therapy, progressive muscle relaxation, guided imagery and music and lavender aromatherapy, diaphragmatic breathing techniques, classical music therapy, pregnancy exercises, murottal al qur'an therapy, SEFT (spiritual emotional freedom technique) and benson therapy. &nbsp; Keywords: anxiety, primigravida
NILAI GIZI, DAYA CERNA PROTEIN DAN DAYA TERIMA PATILO SEBAGAI MAKANAN JAJANAN YANG DIPERKAYA DENGAN HIDROLISAT PROTEIN IKAN MUJAIR (OREOCHROMIS MOSSAMBICUS) Haslina, Haslina; Muis, Siti Fatimah; Suyatno, Suyatno
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Volume 1. Nomor 2. Juni 2006
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.52 KB) | DOI: 10.14710/jgi.1.2.

Abstract

ABSTRACT Introduction: Patilo is one of traditional various snacks from Gunung Kidul that is made of fermented cassava waste and cassava starch. Fermentation is intended to reduce or eliminate the HCN content of cassava and to establish special flavour. The protein content of patilo is very low, therefore it can be enriched with animal protein from fresh water fish in form of fish flour or fish protein hydrolysate (FPH). Enrichment with fish protein hydrolysate has an advantage since it will be easier to digest by human because FPH better functional properties and higher solubility.Method: The study consisted of four activities that were 1) making/producing mujair FPH, 2) producing and enriched patilo with mujair FPH, 3) analyzed the nutritive values and protein digestibility of enriched and unenriched patilo and 4) analyzed the consumers acceptability of the products. The experiments were carried out in the 1) Food Engineering and Chemistry Laboratorium of Faculty of Agriculture Technology Semarang University, 2) Pilot Plan PAU and GMSK Laboratory of Bogor Institute of Agriculture, 3) Sidoardjo Village, Tepus Gunung Kidul. Variables being studied were nutritive value and protein digestibility of unenriched and enriched patilo with mujair FPH in several consumer acceptability in terms of flavour, taste, colour and texture. The study design was a complete random design of one factor in which the FPH was added in 5%, 10% and 15% concentration, each treatment repeated 3 times. Statistical analyses used for nutritive value and protein digestibility were Anova followed by Least Significant Difference test with 0,05 level of significancy Friedman and Wilcoxon Sign Rank test were used to test the organoleptic values both in the laboratory and in the field. Result:1) The nutritive value changes of raw enriched patilo were : a) significant decrease in water and carbohydrate content, b) significant increase in ash, fat and protein content compared to the unenriched patilo, 2) The nutritive value change of fried enriched patilo were : a) significant decrease in water and carbohydrate content, b) significant increase in fat, protein and energy content compared to the unenriched patilo, 3) The protein digestibility in raw and fried enriched patilo was significant higher than that without enrichement, 4) Patilo enriched with 10% mujair FPH was mostly liked by consumer either in the laboratory or in the field in term of taste, flavour and texture as for colour, all consumers prefered the unenriched patilo, 5) The changes of the nutritive values and protein digestibility were statistically significant in the enriched patilo, however from the nutrition point of view, only the increased of protein content (six folds increase) was meaningful. Conclusion: Patilo enriched with 10% mujair FPH is the most/well accepted by laboratory panelists and field consumers, and there was six folds increase in the protein content.   Key Word: Patilo, enrichment and fish protein hydrolysate.   ABSTRAK   Latar Belakang: Patilo adalah salah satu makanan jajanan tradisional Gunung Kidul yang diolah dari ampas singkong yang difermentasi dan dicampur dengan pati singkong. Kandungan protein dalam patilo sangat rendah, untuk itu perlu diperkaya antara lain dengan sumber protein hewani dari ikan air tawar baik dalam bentuk tepung ikan atau hidrolisat protein ikan (HPI). Tujuan: Penelitian ini bertujuan untuk 1). menganalisis nilai gizi patilo 2). menganalisis daya cerna protein patilo 3). menganalisis daya terima patilo goreng tanpa dan dengan penambahan HPI mujair Metode: Penelitian ini terdiri dari empat kegiatan yaitu: 1). pembuatan HPI mujair, 2). pembuatan dan pengkayaan patilo dengan HPI mujair, 3). pengujian nilai gizi dan daya cerna protein patilo tanpa dan dengan penambahan HPI mujair dan 4). uji penerimaan patilo baik di laboratorium maupun di lapangan. Variabel yang diamati adalah kandungan gizi patilo dan daya cerna protein patilo. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 1 faktor yaitu HPI0=tanpa penambahan HPI, HPI1= penambahan HPI 5% dari berat ampas (b/b), HPI2=penambahan HPI 10 % dari berat ampas (b/b) dan HPI3=penambahan HPI 15% dari berat ampas (b/b), masing-masing perlakuan diulang sebanyak tiga kali. Analisis kandungan gizi dan daya cerna patilo dengan Anova, uji dilanjutkan dengan uji Least Significant Difference (LSD) pada taraf dan 5%. Untuk analisis uji organoleptik baik di laboratorium maupun di lapangan dengan uji Friedman. Perbedaan hasil akan dilanjutkan dengan uji lanjut Wilcoxon Sign Rank Test. Hasil: 1) Nilai gizi patilo mentah dengan penambahan HPI mujair dibandingkan tanpa penambahan HPI mujair adalah : a) terjadi penurunan kadar air dan kadar karbohidrat secara signifikan (?<0,05) dan b) terjadi peningkatan kadar abu, kadar lemak dan kadar protein secara signifikan (?<0,05), 2) Nilai gizi patilo goreng dengan penambahan HPI mujair dibandingkan tanpa penambahan HPI mujair adalah : a). terjadi penurunan kadar air dan kadar karbohidrat secara signifikan (?<0,05) dan b).terjadi peningkatan kadar lemak, kadar protein dan energi secara signifikan (?<0,05) 3). Daya cerna protein patilo mentah dan goreng dengan penambahan HPI mujair meningkat secara signifikan (?=0,000) dibandingkan tanpa penambahan HPI mujair pada semua konsentrasi, 4). Patilo dengan penambahan HPI mujair 10% paling disukai oleh panelis agak terlatih dan panelis konsumen anak sekolah berdasarkan skor rasa, bau dan tekstur. Untuk warna, panelis agak terlatih dan panelis konsumen anak sekolah memilih patilo tanpa penambahan HPI mujair dan 5). Secara statistik perubahan nilai gizi dan daya cerna protein bermakna kecuali untuk kadar abu patilo goreng, namun dari sudut gizi perubahan yang berarti hanya pada kadar protein yang mencapai 6 kali lipat. Simpulan: Patilo yang diperkaya dengan HPI mujair 10% paling disukai oleh panelis agak terlatih maupun panelis konsumen anak sekolah dan terjadi kenaikan secara signifikan pada kadar protein yang mencapai 6 kali lipat. Kata Kunci : Patilo, pengkayaan dan hidrolisat protein ikan    Permalink : http://ejournal.undip.ac.id/index.php/jgi/article/view/3243  
Peningkatan Aktivitas Antioksidan Superoksida Dismutase pada Tikus Hiperglikemi dengan Asupan Tempe Koro Benguk (Mucuna pruriens L.) Retnaningsih, Christiana; Darmono, Darmono; Widiarnoko, Budi; Muis, Siti Fatimah
Agritech Vol 33, No 2 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1742.15 KB) | DOI: 10.22146/agritech.9803

Abstract

Hyperglycemia is associated with oxidative stress and the pathogenesis of diabetic complications. To reduce the hyperglycemia, modern treatment combined with traditional therapies through functional foods need to be considered, particularly to repair the damaged pancreas beta cells. A foodstuff that has a functionality potential is velvet beans (Mucuna pruriens L), espcially when it is in the form of a fermented product, i.e. tempe. The aim of study was to prove the effect of velvet beans tempe intake on blood glucose levels and superoxide dismutase antioxidant status. This research used randomized controlled group pre test- post test design using 50 male Sprague Dawley (SD) rats aged 2-3 months. The rats were subdivided into 5 groups, 10 rats per group, by means of random allocation. Group 1 is negative control (C-), group 2 is positive control (C+), group 3 is X1-TK10%, group 4 is X2-TK20%, group 5 is X3-TK30%. The groups of C+,X1, X2, X3 are induced by 40 mg/kg body weight stereptozotocin dose by intra peritoneal injection. The research was conducted for 30 days. The data were analyzed with Paired T Test, One-way Anova and continued by the Duncan’s Multiple Range Test. The results showed that streptozotocin injection increased the level of blood glucose and reduced the level of serum superoxide dismutase antioxidant activity. Bioassay experiment demonstrated that velvet bean tempe diet reduced the level of blood glucose. On the other hand velvet bean tempe diet improved the level of superoxide dismutase antioxidant activity.ABSTRAKHiperglikemi menimbulkan stress oksidatif dan patogenesis komplikasi diabetes. Untuk menurunkan hiperglikemi perlu dipertimbangkan kombinasi antara pengobatan modern dengan terapi tardisional melalui pangan fungsional guna mengurangi kerusakan sel beta pankreas. Bahan pangan yang memiliki potensi fungsional tersebut adalah biji koro benguk (Mucuna pruriens L) yang difermentasi menjadi bentuk tempe. Tujuan dari penelitian ini adalah untuk membuktikan pengaruh asupan tempe koro benguk terhadap kadar glukosa darah dan status antioksidan serum pada tikus hiperglikemi. Penelitian ini menggunakan 50 ekor tikus jantan jenis Sprague Dawley umur 2-3 bulan. Tikus dibagi ke dalam 5 kelompok dengan cara random alokasi. Kelompok 1 kontrol negatif (C-), kelompok 2 kontrol positif (C+), kelompok 3 adalah X1-TK10%, kelompok 4 adalah X2-TK10%, kelompok 5 adalah X3-TK10%. Tikus kelompok C+, X1, X2, X3 diinduksi streptozotocin (STZ) dengan dosis 40 mg/kg BB secara inta peritoneal. Penelitian dilakukan selama 30 hari. Data dianalisis dengan Paired T test, One way Anova dan dilanjutkan dengan Uji Wilayah Ganda Duncan. Hasil penelitian menunjukkan bahwa STZ meningkatkan kadar glukosa darah dan menurunkan aktivitas antioksidan SOD serum. Hasil analisis in vivo pada tkus menunjukkan bahwa asupan tempe koro benguk menurunkan kadar glukosa darah dan meningkatkan aktivitas antioksidan SOD serum.