Guntari Titik Mulyani
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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STUDI SISTEM RESPIRASI DAN KAJIAN MIKROBIOLOGIS LUMBA-LUMBA HIDUNG BOTOL INDO PASIFIK (TURSIOPS ADUNCUS) DARI PERAIRAN LAUT JAWA Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.858 KB) | DOI: 10.29244/avi.2.1.7-11

Abstract

Lumba-lumba hidung botol (bottlenose dolphin) adalah spesies lumba-lumba yang paling umum danpaling dikenal orang. Gangguan sistem respirasi pada lumba-lumba sering dijumpai, sementara jenisbakteri yang sering menyerang lumba-lumba dari perairan Laut Jawa belum pernah diteliti. Penelitianini bertujuan untuk mempelajari pemeriksaan klinis sistem respirasi lumba-lumba dan melakukan isolasibakteri serta jamur pada sistem respirasi bottlenose dolphin (Tursiops aduncus) dari Perairan Laut Jawa.Penelitian dilakukan dengan studi pustaka, pemeriksaan klinis serta isolasi sampel sistem respirasi. Swabdeep blowhole dilakukan terhadap sepuluh ekor bottlenose dolphin di PT. Wersut Seguni Indonesia.Sampel dikirim ke laboratorium Mikrobiologi FKH UGM untuk isolasi dan identifikasi terhadap bakteridan jamur. Hasil penelitian menunjukan bahwa pemeriksaan klinis lumba-lumba hanya dapat dilakukansecara inspeksi. Hasil isolasi ditemukan bahwa 5 dari 10 lumba-lumba (50%) positif Staphylococcus aureus,sedangkan jamur tidak ditemukan. Berdasarkan hasil penelitian, disimpulkan bahwa pemeriksaan klinislumba-lumba dapat dilakukan dengan cara inspeksi terhadap blowhole, tingkah laku hewan, adanya?chuff? atau ?honk?, frekuensi respirasi, sosialisasi hewan dan posisi hewan saat berenang. BakteriStaphylococcus aureus dijumpai pada sistem pernafasan lumba-lumba hidung botol dari perairan LautJawa di PT. Wersut Seguni Indonesia.
PEMBELIAN TERNAK DAN KELEMBABAN TINGGI MERUPAKAN FAKTOR RISIKO LEPTOSPIROSIS PADA SAPI DI GIRIMULYO, KULON PROGO, JOGJAKARTA (PURCHASE OF CATTLE AND HIGH HUMIDITY WERE RISK FACTORS OF BOVINE LEPTOSPIROSIS IN GIRIMULYO, KULON PROGO DISTRICT) Mulyani, Guntari Titik; Sumiarto, Bambang; ., Yuriati
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Leptospirosis is an acute infectious disease could to attack humans and animals such as dogs, cats,cows, pigs, and wild animals such as rats, weasels and squirrels. The death rate of human leptospirosis inIndonesia reached 2.5 to 16.5%. Leptospirosis attacked approximately 274 residents, 18 fatalities atKulonprogo disctrict, and designated as Extraordinary Events in 2011. The purpose of this reasearch is todetermine risk factors affecting the prevalence of leptospirosis in cattle in the area of human leptospirosisin Girimulyo, Kulon Progo. This study used 132 samples of cattle blood. Methods of examination withMicro Aglutination Test (MAT) conducted at the Center for Veterinary Research, Bogor. Micro AglutinationTest is done by creating a serum dilution with PBS in stages then added antigen in the form of various cultures leptospira serovar. Subsequently incubated at 28-30 ° C for 2 hours, and was reading the resultsunder a microscope dark field / phase contrast. Endpoint reading was 50% agglutination, highest finalserum dilution in serum-antigen mixtures that showed 50% agglutination is called the titer. Serum witha titer of 1:100 or more against one or more tested positive for serovar. Micro Aglutination Test result wasthe dependent variable (Y), while the independent variables (X) were factors of farmers, animal factors,enclosure factors, and feed factors. Univariate analysis was done to see descriptively each variable studied.Association between the prevalence of leptospirosis and risk factors was analyzed by Chi Square.Multivariate Logistic Regression Analysis was used to build models of leptospirosis disease to investigatethe risk factors associated with leptospirosis. Variables that influence the prevalence of leptospirosiswere measured magnitude of association by Odd Ratio. The results of study that the prevalence of bovineleptospirosis in Girimulyo subdistrict, Kulonprogo district was 7.6%. Risk factors for the prevalence ofleptospirosis in cattle in Girimulyo subdistrict, Kulonprogo district were livestock purchased factor (OR5.49) and a high humidity factor (OR 1.65). These results can be concluded that leptospirosis infection incattle is high (7.6%) in the area of human leptospirosis. The possibility cattle as a source of infection inhumans still needs further research.
THE EFFECT OF HIGH FAT DIET ON THE CONCENTRATION RATIO BETWEEN PROOXIDANT AND TOTAL ANTIOXIDANT IN RAT (SPRAGUE DAWLEY) Mulyani, Guntari Titik; Wuryastuti, Hastari
Jurnal Sain Veteriner Vol 17, No 1 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8589

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The objective of the investigation was to study the relationship between ratio prooxidant and total antioxidant (peroxidation index) and cell damage in male rats. Fourty male Sprague dawley rats, 3 month of age, 150-200 g of body weight were alloted randomly to 2 groups of 20 each. The first group was fed normal diet (control), the second group was fed high fat diet (20% fat). After 2 weeks, the blood samples were taken out from 5 rats of each group for measur­ing prooxidants and antioxidants. The prooxidants and anti-oxidants st-atus in this study was done by measuring thio barbituric add subs tance I TEARS) and total antloxldant status (TAS) concentration. The heart, liver and kidney tissues were collected for histopatological analysis, Perox­idation index (PI) were determined by dividing TBARS value with TAS value. The same procedures were repeated after 4, 8, and 16 weeks. Analysis of the data showed that after 16 weeks on experimental diet, the mean concentration of TBARS in blood of group I was 1.45Q+G.158 umol/L and the mean concentration of TBASS in blood of group 11 was 4,75 + 2,17 nmol/L. The highest ratio between prooxidant and total antioxidant was 17.47 and the Lowest ratio between oxidant and total antioxidant was 0.849. Analysis of histopatology showed that there were hyalinitation and oardiomyopathy in the heart tissu, fatty liver degeneration and necrosis in the liver and necrose and atrophy of the kidney. From the results can be concluded that (1) feeding high fat diet induced high ratio between proojiidant and antioxidant con^ centration (peroxidation Index). (2) Cell damage can be caused by high peroxidation index. (3) Individual variation might be caused by genetic factor.
IDENTIFIKASI SEROVAR PENYEBAB LEPTOSPIROSIS PADA ANJING DI YOGYAKARTA Mulyani, Guntari Titik; Hartati, Sri; Wuryastuty, Hastari; Tjahajati, Ida; Yuriadi, Yuriadi; Widiyono, Irkham; Yanuartono, Yanuartono; Purnamaningsih, Hary; Indarjulianto, Soedarmanto; Raharjo, Slamet; Nururozi, Alfariza; Ganapragasam, Angeline; Jiao, Yeo Suan
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.014 KB) | DOI: 10.22146/jsv.39201

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Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
KEJADIAN LEPTOSPIROSIS PADA ANJING DI DAERAH ISTIMEWA YOGYAKARTA (CASE OF CANINE LEPTOSPIROSIS IN THE CITY OF YOGYAKARTA) Mulyani, Guntari Titik; Hartati, Sri; Santoso, Yuli; Kurnia, Kurnia; Pramono, Agung Budi; Wirapratiwi, Dyah Kunti
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.3.403

Abstract

Leptospirosis is a zoonotic disease, which is caused by Leptospira interrogans. The incidence of leptospirosis in dogs varies according to region and season, and is considered as emerging infectious diseases in humans. Clinical symptoms of leptospirosis in dogs vary greatly, some dogs are asymptomatic, with mild symptoms, and others progress to severe illness until death. The study aims to determine cases of canine leptospirosis in Yogyakarta, and identify the serovar that infect them. A total of 20 dogs without symptoms, with mild symptoms, to severe symptoms of leptospirosis were collected their serum and further tested using Microscopic Agglutination Test (MAT) conducted at the Center for Veterinary Research (BBLitvet) Bogor. History of vaccination is recorded as a consideration in interpreting the MAT results. The results showed that three out of the 20 samples were positive leptospirosis. Of the three positive samples, one was identified as serovar bataviae, another one as serovar bataviae and tarrasovi, and the last as serovar bataviae, tarrasovi, ichterohaemorrhagiae, canicola, celledoni, pyrogenes, cynopteri, and rachmati, respectively. The three dogs with leptospirosis showed similar clinical symptoms i.e. Anorexia, lethargy, and fever. It can be concluded that there are cases of canine leptospirosis in Yogyakarta which is predominantly caused by Leptospira interrogans serovar bataviae. ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh Leptospira interrogans. Kejadian leptospirosis pada anjing bervariasi menurut wilayah dan musim, dan dianggap sebagai penyakit menular yang muncul pada manusia. Gejala klinis leptospirosis pada anjing sangat bervariasi, beberapa anjing tanpa menunjukkan gejala, dengan gejala ringan, dan yang lain melanjut menjadi penyakit yang parah sampai kematian. Penelitian ini bertujuan untuk mengetahui kasus leptospirosis pada anjing di Propinsi Daerah Istimewa Yogyakarta, dan mengidentifikasi serovar yang menginfeksinya. Sebanyak 20 ekor anjing tanpa gejala, dengan gejala ringan, sampai gejala parah leptospirosis diambil serumnya guna pemeriksaan Microscopic Agglutination Test (MAT) yang dilaksanakan di Balai Besar Penelitian Veteriner (BBLitvet), Bogor. Sejarah vaksinasi dicatat sebagai bahan pertimbangan dalam menginterpretasi hasil pemeriksaan MAT. Hasil penelitian menunjukkan bahwa tiga dari 20 sampel positif leptospirosis. Satu sampel positif terhadap serovar Bataviae, satu sampel positif terhadap serovar Bataviae dan Tarrasovi, dan satu sampel lagi positif terhadap serovar Bataviae, Tarrasovi, Ichterohaemorrhagiae, Canicola, Celledoni, Pyrogenes, Cynopteri, dan Rachmati. Anjing yang positif leptospirosis menunjukkan gejala klinis yang sama berupa anoreksia, kelemahan, dan demam. Dari hasil penelitian ini dapat disimpulkan bahwa terdapat kasus leptospirosis pada anjing di Yogyakarta yang penyebabnya dominan oleh Leptospira interrogans serovar Bataviae.
PENGARUH PENANGKARAN TERHADAP PROFIL ERITROSIT LUMBA-LUMBA HIDUNG BOTOL DARI PERAIRAN LAUT JAWA Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3636.309 KB) | DOI: 10.22146/jsv.2467

Abstract

Di laut Jawa banyak populasi lumba-lumba yang sering tersangkut jala para nelayan saat mencari ikan dilaut. Di Indonesia, lumba lumba hidung botol merupakan mamalia air yang dilindungi. Penelitian ini bertujuanuntuk mengetahui profil eritrosit bottlenose dolphin (lumba-lumba hidung botol) sebelum dan setelah penangkaran. Tujuh ekor lumba-lumba digunakan dalam penelitian ini. Sampel darah diambil melalui vena superfisialis sirip ekor guna pemeriksaan eritrosit, meliputi jumlah, morfologi, kadar hemoglobin dan hematokrit. Pengambilan sampel dilakukan saat lumba-lumba diangkat dari perairan Laut Jawa dan setelah lumba-lumba mengalami penangkaran di PT. WSI, Kendal, Jawa Tengah. Data sebelum dan sesudah penangkaran dibandingkan dan diuji dengan analisis statistik t-test berpasangan pada tingkat signifikasi 95%. Hasil penelitian menunjukkan adanya peningkatan jumlah eritrosit, kadar hemoglobin dan nilai hematokrit secara signifikan. Dari hasil penelitian ini dapat disimpulkan bahwa penangkaran yang dilakukan oleh PT. WSI,Kendal, Jawa Tengah meningkatkan profil eritrosit lumba-lumba hidung botol.Kata kunci : lumba-lumba hidung botol, captivity, profil eritrosit, vena superficial ekor, uji t-berpasangan
PREVALENSI DAN SEROVAR PENYEBAB LEPTOSPIROSIS PADA DOMBA DI KABUPATEN KULON PROGO Mulyani, Guntari Titik; Sulistyadi, Eko; Kirwanto, Antoni; Haryadi, Haryadi; Widuri, Ambar; Atmojo, Tri; Pramundari, Anis
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.253 KB) | DOI: 10.22146/jsv.22817

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Leptospirosis is a zoonotic disease, caused by Leptospira interrogans. A source of transmission in human leptospirosis are rodents, livestock, pets and wild animals. The prevalence of leptospirosis in cattle soon after theoutbreak of leptospirosis in humans that occurs in Kulon Progo district in 2011, reached 3.4% by the various serovar Leptospira. Breeding conditions of the people who still puts cattle and sheep in a single environment which enables transmission of leptospirosis in cattle. The purpose of this study was to determine the prevalence of leptospirosis and identify serovar caused of leptospirosis in sheep in Kulon Progo. A total of 60 sheep were done blood collection from the jugular vein 5 ml, serum was separated for leptospirosis examination with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospira serovar, namely: chterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Auatralis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. Leptospirosis prevalence rate was calculated by dividing the result by the number of MAT positive samples examined. Serovar types that give a positive agglutination result was serovar that caused leptospirosis in sheep. The results showed that two samples were positive against antigen serovarIchterohaemorrhagiae. Based on these results can be concluded that the prevalence of leptospirosis in sheep in Kulon Progo district were 3.3%. The cause of leptospirosis in sheep in Kulon Progo was Leptospira interrogansIchterohaemorrhagiae serovar.
LEPTOSPIROSIS PADA KUCING DI YOGYAKARTA DAN SEKITARNYA (FELINE LEPTOSPIROSIS IN YOGYAKARTA AND ITS SURRONDING) Mulyani, Guntari Titik; Raharjo, Slamet; Purnomo, Agung Budi; Santoso, Yuli; Kurnia, Kurnia; Wirapratiwi, Dyah Kunti
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.4.446

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Leptospirosis is a zoonotic disease that infects almost of mammals. The infecting microorganism is the Leptospira interrogans, which have many serovars. Clinical leptospirosis is rare in cats, but cats may shed leptospire in the urine to other animals, humans or environment. The aims of this research is to study feline leptospirosis in Yogyakarta and its surrounding. A total of 27 cats consisting of 3 groups of cats: household cats, stray cats, and cats with leptospirosis symptom were used in this research. The blood from all cats were taken 3 ml from cephalica vein, the serum were separated for Microscopic Agglutination Test (MAT). The examination were   conducted in Bbalitvet Bogor for 14 Leptospira serovar: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, dan Tarrasovi. The results showed that 1 stray cat was positive for Tarrasovi serovar. From the results of this study can be concluded that there was subclinic feline leptospirosis in Yogyakarta that caused by Leptospira interrogans Tarrasovi serovar.
ISOLASI DAN IDENTIFIKASI STAPHYLOCOCCUS AUREUS PADA SUSU ANJING DI WILAYAH YOGYAKARTA Mulyani, Guntari Titik; Hapsari, Andriani Dwi; Indarjulianto, Soedarmanto; Untari, Tri; ., Yanuartono; Raharjo, Slamet; Purnamaningsih, Hary
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.414

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EFEK RANSUM KOLESTERL TINGGI TERHADAP RASIO OKSIDAN DAN ANTIOKSIDAN PADA TIKUS SPRAGUE DAWLEY Mulyani, Guntari Titik; Wuryastuti, Hastari
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1464.135 KB) | DOI: 10.22146/jsv.477

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