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Repellency Test of Wet Tissue Containing DEET (N,N-diethyl- 3-metatoluamide) and Citronella Oil (Cymbopogon citratus) Againts Aedes Aegypti Mosquitoes Satoto, Tri Baskoro T.; Mulyaningsih, Budi; Sintorini, M.M.; Sugiarto, A.F.; Kesuma, B.A
Jurnal Kesehatan Masyarakat Nasional Vol. 5 No. 4 Februari 2011
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.486 KB) | DOI: 10.21109/kesmas.v5i4.135

Abstract

Dengue hemorrhagic fever has been one of the major health problems in Indonesia. As the disease spreads out by Aedes aegypti, a variety of ways has been conducted to disconnect host, agent, and the environment chain including prevention of human contact with the vector using by various repellents.The present test compared the complete protection time of N,N-diethyl- 3-metatoluamide (DEET) and citronella oil impregnated in wet tissue against Aedes aegypti. For this test, an ethanol-based 12.5% DEET and 20% citronella oil were prepared, into which dry tissue papers were immersed, drain in open air, and then stored in aluminum foil packs for one day and one week. Meanwhile, a number of disease-free adult female Aedes aegypti were placed in mosquito cages. The prepared one-day and oneweek stored wet tissues were used to swab volunteer adult human hands (from elbow to wrist) which were then inserted into the mosquito cage in three replicates (25 mosquitoes each cage). Elapsed time from first hand insertion to the first mosquito bite was calculated and expressed as repellency time of the impregnated repellent. It was found that the average repellency times of one-day stored 12.5% DEET and 20% citronella oil wet tissues were 4 hour 26 minutes and 14.24 minutes, respectively, while for oneweek stored were 4 hour 6 minutes and 12.57 minutes, respectively. Post Hoc test showed that the repellency time difference between one-day and one-week storage was not statistically significance (p = 0.524 for DEET and p = 0.681 for citronella oil). Key words: Aedes aegypti, DEET, citronella oil, wet tissueAbstrakDemam berdarah merupakan salah satu masalah utama kesehatan di Indonesia. Karena penyakit ini disebarkan oleh nyamuk Aedes aegypti, berbagai cara telah dilakukan untuk memutus rantai pejamu, agen, dan lingkungan, termasuk mencegah kontak manusia dengan vektor dengan menggunakan bermacam-macam pengusir serangga. Uji ini membandingkan waktu proteksi N,N-diethyl-3-metatoluamide (DEET) dengan wak tu proteksi minyak sitronella yang terkandungkan dalam tisu basah terhadap Aedes aegypti. Untuk uji ini, dibuat larutan 12,5% DEET dalam etanol dan larutan 20% minyak sitronella. Sejumlah tisu kering dicelupkan ke dalam masing-masing larutan ini, ditiriskan dalam udara terbuka, kamudian dibungkus dengan lembaran aluminium dan disimpan selama satu hari dan satu minggu. Sementara itu, sejumlah nyamuk Aedes aegypti betina yang bebas penyakit ditempatkan dalam sangkar. Tisu basah yang telah disimpan selama satu hari dan satu minggu diusapkan pada bagian tangan (dari sikut sampai pergelangan tangan) relawan, kemudian tangan yang telah diusap itu dimasukkan ke dalam sangkar nyamuk dalam tiga replikasi uji (25 ekor nyamuk per sangkar). Waktu antara pemasukan tangan sampai dengan gigitan nyamuk pertama dinyatakan sebagai waktu usir tisu basah. Ditemukan bahwa waktu usir rata-rata tisu basah 12,5% DEET dan 20% minyak sitronella yang disimpan satu hari masing-masing 4 jam 26 menit dan 14,24 menit, sedangkan untuk tisu basah yang disimpan satu minggu masing-masing 4 jam 6 menit dan 12, 57 menit. Uji Post Hoc menunujukkan bahwa penyimpanan tisu basah satu hari dan satu minggu tidak berbeda secara bermakna (p = 0,524 untuk DEET dan p = 0,681 untuk minyak sitronella).Kata kunci: Aedes aegypti, DEET, minyak sitronella, tisu basah
PHYLOGENETIC ANALYSIS OF CULEX TRITAENIORHYNCHUS AND CULEX VISHNUI VECTOR OF JAPANESE ENCEPHALITIS VIRUS Astuti, Raden Roro Upiek Ngesti Wibawaning; Mulyaningsih, Budi; Soesilohadi, R.C. Hidayat; Nurcahyo, Raden Wisnu; Hadisusanto, Suwarno
Global Medical & Health Communication (GMHC) Vol 7, No 3 (2019)
Publisher : Faculty of Medicine Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v7i3.4051

Abstract

Culex tritaeniorhynchus and Culex vishnui are medically important mosquitoes that transmit Japanese Encephalitis (JE) virus. There is less information about the recording data and research due to genetic character differences among them. The objective of this study was to examine the genetic variation of Cx. tritaeniorhynchus and Cx. vishnui in 3 site of Central Java using PCR-RAPD.  The study was done from January to November 2017, in Pekalongan City, Pekalongan Regency and Semarang Regency. Adult female mosquitoes were collected by landing bite method. DNA of ten Cx. tritaeniorhynchus  samples and fifteen samples of Cx. vishnui were purified using  DNA kit extraction. Furthermore, PCR amplification was conducted with 5 RAPD primers (OPA 11, 12, 15, 16, and 20) and would run into 2% gel electrophoresis for 45 minutes. Cluster analysis was done by using MVSPTM software. The results showed  213 genetical characters of Cx. vishnui, while 142 characters were shown by Cx. tritaeniorhynchus. The dendograms showed 3 distinct grups of Cx. vishnui from 2 sites of Pekalongan and 1 site of Semarang, while Cx. tritaeniorhynchus showed 2 distinct grups, which were 1 group from Pekalongan and 1 group  from Semarang. Low genetic similarity (<10%) was shown Cx. vishnui from Pekalongan City and Pekalongan District, and there was no genetic similarity in Cx. tritaeniorhynchus from Pekalongan and from Semarang. It is concluded that the polymorphism of Cx tritaeniorhynchus and Cx. vishnui reached 100%.
Larvicidal Activity of The Mixture of Cashew Nut Shell Liquid (CNSL) and Aqueous Extract of Sapindus rarak DC Against Larvae of Culex quinquefasciatus Fauziah, Rahmi Safarina; Sudarsono, Sudarsono; Mulyaningsih, Budi
Biology, Medicine, & Natural Product Chemistry Vol 3, No 1 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.723 KB) | DOI: 10.14421/biomedich.2014.31.21-23

Abstract

The aim of this study was to evaluate the larvicidal activity of Cashew Nut Shell Liquid (CNSL) against the Culex quinque fasciatus in larval stage. The CNSL was diluted in water by addition of aqueous extract of Sapindus rarak DC to increase its solubility. Larvae were exposed to varying concentrations of that mixture. The larvae mortality was observed after 24 h exposure. LC50 and LC90 value by extrapolation were 20,52 ppm and 55,41 ppm respectively. CNSL were specified by characterizing its physico-chemical properties and  anacardic acid as marker compound by High Performance Chromatography (HPLC). The results were the mixture of Cashew Nut Shell Liquid (CNSL) and Aquous extract of Sapindus rarak DC had larvicidal activity against Cx. Quinque-fasciatus and further investigations were needed to identify the fatty acid derivative as active compound of CNSL which  responsible for larvicidal activity.
INSENCE COMBUSTIBLE SEREH, CENGKEH DAN JERUK SEBAGAI PENOLAK NYAMUK AEDES AEGYPTI Mulyani, Sri; Mulyaningsih, Budi; Lestari, Anindita Winda; Munawaroh, Fitri Ana; Anna, Diescendy Selly
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.532 KB) | DOI: 10.14499/mot-TradMedJ18iss3pp195-200

Abstract

Nyamuk merupakan salah satu serangga vektor penyakit seperti demam berdarah, malaria, filariasis, dan sebagainya. Penggunaan bahan yang memiliki aktivitas repelan (penolak) seperti sereh, daun jeruk, daun cengkeh merupakan salah satu cara untuk melindungi diri dari gigitan nyamuk. Sediaan dupa (insence combustible) dikenal sebagai sediaan aromaterapi yang dapat digunakan setiap saat, yang umumnya digunakan pada acara-acara keagamaan. Pada penelitian ini dimaksudkan untuk membuat sediaan insence combustible (dupa) yang dapat berfungsi ganda, yaitu sebagai penyegar ruangan juga dapat berfungsi sebagai repelan terhadap nyamuk Aedes aegypti. Penelitian dilakukan dengan membuat sediaan insence combustible dengan  bahan aktif herba sereh, daun cengkeh, dan jeruk, dengan 5 variasi kadar 0,10, 20, 40, 80%. Kemudian sediaan diujikan pada 25 ekor nyamuk, yang terdapat di dalam sangkar nyamuk dobel trap. Jumlah nyamuk di kotak A dan B  dihitung pada waktu 5, 10, 15, 30, 45, dan 60 menit. Selanjutnya dihitung persentase aktivitas repelannya. Juga dilakukan pengujian profil kromatogram bahan uji dengan metoda mikrodestilasi tanur TAS dengan beberapa pereaksi identifikasi. Hasil menunjukkan, bahwa sediaan yang dibuat semua memiliki aktivitas penolak nyamuk dengan kadar efektif  untuk sereh adalah 40%, jeruk 20%, dan cengkeh 10%.  Profil kromatogram menunjukkan daun jeruk memperlihatkan bercak paling banyak untuk senyawa terpenoid dan fenolik, diikuti cengkeh dan sereh. Bercak sebagai senyawa dengan gugus karbonil (aldehid dan keton) hanya ditunjukkan oleh herba sereh.
The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti Cahyati, Widya Hary; Asmara, Widya; Umniyati, S Rahmah; Mulyaningsih, Budi
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017): Jurnal KEMAS Vol.12 No.2 : January 2017
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6223

Abstract

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and &lt;1,20 ppm (µg/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ≤68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.
THE EFFECT OF ANTICOAGULANT IN BLOOD MEAL SOURCE ON THE AEDES AEGYPTI REPRODUCTIVE ABILITY IN LABORATORY Lusiyana, Novyan; Mulyaningsih, Budi; Umniyati, Sitti Rahmah
Tropical Medicine Journal Vol 3, No 2 (2013): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.018 KB) | DOI: 10.22146/tmj.5872

Abstract

ABSTRACTIntroduction: Aedes aegypti is one of the major vectors of dengue hemorrhagic fever (DHF) that can be reared in laboratory. Artificial membrane feeding (AMF) assay is used as a simulated host to blood feeding mosquitoes in laboratory.Objectives: The purpose of this study are to investigate the effect of heater and the most widely used anticoagulant of K3EDTA, heparin and sodium citrate on blood feeding sucsses, feeding rate, fecundity, hatchability, preadult development and survival rate of Aedes Aegypti colonies maintained by AMF system compared to direct human feeding.Methods: The system consisted of AMF with parafilm membrane which are warmed and not warmed by a waterbath. Human blood samples were used to feed Aedes aegypti using AMF. The number of eggs were counted seven days after feeding after mosquitoes laid the eggs. Every eggs were hatched in a 500 mL of rearing glass to evaluate the hatchability and preadult development. Survival rate is evaluated after blood feeding until 30 days. Data was analyzed using one-way ANOVA and paired t-tests and a p value <0.05 considered as significantly difference.Results: Blood feeding success of Aedes aegypti was not significantly differ when offered blood meal using anticoagulant heparin with heater (82.22%) compare to that of control groups (81.67%) (p=0.917). There was a significant difference in feeding rate (p=0.000), fecundity (p=0.000), hatchability (p=0.000) between groups. All results were higher in heparin than K3EDTA and sodium citrate, but in pre adult development and survival rate K3EDTA showed better result than that of control, heparin and sodium citrate groups. So this anticoagulant was acceptable for maintenance of laboratory colonies of Aedes aegypti.Conclusion: We conclude that heater can increase the blood feeding sucsses. The K3EDTA, heparin and sodium citrate can affect the feeding rate, fecundity, hatchability, and preadult development, but do not affect survival rate. Heparin can be used for routine colonization of laboratory strain of Aedes aegypti with  AMF assay.Keywords: Anticoagulants, artificial membrane feeding, reproductive ability, survival rate
The Phytochemical Analysis of Hay Infusions and Papaya Leaf Juice as an Attractant Containing Insecticide for Aedes Aegypti Cahyati, Widya Hary; Asmara, Widya; Umniyati, S Rahmah; Mulyaningsih, Budi
KEMAS: Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.6223

Abstract

Aedes aegypti mosquito population could be controlled by using lethal ovitrap. The addition of hay infusions as a attractant greatly enhance Aedes aegypti eggs trapped, and papaya leaf juice may inhibit Aedes aegypti eggs evolve to larvae or a larvae to pupae stage. This study was conducted to find out the chemical compounds in hay infusion and papaya leaf juice. We used phytochemical test using UV-Vis Spectrophotometry, Thin Layer Chromatography, and High Performance Liquid Chromatoraphy (HPLC) method. The results showed that hay infusion contains 12,75 mg/L ammonium and <1,20 ppm (g/mL) lactic acid and papaya leaf juice contains 0,25% alkaloid, 0,14% flavonoid, 0,30% saponin, ?68 mg/L steroid and 11,34% tannin, but negative terpenoid. We concluded that hay infusion and papaya leaf juice contains chemical compounds that could be use as attractant and bioinsecticide to Aedes aegypti, respectively.
LEMONGRASS, CLOVES, ORANGE LEAVES AS INSENCE COMBUSTIBLE FOR AEDES AEGYPTI REPELLANT Mulyani, Sri; Mulyaningsih, Budi; Lestari, Anindita Winda; Munawaroh, Fitri Ana; Anna, Diescendy Selly
Majalah Obat Tradisional Vol 18, No 3 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.532 KB) | DOI: 10.22146/tradmedj.8225

Abstract

Mosquitoes are one of the insect vector-borne diseases such as dengue, malaria, filariasis, etc. The use of materials whose got repellent activity such as lemongrass, cloves, and orange leaves are one of the best way to be protected from mosquito bites. Combustible insence known as aromatherapy preparations that can be used at any time, which is commonly used in religious events. This research is intended to make preparations of combustible insence which has dual functions, serve as room fresheners and also as repellent against Aedes aegypti. The research is conducted by making preparations of the active ingredient of lemongrass, cloves, and orange leaves, each with 5 different concentrations. Those preparats are tested on 25 mosquitoes contained in double trap mosquito cages. The number of mosquitoes in box A and B will be calculated at 5, 10, 15, 30, 45, and 60 minutes respectively. Thus, the repellent activity can be determined. Chromatogram profile testing is also being conducted using TAS microdestilation metode with several reagents for identification. Result shown that all preparations have mosquito repellent activity with 40% of effective concentration for lemongrass, 20% for orange leaves, 10% for cloves leaves. Chromatogram profile test shows orange leaves have the most spots for terpenoid and fenolic compound, followed by cloves and lemongrass. Spots as compounds with carbonyl groups (aldehydes and ketones) is only found in lemongrass.
Repellency Test of Wet Tissue Containing DEET (N,N-diethyl- 3-metatoluamide) and Citronella Oil (Cymbopogon citratus) Againts Aedes Aegypti Mosquitoes Satoto, Tri Baskoro T.; Mulyaningsih, Budi; Sintorini, M.M.; Sugiarto, A.F.; Kesuma, B.A
Jurnal Kesehatan Masyarakat Nasional Vol. 5 No. 4 Februari 2011
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.486 KB) | DOI: 10.21109/kesmas.v5i4.135

Abstract

Dengue hemorrhagic fever has been one of the major health problems in Indonesia. As the disease spreads out by Aedes aegypti, a variety of ways has been conducted to disconnect host, agent, and the environment chain including prevention of human contact with the vector using by various repellents.The present test compared the complete protection time of N,N-diethyl- 3-metatoluamide (DEET) and citronella oil impregnated in wet tissue against Aedes aegypti. For this test, an ethanol-based 12.5% DEET and 20% citronella oil were prepared, into which dry tissue papers were immersed, drain in open air, and then stored in aluminum foil packs for one day and one week. Meanwhile, a number of disease-free adult female Aedes aegypti were placed in mosquito cages. The prepared one-day and oneweek stored wet tissues were used to swab volunteer adult human hands (from elbow to wrist) which were then inserted into the mosquito cage in three replicates (25 mosquitoes each cage). Elapsed time from first hand insertion to the first mosquito bite was calculated and expressed as repellency time of the impregnated repellent. It was found that the average repellency times of one-day stored 12.5% DEET and 20% citronella oil wet tissues were 4 hour 26 minutes and 14.24 minutes, respectively, while for oneweek stored were 4 hour 6 minutes and 12.57 minutes, respectively. Post Hoc test showed that the repellency time difference between one-day and one-week storage was not statistically significance (p = 0.524 for DEET and p = 0.681 for citronella oil). Key words: Aedes aegypti, DEET, citronella oil, wet tissueAbstrakDemam berdarah merupakan salah satu masalah utama kesehatan di Indonesia. Karena penyakit ini disebarkan oleh nyamuk Aedes aegypti, berbagai cara telah dilakukan untuk memutus rantai pejamu, agen, dan lingkungan, termasuk mencegah kontak manusia dengan vektor dengan menggunakan bermacam-macam pengusir serangga. Uji ini membandingkan waktu proteksi N,N-diethyl-3-metatoluamide (DEET) dengan wak tu proteksi minyak sitronella yang terkandungkan dalam tisu basah terhadap Aedes aegypti. Untuk uji ini, dibuat larutan 12,5% DEET dalam etanol dan larutan 20% minyak sitronella. Sejumlah tisu kering dicelupkan ke dalam masing-masing larutan ini, ditiriskan dalam udara terbuka, kamudian dibungkus dengan lembaran aluminium dan disimpan selama satu hari dan satu minggu. Sementara itu, sejumlah nyamuk Aedes aegypti betina yang bebas penyakit ditempatkan dalam sangkar. Tisu basah yang telah disimpan selama satu hari dan satu minggu diusapkan pada bagian tangan (dari sikut sampai pergelangan tangan) relawan, kemudian tangan yang telah diusap itu dimasukkan ke dalam sangkar nyamuk dalam tiga replikasi uji (25 ekor nyamuk per sangkar). Waktu antara pemasukan tangan sampai dengan gigitan nyamuk pertama dinyatakan sebagai waktu usir tisu basah. Ditemukan bahwa waktu usir rata-rata tisu basah 12,5% DEET dan 20% minyak sitronella yang disimpan satu hari masing-masing 4 jam 26 menit dan 14,24 menit, sedangkan untuk tisu basah yang disimpan satu minggu masing-masing 4 jam 6 menit dan 12, 57 menit. Uji Post Hoc menunujukkan bahwa penyimpanan tisu basah satu hari dan satu minggu tidak berbeda secara bermakna (p = 0,524 untuk DEET dan p = 0,681 untuk minyak sitronella).Kata kunci: Aedes aegypti, DEET, minyak sitronella, tisu basah
REPELLENT ACTIVITY OF CATNIP EXTRACT (NEPETA CATARIA L,) AGAINST AEDES AEGYPTI MOSQUITO AS DENGUE VECTOR Doraysamy, Doraysamy; Mulyaningsih, Budi; Ernaningsih, Ernaningsih
Tropical Medicine Journal Vol 2, No 2 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1054.91 KB) | DOI: 10.22146/tmj.17122

Abstract

ABSTRACTIntroduction: Dengue infection has been a major concern for decades. Prevention from getting bitten by Aedes aegypti mosquito is considered better than cure as there is no specific antiviral treatment for dengue. Recently, catnip (Nepeta cataria L.) extract has been formulated and marketed as an alternative for protection against mosquitoes and act as a natural form of mosquito repellent.Objectives: To understand the repelling properties of both DEET and N. cataria L. extract and to compare the repelling properties between DEET and N. cataria L. against Ae. aegypti mosquito.Methods: The repelling properties was tested based on the number of mosquitoes that sits on the hand of the respondent after being applied to the gold standard of mosquito repellent, DEET, and compared to N. cataria L. extract in different concentrations in total of 15 minutes.Results: Three different concentrations of catnip extract (10%, 30% and 50%) shows different efficacy in repelling Ae. aegypti mosquitoes at the range of 62.8%-80%, although the repellent activity is still lower than DEET that have 100% repellent activity.Conclusion: Catnip (N. cataria L) showed mosquitoes repellent properties at concentration 10-50% with repellent activity at the range 62.8%-80%. Keywords: Dengue, Aedes aegypti, repellent, DEET (N,N- Diethyl-m-toluamide), catnip (Nepeta cataria L.) extract. INTISARI Pendahuluan: Infeksi Dengue merupakan masalah utama selama berpuluh tahun. Pencegahan dengan menghindari gigitan nyamuk Aedes aegypti dianggap lebih baik daripada mengobati karena tidak ada terapi antiviral yang spesifik untuk dengue. Akhir-akhir ini, ekstrak catnip (Nepeta cataria L.) telah diformulasi dan dipasarkan sebagai alternatif untuk perlindungan terhadap nyamuk dan berlaku sebagai bentuk alami dari repelen.Tujuan: Untuk memahami agen penolakan dari N,N- Diethyl-m-toluamide  (DEET) dan ekstrak N. cataria L. extract dan membandingkan agen penolakan antara DEET dan N. cataria L. terhadap nyamuk Ae. aegypti.Metode: Agen penolakan diuji berdasarkan jumlah nyamuk yang mendarat di tangan responden setelah diolesi repelen nyamuk baku DEET, dan dibandingkan dengan ekstrak N. cataria L. pada berbagai konsentrasi selama total waktu 15 menit.Hasil: Tiga konsentrasi ekstrak catnip (10%, 30% and 50%) menunjukkan daya penolakan nyamuk Ae. aegypti yang berbeda pada rentang 62.8%-80%, meskipun aktivitas penolakan nyamuknya masih lebih rendah dari DEET yang mempunyai aktivitas penolakan 100%.Simpulan: Ekstrak Catnip (N. cataria L) menunjukkan daya penolakan nyamuk pada konsentrasi 10-50% dengan aktivitas repelant antara 62.8%-80%.Kata kunci: Dengue, Aedes aegypti, repellent, DEET (N,N- Diethyl-m-toluamide), ekstrak catnip (Nepeta cataria L.).