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Bioassay-Guided Isolation of an Antibacterial Compound from the Indonesian Soft Coral Putra, Masteria Yunovilsa; Saparhadi, Ahmad; Karim, Firmansyah; Murniasih, Tutik; Swasono, Respati Tri
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.246

Abstract

With the aim of searching for new antibacterial compound from marine soft corals, the investigation had been conducted on antibacterial activity of an extract from soft corals Sarcophyton trocheliophorum in the n-hexane, ethyl acetate, n-butanol, and aqueous fractions. The antibacterial activity was tested against two Gram-positive bacteria, viz. Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923), and two Gram-negative bacteria, viz. Escherichia coli (ATCC 25922) and Vibrio cholerae (ATCC 14035) using the agar disc diffusion assay. Among them, the n-hexane fraction was the most active against three tested bacteria, viz. Bacillus subtilis, Staphylococcus aureus and Vibrio cholerae at the concentration 125 µg/ml, with inhibition zone 14.2, 18.2, 13.8 mm, respectively. Isolation and purification of the active component from the n-hexane fraction led to a known cembranoid-type diterpene, sarcophytoxide. The chemical structure of the isolated compound was determined by IR, MS and NMR, as well as compared to data from the literature. Sarcophytoxide showed moderate activity against B. subtilis, S. aureus and V. cholerae, with a minimum inhibitory concentration (MIC) of 125, 100, 125 mg/ml, respectively.
THE ANTIBACTERIAL COMPOUND COLLISMICIN A DERIVED FROM MARINE Streptomyces sp Q-629K Murniasih, Tutik; Adachi, Kyoko
Indonesian Journal of Chemistry Vol 8, No 3 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1675.632 KB) | DOI: 10.22146/ijc.21600

Abstract

In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis .   Keywords: antimicrobial, collismycin A and marine Streptomyces sp
Antioxidant Capacities of Holothuria Sea Cucumbers Murniasih, Tutik; Putra, Masteria Yunovilsa; Pangestuti, Ratih
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.217

Abstract

Sea cucumbers are a potential source of bioactive metabolites for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activityof several extracts of sea cucumbers, Holothuria scabra, Holothuria atra, Holothuria leocospilota, and Holothuria excellens collected from Lombok Island. Phytochemical screening revealed the presence flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH model were evaluated. The crude extract of H. leocospilota and H. atra showed the highest potential antioxidant capacity. H. atra extract was separated by column chromatography silica gel eluting with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. However, these are preliminary findings and further studies will have to focus on the isolation of bioactive substances derived from H atra and identify other health benefit effects of H. atra.
BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35, No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9518.239 KB) | DOI: 10.14203/mri.v35i1.4

Abstract

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.
CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11 Murniasih, Tutik; Bayu, Asep
Marine Research in Indonesia Vol 40, No 2 (2015)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.902 KB) | DOI: 10.14203/mri.v40i2.63

Abstract

Marine sponge Aaptos-aaptos is thought to produce antimicrobial aaptamine and its derivatives. To investigate whether its associated bacteria are in fact the producer of such bioactive compounds, a study of antibacterial compounds derived from Aaptos-associated bacteria was conducted. In this research, approximately 10 bacterial colonies were isolated from the sponge Aaptos aaptos. Among the bacteria isolated, the one that showed the most potential for producing antibacterial compounds was Rhodobacteracea bacterium. Extra and intracellular bacterial extract from this strain strongly inhibited the growth of pathogenic bacteria Staphylococcus aureus and Vibrio eltor, while were moderately effective against Bacillus subtilis. Optimization of antibacterial activity was conducted by culturing Rhodobacteraceae bacterium in various carbon sources such as glucose, lactose, amylum, molasses and glycerol. The highest production of biomass was obtained by culturing this bacteria in SYP (Seawater Yeast  Peptone) medium, enriched with 1% glycerol as the carbon source and with a harvesting time of around 56-104 hours. The highest activity (8 U/ml) was reached when culturing this strain in SYP medium without any adding of carbon sources. Data analysis using a statistical tool indicated that carbon sources added to medium do not have a significant effect on antibacterial activity. Characterizing the compound responsible for the antibacterial properties will be the topic of further work.Keywords : carbon source, Rhodobacteracea bacterium, Aaptos aaptos, associated bacteria 
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hani; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.451 KB) | DOI: 10.14203/ab.v20i1.234

Abstract

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.
The Investigation of Polycyclic Aromatic Hydrocarbon and Oil Degrading Bacteria Isolated from The Marina Port Ancol, Jakarta Bay Lisdiyanti, Puspita; Yopi, Yopi; Murniasih, Tutik
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/45

Abstract

Polycyclic Aromatic Hydrocarbons (PAHs) as well as crude oil are widespread environmental pollutants. The contamination of air, soil, freshwater (surface water and groundwater), and marine environments by PAHs as well as crude oil has been reported. Of concern to public health is the fact that many PAHs or their metabolites are mutagenic, carcinogenic, or both. North Java coastal area such as Jakarta Bay is the polluted marine area in Indonesia as a result from anthropogenic wastes and the oil spill. Although evaporation and photo-oxidation play an important role in oil detoxification, ultimate and complete degradation is accomplished mainly by marine micro flora, and being dominant in this function. Certain bacteria are well-known could consume and degrade the PAHs as well as crude oil. Therefore investigating the potential PAH and oil degrading marine bacteria is important. In this study, we collected sample from oil polluted area in Marina Port Ancol, Jakarta Bay and isolated four PAH substrates and Arabian crude oil degrading marine bacteria using enrichment method and direct isolation method. As result, 223 strains could degrade PAHs, among these strains, 94 strains could degrade phenanthrene, 23 strains degrade fluoranthene, 92 strains could degrade dibenzotiophen, 14 strains could degrade phenotiazin and 106 isolates degrade crude oil.Key words: polycyclic aromatic hydrocarbons, crude oil, degrading bacteria, bioremediation.
BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5544.715 KB) | DOI: 10.14203/mri.v33i2.484

Abstract

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batams coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.
Pengaruh Nutrisi Dan Suhu Terhadap Selektivitas Potensi Antibakteri Dari Bakteri Yang Berasosiasi Dengan Spons Murniasih, Tutik; Wibowo, Joko Tri; Putra, Masteria Yunovilsa; Untari, Febriana; Maryani, Mery
Jurnal Kelautan Tropis Vol 21, No 1 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.954 KB) | DOI: 10.14710/jkt.v21i1.2084

Abstract

 roduction of pharmacological activity by marine microorganism is strongly influenced by nutrition and environmental conditions. In this study would  discuss about the influence of several type of media to the production of antibacterial agent by sponge-. associated microorganisms. About 3 sponges tissue Theonella sp, Callispongia sp. and Lithistide sp. collected from Seribu Island will be used for the host of associated microorganism. Agar medium used for isolation were M1 that contained amylum,  yeast extract and peptone, M2 (10% marine broth media) contained yeast extract and peptone, M3 only sea water without adding any nutrients. Beside the nutrient variation,  heat sock treatment at 50oC toward the sponge solution also apply to this study. The bacterial isolation data indicated that bacterial density in (CFU/100µL) of  Theonella sp, Callispongia sp. and Lithistide sp. were minimum when spreading in M3 medium with heat sock treatment. This data showed that limiting in nutrient and heating could increasing bacterial selectivity. The antibacterial activity capability of bacterial strains isolated using M1, M2 and M3 respectively in range were 81,8-90,9%; 50-87,5%  and; 66,7 -100%. This results showed that less nutrient of media will rise the number of antibacterial activity strains,and decreasing of bacterial density. This study reported that the minimum nutrient of isolation media and heat shock treatment could be used for selecting the antibacterial strains of sponge associated bacteria.  Aktifitas farmakologi yang dihasilkan oleh mikroorganisme laut sangat dipengaruhi oleh nutrisi dan kondisi lingkungan. Hal tersebut mendorong untuk digali lebih dalam tentang aspek-aspek yang mempengaruhi seleksi mikroba potensial pada spons. Metode isolasi mikroba dari jaringan spons menjadi kunci dalam menguak potensi mikroba simbionnya. Dalam penelitian ini akan membahas pengaruh berbagai media isolasi bakteri yang berasosiasi dengan spons asal Kep. Seribu. Terhadap 3 specimen spons Theonella sp., Callispongia sp. dan Lithistide sp. dilakukan isolasi bakteri dengan metode direct sampling menggunakan media M1, M2 dan M3. Media M1 mengandung nutrisi antara lain amilum, ekstrak khamir dan pepton, sedangkan media M2 mengandung sumber nutrisi ekstrak khamir dan pepton dan M3 hanya media agar dan air laut. Selain variasi nutrient dalam media, perlakuan pemanasan pada suhu 50oC juga akan dilakukan terhadap larutan sampel spons sebelum dilakukan penyebaran pada media isolasi. Hasil isolasi bakteri yang diisolasi spons Theonella sp, Callispongia sp. dan Lithistide sp. menunjukkan bahwa kepadatan minimum diperoleh dengan menggunakan media M3 dengan perlakuan pemanansan. Dari data isolasi bakteri menunjukkan bahwa selain kandungan nutrient yang minimum, perlakuan pemanasan akan menurunkan kepadatan jumlah bakteri yang tumbuh, sehingga pemanasan merupakan salah satu cara dalam seleksi isolasi bakteri yang berasosiasi dengan spons. Hasil analisis aktivitas antibakteri menunjukkan bahwa persentase strain-strain bakteri yang aktif terhadap antimikroba Vibrio eltor, Eschericia coli dan Bacillus subtilis dengan variasi media M1 berkisar antara 81,8-90,9%;  M2 berkisar 50-87,5% dan M3 berkisar 66,7-100%. Dari data tersebut disimpulkan bahwa semakin sedikitnya nutrisi media isolasi maka semakin tingginya mikroba-mikroba potensial penghasil antibiotik. Media M3 merupakan media yang selektif untuk isolasi mikroba potensial dari spons, terbukti dengan tingginya prosentase bakteri yang aktif dan berkurangnya jumlah koloni yang tumbuh.
BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35 No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9518.239 KB) | DOI: 10.14203/mri.v35i1.4

Abstract

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.