Tridjoko Wisnu Murti
Department of Dairy Science and Milk Industry, Faculty of Animal Science, Gadjah Mada University

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Lactation Characteristic of Etawah Crossed Breed Goats Under Intensive Management Suranindyah, Yuni; Widyobroto, Budi Prasetyo; Astuti, Sulvia Dwi; Murti, Tridjoko Wisnu; Adiarto, Adiarto
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.393 KB) | DOI: 10.21059/buletinpeternak.v44i1.44176

Abstract

The study aimed to describe lactation characteristic of Etawah Crossed Breed goats raised under intensive management. The study located in BBPTU HPT Baturraden, Purwokerto, used 27 female pregnant goats, at 24 to 27 months old. The goats were managed followed standard operating procedure of the research institute. The data consisted of body size and body weight, colostrum and milk yield, lactation period and reproductive performance. The results showed the average of daily milk yield, lactation period, total milk and colostrum production in first lactation were 960±340 mL/day, 157±41 days, 114,720±68,900 mL and 415±240 mL/day. The proportion of monthly milk production from initial lactation to dry off were 21.27, 22.17, 20.18, 17.29, 12.52, 9.13, and 5.65% of total lactation yield, respectively. Peak production ranged from first to second months of lactation, the highest was in the week 4th, averaged of 1080 mL/day. Production persistency was 66.39%. The rate of increase toward peak was 5.60%/week and rate of decline from peak was -3.92% of milk yield in the previous week. Trend equation of lactation curve was Y = 8412.7 –233.65 x. Means period of postpartum mating, service per conception and body condition score were 88.7±39.2 days, 1.2 and 3.45, respectively. There was no correlation between postpartum mating, body condition score and milk production. The study concluded that Etawah Crossed Breed goat under intensive management had short lactation period, early peak production, low persistency. The dry period was estimated 3 months and pregnancy occurred after peak production. 
Development of Fermented Mare’s Milk Using Mixed Probiotic Cultures Murti, Tridjoko Wisnu; Robiyati, Eni; Jundi, H. L.; Ramadhani, F.; Rustamadji, B.; Suranindyah, Y. Y.
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.35 KB) | DOI: 10.5398/medpet.2016.39.1.9

Abstract

Mare’s milk has good nutrient composition for human being in the form of natural milk or milk products. It can be used to replace cow’s milk, especially in the regions outside of Java Island which are rarely found dairy cow. This study had an objective to develop fermented mare’s milk by using mixed cultures of probiotic bacteria i.e. Lactobacillus acidophilus (A), Bifidobacterium longum (B), and Lactobacillus casei (C). The cultures of two probiotic bacteria AB and BC had been developed as well as three probiotic bacteria ABC culture. The mixed cultures (AB, BC and ABC) were prepared in single culture then cultivated in mixed culture as total 10% v/v of mare’s milk used and was incubated at 39°C for 9 h. The pH, acidity and bacterial count, each of them was analyzed in every 3 h of incubation time, while the organic acid and sensory tests were conducted at the end of 9 h of incubation. The results showed that the growth of mixed probiotic bacteria culture BC and ABC had better pH value that was around 3 compared with AB around 4.5. This showed that the cooperation between bacteria was different in each different combinations. The number of bacteria also increased sharply between 3-6 h of incubation time and 6-9 incubation time (AB and BC) along with the increased lactic acid, but the acetic acid decreased from 1750 mg/L to 1500 mg/L. The result of sensory test showed low acceptability of trained panelists. It is concluded that mixed cultures, using two or three probiotic bacteria (L. acidophilus, B. longum, and L. casei) could grow in mare’s milk. The change of the biochemical patterns indicated a commensalism cooperation among bacteria used. It was therefore the fermented mare’s milk using these probiotic bacteria were not well accepted by entrained panelists.
Mengidentifikasi Peptida Bioaktif Angiotensin Converting Enzyme-inhibitor (ACEi) dari Kasein β Susu Kambing dengan Polimorfismenya Melalui Teknik In Silico Widodo, Hermawan Setyo; Murti, Tridjoko Wisnu; Agus, Ali; Widodo, Widodo
Jurnal Aplikasi Teknologi Pangan Vol 7, No 4 (2018): November 2018
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.952 KB) | DOI: 10.17728/jatp.3008

Abstract

Susu kambing memiliki komponen protein salah satunya protein β dan secara umum terjadi polimorfisme pada level protein. Perubahan urutan asam amino akibat polimorfisme memungkinkan adanya potensi dihasilkannya peptida bioaktif penghambat enzim pengubah angiotensin (ACEi). Penelitian ini bertujuan untuk menyaring peptida bioaktif yang berpotensi sebagai ACEi dari kasein β kambing beserta polimorfismenya. Penelitian ini dilakukan dengan teknik in silico terhadap sekuen kasein β kambing serta struktur tiga dimensi human testicular ACE. Langkah yang dilakukan dalam penelitian ini meliputi simulasi pemotongan peptida dengan enzim pencernaan (pepsin, tripsin dan kimotripsin), peninjauan karakteristik peptida lalu simulasi docking ligan-reseptor. Tampilan parameter Lipinski’s Rule of Five (Ro5), bioaktivitas dan energi afinitas dipertimbangkan untuk memilih peptida bioaktif. Hasil yang didapat menunjukkan bahwa ditemukan peptida bioaktif yakni INK (Ile-Asp-Lys) yang memiliki kemampuan hampir setara dengan lisinopril (afinitas energi -8,2kkal/mol vs. -8,3kkal/mol). Peptida INK dapat ditemukan dari hasil hidrolisis dari alel A, C, D dan E, sehingga polimorfisme tidak menyebabkan perbedaan produksi peptida bioaktif. Kesimpulan yang dapat diambil yakni kasein β susu kambing jika dicerna dengan enzim pencernaan dapat menghasilkan peptida bioaktif ACEi yakni INK.Identification of Angiotensin Converting Enzyme-inhibitor (ACEi) Bioactive Peptide from Goat Milk β-Casein with It's Polymorphism by In Silico TechniqueAbstractPolymorphism eventually may be occurred at the protein level. Changes in the amino acid sequence due to polymorphism may exhibit a potential action to generate of the angiotensin-converting enzyme inhibitors (ACEi) bioactive peptide. This study is aimed to assess bioactive peptides that have a great potent value as ACEi from goat β casein along with its polymorphism. The research was done by in silico technique on goat β-casein sequence and three-dimensional structure human testicular ACE. Peptide-cutting simulations with digestive enzymes (pepsin, trypsin and chymotrypsin), peptide properties review, then ligand-receptor docking simulations was applied in this research. Appearance of Lipinski's Rule of Five (Ro5), bioactivity and affinity energy were considered for selecting bioactive peptides. The results show that bioactive peptide found as INK (Ile-Asp-Lys) which had similar ability as lisinopril (energy affinity –8.2kcal/mol vs. –8.3kcal/mol). The INK peptides could be found from the hydrolysis resulted in alleles A, C, D and E, therefore polymorphism did not affect the differences of production of bioactive peptides. A conclusion, processed goat milk β casein with digestive enzymes could produce ACEi of INK as bioactive peptide.