Lily Natalia
Unknown Affiliation

Published : 46 Documents
Articles

The Control of Anthrax Disease: Diagnosis, Vaccination and Investigation Adji, Rahmat Setya; Natalia, Lily
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.994 KB) | DOI: 10.14334/wartazoa.v16i4.841

Abstract

Anthrax is a bacterial disease caused by Bacillus anthracis attacking both animal and human (zoonosis) . The disease is normally associated with domestic livestock such as sheep, goats, and cattle, but humans are also infected due to exposure or comsuming infected animals . The control of anthrax in humans and animals involves developing a diagnostic method for B. anthracis detection and confirmation of anthrax, prevention by vaccines, and disease investigation . Rapid and more accurate diagnosis techniques for anthrax should be developed for improving the conventional method used in Indonesia . Vaccines are effective against anthrax . Current anthrax vaccine used in Indonesia is spores vaccine produced from a non-encapsulated, toxigenic. Sterne strain 34F2 of B. anthracis . The use of this vaccine occasionally causes local pain, necroses at the inoculation site, subcutaneous oedema and occasionally death of the animal . Several vaccines have been developed recently such as sub unit vaccine, anthrax vaccine absorbed (AVA), that contains a protective antigen (PA) component of the anthrax toxin as the major protective immunogen and is usually used in humans. In endemic areas of anthrax, outbreaks still routinely occur almost yearly . Monitoring of the epidemiological patterns of the disease has to be carried out by field investigation . Key words: Anthrax, Bacillus anthracis, zoonotic disease, disease control
Clostridial Necrotic Enteritis in Poultry Natalia, Lily
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 14, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1192.637 KB) | DOI: 10.14334/wartazoa.v14i4.795

Abstract

Necrotic enteritis caused by Clostridium perfringens type A and C has been found in poultry flocks in Indonesia or throughout the world for many years. The disease is sometimes not recognized and often underestimated. Clostridium perfringens normally live harmlessly in the gut or intestines of healthy chickens, but a trigger factor that tips up the balance in favour of the clostridia) bacteria, allowing them to proliferate, produce toxin and cause the disease. Proliferation of Cl. perfringens or production of the alpha toxin may be enhanced by component present in chicken diets, or the diets may inactivate infestinal digestive enzymes, thereby decreasing the degradation of alpha toxin. In poultry, the disease is manifested as haemorrhagic and necrotic lesions in the gut wall, cholangiohepatitis, as well as increased mortality. Beside the clinical manifestation, subclinical clostridia) necrotic enteritis associated with impaired feed conversion and retarded or poor growth is usually found in the field. A number factors predisposing to the development of necrotic enteritis are physical factors which damage the gut lining (coccidiosis, intestinal worms, etc.), feed composition, changes in nutrient density or protein levels, and immunosuppresion which reduces resistance to gut infections . Impaired feed conversion, reduced live weight at slaughter and increased condemnation percentage were major cases of production losses associated with necrotic enteritis. Presently, the prevalence of necrotic enteritis appears to be increasing and pose a serious health problem which is responsible for significant economic losses . Prevention of necrotic enteritis requires a delicate balancing act to control those factors that collectively are responsible for the disease. Antibiotic, prebiotic, competitive exclusion, enzyme preparation, imunisation, mineral and vitamin supplements have been used in poultry for controlling necrotic enteritis. Key words: Clostridial necrotic enteritis, Clostridium perfringens, toxin, chicken
Evaluation of antibody responses of cattle and buffaloes to Clostridium perfringens type A vaccine using ELISA Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 1, No 3 (1995)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.282 KB) | DOI: 10.14334/jitv.v1i3.33

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to detect specific antitoxin Clostridium perfringens type A. It was based on the use of purified alpha toxin of Clostridium perfringens type A as the coating antigen, which was then linked to specific alpha antitoxin . Horse raddish peroxidase labelled IgG was used as the conjugate, and 2,2-azino-bis (3- ethylbenzthiazoline-6-sulfonic acid) (ABTS) as the substrate . The ELISA was used to evaluate vaccination results on cattle and buffaloes against enterotoxaemia caused by Clostridium perfringens type A. Key words: ELISA, Clostridium perfringens type A antitoxin, vaccination
Prevention of enterotoxacmiu in transported cattle Natalia, Lily; ., Sudarisman; Darodjat, M
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 1 (1996)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (582.463 KB) | DOI: 10.14334/jitv.v2i1.45

Abstract

Fatal enterotoxaemia of transported cattle is frequently reported in Indonesiu. Acute enteritis and fatal enterotoxaemia of cattle and buffaloes in Indonesia are associated with toxigenic Clostridium perfringenr type A. The outbreaks could have been caused by some kinds of stress, such as a possible change in nutrition or management as well as transportation . To reduce mortality rate caused by enterotoxaemia, an effective vaccine against the disease was produced . The vaccine was made in an alum precipitated toxoid form, prepared from Clostridlumperfringens type A  toxin, which was then tested for safety in mice and for its capacity in generating high immunity in cattle. The vaccine was then used to immunise transported cattle as an attempt to reduce mortality rate and to observe antibody response of cattle following vaocletdon. The results showed that mortality in vaccinated was lower than in non-vaccinated groups of cattle. From field observation, it was obvious that alum precipitated toxoid vaccine could produce good immune response against enterotoxaemia in cattle . It was also evidence that this vaccine could reduce mortality in transported cattle .   Keywords: Enterotoxaemia, vaccine, transportation, cattle
Development of ELISA technique for detecting Clostridium novyi alpha toxin Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 3 (1997)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v2i3.69

Abstract

the investigation of infectious necrotic hepatitis . Modification of the indirect ELISA technique in the system used is an inhibition ELISA for antigen . The test samples were liver fluid from dead animals suspected from infectious necrotic hepatitis. To analyse for alpha toxin, microtiter plates were coated with antigen or alpha toxin . The liver fluid sample thought to contain antigen or toxin was then mixed together with reference antiserum containing specific alpha antitoxin of Cl. novyi. Enzyme labelled antiglobulin was then added, followed by enzyme substrate . The difference in colour change between a reference sample containing no antigen or toxin and the test sample solution indicates the amount of antigen or toxin in the test samples . This is a competitive assay; high toxin concentrations result in less colour at the end of the test . The sandwich ELISA technique was sensitive enough to detect as little as 390 ng/ml toxin in liver fluid sample. These results indicate that the ELISA technique is useful for detecting alpha toxin of Cl. novyi and for diagnosing infectious necrotic hepatitis .   Keywords: ELISA technique, Clostridium novyi alpha toxin, infectious necrotic hepatitis
Clostridial Necrotic Enteritis in Poultry Natalia, Lily
Indonesian Bulletin of Animal and Veterinary Sciences Vol 14, No 4 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1192.637 KB) | DOI: 10.14334/wartazoa.v14i4.795

Abstract

Necrotic enteritis caused by Clostridium perfringens type A and C has been found in poultry flocks in Indonesia or throughout the world for many years. The disease is sometimes not recognized and often underestimated. Clostridium perfringens normally live harmlessly in the gut or intestines of healthy chickens, but a trigger factor that tips up the balance in favour of the clostridia) bacteria, allowing them to proliferate, produce toxin and cause the disease. Proliferation of Cl. perfringens or production of the alpha toxin may be enhanced by component present in chicken diets, or the diets may inactivate infestinal digestive enzymes, thereby decreasing the degradation of alpha toxin. In poultry, the disease is manifested as haemorrhagic and necrotic lesions in the gut wall, cholangiohepatitis, as well as increased mortality. Beside the clinical manifestation, subclinical clostridia) necrotic enteritis associated with impaired feed conversion and retarded or poor growth is usually found in the field. A number factors predisposing to the development of necrotic enteritis are physical factors which damage the gut lining (coccidiosis, intestinal worms, etc.), feed composition, changes in nutrient density or protein levels, and immunosuppresion which reduces resistance to gut infections . Impaired feed conversion, reduced live weight at slaughter and increased condemnation percentage were major cases of production losses associated with necrotic enteritis. Presently, the prevalence of necrotic enteritis appears to be increasing and pose a serious health problem which is responsible for significant economic losses . Prevention of necrotic enteritis requires a delicate balancing act to control those factors that collectively are responsible for the disease. Antibiotic, prebiotic, competitive exclusion, enzyme preparation, imunisation, mineral and vitamin supplements have been used in poultry for controlling necrotic enteritis. Key words: Clostridial necrotic enteritis, Clostridium perfringens, toxin, chicken
The use of filter paper as a transport device for serology of Pasteurella multocida infection : Analysis and comparison ofprotein composition of filter paper extract and serum Natalia, Lily; Priadi, Adin
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (573.496 KB) | DOI: 10.14334/jitv.v3i3.115

Abstract

Two methods for collecting blood specimens for measuring antibody to Pasteurella multocida were compared. Blood was collected on filter-paper strips, air-dried and stored at 4°C along with paired samples collected by venepumeture . Analysis using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the protein composition of filter paper extract and serum was similar. Both samples had common proteins of 67, 52-58 and 27 kDa. However, there are two proteins bands of 14 and 30 kDa that were only found in, filter-paper extract. Westernblot analysis also showed that samples from both sampling techniques reacted to P. multocida proteins of 43 kDa. Samples from experimental and field animals were also collected by the two techniques and assayed by enzyme-linked immunosorbent assay (ELISA) for P. multocida antibodies . The agreement between samples from experimental animals and the field using ELISA was analyzed . Samples from experimental animals, showed a very high correlation (r = 0.931) in ELISA results among samples collected by the two techniques. However, the correlation was lower (r = 0.799) in samples collected from the field. Cost analysis showed that filter-paper collection technique was 100 times more economical compared to venepuncture technique. It was concluded that eluates of whole blood dried on filter paper can be used as an alternative to sera in ELISA for measuring antibodies to P. multocida.   Key words : Pasteurella multocida, serological tests, filter paper
Swamp Buffalo in South Kalimantan : Problem, Disease and Control Natalia, Lily; ., Suhardono; Priadi, Adin
Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1104.524 KB) | DOI: 10.14334/wartazoa.v16i4.842

Abstract

In recent years, several studies have been carried out to evaluate and investigate the important diseases of swamp buffaloes (Bubalus carabanensis) in Kalimantan . More attention has been focused on the case of acute infectious diseases and sudden death in the buffaloes . Fasciolosis black disease, acute enteritis, especially fatal enterotoxaemia haemorrhagic septicaemia . and trypanosomiasis (Surra), are some of the important diseases found in these animals . Black disease caused by toxigenic Clostridium novyi occurs in the presence of the organism in the liver and the degree of liver fluke Fasciola gigantica infestation . In regions where black disease is enzootic, Cl. novvi can be isolated from livers of normal healthy animals . In Hulu Sungai Utara district, South Kalimantan, the prevalence of fasciolosis caused by Fasciola gigantica in swamp buffalo was 77% in 1991 . A gross sudden change in diet due to seasonal changes could induce rumen and intestinal stasis, which provide a favourable environment for the rapid proliferation of commensal toxigenic Clostridium perfringens in the small intestine . Subsequent absorption of the toxin produced through the gut wall and its generalized dissemination culminated in a fatal enterotoxaemia . Haemorrhagic septicaemia (HS) is an acute, fatal disease affecting swamp buffalo, and caused by Pasteurella multocida B : 2 . The swamp buffalo is particularly susceptible for HS, and the reported greatest losses of swamp buffalo in Kalimantan due to HS is recorded in 1980s. The clinical signs of Surra in swamp buffalo were also found in certain areas in Danau Panggang area . Hulu Sungai Utara district . Vaccination is the accepted method for controlling Black disease, enterotoxaemia and HS. Multi component vaccine, alum adjuvant containing at least 5 types of clostridial toxoids and P. multocida B2 bacterin have been used and provide good protection to the animals . Control and treatment of liver fluke infestation is advisable. Vaccination is recommended annually and should be carried out regularly . In these days, the farmers concern about the availability of the grass in the Danau Panggang area . The primary feed grass (Oryza sativa forma spontanea L), of the swamp buffalo were not sufficient in the low-tide season. The over population of Pamocea canaliculata (golden snail), a pest for lake vegetations, was thought to be responsible for this phenomena . Growing forages as the source of animal feed may be an option to improve the current performance of the swamp buffalo in the area. Key words : Diseases, swamp buffalo, control, South Kalimantan
Pathogenesis of Haemorrhagic Septicaemia (HS) in cattle and buffalo: clinical signs, pathological changes, reisolation and detection of Pasteurella multocida using culture medium and Polymerase Chain Reaction (PCR} Priadi, Adin; Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 1 (2000)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.32 KB) | DOI: 10.14334/jitv.v5i1.181

Abstract

In the study of the pathogenesis of Haemorrhagic Septicaemia (HS), one cattle and one buffalo were infected subcutaneously with a dose of 4 x 108 colony forming units of Pasteurella multocida B:2 in the neck region. The post infection clinical findings were observed. During this observation period, bacterial isolation was carried out from heparinised blood and nasal swabs. The buffalo succumbed 2 hours earlier than the cattle.111e post mortem pathological changes in cattle and buffalo were similar but the lesions most severe in the buffalo. The prominent changes were observed in the lungs and bronchi of both animals. Bacterial reisolation and Polymerase Chain Reaction (PCR) for P. multocida were carried out trom various samples kept at room temperature without any preservative for 15, 35 and 59 hours after the death of the animals. After 59 hours, heavily contaminated samples were found in all organs except bone marrow. Reisolation of P. multocida trom these samples was difficult, however, the organism can still be identified by PCRTo improve the viability of Pasteurella multocida and reducing the growth of contaminants, transport medium containing selective antibiotics was developed. Amikacin and Gentamicin were good selective antibiotics to suppress other contaminating organisms.   Key words: Pathogenesis, Pasteurella multocida B:2, cattle and buffalo, selective medium, PCR
Latex agglutination test for field diagnosis of haemorrhagic septicaemia Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.833 KB) | DOI: 10.14334/jitv.v6i3.235

Abstract

Pasteurella multocida is bacterial pathogens that cause haemorrhagic septicaemia (HS) in cattle and buffaloes. Various tests have been used to differentiate types of P. multocida, as well as to diagnose this specific disease. A latex agglutination test has been developed for the detection of P. multocida B:2 which is the causal agent of HS. This test is a rapid and simple test suitable for local laboratorium to diagnose HS cases in the field. A heat stable antigen consisting of mainly lipopolysaccharide (LPS) extract of formalin killed P. multocida 0019 was used to produce specific antibody against P. multocida B:2. The antiboy was then used to sensitise latex particles. Latex agglutination test have been used to screen some P. multocida field isolates and this test have been proven to be specific, simple and easy to be used in detecting P. multocida B:2. The specificity is due to antibodies recognising LPS or LPS protein complexes. Sensitised latex was stable at 4° C for at least12 months. This test should be used as an aid to diagnosis and employed principally to confirm and support clinical and post mortem findings of HS.   Keywords: Pasteurella multocida B:2, haemorrhagic septicaemia (HS), diagnosis, latex agglutination test