Hasnah Natsir
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar 90245, South Sulawesi

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PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE GRACILARIA VERRUCOSA Muawanah, Muawanah; Ahmad, Ahyar; Natsir, Hasnah
Marina Chimica Acta Vol 18, No 2 (2017): Volume 18 No. 2 (2017)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v18i2.2688

Abstract

 it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation .Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and  the crude extract and fraction  4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.
ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE EUCHEUMA COTTONII AND EUCHEUMA SPINOSUM Muawanah, Muawanah; Ahmad, Ahyar; Natsir, Hasnah
Marina Chimica Acta Vol 17, No 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.837 KB) | DOI: 10.20956/mca.v17i2.1123

Abstract

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent.
PRODUCTION OF PROTEASE ENZYME FROM BACTERIA IN HOT SPRING OF SOUTH SULAWESI, BACILLUS LICHENIFORMIS HSA3-1A Natsir, Hasnah
Marina Chimica Acta Vol 16, No 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.315 KB) | DOI: 10.20956/mca.v16i1.949

Abstract

Bacillus licheniformis HSA3-1a is able to produce extracellular protease. The aim of this research was to find out its production optimum time, consentration of MnCl2, temperature and optimum pH, also the cofactor influence on activity protease from B. licheniformis HSA3-1a. The protease activity was tested with a modified method of Walter. Protease production time was optimum for 36 hours with an activity value of 0,394 U/mL, protein content was 132,35 mg/mL. The Optimum condition of protease enzyme were pH 7,0 and temperature 45 ?C with an activity value of 0,0463 U/mL. Protease activity could be inhibited by MnCl2 concentration of 0,01 - 0,03 M.
PRODUCTION AND APPLICATION OF CHITIN DEACETYLASE FROM BACILLUS LICHENIFORMIS HSA3-1A AS BIOTERMITICIDE Ischaidar, Ischaidar; Natsir, Hasnah; Dali, Seniwati
Marina Chimica Acta Vol 15, No 1 (2014): Volume 15 No. 1 (2014)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1025.64 KB) | DOI: 10.20956/mca.v15i1.969

Abstract

BIOACTIVITY OF PROTEIN FRACTION IN BROWN ALGAE, TURBINARIA DECURRENS, AS ANTIBACTERIAL AGENT BIOAKTIVITAS FRAKSI PROTEIN DALAM ALGA COKLAT, TURBINARIA DECURRENS, SEBAGAI AGEN ANTI BAKTERI Dali, Seniwati; Natsir, Hasnah; Usman, Hanapi; Ahmad, Ahyar
Marina Chimica Acta Vol 14, No 1 (2013): Volume 14 No. 1 (2013)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.944 KB) | DOI: 10.20956/mca.v14i1.2930

Abstract

Penelitian ini bermaksud mengeksplorasi dan mengkarakterisasi fraksi protein dari alga cokelat Turbinaria decurrens di Sulawesi Selatan. Senyawa protein bioaktif diperoleh melalui serangkaian proses ekstraksi, fraksionasi dengan kejenuhan amonium sulfat 0-20%, 20-40%, 40-60%, dan 60-80% yang dilanjutkan dengan proses dialisis lalu ditentukan kadar proteinnya dengan metode Lowry. Fraksi protein yang diperoleh diuji bioaktivitasnya terhadap bakteri Staphylococcus aureus dan Salmonella thypi dengan metode difusi agar pada medium MHA. Hasil penelitian menunjukkan bahwa kadar protein tertinggi ditemukan pada fraksi 0-20% yaitu sebesar 5,1 mg/mL. Hasil uji bioaktivitas antibakteri terbesar ditemukan pada fraksi protein 0-20% terhadap Staphylococcus aureus dan Salmonella thypi dengan zona daerah hambatan masing-masing sebesar 18,33 mm dan 13,30 mm yang bersifat bakteriostatik. Hasil penelitian awal ini akan dikembangkan pada cakupan penelitian yang lebih luas untuk mengkloning protein bioaktifnya dan uji pre-klinis dari protein antibakteri yang diperoleh.
THE EFFECT OF FE2+ DAN MN2+ IONS TOWARD β-CAROTENE PRODUCTIVITY BY PHYTOPLANKTON ISOCHRYSIS AFF GALBANA (T-ISO) Mayasari, Erna; Raya, Indah; Natsir, Hasnah
Marina Chimica Acta Vol 13, No 2 (2012): Volume 13 No. 2 (2012)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.29 KB) | DOI: 10.20956/mca.v13i2.1194

Abstract

The research aimed to find out the effect of Fe2+ and Mn2+ towards ?-carotene productivity as result of oxidative stress from photosystem (PS II). The phytoplanton Isocrysis aff galbana (T-iso) were the microalgae species which had the high lipid content primarily and had potentiality to produce ?-carotene as the ?-carotene supplement. Analysis method was carried out by the sonication extraction for short and cheap in the lysis cell of phytoplankton biomassa, infra red (IR) to find out the interaction metal ion and UV/VIS spectrophotometer to determine ?-carotene concentration. The research result indicateds that Fe2+ has higher impact than Mn2+ on the ?-carotene productivity. The interaction of ions metal indicates as   M-N, -O-M and M?OH-C complex in the phytoplankton amino acid. The ?-carotene concentration is 4,97 µg/g DW in addition Fe2+ 0,30 ppm and 1,95 ?g/g DW in Mn2+  0,20 ppm. The dry weight concentrations of ?-carotene indicates that  I. aff galbana with Fe2+ addition has potential as ?-carotene supplement. 
ENZYMATIC PRODUCTION OF CHITOSAN FROM THE WASTE WHITE SHRIMP (PENAEUS MERGUIENSIS) AND ANTIMICROBIAL EFFECT FOR DURABILITY AGAINST FISHBALLS Arif, Abdur Rahman; Natsir, Hasnah; Dali, Seniwati
Marina Chimica Acta Vol 15, No 1 (2014): Volume 15 No. 1 (2014)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1187.393 KB) | DOI: 10.20956/mca.v15i1.965

Abstract

PRODUKSI OLIGOMER KITOSAN DARI LIMBAH UDANG WINDU (PANAEUS MONODON) MENGGUNAKAN ENZIM KITOSANASE DARI ISOLAT BAKTERI KLEBSIELLA SP. Sarni, Sarni; Natsir, Hasnah; Dali, Seniwati
Indonesian Journal of Chemical Research Vol 3 No 2 (2016): Edisi Bulan Januari (Edition for January)
Publisher : Chemistry Department, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to ?- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine  are formed through a process of severing ties depolymerization of chitosan with ?-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which  soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).
STUDY OF COMPOUNDS FROM EXTRACT OF Melochia umbellata (Houtt.) Stapf var. Degrabrata K. (PALIASA) LEAVES THAT HAS POTENTIAL AS ANTIBACTERIAL Wullur, Selfi; Firdaus, Firdaus; Natsir, Hasnah; Hariani Soekamto, Nunuk
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Volume 8 No 1 - June 2015
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ica.v8i1.2472

Abstract

Determination of antibacterial activity of each extract of M. umbellata (Houtt.) Stapf var. K. degrabrata leaves has been conducted against three strains bacteria Escherichia coli, Staphylococcus aureus, and Shigella dysenteriae by using amoxicillin as a positive control and DMSO as a negative control. Agar diffusion method has been used with MHA medium (Muller Hinton Agar) on variation concentrations of each extract were 2500 ppm, 5000 ppm, 10,000 ppm, and 20,000 ppm N-hexane extract was the most active extract against S. aureus with zone of inhibitions were 10.50 mm, 10.80 mm, 11.00 mm and 11.45 mm, respectively. The most active extract against S. dysenteriae was ethyl acetate extracts with zone of inhibitions were 9.36 mm, 11.55 mm, 11.58 mm and 17.70 mm, respectively. Isolated compound from n-hexane extract was first compound as white powder with a melting point of 75-77 °C which was identified as 1-Tetracosanol and second compound was obtained in the form of a yellow paste which was identified as steroid compound that had long conjugated substituent. These compounds were elucidated based on the data of IR, UV-Vis, 1H-NMR, and 13C-NMR.
Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp. Sarni, Sarni; Natsir, Hasnah; Dali, Seniwati
Indo. J. Chem. Res. Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.051 KB)

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine  are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which  soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).