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Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah, Parawansah; Nuralifah, Nuralifah; Alam, Gemini; Natzir, Rosdiana
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.194

Abstract

BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
NURSES’ PERCEPTIONS AND EXPECTATIONS ON THE IMPLEMENTATION OF CAREER LADDER IN PUBLIC HOSPITALS IN MAKASSAR Pasang, Meyke Tiku; Kadar, Kusrini; Natzir, Rosdiana
Indonesian Contemporary Nursing Journal (ICON Journal) Volume 2 No. 1 Agustus 2017 - Januari 2018
Publisher : Fakultas Keperawatan Universitas Hasanuddin

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Abstract

Introduction: Nurses as professionals must provide services in accordance with the competence and authority possessed either individually or as part of a hospital team. Thus, there is a need to manage the resources of nursing  such as the professional career development system for nurses. The study aimed to identify the perception and expetation of the nurses on the implementation of career ladder in two hospitals in Makassar city. Method: This research used quantitative method with the cross sectional design. There were 105 nurses from Hasanuddin University Hospital and 212 nurses from Dr. Wahidin Sudirohusodo Hospital involved as samples in this study. The samples were chosen using the disproportionate stratified random sampling technique. The data were collected through questionnaires to find out the nurse perception including some open ended  question to explore the nurses expectation from this career ladder program.  The data were  analyzed using descriptive statistic and mann-whitney to seee the differences perception between the groups. Result: The result revealed although that mean of perception about the implementation of career ladder in two hospitals are considered good (m=3.70 dan m=3.94), there was differences of nurses perception in both hospitals (p=0.00). Conclusions: Meanwhile, the result of open-ended questions about nurses expectation about the implementation of the career ladder emphasized that there should  be a schedule for training for their professional improvement. Evaluation for further assessment for higher levels of career ladder also considered important for the nurses in both hospitals. Furthermore, the rewards or incentives for the nurses received shold be appropriate according to the career levels. In conclusion, the implementation of career ladder program in both hospitals have been considered good, however, the hospitals need to support the nurses and pay attention to factors that could influence the nurses satisfaction of the program.
CORRELATION BETWEEN COMPENSATION AND WORK SATISFACTION WITH NURSES’ PERFORMANCE THROUGH MOTIVATION IN BETHESDA PUBLIC HOSPITAL OF TOMOHON Kalalo, Chyntia Novita; Sjattar, Elly Lilianty; Natzir, Rosdiana
Indonesian Contemporary Nursing Journal (ICON Journal) Volume 3 No. 1 Agustus 2018 - Januari 2019
Publisher : Fakultas Keperawatan Universitas Hasanuddin

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Abstract

Introduction: Compensation provided by hospital and job satisfaction of nurses can affect the performance of nurses. The research aimed to analyze the correlation between compensation and work satisfaction with nurses’ performance through motivation in inpatient room of Bethesda Publik Hospital of Tomohon. Method: The method used is analytic observational approach with cross sectional study. The number of sample were of 50 respondents. The methods of obtaining the data were questionnaire with Likert scale and observation of nurses’ performance with Guttman scale. The data were analyzed using univariate and bivariate analysis by means of frequency distribution table and cross tabulation with chi square. Results: The research indicate that compensation has a correlation with work load (p=0.003), motivation (p=0.040), and nurses’ performance (p=0.001). Work satisfaction has a correlation with motivation (p=0.002) and nurses’ performance (p=0.000). Motivation has a correlation with nurses’ performance (p=0.013). Conclusion: The management of the hospital should pay attention to compensation given and nurses’ work satisfaction, so the nurses are more motivated to carry out services in a professional nursing care in order that more optimal nurses’ performance.
ELEVATED UNCONJUGATED BILIRUBIN IN SCHIZOPHRENIC PATIENTS Aziz, Ilhamuddin; Widaningsih, Yuyun; Natzir, Rosdiana; Tanra, A. Jayalangkara
Nusantara Medical Science Journal Volume 3 No. 1 Januari - Juni 2018
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v3i1.5780

Abstract

Strategies for schizophrenic treatment still have been developed in order to increase their effectively, based on latest findings in the etio-pathology. Coincidence of hyperbilirubinemia, especially related to Gilbert’s Syndrome (GS), and schizophrenia/other psychiatric disorders, was reported in several studies although the pattern of this alteration is still controversial.  Bilirubin could induce microglia to release pro-inflammatory cytokine that cause neuroinflammation, one of hypothetic etio-pathogenesis has been the most extensively studied recently. However, no data have been presented about this phenomenon in Indonesia. Therefore, this study aims to investigate plasma bilirubin concentration in psychotic spectrum.This study is cross sectional design that we examined both the total bilirubin and direct bilirubin of every psychotic patient admitted for the first time to Rumah Sakit Khusus Daerah (RSKD) Provinsi Sulawesi Selatan over a period of July—December 2012, by using health people as control. Diagnostic determination was set based on International Classification of Diseases (ICD) 10. Patients suffering from any disease such as anemia (decreased hemoglobin), liver diseases (elevated liver enzymes), or substance abuse were excluded.            From 73 samples, we found that plasma UCB level was significantly elevated, higher in psychotic patients, especially schizophrenic group, than in the control. Total bilirubin rate was slightly increased in schizophrenic group compare to the others and two samples in this group showed hyperbilirubinemia. Further investigations are needed to ensure that whether the elevated UCB was related to GS and whether this condition has inflammatory effect.